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Tytuł:
Określenie pochodzenia wyłączonych drzewostanów nasiennych sosny rychtalskiej (Pinus sylvestris L.) z wykorzystaniem markerów mikrosatelitarnych
Determination of the origin of the rychtal Scots pine (Pinus sylvestris L.) seed tree stands using microsatellite markers
Autorzy:
Wójkiewicz, B.
Żukowska, W.B.
Urbaniak, L.
Kowalczyk, J.
Litkowiec, M.
Lewandowski, A.
Powiązania:
https://bibliotekanauki.pl/articles/985719.pdf
Data publikacji:
2019
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
hodowla lasu
sosna zwyczajna
Pinus sylvestris
sosna rychtalska
drzewostany nasienne
pochodzenie roslin
zmiennosc genetyczna
analiza DNA
markery mikrosatelitarne
scots pine
genetic variation
gene pool
genetic structure
ssr markers
Opis:
The rychtal pine is one of the most valuable ecotypes of Scots pine (Pinus sylvestris L.) approved for the breeding purposes in Poland. However, it occupies stands typical for oaks and beeches as shown by the compatibility analysis of species composition in relation to the habitat type in which they occur. Such result raises some doubts in terms of the naturalness of the rychtal pine and calls its history and origin into question. In the present study, we used the set of nuclear microsatellite markers to characterize and compare the gene pool composition of the selected seed tree stands of the rychtal pine with 200−year−old pine trees which grow at the Syców Forest District (SW Poland). We aimed to know to what extent the set of alleles specified for the group of the oldest trees from natural habitats is represented in the younger forest tree stands of the rychtal pine. The analysis of molecular variance (AMOVA) and clustering analysis showed that the gene pool of the studied pine populations was homogenous (FST=0,02%, K=1). The parameters of genetic variation were similar for all populations except for the mean number of alleles. On average, 25 new alleles were found in two rychtal pine seed tree stands as compared to the set of alleles found in the group of old pine trees. However, all alleles defined for old pines were also present in the gene pool of younger rychtal pine forest stands. The differences in the gene pool richness result most likely from quite high differences in the number of individuals analyzed from each population. In conclusion, our results indicate the common origin of the studied Scots pine populations.
Źródło:
Sylwan; 2019, 163, 08; 637-644
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Stan zdrowotny a zróżnicowanie genetyczne wybranych drzewostanów świerkowych na terenie RDLP w Krośnie
Genetic variability and health of Norway spruce stands in the Regional Directorate of the State Forests in Krosno
Autorzy:
Gutkowska, J.
Borys, M.
Tereba, A.
Tkaczyk, M.
Oszako, T.
Nowakowska, J.A.
Powiązania:
https://bibliotekanauki.pl/articles/1293265.pdf
Data publikacji:
2017
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
lesnictwo
RDLP Krosno
drzewostany swierkowe
stan zdrowotny lasu
drzewa lesne
swierk pospolity
Picea abies
zroznicowanie genetyczne
markery molekularne
markery mikrosatelitarne
DNA mitochondrialny
Norway spruce
health state
SSR markers
mtDNA
genetic diversity
Opis:
The study was conducted in 2015 in six spruce stands situated in different forest districts administratively belonging to the Regional Directorate of Forests State in Krosno. Each spruce population was represented by 30 trees and assessed in terms of their current health status. Genetic analyses were performed based on shoot samples from each tree using nine nuclear DNA markers and one mitochondrial DNA marker (nad1). The health status of the trees was described according to the classification developed by Szczepkowski and Tarasiuk (2005) and the correlation between health classes and the level of genetic variability was computed with STATISTICA (α = 0.05). Nuclear DNA analyses revealed a low level of genetic variability among spruce populations (only 3% of the total genetic variation (FST = 0.028) and a high variability within populations (97%). The total heterozygosity in all stands (HT) was calculated as 0.646. Based on UPGMA analysis, the most genetically similar populations are spruce stands in the Bieszczady National Park and the Ustrzyki Dolne Forest District, which have the smallest genetic divergence of all populations (DN = 0.0165). Our analysis of the mitochondrial gene nad1 revealed the presence of six different haplotypes “a”, “a1”, “b”, “c”, “d” and “d1”. Comprising 56% of all haplotypes, “a” was the most common showing a predominant impact on spruce migration from the Carpathian area. The analysis based on mitochondrial markers (by Nei) revealed a heterozygosity of 0.525. Based on the observations of disease symptoms, 29% of the trees belong to health class 1,30% to class 2,28% to class 3 and class 4 contains 13% of trees. The comparison between health status and the level of genetic variation in the analyzed stands showed a positive correlation. Spruce stands with better health were also characterized by a greater degree of genetic variability. Since most of the investigated spruce populations shared the mitochondrial haplotype “a”, we have ascertained their Hercynian- Carpathian origin. Only one stand (Cisna) had a high frequency (43.3%) of the Nordic haplotype “c” suggesting that this provenance is derived from the Baltic post-glacial refugium of P. abies in Europe.
Źródło:
Leśne Prace Badawcze; 2017, 78, 1
1732-9442
2082-8926
Pojawia się w:
Leśne Prace Badawcze
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Porównanie zmienności genetycznej pokolenia matecznego i sztucznie wyhodowanego potomstwa sosny zwyczajnej na podstawie analiz DNA
Comparison of the genetic variability of Scots pine trees and their progeny from nursery production based on DNA analyses
Autorzy:
Konecka, A.
Tereba, A.
Bieniek, J.
Nowakowska, J.A.
Powiązania:
https://bibliotekanauki.pl/articles/985843.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
hodowla lasu
sosna zwyczajna
Pinus sylvestris
zmiennosc genetyczna
analiza DNA
markery mikrosatelitarne
drzewa mateczne
drzewa potomne
sadzonki z zakrytym systemem korzeniowym
genetic diversity
ssr markers
forest nursery production
pinus sylvestris l.
Opis:
The production of forest tree species in forest nurseries is performed via two main breeding systems: i) the traditional (conventional) way with the seedlings grown in soil, and ii) plants cultivated in the containers. The aim of the study was to assess the level of genetic variability in the populations of the mother stands and the progeny populations of Scots pine cultured with traditional way (in soil) and in containers in two nurseries in Olsztynek (N Poland) and Oleszyce (S Poland) forest districts. Four polymorphic microsatellite markers (SPAG 7.14, SPAC 11.6, SPAC 12.5 and SsrPt_ctg4363) were used to evaluate the genetic variability of the studied populations. The basic hypothesis assumed that higher gene pool characterizes the seedlings grown in the containers comparing to the seedlings grown in the ground. The results confirmed that. Seedlings from containerized breeding had larger gene pool and were more diverse than plants with conventional breeding, both in Olsztynek and Oleszyce. Our study revealed a significant human impact on shaping the pool of forest genetic resources of Polish forests at the early stage of nursery production and showed the need for a broader study on further stages of cultivation of forests.
Źródło:
Sylwan; 2018, 162, 01; 32-40
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zróżnicowanie fenotypowe i zmienność tła genetycznego polskich proweniencji sosny zwyczajnej
Phenotypic differentiation and genetic background variation of Polish provenances of Pinus sylvestris L.
Autorzy:
Hebda, A.
Skrzyszewski, J.
Wachowiak, W.
Powiązania:
https://bibliotekanauki.pl/articles/987201.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
hodowla lasu
drzewa lesne
sosna zwyczajna
Pinus sylvestris
proweniencje krajowe
zmiennosc fenotypowa
zmiennosc genetyczna
markery mikrosatelitarne
DNA mikrosatelitarny
polimorfizm loci
zmiennosc adaptacyjna
scots pine
phenotypic differentiation
neutral variation
microsatellite markers
environment correlation
local adaptation
Opis:
We looked at the patterns of differentiation at a set of phenotypic traits of Scots pine populations derived from different environmental conditions of the species distribution range in Poland. The growth traits were tested over 47 years at the provenance trial experiment in the Carpathians. The phenotypic differentiation of the populations was compared to diversity estimates at a set of ten nuclear microsatellite markers. Scots pine populations were highly differentiated concerning diameter at breast high, stand volume, stem straightness and crown width according to the prove− nance and climatic zones. At the same time a low genetic diversity for microsatellite markers and no evidence of population structure was found among proveniences. Local populations from the Carpathian Mountains had lower genetic variation and weaker growth compared to lowland provenances. Among the tested populations Scots pine from lowlands were characterized by the great growth potential and good adaptation to the severe climate of the Carpathians. Populations that showed high quantitative traits differentiation were genetically homogenous at the neutral loci. The results indicate that there are some regions in the genome under selection that drive species adaptive variation.
Źródło:
Sylwan; 2017, 161, 04; 277-286
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
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