- Tytuł:
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Lipid and protein oxidation in the muscle tissue of rainbow trout after in vitro treatment by extracts derived from greater celandine (Chelidonium majus L.) collected from rural and urban aglomerations of Pomeranian region
Oksydacja lipidów i białek w tkance mięśniowej pstraga tęczowego po inkubacji in vitro z ekstraktami z glistnika jaskółcze ziele (Chelidonium majus L.) zebranego z wiejskich i miejskich terenów Pomorza - Autorzy:
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Stefanowski, Nataniel
Tkachenko, Halina
Kurhaluk, Natalia
Lukash, Oleksandr
Aksonov, Ievgenii
Buyun, Lyudmyla - Powiązania:
- https://bibliotekanauki.pl/articles/27322952.pdf
- Data publikacji:
- 2021
- Wydawca:
- Uniwersytet Pomorski w Słupsku
- Tematy:
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coastal regions
rainbow trout
muscle tissue
lipid peroxidation
protein damage
total antioxidant capacity - Opis:
- Consistent with our previous studies, we continue to evaluate the antioxidant potential of representatives belonging to the Papaveraceae family collected from the coastal region of northern Poland using different cell models, namely the muscle tissue of rainbow trout. Therefore, in the current study, oxidative stress biomarkers [2-thiobarbituric acid reactive substances (TBARS), carbonyl derivatives of oxidative modification of proteins (OMP), total antioxidant capacity (TAC)] were used for evaluating the antioxidant activity of extracts derived from stalks and roots of Chelidonium majus L. (CM) at final dose 2.5 mg/mL. The muscle tissue of rainbow trout (Oncorhynchus mykiss Walbaum) was used in the current study. The supernatant of the muscle tissue was used to incubate with extracts derived from stalks and roots of CM (in a ratio of 39:1, final concentration 2.5 mg/mL) at room temperature. The control samples (muscle tissue) were incubated with 100 mM Tris-HCl buffer (pH 7.2) (in a ratio of 39:1). The results of the current study revealed that extracts derived from stalks and roots of CM exhibited cytotoxic effects on cellular structures of muscle tissue by increasing the level of lipid peroxidation and decreased of the total antioxidant capacity. These results suggest the possibility of using CM extracts at a final dose of 2.5 mg/mL for pro-oxidant effects and warrant further studies to evaluate their therapeutic potential. The levels of aldehydic and ketonic derivatives of OMP were significantly decreased after in vitro incubation with the extracts derived from stalks and roots of CM collected only from rural agglomerations. Screening of plants for other biological activities, including antioxidant activity, is essential and may be effective in the search for preventive measures in the pathogenesis of some diseases, as well as in the prevention and treatment of some disorders in human and veterinary medicine.
- Źródło:
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Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline; 2021, 25; 45-60
1643-0115 - Pojawia się w:
- Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline
- Dostawca treści:
- Biblioteka Nauki
Artykuł