- Tytuł:
- Assessment of mutagenic activity of methyl- and phenylphenanthrenes based on Salmonella test and micronucleus test
- Autorzy:
-
Rudnicka, K.
Tejs, S.
Budzikur, K. A.
Mielżyńska-Švach, D.
Jakimiuk, E.
Chachaj, A.
Góra, M.
Żelazna, K.
Łuczyński, M. K. - Powiązania:
- https://bibliotekanauki.pl/articles/363178.pdf
- Data publikacji:
- 2013
- Wydawca:
- Uniwersytet Warmińsko-Mazurski w Olsztynie
- Tematy:
-
Ames test
in vitro
micronucleus test
mutagenicity
phenanthrene
test Amesa
mutagenność
test mikrojądrowy
fenanatren - Opis:
- Polycyclic aromatic hydrocarbons (PAHs) are widely spread environmental pollutants mainly originating from anthropogenic sources such as fossil fuel combustion, industries, and others. Although a large body of literature exists on the toxicity and carcinogenicity of PAHs, primarily benzo[a]pyrene, toxicity data for phenanthrene deriveratives are very limited. The main aim of the experiment was to investigate if there exists correlation between molecular structure and mutagenic activity of four phenanthrene derivatives: 1 methylphenanthrene, 4 methylphenanthrene, 1 phenylphenanthrene, and 4 phenylphenanthrene. An Ames assay using two strains of histidine dependent Salmonella Typhimurium (TA98 and TA100) was conducted to assess the mutagenic activity of studied compounds both in the presence (+S9) and in the absence (-S9) of an exogenous source of metabolic activation. The compounds were also tested in an in vitro chromosome aberration assay in which V-79 cells were exposed to the phenanthrene derivatives investigated both in the presence and in the absence of metabolic activation. The phenylphenanthrenes showed no mutagenic effect. These compounds occasionally induced significant decrease in the number of revertants in the Ames test. The greatest mutagenic effects were observed for 1 methylphenanthrene after metabolic activation (+S9). In the micronucleus test the greatest mutagenic effect was observed for 4 methylphenanthrene also in the presence of metabolic activation system. The results obtained are comparable to those reported earlier for the methylphenanthrenes.
- Źródło:
-
Environmental Biotechnology; 2013, 9, 2; 65-71
1734-4964 - Pojawia się w:
- Environmental Biotechnology
- Dostawca treści:
- Biblioteka Nauki
Artykuł