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    Wyświetlanie 1-4 z 4
    Tytuł:
    Roznicowanie izolatow Malassezia pachydermatis metoda RAPD
    RAPD differentiation of yeast like fungi Malassezia pachydermatis
    Autorzy:
    Lesniak, M
    Dworecka-Kaszak, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/840386.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Malassezia
    diagnostyka parazytologiczna
    grzyby
    metoda RAPD
    Malassezia pachydermatis
    parazytologia
    konferencje
    roznicowanie
    izolaty grzybowe
    Warszawa konferencja
    Opis:
    The aim of the work was analyzing of genomic DNA of Malassezia pachydermatis isolates from clinical cases otitis externa from dogs using RAPD method with arbitrary primers Eric1R, Eric2, BG2 and FM1. Materials and methods. 47 strains of M. pachydermatis isolates from clinical cases otitis externa from dogs were tested. Isolation of genomic DNA was provided according with MasterPureTM Yeast DNA Purification Kit EPICENTRE procedure. The quality of isolated genomic DNA was determined electrophoreticaly. For differentiation the following primers were used: Eric1R, Eric2, BG2 and FM1. Primers Eric 1R and Eric 2 were used together in one reaction or amplificated separately. Obtained products were analyzed electrophoreticaly in 1.5% agarose gel. For determination of phylogenic tree Quantity one VersaDoc (BioRad) and Statgraphics plus 4.1 programs were used. Results. High degree of heterogeneity of DNA among investigated isolates of M. pachydermatis was shown using FM1 primer. Dendrograms were prepared by calculation euclid's distance of different parameters (size and count of RAPD products) by nearest neighbor method. Basing on phylogenic tree four main types (phylogenic groups) of M. pachydermatis isolates were shown. The other five groups non-count was shown also.
    Źródło:
    Annals of Parasitology; 2006, 52, 4; 299-304
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Różnicowanie izolatów Malassezia pachydermatis metodą RAPD
    RAPD differentiation of yeast like fungi Malassezia pachydermatis
    Autorzy:
    Leśniak, M.
    Dworecka-Kaszak, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/2144426.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Malassezia
    diagnostyka parazytologiczna
    grzyby
    metoda RAPD
    Malassezia pachydermatis
    parazytologia
    konferencje
    roznicowanie
    izolaty grzybowe
    Warszawa konferencja
    Opis:
    The aim of the work was analyzing of genomic DNA of Malassezia pachydermatis isolates from clinical cases otitis externa from dogs using RAPD method with arbitrary primers Eric1R, Eric2, BG2 and FM1. Materials and methods. 47 strains of M. pachydermatis isolates from clinical cases otitis externa from dogs were tested. Isolation of genomic DNA was provided according with MasterPureTM Yeast DNA Purification Kit EPICENTRE procedure. The quality of isolated genomic DNA was determined electrophoreticaly. For differentiation the following primers were used: Eric1R, Eric2, BG2 and FM1. Primers Eric 1R and Eric 2 were used together in one reaction or amplificated separately. Obtained products were analyzed electrophoreticaly in 1.5% agarose gel. For determination of phylogenic tree Quantity one VersaDoc (BioRad) and Statgraphics plus 4.1 programs were used. Results. High degree of heterogeneity of DNA among investigated isolates of M. pachydermatis was shown using FM1 primer. Dendrograms were prepared by calculation euclid's distance of different parameters (size and count of RAPD products) by nearest neighbor method. Basing on phylogenic tree four main types (phylogenic groups) of M. pachydermatis isolates were shown. The other five groups non-count was shown also.
    Źródło:
    Wiadomości Parazytologiczne; 2006, 52, 4; 299-304
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Metody roznicowania gatunkow nicieni z rodzaju Trichinella
    Methods and tools for parasite differentiation within the genus Trichinella
    Autorzy:
    Pastusiak, K
    Powiązania:
    https://bibliotekanauki.pl/articles/840918.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    izoenzymy
    hybrydyzacja kwasow nukleinowych
    roznicowanie gatunkow
    pasozyty
    nicienie
    sekwencje DNA
    Warszawa konferencja
    DNA mitochondrialny
    parazytologia
    konferencje
    Trichinella
    zroznicowanie gatunkowe
    lancuchowa reakcja polimerazy
    biologia molekularna
    znakowanie DNA
    Opis:
    This review summarizes the major biological, biochemical and molecular methods which have been developed during last 20 years to distinguish parasites of the genus Trichinella. From the time of the discovery of Trichinella in 1835 until the 1970, it was assumed that trichinellosis was caused by a single species of parasite, Trichinella spiralis. Many biological parameters have been compared to differentiate the parasite, such as host specificity, geographical distribution, reproductive abilities, nurse cell development and resistance to freezing. Now, investigators realize that the genus Trichinella is a much more complex group of parasites and simple biological methods are unsufficient. In order to identify and better characterize the species and genotypes of Trichinella it was necessary to develop more sensitive techniques. First, for detecting Trichinella infection immunological methods have been used, such as detection of antibodies in host blood and antigens of parasites using monoclonal antibodies against immunodominant proteins. Later, biochemical techniques have been used such as isoenzyme analysis. The main goal of these methods is to provide a simple, rapid and reproducible techniques to differentiate Trichinella parasites. For this purpose DNA-based methods appeared the best ones. Beginning with the use restriction enzymes, repetitive DNA probes for detection of parasite DNA, and later techniques based on the polymerase chain reaction (PCR), give results at the high level of sensitivity. All of this information has been used to construct a new taxonomy of the genus Thrichinella. To date, 11 taxa have been recognized in the genus: 8 species (Trichinella spiralis T1, Trichinella nativa T2, Trichinella britovi T3, Trichinella pseudospiralis T4, Trichinella murrelli T5, Trichinella nelsoni T7, Trichinella papuae T10, Trichinella zimbabwensis T11) and additionally three genotypes whose taxonomic status is yet uncertain (T6, T8, T9). Based upon morphology, epidemiology of trichinellosis, geographical distribution and host range of the parasite, two main groups are recognized in the genus Trichinella. The first group comprises species that encapsulate in host muscle tissue, while the species of the second group do not encapsulate. The species and genotypes of the first group infect only mammals (T. spiralis, T. nativa, T. britovi, T. murrelli, T. nelsoni, T6, T8 and T9), whereas of the three species from the second group, one parasitises mammals and birds (T. pseudospiralis) and the other two infect mammals and reptiles (T. papuae and T. zimbabwensis). Due to the big genetic differences between Trichinella isolates, investigators predict that the number of species and genotypes found within Trichinella will be increased.
    Źródło:
    Annals of Parasitology; 2006, 52, 3; 165-173
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Metody różnicowania gatunków nicieni z rodzaju Trichinella
    Methods and tools for parasite differentiation within the genus Trichinella
    Autorzy:
    Pastusiak, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/2144338.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    izoenzymy
    hybrydyzacja kwasow nukleinowych
    roznicowanie gatunkow
    pasozyty
    nicienie
    sekwencje DNA
    Warszawa konferencja
    DNA mitochondrialny
    parazytologia
    konferencje
    Trichinella
    zroznicowanie gatunkowe
    lancuchowa reakcja polimerazy
    biologia molekularna
    znakowanie DNA
    Opis:
    This review summarizes the major biological, biochemical and molecular methods which have been developed during last 20 years to distinguish parasites of the genus Trichinella. From the time of the discovery of Trichinella in 1835 until the 1970, it was assumed that trichinellosis was caused by a single species of parasite, Trichinella spiralis. Many biological parameters have been compared to differentiate the parasite, such as host specificity, geographical distribution, reproductive abilities, nurse cell development and resistance to freezing. Now, investigators realize that the genus Trichinella is a much more complex group of parasites and simple biological methods are unsufficient. In order to identify and better characterize the species and genotypes of Trichinella it was necessary to develop more sensitive techniques. First, for detecting Trichinella infection immunological methods have been used, such as detection of antibodies in host blood and antigens of parasites using monoclonal antibodies against immunodominant proteins. Later, biochemical techniques have been used such as isoenzyme analysis. The main goal of these methods is to provide a simple, rapid and reproducible techniques to differentiate Trichinella parasites. For this purpose DNA-based methods appeared the best ones. Beginning with the use restriction enzymes, repetitive DNA probes for detection of parasite DNA, and later techniques based on the polymerase chain reaction (PCR), give results at the high level of sensitivity. All of this information has been used to construct a new taxonomy of the genus Thrichinella. To date, 11 taxa have been recognized in the genus: 8 species (Trichinella spiralis T1, Trichinella nativa T2, Trichinella britovi T3, Trichinella pseudospiralis T4, Trichinella murrelli T5, Trichinella nelsoni T7, Trichinella papuae T10, Trichinella zimbabwensis T11) and additionally three genotypes whose taxonomic status is yet uncertain (T6, T8, T9). Based upon morphology, epidemiology of trichinellosis, geographical distribution and host range of the parasite, two main groups are recognized in the genus Trichinella. The first group comprises species that encapsulate in host muscle tissue, while the species of the second group do not encapsulate. The species and genotypes of the first group infect only mammals (T. spiralis, T. nativa, T. britovi, T. murrelli, T. nelsoni, T6, T8 and T9), whereas of the three species from the second group, one parasitises mammals and birds (T. pseudospiralis) and the other two infect mammals and reptiles (T. papuae and T. zimbabwensis). Due to the big genetic differences between Trichinella isolates, investigators predict that the number of species and genotypes found within Trichinella will be increased.
    Źródło:
    Wiadomości Parazytologiczne; 2006, 52, 3; 165-173
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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