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Wyszukujesz frazę "Kohlmann, K." wg kryterium: Autor


Wyświetlanie 1-2 z 2
Tytuł:
Tracing the genetic origin of brown trout (Salmo trutta) re-colonizing the Ecker reservoir in the Hartz National Park, Germany
Autorzy:
Kohlmann, K.
Wüstemann, O.
Powiązania:
https://bibliotekanauki.pl/articles/363212.pdf
Data publikacji:
2012
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
pstrąg potokowy
Salmo trutta
mikrosatelitarne DNA
DNA
PCR
brown trout
microsatellite
Opis:
The Ecker reservoir and its main tributary had been free of brown trout (Salmo trutta) for several decades due to cumulative effects of natural and anthropogenic acidification. However, after the decline of emissions in the 1990s and the resulting rise of water pH to suitable for brown trout values, the species began to recolonize its original habitats. In the main tributary first brown trout individuals were caught in 2008 and in the reservoir in later years as well. Stocking could be excluded in both areas. Therefore, the present study was aimed to trace the genetic origin of these brown trout by genotyping eight microsatellite loci in samples collected in the reservoir, its main tributary, potential refugia and - for comparison - from two areas downstream of the dam being physically isolated for about 70 years. Genetic variability within populations (average number of alleles per locus), genetic differentiation between populations (FST values and genetic distances), occurrence of certain alleles and results of assignment tests indicated that the Ecker reservoir was re-colonized from two sources: the Große Peseke, a small direct inflow into the reservoir, and the Fuhler Lohnsbach, a parallel flowing brook connected to the reservoir by a pipe. Genetic data also supported recolonization of the main tributary from the reservoir but not in the opposite direction. Moreover, bottleneck effects were evident in brown trout populations upstream of the dam compared to the two populations downstream of the dam.
Źródło:
Environmental Biotechnology; 2012, 8, 2; 39-44
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
New microsatellite multiplex PCR sets for genetic studies of the sterlet sturgeon, Acipenser ruthenus
Autorzy:
Kohlmann, K.
Kersten, P.
Geßner, J.
Onără, D.
Taflan, E.
Suciu, R.
Powiązania:
https://bibliotekanauki.pl/articles/363202.pdf
Data publikacji:
2017
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
Acipenser ruthenus
microsatellite
multiplex PCR
species conservation
sustainable fisheries
Opis:
Wild populations of the sterlet sturgeon, Acipenser ruthenus, are declining throughout their native ranges. In-depth knowledge of their genetic diversity and structure is urgently needed to enable the identification of management units for conservation purposes. Moreover, genetic markers are required to establish appropriate breeding schemes for supportive stocking programs and to monitor genetic changes in farmed stocks. Therefore, six species-specific, polymorphic microsatellite loci were isolated and arranged into five multiplex PCR sets together with nine loci from other sturgeon species. The diversity of these 15 microsatellites was examined in 67 sterlet individuals (20 farmed in Germany and 47 wild-caught in the Romanian part of the River Danube). The total number of alleles per locus ranged from 3 to 15 with an average of 7.20. The farmed sterlet sturgeon possessed 1 to 7 alleles per locus, with a mean of 3.13; the wild individuals were more variable, with 3 to 15 alleles per locus and a mean of 7.07. Observed heterozygosities ranged from 0 to 0.850 in the farmed individuals, and from 0.064 to 0.957 in the wild individuals. Indications of inbreeding were only found in the wild sterlet sturgeon (FIS=0.062). The genetic differentiation of the two sterlet groups was significant (FST=0.1186). The high sensitivity and discriminatory power of the 15 loci was indicated by the very low overall probability of identity for siblings (PIsib=5.099x10-5) and the high accuracy of self-classification (66 out of the 67 individuals (98.51%) were correctly identified). Thus, these newly developed multiplex PCR sets are a valuable genetic tool for identifying management units for species conservation, sustainable fisheries and aquaculture.
Źródło:
Environmental Biotechnology; 2017, 13, 1; 11-17
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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