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Wyświetlanie 1-2 z 2
Tytuł:
Effect of meta-topolin in vitro propagation of Pelargonium x hortorum and Pelargonium x hederaefolium cultivars
Autorzy:
Wojtania, A
Powiązania:
https://bibliotekanauki.pl/articles/57180.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
meta-topolin
in vitro propagation
micropropagation
cytokinin
shoot formation
root formation
Pelargonium x hortorum
Pelargonium x hederaefolium
plant cultivar
Opis:
July and September. The most efficient regeneration and axillary multiplication were achieved on the medium supplemented with meta-topolin. The application of BAP caused a lower regeneration potency of explants and resulted in a decrease of shoot quality with every subculture. Four of the six cultivars showed growth inhibition after three months of growth on BAP-medium. The highest multiplication rate (2.7-4.7 depending on genotype) and the high quality of shoots were noted on the medium supplemented with mT (0.5-1.0 mg l-1). It is also very important to note that mT had stimulating effect on organogenesis in P. × hederaefolium and P. × hortorum cultivars over the long term. Moreover, meta-topolin had no after-effect on the growth and inhibition of rooting. Only one cultivar ("Sofie Cascade") rooted better on control medium without auxin. In case of the other cultivars, IBA added in concentrations of 0.01-0.1 mg l-1 had a stimulating effect on root production. The higher level of auxin inhibited root formation, stimulated senescence of shoots and had a negative after-effect on acclimatization in greenhouse conditions.
Źródło:
Acta Societatis Botanicorum Poloniae; 2010, 79, 2; 101-106
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ex vitro rooting, acclimatization and genetic stability of Lonicera caerulea var. kamtschatica
Autorzy:
Wojtania, A.
Markiewicz, M.
Goraj-Koniarska, J.
Powiązania:
https://bibliotekanauki.pl/articles/1077947.pdf
Data publikacji:
2020
Wydawca:
Instytut Ogrodnictwa
Tematy:
rooting
acclimatization
genetic stability
Lonicera caerulea var.kamtschatica
blue honeysuckle
ex vitro
amplified fragment length polymorphism
inter simple specific repeat
micropropagation
Opis:
Ex vitro rooting and acclimatization of two cultivars ‘Wojtek’ and ‘Zojka’ of blue honeysuckle (Lonicera caerulea var. kamtschatica Sevast.) were studied. To the ex vitro conditions were transferred rooted and unrooted shoots. The post-effect of auxin type and concentration as well as microcutting and soil substrate types were tested. The genetic stability of the plantlets in relation to the mother plants by using amplified fragment length polymorphism (AFLP) and inter simple sequence repeat (ISSR) markers has been also determined. It has been found that in vitro rooted cuttings of both cultivars showed a higher survival rate (max. 88%) and better growth and development when they were rooted on a medium containing a low auxin level (1.0 mg·dm-3). The results of the second experiment showed successful ex vitro rooting of blue honeysuckle shoots without auxin treatment. Higher ex vitro rooting and survival rate in the greenhouse have been observed for ‘Wojtek’ (max. 96%) than ‘Zojka’ (max. 88%). Better growth and development of shoots and roots were observed on peat alone or a mixture of peat and perlite as compared to a mixture of peat and sand. The micropropagated plantlets appeared similar to mother plants. Molecular analysis confirmed a high level of genetic stability of blue honeysuckle after 2 years of in vitro propagation. However, among the cultivars studied, ‘Wojtek’ showed slightly higher genetic stability than ‘Zojka’ (99.5% and 97.7%, respectively). For ‘Zojka’ plants, the degree of variation was comparable for AFLP and ISSR markers. For ‘Wojtek’, no polymorphism was detected using the ISSR analysis in contrast to the AFLP analysis.
Źródło:
Journal of Horticultural Research; 2020, 28, 2; 61-70
2300-5009
Pojawia się w:
Journal of Horticultural Research
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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