- Tytuł:
- Monitoring vital functions of A-375 melanoma cell cultures via thin-film nickel capacitors
- Autorzy:
-
Wilczyńska, Aleksandra
Kociubiński, Andrzej
Zarzeczny, Dawid
Szypulski, Maciej
Pigoń, Dominika
Małecka-Massalska, Teresa
Prendecka-Wróbel, Monika - Powiązania:
- https://bibliotekanauki.pl/articles/1844972.pdf
- Data publikacji:
- 2020
- Wydawca:
- Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
- Tematy:
-
ECIS
nickel
thin layers
melanoma
magnetron sputtering - Opis:
- This article deals in the constantly developing branch of microelectronic devices used in various fields of medicine, i.e. diagnostics and treatment of previously incurable human diseases. A method for assessing and monitoring the vital functions of living cells by measuring cellular impedance in real-time using the ECIS® system and a commercial culture substrate is presented. The goal was to develop a substrate significantly less expensive than a commercial substrate that would be suitable for multiple uses and compatible with the ECIS® measurement station. Moreover, thanks to the use of a material with electrochemical properties other than the biocompatible material (gold or platinum) it is possible to observe the cells behavior with regard to the toxic agent. For this purpose, a culture substrate with nickel comb capacitors was used. To make the electrodes, a thin metal layer was sputtered on polycarbonate plates in the magnetron sputtering process. Prior to the next stages, technological masks were designed so as to fit in the ECIS® measuring station. Subsequently, the microelectronic processes of photolithography and etching the metal layer were performed. Finally, the wells were glued onto the culture medium with a biocompatible adhesive. The completed substrates were transferred to the Department of Human Physiology, Medical University of Lublin, for the culture test on A-375 human melanoma cells. The results of the experiment determined the usefulness of the device for monitoring cell culture vital functions by means of impedance measurement.
- Źródło:
-
Engineering of Biomaterials; 2020, 23, 157; 10-14
1429-7248 - Pojawia się w:
- Engineering of Biomaterials
- Dostawca treści:
- Biblioteka Nauki
Artykuł