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    Wyświetlanie 1-12 z 12
    Tytuł:
    Cultivation of soapwort plants (Saponaria officinalis L.) in in vitro cultures
    Autorzy:
    Figas, Anna
    Lefelbajn, Weronika
    Powiązania:
    https://bibliotekanauki.pl/articles/16243498.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Stowarzyszenie Infrastruktura i Ekologia Terenów Wiejskich PAN
    Tematy:
    medicinal plant
    axillary shoots
    micropropagation
    plant growth regulator
    Opis:
    Saponaria officinalis L. is a medicinal plant from the family Caryophyllaceae. It occurs in most European countries, as well as in North Africa, America and Western Asia. The aim of the study was to find an optimal method of sterilization S. officinalis seeds in order to obtain sterile seedlings and to multiply axillary shoots from nodal explants isolated from them, on MS medium with the addition of growth regulators (RW). In this study, 4 variants of seed sterilization were compared using NaClO at the following concentrations: 1.5% (2), 2% (3) and 2.5% (4) for a period of 11 minutes. The control was a variant in which 70% C2H5OH was used for pre-sterilization for 1 minute (1). The highest percentage of sprouted, seeds and sterile seedlings and the lowest percentage of contamination were obtained in variant (4). As a result of micropropagation of soapwort in in vitro cultures, axillary shoots were formed from nodal explants from axillary buds. The highest percentage of explants with shoots (95%) and callus tissue (48%) and the highest number of shoots from one explant (5.95) were obtained in the second passage on MS medium with the addition of 4 mg.dm-3 BAP and 0.5 mg.dm-3 NAA. Due to the wide possibilities of using soapwort, it is advisable to continue research aimed at developing an optimal and efficient plant regeneration system of this species.
    Źródło:
    Infrastruktura i Ekologia Terenów Wiejskich; 2023, I/1; 59--69
    1732-5587
    Pojawia się w:
    Infrastruktura i Ekologia Terenów Wiejskich
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Micropropagation of Calycanthus fertilis
    Autorzy:
    Kulpa, D.
    Kubus, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/41353.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    in vitro culture
    micropropagation
    Calycanthus fertilis
    multiplication
    plant growth regulator
    Opis:
    Calycanthus fertilis Walt. is a shrub belonging to the family Calycanthaceae, has great potential as ornamental. In the literature there are no reports on methodpropagation of this shrub in in vitro cultures. Therefore, the aim of this study was a development of the method micropropagation of Calycanthus fertilis Walter. Shoot explants of the size 1 cm, with an apex or node with lateral meristems were placed on the media with mineral composition according to MS and WPM supplemented with BAP (from 0.5 to 2.0 mg·dm–3) and TDZ (from 0.1 to 0.5 mg·dm–3). BAP turnedout to significantly increase initiation frequency whereas TDZ inhibitedthe formation of adventitious shoots andcausedexplant death. The highest percentage of initiated explants were found in shoot fragments placed on WPM medium supplemented with 1.0 mg·dm–3 BAP. Primary explants which initiatedgrowth were transferredon the proliferation media containing WPM macroand microelement, with the addition of different cytokinin: BAP (from 0.5 to 2.5 mg·dm–3), KIN (1.0 to 5.0 mg·dm–3) or TDZ (from 0.1 to 0.5 mg·dm–3). Calycanthus multiplication in vitro shouldbe conductedon WPM media with 1.0 mg·dm–3 BAP. Proliferatedshoots were placedon the WPM rooting medium supplemented with auxins: IBA, IAA or NAA at the concentration from 0.1 to 2.0 mg·dm–3. Maximum rooting was obtained on WPM medium supplemented with 0.5 mg·dm–3 IBA. To sum up, it shouldbe statedthat an efficient method of micropropagation of Calycanthus fertilis Walt. has been developed.
    Źródło:
    Dendrobiology; 2010, 64
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Micropropagation studies and antioxidant analysis of the endangered plants of Bulgarian yellow gentian (Gentiana lutea L.)
    Autorzy:
    Petrova, M.I.
    Zayova, E.G.
    Dimitrova, L.I.
    Geneva, M.P.
    Miladinova-Georgieva, K.D.
    Powiązania:
    https://bibliotekanauki.pl/articles/12687267.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    yellow gentian
    Gentiana lutea
    herbal plant
    medicinal plant
    endangered plant
    micropropagation
    seed germination
    plant growth regulator
    antioxidant
    antioxidant analysis
    Opis:
    In order to develop an efficient micropropagation system, it is essential to establish the appropriate concentration of growth regulators for seed germination, shoot formation and rooting. Nodal segments from in vitro obtained seedlings of Gentiana lutea L. were cultured in vitro in Murashige and Skoog’s medium supplemented with BAP, Thidiazuron and Zeatin (0.5, 1.0 and 2.0 mg L−1). A maximum number of shoots with the highest height was recorded at 2.0 mg L−1 BAP. For further optimization of the process, we used nutrient media containing BAP and Zeatin with a combination of low concentration of Indoleacetic acid. MS medium containing 2.0 mg L−1 Zeatin and 0.2 mg L−1 IAA resulted in maximum numbers of shoots 94.3) with shoot height 2.5 cm. The multiple plants were successfully ex vitro acclimatized with 65% survival. The presence of growth regulators (2.0 mg L−1 Zeatin and 0.2 mg L−1 IAA) in the nutrient media resulted in an effective antioxidant activity in G. Lutea determined by the low molecular antioxidant metabolites such as phenols and flavonoids and activities of antioxidant enzymes – catalase, ascorbate peroxidase, guaiacol peroxidase, and superoxide dismutase. The described protocol allows the establishment of numerous micropropaged plants of rare and endangered G. lutea.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 3; 71-78
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A non caloric sweetener Stevia rebaudiana Bertoni - tissue cultured plantlets for organic farming and home gardening
    Autorzy:
    Tomaszewska-Sowa, M.
    Figas, A.
    Sawilska, A.K.
    Powiązania:
    https://bibliotekanauki.pl/articles/59692.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Stowarzyszenie Infrastruktura i Ekologia Terenów Wiejskich PAN
    Tematy:
    non-caloric sweetener
    sweetener
    Stevia rebaudiana
    micropropagation
    plant growth regulator
    tissue culture
    plantlet
    organic farming
    home garden
    Źródło:
    Infrastruktura i Ekologia Terenów Wiejskich; 2015, IV/3
    1732-5587
    Pojawia się w:
    Infrastruktura i Ekologia Terenów Wiejskich
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro flowering and micropropagation of Lisianthus (Eustoma grandiflorum) in response to plant growth regulators (NAA and BA)
    Kwitnienie i mikropropagacja in vitro lisianthusa (Eustoma grandiflorum) w reakcji na regulatory wzrostu roslin (NAA i BA)
    Autorzy:
    Kaviani, B.
    Zamiraee, F.
    Zanjani, S.B.
    Tarang, A.
    Torkashvand, A.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/11542950.pdf
    Data publikacji:
    2014
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    in vitro culture
    ornamental plant
    flowering
    micropropagation
    Lisianthus
    Eustoma grandiflorum
    plant response
    plant growth regulator
    NAA potassium salt
    axillary bud
    callus
    plant tissue culture
    Opis:
    In vitro flowering and micropropagation are useful for plant breeding programs and commercial production of important ornamental plants. In vitro conditions including media components, kind, concentration and ratio of plant growth regulators and culture conditions significantly affect in vitro flowering and micropropagation. There is no any report dealing with the in vitro flowering of Lisianthus (Eustoma grandiflorum). Here, a protocol was developed for flowering and high frequency in vitro micropropagation of E. grandiflorum, an ornamental plant. Micropropagation is an effective tools for propagation of ornamental plants in large scale. The aim of the present study was to evaluate the effect of different concentrations of NAA and BA on micropropagation and flowering of Lisianthus, in vitro. Used culture medium was MS enriched with 0, 0.1, 0.2 and 2 mg L-1 of NAA and BA. In establishment process of explants, the most shoot length (2.07 cm per plant) was obtained on medium supplemented with 0.1 mg L-1 BA (without NAA). Maximum shoot number (5.80 per plant) was produced in medium containing 0.1 mg L-1 BA along with 0.2 mg L-1 NAA. Bud explants in culture media containing 0.2 mg L-1 NAA (without BA) and 0.1 mg L-1 NAA along with 2 mg L-1 BA produced maximum node number (3.20 per plant). The largest number of root (14.53 per plant) and root length (3.87 cm per plant) were produced on 0.2 mg L-1 NAA without BA, also 0.2 mg L-1 BA plus 0.2 mg L-1 NAA and 0.2 mg L-1 BA without NAA. Explants produced flower on medium containing 0.1 mg L-1 BA along with 0.1 mg L-1 NAA without transition of callus formation. Flower was produced from callus in medium containing 0.1 mg L-1 BA along with 2 mg L-1 NAA. Regenerated plants showed 98% survival in greenhouse during acclimatization. Acclimatized plants were morphologically similar to the mother plants.
    Kwitnienie i mikropropagacja in vitro są użyteczne w programach hodowli roślin oraz produkcji komercyjnej ważnych roślin ozdobnych. Warunki in vitro, łącznie ze składnikami pożywek, rodzajem, stężeniem oraz proporcją regulatorów wzrostu roślin, a także warunkami hodowli, w sposób istotny wpływają na kwitnienie i mikropropagację in vitro. Nie istnieje żadne badanie dotyczące kwitnienia in vitro lisanthiusa (Eustoma grandiflorum). W niniejszym badaniu opracowano kwitnienie i wysoką częstotliwość mikropropagacji in vitro dla E. grandiflorum, który jest rośliną ozdobną. Mikropropagacja jest skutecznym narzędziem rozmnażania roślin ozdobnych na dużą skalę. Celem niniejszego badania była ocena wpływu różnych stężeĔ NAA i BA na mikropropagację i kwitnienie lisianthiusa in vitro. Używana pożywka hodowlana została wzbogacona za pomocą 0; 0,1; 0,2 i 2 mg L-1 NAA i BA. Przy powstawaniu eksplantów największa długość łodygi (2,07 cm na roślinę) była uzyskana na pożywce uzupełnionej o 0,1 mg L-1 BA (bez NAA). Maksymalna liczba łodyg (5,80 na roślinę) została wytworzona na pożywce zawierającej 0,1 mg L-1 BA wraz z 0,2 mg L-1 NAA. Eksplanty pączków na pożywce hodowlanej zawierającej 0,2 mg L-1 NAA (bez BA) oraz 0,1 mg L-1 NAA wraz z 2 mg L-1 BA wytworzyły maksymalną liczbę węzłów (3,20 na roślinę). Największą liczbę korzeni (14,53 na roślinę) oraz największą długość korzenia (3,87 na roślinę) zaobserwowano na 0,2 mg L-1 NAA bez BA jak również 0,2 mg L-1 BA plus 0,2 mg L-1 NAA oraz 0,2 mg L-1 BA bez NAA. Eksplanty tworzyły kwiat na pożywce zawierającej 0,1 mg L-1 BA wraz z 0,1 mg L-1 NAA bez przeniesienia kalusa. Kwiat był tworzony z kalusa na pożywce zawierającej 0,1 mg L-1 BA wraz z 2 mg L-1 NAA. Zregenerowane rośliny wykazały 98% przeżycie w szklarni podczas aklimatyzacji. Zaaklimatyzowane rośliny były morfologicznie podobne to swych roślin macierzystych.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2014, 13, 4; 145-155
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro multiplication and acclimatization of Biscutella laevigata (Brassicaceae) to cultivation in greenhouse conditions
    Autorzy:
    Hanus-Fajerska, E.
    Wiszniewska, A.
    Muszynska, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/80691.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Biscutella laevigata
    biostimulator
    in vitro
    propagation
    multiplication
    micropropagation
    cultivation
    greenhouse condition
    vegetative propagation
    photosynthetic photon flux density
    plant growth regulator
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2012, 93, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The use of nano-silver for disinfection of Pennisetum alopecuroides plant material for tissue culture
    Autorzy:
    Parzymies, M.
    Pudelska, K.
    Poniewozik, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/12683367.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    Pennisetum alopecuroides
    grass species
    ornamental grass
    shoot
    explant
    plant material
    tissue culture
    plant growth regulator
    micropropagation
    contamination
    plant regeneration
    silver
    nanoparticle
    disinfection
    Opis:
    Initiation of tissue culture of many plant species is a very difficult stage due to appearance of many contaminations. The other problem might be a choice of media for regeneration. Initiation of grass species tissue cultures are thought to be very difficult. Therefore, a research was undertaken to evaluate the use of nano-silver particles for plant material disinfection and to estimate a medium Pennisetum alopecuroides. The plant material were buds and nodal explants that were disinfected in 2% NaOCl for 30 min or 0.1% HgCl2 for 1 min. Half of the explants disinfected with NaOCl were soaked in 50, 100 or 250 mg·dm–3 Ag NPs for 1 hour. Explants not soaked in nano-silver were placed on media with Ag NPs at concentrations of 4, 8 or 16 mg·dm–3. An influence of growth regulators on Pennisetum alopecuroides was evaluated in vitro. Regenerated shoots were placed on MS media with: 3 mg·dm–3 BA + 0.3 mg·dm–3 IBA, 3 mg·dm–3 KIN + + 0.3 mg·dm–3 IAA, 1 mg·dm–3 BA + 0.1 mg·dm–3 IBA. It was observed that the use of nano-silver particles lowered the level of contamination. The best results were obtained when Ag NPs was used at concentration of 100–250 mg·dm–3 alone or as a supplementation of the media, at concentration of 4 mg·dm–3 for nodes and 16 mg·dm–3 for adventitious buds. The use of nodal explants allowed to obtain less contamination. Regeneration depended on a media content. The most regenerated shoots were obtained on the MS media supplemented with 1 mg·dm–3 BA and 0.1 mg·dm–3 IBA.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 3; 127-135
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro propagation of ‘Gisela 5’ rootstock as affected by mineral composition of media and plant growth regulators
    Autorzy:
    Fallahpour, M.
    Miri, S.H.
    Bouzari, N.
    Powiązania:
    https://bibliotekanauki.pl/articles/2041.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Ogrodnictwa
    Tematy:
    in vitro propagation
    rootstock
    micropropagation
    Prunus cerasus × Prunus canescens
    Gisela 5 cultivar
    proliferation
    rooting
    mineral composition
    plant growth regulator
    Źródło:
    Journal of Horticultural Research; 2015, 23, 1
    2300-5009
    Pojawia się w:
    Journal of Horticultural Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro proliferation and ex vitro rooting of microshoots of lisianthus using auxin and cytokinin on solid, liquid and double-phase culture systems
    Autorzy:
    Kaviani, B.
    Bahari, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/12678778.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    Gentianaceae
    Eustoma
    Lisianthus
    Eustoma grandiflorum
    ornamental plant
    flowering plant
    rooting
    microshoot
    micropropagation
    in vitro proliferation
    ex vitro method
    soild phase
    liquid phase
    double-phase culture
    plant growth regulator
    auxin
    cytokinin
    Opis:
    A protocol was developed for high frequency and low cost of in vitro shoot proliferation and ex vitro rooting of Eustoma grandiflorum (Gentianaceae) on solid medium. Shoot tips as explants were cultured on Murashige and Skoog (MS) medium enriched with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.00, 0.01, 0.10 and 1.00 mg l–1) and 6-benzylaminopurine (BAP) (0.00, 0.50, 2.00 and 5.00 mg l–1). Three culture media systems (solid, liquid and double-phase) were applied. None of the explants cultured on liquid and double-phase media resulted in live plant production. Maximum axillary shoot number (54.45) was recorded in the plantlets treated with 0.10 mg l–1 2,4-D in combination with 5.00 mg l–1 BAP. Treatment of 0.01 mg l–1 2,4-D along with 0.50 mg l–1 BAP produced maximum node number and internode length. Some shoots produced on medium containing plant growth regulators (PGRs) were rooted in soil. The largest number (5.50/plantlet) and longest length of root (7.75 cm/plantlet) were obtained in ex vitro condition on the base of shoots produced in culture medium enriched with 0.10 mg l–1 2,4-D along with 0.50 mg l–1 BAP. The combination of 1.00 mg l–1 2,4-D and 0.50 mg l–1 BAP was found to be the most suitable PGRs for obtaining the highest callus weight. The most fresh weight was calculated from plantlets grown on the medium containing 0.10 mg l–1 2,4-D along with 5.00 mg l–1 BAP. Maximum dry weight was obtained in free-PGRs medium. About 90% of the rooted plantlets were established successfully in cultivation beds. Acclimatized plants were morphologically similar to the mother plants.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 47-56
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Sustainable micropropagation of selected Stevia rebaudiana Bertoni genotypes
    Autorzy:
    Kaplan, B.
    Duraklioglu, S.
    Turgut, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/12691030.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    Turkey
    plant cultivation
    Compositae
    Stevia rebaudiana
    steviol glycoside
    sweetener
    micropropagation
    in vitro propagation
    growth regulator
    genotype
    Gelrite preparation
    Opis:
    Stevia rebaudiana Bertoni is a perennial plant belonging to Asteraceae family and its leaves contain steviol glycosides (SGs) that are 150 to 300 times sweeter than sucrose. The sweeteners obtained from S. rebaudiana can be safely used by diabetics as insulin secretion is not required during digestion of this sweetener. As it has zero calories, it is also used in diet products. Adaptation studies for Stevia conducted in Antalya, Turkey have shown that the stevia plant could easily be cultivated as a perennial. However, the lack of a sustainable vegetative propagation method creates a significant problem for stevia production. In the generatively populations, homogeneity and therefore quality are decreased because of cross-pollination. Stevia, as a self-incompatible and cross-pollinated species, has been shown to have very high genetic diversity. Therefore, development of a sustainable in vitro propagation method to prevent genetic heterogeneity of selected varieties is crucial for stevia cultivation. The aim of this study was to evaluate 2 different gelling agents (plant agar and Gelrite) and 20 different growth regulators combinations. The results demonstrated an approximately 200-fold multiplication rate obtained within 13 weeks using MS medium supplemented with 0.5 mg·dm–3 BAP and 0.25 mg·dm–3 kinetin and solidified with Gelrite. Average stevioside and rebaudioside A contents in in vitro propagated plant samples were found to be 8.1% and 8.6%, respectively.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 6; 47-56
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of growth regulators on dahlia propagation in tissue culture and acclimatization of plants in ex vitro conditions
    Autorzy:
    Marcinek, B.
    Parzymies, M.
    Poniewozik, M.
    Kozak, D.
    Durlak, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/12307379.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    Dahlia
    Dahlia x cultorum
    dahlia
    ornamental plant
    flowering plant
    tuberous plant
    tuberous root
    plant acclimatization
    growth regulator
    cytokinin
    gibberellic acid
    micropropagation
    tissue culture
    ex vitro method
    microcutting
    Opis:
    The aim of the work was to evaluate the influence of cytokinins on in vitro propagated dahlia and their consequent effect on acclimatization. Plant material consisted of shoot tips and nodes. Among the three cytokinins, benzyladenine, kinetin and 2-isopentenyl-adenine, only BA effectively stimulated the shoot multiplication from axiliary buds. The highest multiplication rate was obtained from nodes in the presence of 0.25– 0.5 mg·dm–3 BA. Higher concentrations shortened the internodes and decreased the leaf blades and growth of callus. 1 mg·dm–3 of KIN and 2iP positively influenced the shoot growth and size of leaves. Gibberellic acid (GA3) used with BA increased the number of auxillary shoots. The best quality shoots and the highest multiplication rate were obtained when 2 mg·dm–3 BA was used with 5 mg·dm–3 GA3. Cytokinins affected the rooting and acclimatization ex vitro. Dahlia shoots multiplicated in the presence of 1 mg·dm–3 KIN or 2iP rooted faster in the soil and 100% survived in field, while those from 1 mg·dm–3 BA media rooted slowly, had shorter shoots and only 60% of them survived. Plants bloomed after 11–12 weeks in the field. Dahlia plants that had been multiplicated in the presence of KIN had larger diameter and fresh weight in the field. BA and 2iP positively influenced the flower diameter, length of flower stalk and a number of the first-order shoots.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 5; 49-61
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Micropropagation of an old specimen of common lilac [Syringa vulgaris L.] from the Dendrological Garden at Przelewice
    Mikrorozmnażanie starego okazu lilaka zwyczajnego (Syringa vulgaris L.) z Ogrodu Dendrologicznego w Przelewicach
    Autorzy:
    Nesterowicz, S.
    Kulpa, D.
    Moder, K.
    Kurek J.
    Powiązania:
    https://bibliotekanauki.pl/articles/11364536.pdf
    Data publikacji:
    2006
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    in vitro culture
    Dendrological Garden in Przelewice
    common lilac
    regeneration process
    growth regulator
    micropropagation
    plant age
    old specimen
    Syringa vulgaris
    Opis:
    The published results on plant propagation m vitro culture show the importance of maternal plant age in a successful regeneration process. It is known that initiation effectiveness is low when primary explants are taken from old trees. The aim of this study was an attempt to regenerate Syringa vulgaris from an old specimen of Dendrological Garden in in vitro culture. April turned out to be the optimal month for collecting explants. The highest percentage of initiated shoots were obtained on MS medium containing 7.5 mg⋅dm⁻³ BAP and 0.02 mg⋅dm⁻³ NAA. The highest propagation efficiency was received on MS medium with a double amount of MgSO₄ supplemented with 1.0 mg⋅dm⁻³ BAP. Insignificant effects were observed on the media with cytokinin BAP. The plants with the best-developed root system were obtained on MS medium with reduced to ¼ amount of macro- and micronutrients supplemented with 5.0 mg·dm⁻³ IBA.
    W badaniach nad rozmnażaniem roślin w kulturach in vitro podkreśla się wpływ wieku rośliny matecznej na powodzenie procesu regeneracji. Efektywność inicjacji kultur ze starych okazów drzew jest niska. Celem badań była próba regeneracji w kulturach in vitro lilaka zwyczajnego Syringa vulgaris z okazu mającego 80 lat. Optymalnym terminem pozyskiwania eksplantatów z kolekcji był kwiecień. Najlepsze wyniki na etapie inicjacji otrzymano na pożywce MS uzupełnionej 7,5 mg·dm⁻³ BAP i 0,02 mg·dm⁻³ NAA. Najwyższy współczynnik rozmnożenia roślin otrzymano na pożywce MS z podwojoną ilością MgSO₄ i uzupełnionej 1,0 mg·dm⁻³ BAP. Nieistotnie niższe wyniki otrzymano na pozostałych pożywkach zawierających BAP w swoim składzie. Najlepiej rozwinięty system korzeniowy obserwowano u roślin ukorzenianych na pożywce zawierającej ¼ makro- i mikroelementów pożywki MS, uzupełnionej 5,0 mg·dm⁻³ IBA.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2006, 05, 1; 27-35
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-12 z 12

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