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    Wyświetlanie 1-14 z 14
    Tytuł:
    Mitochondrial DNA in pediatric leukemia patients
    Autorzy:
    Kodroń, Agata
    Ghanim, Magda
    Krawczyk, Katarzyna
    Stelmaszczyk-Emmel, Anna
    Tońska, Katarzyna
    Demkow, Urszula
    Bartnik, Ewa
    Powiązania:
    https://bibliotekanauki.pl/articles/1038705.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    pediatric ALL
    mtDNA
    chemotherapy
    Opis:
    Numerous studies of mitochondrial DNA (mtDNA) in cancer have shown differences between mtDNA sequences in tumor and normal tissue and at various stages of cancer treatment in the same patient. However, there is little data on acute lymphoblastic leukemia (ALL), the most common type of leukemia in children. In this study we compared mitochondrial sequence variation in the D-loop region and in 5 genes of mtDNA in bone marrow samples of 6 pediatric patients with ALL at various stages of therapy. We found several common polymorphisms and one variant at position 3688 whose level varied during leukemia treatment. Our results suggest that mitochondrial DNA mutations, whose levels change during patient treatment, could be potential biomarkers for monitoring treatment efficacy and disease progression.
    Źródło:
    Acta Biochimica Polonica; 2017, 64, 1; 183-187
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Role of mitochondria in carcinogenesis
    Autorzy:
    Tokarz, Paulina
    Blasiak, Janusz
    Powiązania:
    https://bibliotekanauki.pl/articles/1039194.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    cancer
    mitochondria
    mtDNA
    ROS
    anti-cancer therapy
    Opis:
    Mitochondria play the central role in supplying cells with ATP and are also the major source of reactive oxygen species (ROS) - molecules of both regulatory and destructive nature. Dysfunction of mitochondrial metabolism and/or morphology have been frequently reported in human cancers. This dysfunction can be associated with mitochondrial DNA (mtDNA) damage, which may be changed into mutations in mtDNA coding sequences, or the displacement-loop region, changes in the mtDNA copy number or mtDNA microsatellite instability. All these features are frequently associated with human cancers. Mutations in mtDNA can disturb the functioning of the ROS-producing organelle and further affect the entire cell which may contribute to genomic instability typical for cancer cells. Although the association between some mtDNA mutations and cancer is well established, the causative relationship between these two features is largely unknown. A hint suggesting the driving role of mtDNA mutations in carcinogenesis comes from the observation of tumor promotion after mtDNA depletion. Mitochondria with damaged DNA may alter signaling of the mitochondrial apoptosis pathway promoting cancer cell survival and conferring resistance to anticancer drugs. This resistance may be underlined by mtDNA copy number depletion. Therefore, mitochondria are considered a promising target in anticancer therapy and several mitochondria-targeting drugs are in preclinical and clinical trials. Some other aspects of mitochondrial structure and functions, including morphology and redox potential, can also be associated with cancer transformation and constitute new anticancer targets. Recently, several studies have disclosed new mechanisms underlying the association between mitochondria and cancer, including the protection of mtDNA by telomerase, suggesting new approaches in mitochondria-oriented anti-cancer therapy.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 4; 671-678
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic diversity of native Japanese dog breeds in the primitive type
    Różnorodność genetyczna rodzimych ras psów japońskich w typie pierwotnym
    Autorzy:
    Kloch, M.
    Życzyński, A.
    Nowak-Życzyńska, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/2082389.pdf
    Data publikacji:
    2021
    Wydawca:
    Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
    Tematy:
    Canis lupus familiaris
    mtDNA
    microsatellites
    control region
    primitive breeds
    Opis:
    Genetic diversity of native Japanese dog breeds in the primitive type. In the following paper the genetic distance among selected Japanese dog breeds (Akita, Kishu, Kai, Shiba, Shikoku, Hokkaido) was estimated. In order to determine genetic differentiation we analyzed a fragment of the mitochondrial control region and 10 microsatellite loci. We found that variation of the nuclear DNA was larger than that at the level of mitochondrial DNA. Within a fragment of 614 bp of control region, 13 haplotypes were identifi ed. The highest diversity of mitochondrial DNA was found in Akita. Based on mitochondrial DNA analysis, genetic distance between breeds ranged from 0.003 to 0.018. The lowest genetic distance was observed between Shikoku and Kai (0.003), and the highest between Shiba and Akita (0.018). After the analysis of nuclear DNA, 65 alleles were identifi ed. The mean percentage of polymorphic alleles in the applied microsatellite markers was 94.4% ±2.5%. The analysis showed that, the highest distance estimated on the frequency of microsatellite markers was found between Kai and Hokkaido (1.066), and the lowest between Shiba and Kai (0.212). Hokkaido and Shikoku were the only clearly distinguishing breeds.
    Źródło:
    Annals of Warsaw University of Life Sciences - SGGW. Animal Science; 2019, 58[1]; 37-45
    1898-8830
    Pojawia się w:
    Annals of Warsaw University of Life Sciences - SGGW. Animal Science
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mitochondrial DNA variability in Gyimesi Racka and Turcana sheep breeds
    Autorzy:
    Kusza, Szilvia
    Zakar, Erika
    Budai, Csilla
    Cziszter, Ludovic-Toma
    Padeanu, Ioan
    Gavojdian, Dinu
    Powiązania:
    https://bibliotekanauki.pl/articles/1039104.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    farm animal conservation
    genetic diversity
    mtDNA
    trans-boundary breeds
    Zackel group
    Opis:
    The current knowledge and documentation on the origins and relationship between Gyimesi Racka reared in Hungary and the Romanian Turcana is rather controversial. Lack of information and scientific reliable proofs for the divergent theories found in the two countries motivated us to implement a trial using molecular methods to assess the genetic distance and diversity in the two breeds. Hair follicles were collected from Gyimesi Racka (2 phenotypes) and from Turcana (6 ecotypes). The 599 bp segment of the D-loop region of the mitochondrial DNA was sequenced. Altogether, 42 haplotypes were identified, while 23 were found in both populations. Populations were highly diverse according to the haplotype and nucleotide diversity indices. AMOVA analysis showed that most of the variation was observed within populations (98%), indicating a weak genetic structure between the two breeds. Animals were grouped into seven groups based on their phenotype; however genetic distances among them were also low. Tajima's D, Fu's Fs, goodness-of-fit statistics, mismatch distribution and network analysis suggested recent demographic expansion. Current comprehensive mtDNA study indicates that there is very low level of genetic differentiation between the Gyimesi Racka and Turcana populations therefore they are de facto one trans-boundary breed.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 2; 273-280
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Deroceras panormitanum and congeners from Malta and Sicily, with a redescription of the widespread pest slug as Deroceras invadens n. sp.
    Autorzy:
    Reise, H.
    Hutchinson, J.M.C.
    Schunack, S.
    Schlitt, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/83241.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Mikołaja Kopernika. Wydział Biologii i Ochrony Środowiska. Stowarzyszenie Malakologów Polskich
    Tematy:
    Deroceras panormitanum
    Malta
    Sicily
    pest
    slug
    Deroceras invadens
    new species
    terrestrial slug
    taxonomy
    anatomy
    simultaneous hermaphrodite
    unilateral copulation
    genital morphology
    mating behaviour
    mtDNA
    cryptic species
    reproductive isolation
    Agriolimacidae
    Mediterranean region
    COI mtDNA sequencing
    Opis:
    The name Deroceras panormitanum is generally applied to a terrestrial slug that has spread worldwide and can be a pest; earlier this tramp species had been called Deroceras caruanae. Neither name is appropriate. The taxonomic descriptions apply to a species from Sicily and Malta. This true D. panormitanum and the tramp species are distinct in morphology and mating behaviour. For instance, the penial caecum of D. panormitanum is more pointed, everting faster at copulation. The size of the penial lobe varies considerably In preserved specimens but is always prominent at copulation. D. panormitanum is distinct from the Maltese endemic Deroceras golcheri, but a phylogeny based on mtDNA COI sequences implies that they are more closely related than is the tramp species. D. golcheri has a still closer counterpart on Sicily, but we leave the taxonomy of this “species X” unresolved. In interspecific crosses, D. panormitanum may transfer sperm to the partner’s sarcobelum whereas the partner fails to evert its penis (D. golcheri) or to transfer sperm (the tramp species). Names previously applied to the tramp species originally referred to D. panormitanum or are otherwise invalid, so it is here formally redescribed as D. invadens. Deroceras giustianum Wiktor, 1998 is synonymised with D. panormitanum.
    Źródło:
    Folia Malacologica; 2011, 19, 4
    1506-7629
    Pojawia się w:
    Folia Malacologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular characterization of nuclear and cytoplasmic genomes of Solanum × michoacanum (+) S. tuberosum somatic hybrids
    Autorzy:
    Smyda-Dajmund, P.
    Sliwka, J.
    Jakuczun, H.
    Wasilewicz-Flis, I.
    Zimnoch-Guzowska, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/951216.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    molecular characteristics
    nuclear genome
    Solanum tuberosum
    somatic hybrid
    DArT marker
    Solanum × michoacanum
    mtDNA
    cpDNA
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Evaluation of the suitability of mitochondrial DNA for species identification of microtraces and forensic traces
    Autorzy:
    Natonek-Wiśniewska, Małgorzata
    Krzyścin, Piotr
    Powiązania:
    https://bibliotekanauki.pl/articles/1038564.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    biological traces
    forensic DNA analysis
    species identification of forensic DNA
    species identification of biological traces
    mtDNA
    Opis:
    The objective of the study was to demonstrate how mitochondrial DNA (mtDNA) can be used to determine the species origin of animal microtraces. The study included pieces of cat and dog hair without the root, a fragment of cooked chicken bone (0.1g), three goose down samples (0.028 g), a pork swab, a pork scratching (5×5×5 mm), and pork lard (0.22 g). DNA was isolated from all of these samples using the method appropriate for the particular source material. The extracts had DNA concentration exceeding 5.4 ng/µl with A260/280 purity range of 1.14-1.88. Next, the samples were subjected to PCR and real-time PCR with species-specific primers and primers complementary to mitochondrial DNA (mtDNA). Control reactions based on the amplification of eukaryotic-specific fragment (18S rRNA) were additionally performed. PCR and real-time PCR products for detection of species-specific mtDNA were obtained for all templates, whereas during the detection of eukaryote DNA no product was obtained for dog and cat hair only. The poor quality of the obtained DNA did not prevent the analysis. The results showed that mitochondrial DNA is suitable for identification of small or highly processed samples, in which genomic DNA often cannot be analyzed.
    Źródło:
    Acta Biochimica Polonica; 2017, 64, 4; 705-708
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The genus Daphniola Radoman, 1973 (Caenogastropoda: Hydrobiidae) in the Peloponnese, Greece
    Autorzy:
    Falniowski, A.
    Szarowska, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/83994.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Mikołaja Kopernika. Wydział Biologii i Ochrony Środowiska. Stowarzyszenie Malakologów Polskich
    Tematy:
    Daphniola
    Caenogastropoda
    Hydrobiidae
    Peloponnese
    Greece
    valvatiform
    gastropod
    mtDNA
    anatomy
    hydrobiid
    protoconch
    phylogenetic analysis
    Daphniola hadei
    reproductive organ
    Opis:
    A valvatiform hydrobiid gastropod, found in a spring at Dhiaselo, W of Sparta, N. Taigetos Mts., Peloponnese, Greece, was identified as Horatia hadei Gittenberger, 1982. Its protoconch sculpture, female reproductive organs and penis morphology are characteristic of Daphniola Radoman, 1973. Maximum likelihood phylogenetic analysis based on COI (cytochrome oxidase subunit I) fragments of mtDNA proved that the species is congeneric with D. exigua (A. Schmidt, 1856) and D. louisi Falniowski et Szarowska, 2000, and thus belongs to the genus Daphniola, and that D. hadei, D. exigua and D. louisi are species-level distinct taxa.
    Źródło:
    Folia Malacologica; 2011, 19, 3
    1506-7629
    Pojawia się w:
    Folia Malacologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Species-specific chloroplast DNA polymorphism in the trnV-rbcL region in Pinus sylvestris and P. mugo
    Autorzy:
    Wachowiak, W
    Baczkiewicz, A.
    Celinski, K.
    Prus-Glowacki, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/41357.pdf
    Data publikacji:
    2004
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    Scotch pine
    Pinus sylvestris
    Pinus mugo
    dwarf pine
    hybridization
    DNA marker
    mtDNA
    trnV-rbcL region
    chloroplast
    DNA polymorphism
    Opis:
    Four cpDNA regions were analyzed with the use of PCR-RFLP technique and nucleotide sequences of two mtDNA regions were characterized in order to find P. sylvestris and P. mugo species specific markers useful for studies of the species hybridization. The difference in the restriction fragment patterns of trnV-rbcL region after digestion with MvaI endonuclease was detected. The analyses of the species representatives from various geographic regions revealed that the observed polymorphism is species specific. No differences have been disclosed in the analyzed trnS-trnT, trnK1-trnK2, trnC-trnD cpDNA regions. The P. sylvestris and P.mugo mtDNA sequences of orf25 and coxI regions proved to be identical.
    Źródło:
    Dendrobiology; 2004, 51; 67-72
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zastosowanie metod molekularnych w identyfikacji gatunku, wieku i płci owadów użytecznych w entomologii sądowej
    Use of molecular methods in the identification of the species, age and sex of insects useful in forensic entomology
    Autorzy:
    Stojak, Joanna
    Powiązania:
    https://bibliotekanauki.pl/articles/1373968.pdf
    Data publikacji:
    2014
    Wydawca:
    Centralne Laboratorium Kryminalistyczne Policji
    Tematy:
    entomologia sądowa
    barkoding DNA
    mtDNA
    metody molekularne
    identyfikacja gatunkowa
    forensic entomology
    DNA barcoding
    molecular methods
    identification of species
    Opis:
    Entomologia sądowa wykorzystuje owady do ustalania czasu i przyczyny śmierci, a nawet miejsca, w którym nastąpiła. W tym celu stosowane są dwie metody. Metoda rozwojowa opiera się na wzorcach rozwoju larw w określonych warunkach temperaturowo-środowiskowych. Metoda sukcesyjna analizuje występujące w różnych środowiskach wzorce pojawiania się poszczególnych taksonów na zwłokach. W obu tych metodach najistotniejszą kwestią jest poprawna identyfikacja gatunków. W poniższym artykule zaprezentowane zostały molekularne metody identyfikacji, takie jak barkoding DNA czy analiza krzywych denaturacji DNA o wysokiej rozdzielczości (DNA-HRM-PCR).
    Forensic entomology uses insects to determine the time, cause and place of death. To this end, two entomological methods are used. The development-based method uses the patterns of insect larvae development under the specific thermal and environmental conditions. The succession-based method analyzes the sequence of insect succession on the body in various environmental conditions. The proper insect species identification is essential in both methods. In this article, the molecular methods of species, age and sex identification are presented such as DNA barcoding or DNA-HRM-PCR.
    Źródło:
    Problemy Kryminalistyki; 2014, 286; 22-26
    0552-2153
    Pojawia się w:
    Problemy Kryminalistyki
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Phylogenetic relationships of Alzoniella slovenica (Lozek et Brtek, 1964) (Caenogastropoda: Hydrobiidae)
    Autorzy:
    Szarowska, M.
    Falniowski, A.
    Steffek, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/83587.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Mikołaja Kopernika. Wydział Biologii i Ochrony Środowiska. Stowarzyszenie Malakologów Polskich
    Tematy:
    cytochrome oxidase subunit 1
    mtDNA
    phylogenetic relationship
    Alzoniella slovenica
    Caenogastropoda
    Hydrobiidae
    Rissooidea
    morphology
    phylogenesis
    shell
    radula
    penis
    reproductive organ
    female
    snail
    Źródło:
    Folia Malacologica; 2011, 19, 2
    1506-7629
    Pojawia się w:
    Folia Malacologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Frequent D-loop polymorphism in mtDNA enables genotyping of 1400-year-old human remains from Merowingian graves
    Autorzy:
    Zeller, M
    Mirghomizadeh, F.
    Wehner, H.D.
    Blin, N.
    Powiązania:
    https://bibliotekanauki.pl/articles/2042042.pdf
    Data publikacji:
    2000
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    X chromosome
    ancient remains
    amplification technique
    Y chromosome
    ancient DNA
    mtDNA
    remains
    Merowingian culture
    polymorphism
    mitochondrial DNA
    man
    DNA marker
    DNA extraction
    DNA
    Źródło:
    Journal of Applied Genetics; 2000, 41, 4; 285-292
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Utility of two mitochondrial markers for identification of Picea abies refugial origin
    Autorzy:
    Litkowiec, M
    Dering, M.
    Lewandowski, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/41333.pdf
    Data publikacji:
    2009
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    coniferous plant
    tree
    Norway spruce
    Picea abies
    mtDNA
    molecular marker
    mitochondrial marker
    identification
    polymerase chain reaction
    RFLP analysis
    gene pool conservation
    forest ecosystem
    plant population
    Opis:
    Picea abies (L.) Karst is one of the most important coniferous species of Europe from both ecological and economical points of view. Traditional methods for the gene pool conservation and biodiversity maintenance in forest ecosystems have been practiced in many countries. For progress in this field using highly polymorphic genetic molecular markers is needed. Our goal was to demonstrate the utility of two polymorphic mitochondrial markers mt15-D02 and nad1 b/c in identification native Norway spruce stands. This molecular markers were tested in 1401 individuals from 59 Polish Norway spruce populations. We detected three alleles, which are called1, 2 and3, for locus mt15-D02 and two alleles , which are called1 and2, for locus nad1 b/c in our material. All five variants of alleles indicate the natural origin of P. abies. Result of this study shows that molecular marker mt15-D02 is easy to use and more informative in compare to marker nad1 b/c.
    Źródło:
    Dendrobiology; 2009, 61; 65-71
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Stan zdrowotny a zróżnicowanie genetyczne wybranych drzewostanów świerkowych na terenie RDLP w Krośnie
    Genetic variability and health of Norway spruce stands in the Regional Directorate of the State Forests in Krosno
    Autorzy:
    Gutkowska, J.
    Borys, M.
    Tereba, A.
    Tkaczyk, M.
    Oszako, T.
    Nowakowska, J.A.
    Powiązania:
    https://bibliotekanauki.pl/articles/1293265.pdf
    Data publikacji:
    2017
    Wydawca:
    Instytut Badawczy Leśnictwa
    Tematy:
    lesnictwo
    RDLP Krosno
    drzewostany swierkowe
    stan zdrowotny lasu
    drzewa lesne
    swierk pospolity
    Picea abies
    zroznicowanie genetyczne
    markery molekularne
    markery mikrosatelitarne
    DNA mitochondrialny
    Norway spruce
    health state
    SSR markers
    mtDNA
    genetic diversity
    Opis:
    The study was conducted in 2015 in six spruce stands situated in different forest districts administratively belonging to the Regional Directorate of Forests State in Krosno. Each spruce population was represented by 30 trees and assessed in terms of their current health status. Genetic analyses were performed based on shoot samples from each tree using nine nuclear DNA markers and one mitochondrial DNA marker (nad1). The health status of the trees was described according to the classification developed by Szczepkowski and Tarasiuk (2005) and the correlation between health classes and the level of genetic variability was computed with STATISTICA (α = 0.05). Nuclear DNA analyses revealed a low level of genetic variability among spruce populations (only 3% of the total genetic variation (FST = 0.028) and a high variability within populations (97%). The total heterozygosity in all stands (HT) was calculated as 0.646. Based on UPGMA analysis, the most genetically similar populations are spruce stands in the Bieszczady National Park and the Ustrzyki Dolne Forest District, which have the smallest genetic divergence of all populations (DN = 0.0165). Our analysis of the mitochondrial gene nad1 revealed the presence of six different haplotypes “a”, “a1”, “b”, “c”, “d” and “d1”. Comprising 56% of all haplotypes, “a” was the most common showing a predominant impact on spruce migration from the Carpathian area. The analysis based on mitochondrial markers (by Nei) revealed a heterozygosity of 0.525. Based on the observations of disease symptoms, 29% of the trees belong to health class 1,30% to class 2,28% to class 3 and class 4 contains 13% of trees. The comparison between health status and the level of genetic variation in the analyzed stands showed a positive correlation. Spruce stands with better health were also characterized by a greater degree of genetic variability. Since most of the investigated spruce populations shared the mitochondrial haplotype “a”, we have ascertained their Hercynian- Carpathian origin. Only one stand (Cisna) had a high frequency (43.3%) of the Nordic haplotype “c” suggesting that this provenance is derived from the Baltic post-glacial refugium of P. abies in Europe.
    Źródło:
    Leśne Prace Badawcze; 2017, 78, 1
    1732-9442
    2082-8926
    Pojawia się w:
    Leśne Prace Badawcze
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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