Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

English (EN)·Polski (PL)·Yкраїнський (UK)
Przejdź do strony domowej biblioteki Centralna Biblioteka Wojskowa Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaCentralna Biblioteka Wojskowa

Wyszukujesz frazę "in vitro cultivation" wg kryterium: Wszystkie pola

  Lista filtrów
Wyrównaj
    Wyświetlanie 1-7 z 7
    Tytuł:
    In vitro multiplication and acclimatization of Biscutella laevigata (Brassicaceae) to cultivation in greenhouse conditions
    Autorzy:
    Hanus-Fajerska, E.
    Wiszniewska, A.
    Muszynska, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/80691.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Biscutella laevigata
    biostimulator
    in vitro
    propagation
    multiplication
    micropropagation
    cultivation
    greenhouse condition
    vegetative propagation
    photosynthetic photon flux density
    plant growth regulator
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2012, 93, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Environmental and ecological aspects of cultivation of selected energy and herbal plants propagated by in vitro culture
    Autorzy:
    Tomaszewska-Sowa, M.
    Figas, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/61499.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Stowarzyszenie Infrastruktura i Ekologia Terenów Wiejskich PAN
    Tematy:
    environmental aspect
    ecological aspect
    plant cultivation
    herbal plant
    micropropagation
    in vitro culture
    growth regulation
    Silphium perfoliatum
    Helichrysum arenarium
    Stevia rebaudiana
    Opis:
    There has been a growing interest over the last years in techniques using alternative and pharmaceutical plants due to their wide potential applications. These species are characterised by valuable and multidirectional usable features, due to which their practical application is superior to their physiognomic features. These plants may provide a raw material for production of medicines, cosmetics or may be used for animal feeds. Moreover they may be used as ornamental, melliferous and energy plants. Owing to their generally low soil requirements they are used in the reclamation of degraded land. Among the species characterized by multidirectional use are Silphium perfoliatum, Helichrysum arenarium and Stevia rebaudiana. These plants are characterized by a high content of active compounds and a number of pro-health characteristics. Because raw materials for production of medicines and cosmetics should be standardized, cultures maintained under controlled conditions are a good source of seedlings.
    Źródło:
    Infrastruktura i Ekologia Terenów Wiejskich; 2014, IV/3
    1732-5587
    Pojawia się w:
    Infrastruktura i Ekologia Terenów Wiejskich
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cultivation of soapwort plants (Saponaria officinalis L.) in in vitro cultures
    Autorzy:
    Figas, Anna
    Lefelbajn, Weronika
    Powiązania:
    https://bibliotekanauki.pl/articles/16243498.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Stowarzyszenie Infrastruktura i Ekologia Terenów Wiejskich PAN
    Tematy:
    medicinal plant
    axillary shoots
    micropropagation
    plant growth regulator
    Opis:
    Saponaria officinalis L. is a medicinal plant from the family Caryophyllaceae. It occurs in most European countries, as well as in North Africa, America and Western Asia. The aim of the study was to find an optimal method of sterilization S. officinalis seeds in order to obtain sterile seedlings and to multiply axillary shoots from nodal explants isolated from them, on MS medium with the addition of growth regulators (RW). In this study, 4 variants of seed sterilization were compared using NaClO at the following concentrations: 1.5% (2), 2% (3) and 2.5% (4) for a period of 11 minutes. The control was a variant in which 70% C2H5OH was used for pre-sterilization for 1 minute (1). The highest percentage of sprouted, seeds and sterile seedlings and the lowest percentage of contamination were obtained in variant (4). As a result of micropropagation of soapwort in in vitro cultures, axillary shoots were formed from nodal explants from axillary buds. The highest percentage of explants with shoots (95%) and callus tissue (48%) and the highest number of shoots from one explant (5.95) were obtained in the second passage on MS medium with the addition of 4 mg.dm-3 BAP and 0.5 mg.dm-3 NAA. Due to the wide possibilities of using soapwort, it is advisable to continue research aimed at developing an optimal and efficient plant regeneration system of this species.
    Źródło:
    Infrastruktura i Ekologia Terenów Wiejskich; 2023, I/1; 59--69
    1732-5587
    Pojawia się w:
    Infrastruktura i Ekologia Terenów Wiejskich
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro proliferation and ex vitro rooting of microshoots of lisianthus using auxin and cytokinin on solid, liquid and double-phase culture systems
    Autorzy:
    Kaviani, B.
    Bahari, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/12678778.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    Gentianaceae
    Eustoma
    Lisianthus
    Eustoma grandiflorum
    ornamental plant
    flowering plant
    rooting
    microshoot
    micropropagation
    in vitro proliferation
    ex vitro method
    soild phase
    liquid phase
    double-phase culture
    plant growth regulator
    auxin
    cytokinin
    Opis:
    A protocol was developed for high frequency and low cost of in vitro shoot proliferation and ex vitro rooting of Eustoma grandiflorum (Gentianaceae) on solid medium. Shoot tips as explants were cultured on Murashige and Skoog (MS) medium enriched with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.00, 0.01, 0.10 and 1.00 mg l–1) and 6-benzylaminopurine (BAP) (0.00, 0.50, 2.00 and 5.00 mg l–1). Three culture media systems (solid, liquid and double-phase) were applied. None of the explants cultured on liquid and double-phase media resulted in live plant production. Maximum axillary shoot number (54.45) was recorded in the plantlets treated with 0.10 mg l–1 2,4-D in combination with 5.00 mg l–1 BAP. Treatment of 0.01 mg l–1 2,4-D along with 0.50 mg l–1 BAP produced maximum node number and internode length. Some shoots produced on medium containing plant growth regulators (PGRs) were rooted in soil. The largest number (5.50/plantlet) and longest length of root (7.75 cm/plantlet) were obtained in ex vitro condition on the base of shoots produced in culture medium enriched with 0.10 mg l–1 2,4-D along with 0.50 mg l–1 BAP. The combination of 1.00 mg l–1 2,4-D and 0.50 mg l–1 BAP was found to be the most suitable PGRs for obtaining the highest callus weight. The most fresh weight was calculated from plantlets grown on the medium containing 0.10 mg l–1 2,4-D along with 5.00 mg l–1 BAP. Maximum dry weight was obtained in free-PGRs medium. About 90% of the rooted plantlets were established successfully in cultivation beds. Acclimatized plants were morphologically similar to the mother plants.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 47-56
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Sustainable micropropagation of selected Stevia rebaudiana Bertoni genotypes
    Autorzy:
    Kaplan, B.
    Duraklioglu, S.
    Turgut, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/12691030.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    Turkey
    plant cultivation
    Compositae
    Stevia rebaudiana
    steviol glycoside
    sweetener
    micropropagation
    in vitro propagation
    growth regulator
    genotype
    Gelrite preparation
    Opis:
    Stevia rebaudiana Bertoni is a perennial plant belonging to Asteraceae family and its leaves contain steviol glycosides (SGs) that are 150 to 300 times sweeter than sucrose. The sweeteners obtained from S. rebaudiana can be safely used by diabetics as insulin secretion is not required during digestion of this sweetener. As it has zero calories, it is also used in diet products. Adaptation studies for Stevia conducted in Antalya, Turkey have shown that the stevia plant could easily be cultivated as a perennial. However, the lack of a sustainable vegetative propagation method creates a significant problem for stevia production. In the generatively populations, homogeneity and therefore quality are decreased because of cross-pollination. Stevia, as a self-incompatible and cross-pollinated species, has been shown to have very high genetic diversity. Therefore, development of a sustainable in vitro propagation method to prevent genetic heterogeneity of selected varieties is crucial for stevia cultivation. The aim of this study was to evaluate 2 different gelling agents (plant agar and Gelrite) and 20 different growth regulators combinations. The results demonstrated an approximately 200-fold multiplication rate obtained within 13 weeks using MS medium supplemented with 0.5 mg·dm–3 BAP and 0.25 mg·dm–3 kinetin and solidified with Gelrite. Average stevioside and rebaudioside A contents in in vitro propagated plant samples were found to be 8.1% and 8.6%, respectively.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 6; 47-56
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Fast direct regeneration of plants from nodal explants of Stevia rebaudiana Bert.
    Autorzy:
    Doliński, R.
    Kowalczyk, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/12611010.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    Stevia rebaudiana
    plant regeneration
    plant use
    medicinal plant
    nodal explant
    micropropagation
    in vitro culture
    auxin concentration
    Opis:
    In the preceding research, stevia has been typically cloned in vitro using two media, on which the shoots were formed (3–6 weeks), and on the other they were rooted (3–5 weeks). This study aimed at finding the possibility for rapid stevia propagation from large nodal explants using the MS basal medium [Murashige and Skoog 1962], with low auxin concentrations (0.5, 1 and 2 mg.dm–3). The plants were obtained as soon as after three weeks. The best results were obtained from media with various concentrations of the indole-3-acetic acid (IAA) and the highest concentration of phenylacetic acid (PAA). Plants were formed by 83.9–86.0% of explants, they had high weight (234–253 mg), two shoots measuring 2.07–2.37 cm and 5.8–8.3 roots measuring 1.00–1.24 cm. Mean plant weight was the lowest on media with indole-3-butyric acid (IBA) (185–192 mg). Both explant buds formed single shoots, but their development was typically uneven. The differences in the length and weight of shoots were the lowest on media with IAA and at lower PAA concentrations. Plants from the media with IAA and the control medium were distinguished by higher number of nodes. The percentage share of shoots in the total plant weight was the highest on media with PAA (62.1–62.7%), and the lowest at higher concentrations of α-naphthaleneacetic acid (NAA) (47.9 and 48.9%). Parts of explants immersed in media developed callus, and the highest amounts of this tissue were found in the media with NAA. 92.3% of plants survived the acclimatization. The applied procedure may be used for rapid in vitro cloning of selected stevia genotypes. The use of one medium enables reduction of seedling production costs. Moreover, cyclical cloning and extending the production scale is possible.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 5; 95-103
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of growth regulators on dahlia propagation in tissue culture and acclimatization of plants in ex vitro conditions
    Autorzy:
    Marcinek, B.
    Parzymies, M.
    Poniewozik, M.
    Kozak, D.
    Durlak, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/12307379.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    Dahlia
    Dahlia x cultorum
    dahlia
    ornamental plant
    flowering plant
    tuberous plant
    tuberous root
    plant acclimatization
    growth regulator
    cytokinin
    gibberellic acid
    micropropagation
    tissue culture
    ex vitro method
    microcutting
    Opis:
    The aim of the work was to evaluate the influence of cytokinins on in vitro propagated dahlia and their consequent effect on acclimatization. Plant material consisted of shoot tips and nodes. Among the three cytokinins, benzyladenine, kinetin and 2-isopentenyl-adenine, only BA effectively stimulated the shoot multiplication from axiliary buds. The highest multiplication rate was obtained from nodes in the presence of 0.25– 0.5 mg·dm–3 BA. Higher concentrations shortened the internodes and decreased the leaf blades and growth of callus. 1 mg·dm–3 of KIN and 2iP positively influenced the shoot growth and size of leaves. Gibberellic acid (GA3) used with BA increased the number of auxillary shoots. The best quality shoots and the highest multiplication rate were obtained when 2 mg·dm–3 BA was used with 5 mg·dm–3 GA3. Cytokinins affected the rooting and acclimatization ex vitro. Dahlia shoots multiplicated in the presence of 1 mg·dm–3 KIN or 2iP rooted faster in the soil and 100% survived in field, while those from 1 mg·dm–3 BA media rooted slowly, had shorter shoots and only 60% of them survived. Plants bloomed after 11–12 weeks in the field. Dahlia plants that had been multiplicated in the presence of KIN had larger diameter and fresh weight in the field. BA and 2iP positively influenced the flower diameter, length of flower stalk and a number of the first-order shoots.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 5; 49-61
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-7 z 7

      Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies