Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

English (EN)·Polski (PL)·Yкраїнський (UK)
Przejdź do strony domowej biblioteki Centralna Biblioteka Wojskowa Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaCentralna Biblioteka Wojskowa

Wyszukujesz frazę "apoptosis" wg kryterium: Temat

  Lista filtrów
Wyrównaj
    Wyświetlanie 1-6 z 6
    Tytuł:
    Helicobacter pylori increases expression of proapoptotic markers Fas and FasL on CD4 lymphocytes in children
    Autorzy:
    Kotłowska-Kmieć, Aldona
    Bąkowska, Alicja
    Szarszewski, Adam
    Kamińska, Barbara
    Łuczak, Grażyna
    Radys, Wojciech
    Landowski, Piotr
    Brodzicki, Jacek
    Korzon, Maria
    Liberek, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1040535.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    T cell
    H. pylori
    FasL
    Fas
    apoptosis
    lymphocyte
    Opis:
    The pathomechanism of Helicobacter pylori action upon gastric mucosa and its role in the pathogenesis of gastritis have not been fully elucidated. The aim of this study was to evaluate the most prevalent lymphocyte subpopulations of the gastric mucosa in gastritis in children, as well as to evaluate the expression of Fas and Fas ligand receptors (FasL), periapoptotic markers of gastric mucosa lymphocytes before and after H. pylori eradication. Forty nine patients aged 6 to 17 years, investigated due to chronic abdominal pain, were studied. The obtained tissue samples were analysed by immunohistochemistry. Different lymphocyte subsets were quantified on the basis of surface antigen expression (CD3, CD4, CD8, CD20), secreted cytokines (IL-4, IL-6, IFNγ) and Fas and FasL proteins in the gastric mucosa. B and T helper lymphocytes were found to play a major role in the inflammatory infiltration in the gastric mucosa in children during H. pylori infection. Their expression was found to decrease after eradication. The enhanced expression of Fas receptor on lymphocytes before treatment and a decrease of this expression after eradication of H. pylori were shown. It was demonstrated that there is a correlation between CD4 and Fas receptor expression that may induce apoptosis of the helper lymphocytes in infected children.
    Źródło:
    Acta Biochimica Polonica; 2009, 56, 3; 433-438
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Changes in phosphorylation of histone H2A.X and p53 in response of peripheral blood lymphocytes to gamma irradiation
    Autorzy:
    Vilasová, Zdeňka
    Řezáčová, Martina
    Vávrová, Jiřina
    Tichý, Aleš
    Vokurková, Doris
    Zoelzer, Friedo
    Řeháková, Zuzana
    Osterreicher, Jan
    Lukášová, Emilie
    Powiązania:
    https://bibliotekanauki.pl/articles/1040760.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    lymphocyte
    ionizing radiation
    p53
    phytohemagglutinin (PHA)
    apoptosis
    DNA damage
    Opis:
    The main aim of this study was to compare the reaction of quiescent and proliferating, i.e. phytohemagglutinin (PHA)-stimulated, human peripheral blood mononuclear cells (PBMCs) to γ-radiation, and analyse changes of proteins related to repair of DNA damage and apoptosis, such as γH2A.X, p53, p53 phosphorylation at serines-15 and -392, and p21 and their dose dependence. Freshly isolated PBMCs in peripheral blood are predominantly quiescent, in G0 phase, and with very low amounts of proteins p53 and p21. Using confocal microscopy we detected dose dependent (0.5-5 Gy) induction of foci containing γH2A.X (1 h after γ-ray exposure), which are formed around radiation-induced double strand breaks of DNA. Apoptosis was detected from 24 h after irradiation by the dose of 4 Gy onwards by Annexin V binding and lamin B cleavage. Seventy two hours after irradiation 70% of CD3+ lymphocytes were A+. Neither increase in p53 nor its phosphorylation on serine-392 after irradiation was detected in these cells. However, massive increase in p21 (cyclin-dependent kinase inhibitor 1A) was detected after irradiation, which can be responsible for late occurrence of apoptosis in these quiescent cells. PHA-stimulation itself (72 h) caused an increase in early apoptosis (A+PI-) in comparison to non-stimulated PBMCs (38% A+ resp. 13.4%). After PHA-stimulation also the amount of γH2A.X, p53, and p21 increased, but no phosphorylation of p53 on serine-392 or -15 was detected. Reaction to γ-radiation was different in PHA-stimulated lymphocytes: the p53 pathway was activated and p53 was phosphorylated on serines-15 and -392 4 h after irradiation by the dose of 4 Gy. Phosphorylation of p53 at serine-15 increased in a dose-dependent manner in the studied dose range 0.2-7.5 Gy. Also the amount of p21 increased after irradiation. Seventy two hours after irradiation of PHA-stimulated CD3+ T lymphocytes by the dose of 4 Gy 65% of cells were A+.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 2; 381-390
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of different lymphocyte-bacteria ratios and bacterial toxins on apoptosis of bovine mammary gland lymphocytes
    Wpływ stosunku stężeń limfocytów do bakterii oraz toksyn bakteryjnych na śmierć limfocytów sutków jałówek
    Autorzy:
    Slama, P.
    Sladek, Z.
    Rysanek, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/127193.pdf
    Data publikacji:
    2011
    Wydawca:
    Towarzystwo Chemii i Inżynierii Ekologicznej
    Tematy:
    bacteria
    bacterial toxin
    apoptosis
    lymphocyte
    mammary gland
    bakterie
    toksyny bakteryjne
    apoptoza limfocytów
    gruczoł sutkowy
    Opis:
    Staphylococcus aureus and Streptococcus uberis delay apoptosis of bovine mammary gland lymphocytes following intramammary infusion and in in vitro studies with lymphocyte-bacteria ratio 1:1. In this study, we investigated the effect of different lymphocyte-bacteria ratios on apoptosis of bovine mammary gland lymphocytes in vitro. We found out that lymphocyte-bacteria (S. aureus or S. uberis) ratios 1:10, 1:50 and 1:100 have different effect on apoptosis of lymphocytes than ratio 1:1. Lymphocyte apoptosis was induced 6 hours following incubation with S. aureus or S. uberis with mentioned ratios (1:10, 1:50 and 1:100). In our previous preliminary experiments focused on exploration of chemical components of bacteria on apoptosis of lymphocytes, we established the effect of muramyl dipeptide and lipopolysaccharide on lymphocyte apoptosis only in vitro. Therefore, in the second part of the present study we focused our experiments on investigation of the effect of Gram-negative bacterial toxin lipopolysaccharide on apoptosis of bovine mammary gland lymphocytes in vivo. The results of these experiments suggest that lipopolysaccharide induces apoptosis of lymphocytes following intramammary application. These data need next exploration to reveal detail effects of bacteria or bacterial toxins on lymphocyte programmed cell death in connection with inflammatory process.
    Źródło:
    Proceedings of ECOpole; 2011, 5, 1; 109-113
    1898-617X
    2084-4557
    Pojawia się w:
    Proceedings of ECOpole
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Anatoxin-a induces apoptosis of leukocytes and decreases the proliferative ability of lymphocytes of common carp (Cyprinus carpio L.) in vitro
    Autorzy:
    Bownik, A.
    Rymuszka, A.
    Sieroslawska, A.
    Skowronski, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/31172.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    anatoxin
    apoptosis
    leucocyte
    proliferative ability
    lymphocyte
    common carp
    Cyprinus carpio
    in vitro culture
    immunotoxicity
    fish
    anatoxin-a
    cyanotoxin
    necrosis
    Opis:
    Cyanobacteria (Cyanophyta, Cyanoprocaryota, Cyanobacteria) (blue-green algae) are procaryotic phototrophic microorganisms playing an important ecological role in the freshwater and marine environment as primary producers. However, as a consequence of water eutrophication observed in many reservoirs in different parts of the world, these microorganisms form massive scums, known as water blooms, releasing cyanotoxins hazardous to fish and other aquatic organisms. Cyanotoxins are cyanobacterial secondary metabolites of various chemical structures harmful to humans, terrestial and aquatic animals such as fish. The most abundant cyanotoxins are microcystins and hepatotoxins inducing toxic changes in fish liver, kidney, gills, digestive tract and immune system. Very little is known on the effects of alkaloid neurotoxic anatoxin-a on fish and their immunity. The aim of this study was to assess the in vitro influence of anatoxin-a on immune cells isolated from the common carp (Cyprinus carpio L.). The leukocyte intracellular level of ATP was reduced only at the highest concentration of anatoxin-a. Apoptotic and necrotic leukocytes were observed at the lower and the highest concentrations of anatoxin-a, respectively. Elevated activity of caspases 3/7 after 2 hours and a concentration-dependent decrease in the proliferative ability of T and B lymphocytes was also observed. The results suggest that anatoxin-a could be a possible immunotoxic agent in the aquatic environment and may increase the susceptibility of fish to infectious and neoplastic diseases. Therefore, constant monitoring of anatoxin-a and its producers in lakes and fish ponds should be performed.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Evaluation of the influence of meloxicam and flunixin meglumine on the apoptosis of peripheral blood CD4plus and CD8plus T cells in calves
    Autorzy:
    Maslanka, T
    Jaroszewski, J.J.
    Markiewicz, W.
    Jabubowski, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/31556.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    peripheral blood
    T cell
    calf
    CD4plus T cell
    CD8plus T cell
    apoptosis
    meloxicam
    flunixin meglumine
    lymphocyte
    antiinflammatory drug
    Opis:
    The aim of the study was to determine whether treatment with recommended doses of meloxicam or flunixin had an effect on the apoptosis of peripheral blood T lymphocytes in calves. The study was carried out on 4-5 months old calves (n = 24, 8 per group). Experimental animals were injected subcutaneously with a single dose of 0.5 mg . kg-1 of meloxicam or intravenously with 3 doses of 2.2 mg . kg-1 day-1 of flunixin. The non-treatment animals served as control. Blood samples were taken at day 0 and at days 1, 2, 3, 5, 7 and 14 after the first NSAIDs injection. Apoptosis was determined by flow cytometry using Annexin V-PE/7-AAD staining. The kinetic analysis of apoptosis in the total lymphocyte population, as well as in the CD4+ and CD8+ subsets did not reveal significant differences in the frequency of early apoptotic cells between control and experimental groups throughout the period studied. Although, 24 h after administration of the first dose of NSAIDs, late-stage apoptosis/necrosis was significantly increased in the total lymphocyte population (the meloxicam group), as well as in the CD4+ (the meloxicam group and the flunixin group) and CD8+ (the flunixin group) subsets of T cells. However, this disturbance was transient, relatively poorly expressed and, thus, unlikely to be of clinical significance. Our results indicate that the use of meloxicam or flunixin in accordance with the recommended dosage regimen in cattle do not have a clinically significant influence on apoptosis of peripheral blood T cells.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 1; 3-12
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Searching for in vitro biomarkers of susceptibility to prostate and cervical cancers by analysis of chromosomal instability, gamma-H2AX foci, polymorphisms in DNA repair genes and apoptosis
    Autorzy:
    Wegierek-Ciuk, A.
    Arabski, M.
    Kedzierawski, P.
    Florek, A.
    Solowiej, D.
    Gozdz, S.
    Lisowska, H.
    Kowalik, A.
    Kowalska, M.
    Wojcik, A.
    Polanska, J.
    Lankoff, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/3629.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    in vitro biomarker
    susceptibility
    prostate
    cervical cancer
    cancer
    patient
    human disease
    lymphocyte
    man
    ionizing radiation
    chromosome instability
    gamma-H2AX biomarker
    polymorphism
    DNA repair gene
    apoptosis
    Opis:
    Introduction and objective. According to the cancer epidemiology databases, cancer is the second leading cause of death in developing countries. Moreover, the WHO predicts a continuing increase in the incidence of cancer, extending this trend well into the next several decades. Hence, it seems obvious that the prediction of cancer susceptibility and early diagnosis is an important goal for modern biomedical sciences. The aim of this study is to clarify the value of chromosomal damage, capacity for the repair of double-strand breaks (DSBs), polymorphisms in DNA repair genes, and apoptosis as prognostic markers for prostate and cervical cancer. Materials and methods. 30 prostate cancer patients and 30 cervical cancer patients were enrolled into the study. In addition, 30 healthy female donors and 30 healthy male donors served as controls. The following endpoints were investigated: frequency of micronuclei, gamma-H2AX fluorescence, XRCC1 194C>T, XRCC1 399G>A, XRCC3 IVS5–14 A>G, OGG1 326 Ser>Cys polymorphisms and apoptosis. Results. Among all tested factors, only the homozygous variant (Arg/Arg) in XRCC1 (399 Arg/Gln) was strongly associated with prostate cancer risk, and only a low apoptotic response was connected with cervical cancer risk. The presented study confirmed a positive association between the frequency of MN and increased prostate and cervical cancer risk. However, such a biomarker is not cancer specific. In addition, the information gained by analyzing the gamma-H2AX fluorescence, as well apoptosis, had no value for predicting the risk of prostate and cervical cancers. Conclusions. The final conclusion of the study is that cancer susceptibility is a complex phenotype not readily detectable in relatively small studies by functional assays or analysis of SNP in few, selected genes.
    Źródło:
    Journal of Pre-Clinical and Clinical Research; 2015, 09, 2
    1898-2395
    Pojawia się w:
    Journal of Pre-Clinical and Clinical Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

      Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies