Temat: biologiczne zwalczanie - Katalog OPAC zbiorów

Tytuł:: Przegląd metod zwalczania szkodliwych stawonogów, w tym o znaczeniu medycznym i weterynaryjnym
Survey of methods controlling arthropod pests of sanitary and veterinary importance
Autorzy:: Sobotka, W.
Styczynska, B.
Powiązania:: https://bibliotekanauki.pl/articles/837280.pdf
Data publikacji:: 1991
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: stawonogi
owady
roztocze
kleszcze
organizmy szkodliwe
wektory chorob
zagrozenia zwierzat
zagrozenia czlowieka
zwalczanie szkodnikow
metody zwalczania szkodnikow
metody chemiczne
srodki chemiczne
substancje semiochemiczne
metody biologiczne
biopreparaty
wrogowie naturalni
Opis:: Chemical and biological methods of the title arthropod pests control, during the past 1985-1989, have been reviewed. Among insecticides the chlorinated hydrocarbons, organophosphorous compounds, carbamates and particularly pyrethroides played still the outstanding role. Juvenoids, chitin synthesis inhibitors and semiochemicals such as pheromones and feeding deterrents were discussed. WHO recommended insecticides for mosquito, housefly, cockroach Pharaoh's ant, flea, and bedbug control in Poland have been listed. Some biological methods applied against agricultural pests were mentioned.
Źródło:: Annals of Parasitology; 1991, 37, 1
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

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Tytuł:: Przegląd metod zwalczania szkodliwych stawonogów, w tym o znaczeniu medycznym i weterynaryjnym
Survey of methods controlling arthropod pests of sanitary and veterinary importance
Autorzy:: Sobotka, W.
Styczynska, B.
Powiązania:: https://bibliotekanauki.pl/articles/2152318.pdf
Data publikacji:: 1991
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: stawonogi
owady
roztocze
kleszcze
organizmy szkodliwe
wektory chorob
zagrozenia zwierzat
zagrozenia czlowieka
zwalczanie szkodnikow
metody zwalczania szkodnikow
metody chemiczne
srodki chemiczne
substancje semiochemiczne
metody biologiczne
biopreparaty
wrogowie naturalni
Opis:: Chemical and biological methods of the title arthropod pests control, during the past 1985-1989, have been reviewed. Among insecticides the chlorinated hydrocarbons, organophosphorous compounds, carbamates and particularly pyrethroides played still the outstanding role. Juvenoids, chitin synthesis inhibitors and semiochemicals such as pheromones and feeding deterrents were discussed. WHO recommended insecticides for mosquito, housefly, cockroach Pharaoh's ant, flea, and bedbug control in Poland have been listed. Some biological methods applied against agricultural pests were mentioned.
Źródło:: Wiadomości Parazytologiczne; 1991, 37, 1; 167-171
0043-5163
Pojawia się w:: Wiadomości Parazytologiczne
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Charakterystyka enzymow proteo-, chityno- i lipolitycznych pasozytniczego grzyba Conidiobolus coronatus
Characterization of proteo-, chitino- and lipolytic enzymes of parasitic fungus Conidiobolus coronatus
Autorzy:: Wloka, E
Powiązania:: https://bibliotekanauki.pl/articles/836974.pdf
Data publikacji:: 2010
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: grzyby pasozytnicze
grzyby entomopatogenne
Conidiobolus coronatus
Entomophthorales
enzymy proteolityczne
elastaza
N-acetyloglukozaminidaza
enzymy chitynolityczne
enzymy lipolityczne
lipaza
aktywnosc enzymatyczna
owady
barciak wiekszy
Galleria mellonella
zwalczanie szkodnikow
metody biologiczne
grzyby owadobojcze
Źródło:: Annals of Parasitology; 2010, 56, 1; 83-85
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

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Tytuł:: Charakterystyka enzymów proteo-, chityno- i lipolitycznych pasożytniczego grzyba Conidiobolus coronatus
Characterization of proteo-, chitino- and lipolytic enzymes of parasitic fungus Conidiobolus coronatus
Autorzy:: Włóka, E
Powiązania:: https://bibliotekanauki.pl/articles/2143413.pdf
Data publikacji:: 2010
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: grzyby pasozytnicze
grzyby entomopatogenne
Conidiobolus coronatus
Entomophthorales
enzymy proteolityczne
elastaza
N-acetyloglukozaminidaza
enzymy chitynolityczne
enzymy lipolityczne
lipaza
aktywnosc enzymatyczna
owady
barciak wiekszy
Galleria mellonella
zwalczanie szkodnikow
metody biologiczne
grzyby owadobojcze
Opis:: The largest problem in limitation of insect pest population is increasing resistance of them to chemical pesticides. Alternative are entomopathogens, which regulate frequency of insect pests. Among them decisive role play entomopathogenic fungi, which possess the ability to active penetration through cuticle by mechanical pressure of invasive hypha and production of proteo-, chitino- (egzo- and endochitinases) as well as lipolytic enzymes, which provide nutrients for subsequent development of fungus. Entomopathogenic soil fungus Conidiobolus coronatus (Entomophtorales) is saprophyte fungus, which demonstrates a high efficiency in the paralysis of varied insects. Although leading investigations over mechanism of insect paralysis, we still do not know, what role fungal enzymes play in insect cuticle penetration. The main aim of research was establishment of optimal conditions for elastase, N-acetylglucosaminidase (NAGase), chitobiosidase as well as lipase. Optimal reaction parameters were determined: volume of reaction mixture, volume of homogenate, working pH and the substrate concentration. Having on aim a possible use of C. coronatus in pest control, two ranges of temperatures were chosen: 20°C – optimal temperature for the fungus growing and 30°C – optimal temperature for the cultivation of the great wax moth larvae, Galleria mellonella, on which examinations were performed. Also kinetic constants Km and Vmax were determined. Activity of elastase and N-acetylglucosaminidase of C. coronatus was measured spectrophotometrically at 410 nm (towards N-Succinyl-Ala-Ala-Pro-Leu-p-Nitroanilide) and 405 nm (towards 4-Nitrophenyl-N-acetyl-b-D-glucosaminide), respectively. The following optimal conditions of elastase activity were established: the volume of reaction mixture 0.5 ml, volume of homogenate 1 ml, temperature 30°C, pH 8, substrate concentration 40 mM. Optimal conditions of NAGase assay: the volume of reaction mixture 0.5 ml, dose of homogenate 12.5 ml, temperature 30°C, pH neutral and 6 mM substrate concentration. The activities of chitobiosidase and lipase were measured spectrofluorometrically (Ex=360 nm, Em=450 nm) towards 4-Methylumbelliferyl b-D-N-N’-diacetylchitobioside and 4-Methylumbelliferyl oleate, respectively. Chitobiosidase showed the highest activity in dose of 30 ml in 1 ml volume of reaction mixture, at the temperature of 30°C, pH 7 and substrate concentration equal to 2 mM. Lipase showed the highest catalytic activity in 1 ml volume of reaction mixture, in 30°C but 50 ml of homogenate, pH 10 and 10 mM substrate concentration were needed. Higher activity investigated enzymes in 30°C than 20°C indicated that they can take part in pathogenesis. It was suggested that as first in perforation of coats of insects body elastase and lipase take part. Indicated of it, large thermoresistance of both enzymes (only 10.5% decrease of elastase activity at 20°C and 9.4% decrease of lipase activity in comparison with maximal activity at 30°C), alkalophilicity of both proteins (elastase shows the alkaline optimal pH equal to 8 at pH 9 preserves 97% activity, and at pH 10 94% activity, respectively while lipase prefers the pH 10 and at pH 8 and pH 9 enzyme keeps 57 and 60% activity, respectively) as well as lack of repression by suitable substrates. Sigmoid character of curve concerning pH influence on the activity of both enzymes, also indicates similarity between elastase and lipase. On minor part of NAGase and chitobiosidase of fungus C. coronatus in perforation of coats of host body showed high sensibility of both enzymes on hydrogen ions concentration: both enzymes prefer neutral pH, in pH 6 and 8 lose over 35% activity but subjection to substrate repression and 3–4-fold growth of activity followed only in 30°C. In the course of work it was found, that rich medium (LB) stimulates growth of mycelium and production of fungal lipases. So far nobody managed to isolate chitinolytic or lipolytic enzymes from C. coronatus homogenate. The majority of fungal enzymes were isolated from post incubation filtrates. In the literature of the subject lack of data about C. coronatus NAGase, therefore in examinations also the trial of isolation NAGase from C. coronatus homogenate was undertaken. Activity of NAGase showed only first fraction, which did not separate with none of used columns. Disappointing results of purification on cation exchanger CM, weak anion exchanger DEAE, and strong anion exchanger Q were obtained as well as after fractionation tests with the use of Microcon microcolumns. In aim of NAGase molecular mass estimation, two zymograms were made with Triton X-100 and casein and with the use of fluorescent substrate 4-Methylumbelliferyl N-acetyl-b-D-glucosaminide. Molecular mass of NAGase from C. coronatus was established on ca. 60 kDa. This is the first report describing molecular weight of NAGase from C. coronatus. Examined NAGase has different properties than known NAGases from other entomopathogenic fungi. Although its molecular weight is equal to the Metarhizium anisopliae NAGase, optimal pH for both NAGases are different: neutral in the case of C. coronatus NAGase versus acidic in the case of M. anisopliae NAGase. Knowledge of molecular mass of the C. coronatus NAGase should allow to find a new method of this enzyme isolation from C. coronatus homogenate. Thanks to developed methods of assaying activities of elastase, NAGase, chitobiosidase and lipase, real becomes the understanding of mechanism of insects paralysis through C. coronatus fungus.
Źródło:: Wiadomości Parazytologiczne; 2010, 56, 1; 83-85
0043-5163
Pojawia się w:: Wiadomości Parazytologiczne
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Możliwości ochrony lasu przed owadami uszkadzającymi systemy korzeniowe metodą biologiczną z wykorzystaniem grzybów entomopatogennych. I. Mechanizm zakażania owadów grzybami entomopatogennymi i czynniki wpływające na infekcję
Possibility of forest protection against insects damaging root systems with the use of biological method based on entomopathogenic fungi. I. Mechanism of insects infection with entomopathogenic fungi and factors...
Autorzy:: Malinowski, H.
Powiązania:: https://bibliotekanauki.pl/articles/1009635.pdf
Data publikacji:: 2009
Wydawca:: Polskie Towarzystwo Leśne
Tematy:: zwalczanie szkodnikow
grzyby entomopatogenne
biologiczne metody ochrony roslin
Metarhizium anisopliae
owady
szkodniki korzeni
ochrona lasu
lesnictwo
Verticillium lecanii
Beauveria brongniartii
insects infection
entomopathogenic fungi
beauveria brongniartii
metarhizium anisopliae
verticillium lecanii
insects control
Opis:: The paper comprises basic knowledge on the mechanism of insects infection with entomopathogenic fungi (Beauveria brongniartii, Metarhizium anisopliae, Verticillium lecanii) and factors influencing this process. It depends on pathogen properties, host susceptibility, and biotic and abiotic elements of environment. Such information is useful to understand the slower effectiveness of mycoinsecticides than chemical insecticides.
Źródło:: Sylwan; 2009, 153, 12; 795-804
0039-7660
Pojawia się w:: Sylwan
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Wrażliwość larw Hylobius abietis (L.) na rodzime gatunki i szczepy nicieni entomopatogenicznych
Sensitivity of Hylobius abietis (L.) larvae on native species and isolates of entomopathogenic nematodes
Autorzy:: Tumalis, D.
Skrzecz, I.
Mazurkiewicz, A.
Pezowicz, E.
Góral, K.
Powiązania:: https://bibliotekanauki.pl/articles/991620.pdf
Data publikacji:: 2013
Wydawca:: Polskie Towarzystwo Leśne
Tematy:: lesnictwo
ochrona lasu
szkodniki roslin
owady
szeliniak sosnowiec
Hylobius abietis
larwy
zwalczanie szkodnikow
biologiczne metody ochrony roslin
nicienie entomopatogenne
Steinernema feltiae
Steinernema kraussei
hylobius abietis
entomopathogenic nematodes
steinernema feltiae
steinernema kraussei
biological control agent
Opis:: The use of entomopathogenic nematodes (Steinernema and Heterorhabditis spp.), against the large pine weevil Hylobius abietis (L.) is currently studied in many countries. They show a great potential of nematodes for H. abietis control in laboratory and field experiments. In 2011 similar studies began in Poland to develop new biological control method of H. abietis with the use of native isolates of the nematodes. The first laboratory stage of the experiments pointed at the estimation of biological activity of 3 native isolates: 2 of Steinernema feltiae (Filipjev) and 1 of S. kraussei (Steiner). The tested nematodes were applied at the dose of 100 invasive larvae per 1 larva of H. abietis. After 48 hours H. abietis larvae were sectioned and examined for a determination of their mortality, extensivity and intensity of infection. No statistical differences were found between larvae mortality and extensivity of infection by tested isolates of nematodes. In all variants the mortality and extensivity of infection were high and reached 92−100% and 80−86% respectively. Statistical differences were found between the intensity of infection, which were the highest in case of S. feltiae Zag15. The obtained results indicated different activity of nematode isolates within one speies.
Źródło:: Sylwan; 2013, 157, 10; 769-774
0039-7660
Pojawia się w:: Sylwan
Dostawca treści:: Biblioteka Nauki