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    Wyświetlanie 1-9 z 9
    Tytuł:
    Liquid shoot culture of Salvia officinalis L. for micropropagation and production of antioxidant compounds; Effect of triacontanol
    Autorzy:
    Grzegorczyk, I
    Wysokinska, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/58539.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    Salvia officinalis
    medicinal plant
    triacontanol
    liquid shoot culture
    botany
    herb
    micropropagation
    production
    antioxidant compound
    sage
    Opis:
    Liquid shoot culture of Salvia officinalis L. in MS medium containing IAA (0.1 mg l-1) and BAP (0.45 mg l-1) was developed and evaluated in relation to shoot multiplication and antioxidant compound (carnosic acid, carnosol and rosmarinic acid) accumulation. In the liquid medium, on average, 3 new shoots per explant (shoot tip) were obtained within 3 weeks. The shoots produced 8.2±0.02 mg of diterpenoids and 31.2±0.29 mg of rosmarinic acid per gram of dry weight. Shoot proliferation and diterpenoid content increased when triacontanol (5, 10 or 20 pg l-1) was added to the liquid medium. In optimum conditions (at 20 pg l-1 TRIA) almost 7 shoots were formed per explant after 3 weeks. An increase in diterpenoid production (expressed as the sum of carnosol and carnosic acid) ranged from 30% to 50% and dependended on triacontanol concentration tested. The level of diterpenoids in triacontanol-treated shoots was similar to the content of compounds in commercial herbal product (dried leaves of S. officinalis) (10-12 mg g-1 dry wt). Triacontanol did not increase rosmarinic acid production, but the content of the phenolic as compound in shoots grown in liquid culture (31 mg g-1 dry wt) was even 24 times higher compared to samples of dried leaves of S. officinalis plants. We also demonstrated that the highest amounts of CA, Car and RA were accumulated in young, top parts of sage shoots. This observation could be useful for improving the selection of material for the extraction of natural antioxidants from S. officinalis.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2008, 77, 2
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Development of highbush blueberry (Vaccinium corymbosum hort. non L.) in vitro shoot cultures under the influence of melatonin
    Rozwój pędowych kultur in vitro borówki wysokiej (Vaccinium corymbosum hort. non L.) pod wpływem melatoniny
    Autorzy:
    Litwińczuk, W.
    Wadas-Boroń, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/11541782.pdf
    Data publikacji:
    2009
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    Northern highbush blueberry
    Vaccinium corymbosum
    in vitro culture
    melatonin
    plant development
    shoot culture
    auxin
    micropropagation
    Herbert cultivar
    Opis:
    The influence of melatonin (MEL) in comparison with IAA, IBA (all at 5.71 µ M), and auxin-free (0) medium on development of highbush blueberry (Vaccinium corymbosum hort. non L.) ‘Herbert’ in vitro shoot cultures was examined. Depending on the kind of hormone in vitro cultures consisted of various number of axillary (AX) and adventitious (AD) shoots. The influence of melatonin on in vitro cultures was intermediate but more similar to IAA than IBA. Production of axillary shoots on media supplemented with MEL and IAA was comparable and higher than on medium with IBA. In contrast to IAA melatonin reduced development of adventitious shoots. Contrary to IBA-obtained cultures AX shoots grown on ‘IAA’, ‘0’, and ‘MEL’ media resembled AD shoots.
    W pracy badano wpływ melatoniny (MEL 5.71 µ M) na rozwój pędowych kultur in vitro borówki wysokiej (Vaccinium corymbosum hort.  non L. ‘Herbert’). Odniesienie stanowiły pożywki zawierające IAA, IBA (5.71 µ M) lub wolne (0) od auksyn. W zależności od rodzaju hormonu kultury in vitro składały się z różnej liczby pędów kątowych (AX) i przybyszowych (AD). Wpływ melatoniny na kultury in vitro był pośredni, choć bardziej podobny do wpływu IAA niż IBA. Wytwarzanie pędów kątowych na pożywkach z dodatkiem melatoniny i IAA było zbliżone i większe niż na pożywce z IBA. W przeciwieństwie do IAA melatonina osłabiała rozwój pędów przybyszowych. Pędy kątowe otrzymane przy użyciu IBA wyraźnie różniły się od pędów przybyszowych. Na pozostałych pożywkach (‘IAA’, ‘0’ i ‘MEL’) wygląd pędów kątowych i przybyszowych był podobny.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2009, 08, 3; 3-12
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Micropropagation of Grewia tenax (Forssk.) Fiori – an important ethnomedicinal plant
    Autorzy:
    Daffalla, H.M.
    Elsheikh, A.M.
    Ali, H.A.
    Khalafalla, M.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/80945.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    ethnomedicine
    Grewia tenax
    micropropagation
    shrub species
    natural environment
    seed dormancy
    cotyledon
    benzyladenine
    kinetin
    adenine
    thidiazuron
    multiple shoot culture
    shoot multiplication
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2019, 100, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Micropropagation of Plantago asiatica L. through culture of shoot-tips
    Autorzy:
    Makowczynska, J
    Andrzejewska-Golec, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/58965.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    shoot tip culture
    rooting
    micropropagation
    medicinal plant
    Plantago asiatica
    Plantaginaceae
    in vitro
    medicinal species
    Opis:
    Shoot-tip multiplication of the medicinal species - Plantago asiatica was carried on MS medium with IAA and BAP or kinetin. Best results in micropropagation were achieved by adding 0.1 mg/dm3 IAA and 1 mg/dm3 BAP. After 6 weeks shoots were transferred to MS medium for rooting. The resulting plantlets were transferred after 8 weeks into pots and after a period of adaptation into the ground (field culture). The species Plantago asiatica was propagated in vitro by shoot-tip multiplication for the first time.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2003, 72, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants
    Autorzy:
    Li, J.
    Kang, Y.
    Qiang, S.
    Peng, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/56457.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    propagation
    goldenrod
    Solidago canadensis
    leaf
    nodal explant
    callus formation
    micropropagation
    adventitious shoot
    tissue culture
    Opis:
    Goldenrod (Solidago canadensis L.) is an invasive plant species in many countries except North America but a cut-flower species worldwide. There is a need to generate and propagate goldenrod clones efficiently for research and commercial purposes. A callus induction and plantlet regeneration system was developed by studying the influence of explant type and different concentrations of plant growth regulators. The highest callus production from leaf segments was obtained on Murashige and Skoog's medium (MS medium) supplemented with 1.0 mg/L naphthalene acetic acid (NAA) and 1.0 mg/L 6-benzylaminopurine (BA). Adventitious shoots could be regenerated directly from leaf explants without an intermediate callus phase with the highest shoot induction percentage of 87.2%. The largest number of adventitious shoots per leaf explant (3.2) was obtained on MS medium supplemented with 0.4 mg/L NAA and 2.0 mg/L BA. MS medium supplemented with 0.1 mg/L NAA and 1.0 mg/L BA was the best medium for axillary shoot regeneration from nodal segments. The highest root number and longest roots occurred on half-strength MS without the addition of any growth regulator. Rooted plantlets were then transferred to a soil-based growth medium, placed in a greenhouse, and acclimatized with 100% success. All surviving plants grew normally without showing any morphological variation when compared to those grow from seed. This regeneration protocol may be used to produce certain biotypes of goldenrod suitable for genetic transformation, rapid propagation of goldenrod for commercial purposes or for screening fungi and toxins as potential biocontrol agents against this weed.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2012, 81, 1
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro callus and shoot organogenesis from leaf and stem explants of Chamaedaphne calyculata
    Autorzy:
    Zrobek-Sokolnik, A.
    Dynowski, P.
    Holdynski, C.
    Powiązania:
    https://bibliotekanauki.pl/articles/41437.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    in vitro culture
    callus
    shoot
    organogenesis
    leaf
    stem
    explant
    Chamaedaphne calyculata
    Ericaceae
    micropropagation
    plant species
    plant conservation
    Opis:
    Leatherleaf Chamaedaphne calyculata (L) Moench is a relict, rare and endangered species in Poland. There are no reports on the micropropagation of Chamaedaphne calyculata in the literature. Therefore, the aim of this study was to propose a propagation protocol for leatherleaf via indirect organogenesis using leaves and stems (internodal segments) derived from mature plants growing in a natural stand and from plants grown in vitro as explants. The medium developed by Anthony et al. (2004) with 100%, 50% and 25% salt concentrations, supplemented with IAA (5 and 10μM) and TDZ (5 and 10μM), was used for callus development and the induction of adventitious shoots. The media developed by Anthony et al. (2004) and Anderson (1980), both containing 10μM TDZ and 5μM IAA or 2.28μM zeatin, were used for adventitious shoot elongation. Secondary explants proved to be the most effective starting material for callus induction, the regeneration and elongation of adventitious shoots. The most supportive medium for callus induction and growth and the induction of adventitious shoots was the full medium proposed by Anthony et al. (2004) containing 5μM IAA and 10μM TDZ. Anderson’s (1980) medium containing 2.28μM zeatin delivered optimal results in the elongation of adventitious shoots of Ch. calyculata. Roots were cultivated on Anderson’s (1980) phytohormone-free medium. Approximately 65% of the plantlets survived after transfer to the sphagnum-peat and perlite mixture (3:1). The plants grew normally without any signs of morphological variation. This study makes the first ever attempt to propose an effective micropropagation protocol for Ch. calyculata.
    Źródło:
    Dendrobiology; 2014, 72
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Withania coagulans (Stocks) Dunal: biotechnological achievements and perspectives
    Autorzy:
    Teixera da Silva, J.A.
    Kher, M.M.
    Soner, D.
    Nataraj, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/1928.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Ogrodnictwa
    Tematy:
    Withania coagulans
    medicinal plant
    Solanaceae
    shoot regeneration
    rooting
    in vitro
    micropropagation
    tissue culture
    random amplified polymorphic DNA
    Źródło:
    Journal of Horticultural Research; 2015, 23, 1
    2300-5009
    Pojawia się w:
    Journal of Horticultural Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The use of nano-silver for disinfection of Pennisetum alopecuroides plant material for tissue culture
    Autorzy:
    Parzymies, M.
    Pudelska, K.
    Poniewozik, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/12683367.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    Pennisetum alopecuroides
    grass species
    ornamental grass
    shoot
    explant
    plant material
    tissue culture
    plant growth regulator
    micropropagation
    contamination
    plant regeneration
    silver
    nanoparticle
    disinfection
    Opis:
    Initiation of tissue culture of many plant species is a very difficult stage due to appearance of many contaminations. The other problem might be a choice of media for regeneration. Initiation of grass species tissue cultures are thought to be very difficult. Therefore, a research was undertaken to evaluate the use of nano-silver particles for plant material disinfection and to estimate a medium Pennisetum alopecuroides. The plant material were buds and nodal explants that were disinfected in 2% NaOCl for 30 min or 0.1% HgCl2 for 1 min. Half of the explants disinfected with NaOCl were soaked in 50, 100 or 250 mg·dm–3 Ag NPs for 1 hour. Explants not soaked in nano-silver were placed on media with Ag NPs at concentrations of 4, 8 or 16 mg·dm–3. An influence of growth regulators on Pennisetum alopecuroides was evaluated in vitro. Regenerated shoots were placed on MS media with: 3 mg·dm–3 BA + 0.3 mg·dm–3 IBA, 3 mg·dm–3 KIN + + 0.3 mg·dm–3 IAA, 1 mg·dm–3 BA + 0.1 mg·dm–3 IBA. It was observed that the use of nano-silver particles lowered the level of contamination. The best results were obtained when Ag NPs was used at concentration of 100–250 mg·dm–3 alone or as a supplementation of the media, at concentration of 4 mg·dm–3 for nodes and 16 mg·dm–3 for adventitious buds. The use of nodal explants allowed to obtain less contamination. Regeneration depended on a media content. The most regenerated shoots were obtained on the MS media supplemented with 1 mg·dm–3 BA and 0.1 mg·dm–3 IBA.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 3; 127-135
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Micropropagation of Stryphnolobium japonicum
    Autorzy:
    Kobus, M
    Kulpa, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/41239.pdf
    Data publikacji:
    2009
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    Styphnolobium japonicum zob.Sophora japonica
    Sophora japonica
    Japanese pagoda tree
    pagoda tree zob.Japanese pagoda tree
    scholar zob.japanese pagoda tree
    Chinese scholar zob.Japanese pagoda tree
    micropropagation
    vegetation
    development
    in vitro culture
    old tree
    growth regulator
    primary regeneration
    shoot multiplication
    rooting
    Opis:
    Observations of Japanese pagoda trees indicate that they undergo a full cycle of vegetative and generative development without self-renovation. The aim of this study was to obtain a successive media protocol propagation of Japanese pagoda tree by in vitro cultures. The effects of growth regulators were studied with reference to primary regeneration, shoot multiplication and rooting. As explants source were used the part of shoot of 90-year-oldtree. Explants were placed on MS (Murashige and Skoog 1962) basal medium with the addition of 6-benzylaminopurine – BAP (1.0–2.0 mg dm-3), thidiazuron – TDZ (0.1–0.3 mg dm-3) and indole-3-acetic acid – IAA (0.5–1.0 mg dm-3). BAP was the growth regulator which significantly increased shoot regeneration on initial explants. TDZ in turn, inhibited the formation of adventitious shoots and caused the explants which had been placed on the medium to die. The multiplication of the Japanese pagoda tree by in vitro cultures should be conducted on MS media with the addition of 0.5 mg dm-3 BAP, and they should be rooted on media with the addition of 0.3 mg dm-3 indole-3-butyric acid – IBA. It seems, that devising an efficient method of Japanese pagoda tree micropropagation is realistic.
    Źródło:
    Dendrobiology; 2009, 61; 23-26
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-9 z 9

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