Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Wyszukujesz frazę "parazytologia weterynaryjna" wg kryterium: Temat


Wyświetlanie 1-11 z 11
Tytuł:
Parazytologia na VIII Kongresie Polskiego Towarzystwa Nauk Weterynaryjnych
Parasitology at the VIII Congress off the Polish Society of Veterinary Sciences
Autorzy:
Fagasinski, A.
Powiązania:
https://bibliotekanauki.pl/articles/836208.pdf
Data publikacji:
1988
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
towarzystwa naukowe
Polskie Towarzystwo Nauk Weterynaryjnych
kongresy
Warszawa konferencja
konferencje
parazytologia
parazytologia weterynaryjna
Źródło:
Annals of Parasitology; 1988, 34, 4-6
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Podsumowanie obrad i zamknięcie XV Zjazdu Polskiego Towarzystwa Parazytologicznego
Summing up of the conferences and conclusion of the XV Congress of the Polish Parasitological Society
Autorzy:
Kasprzak, W.
Powiązania:
https://bibliotekanauki.pl/articles/837041.pdf
Data publikacji:
1988
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
towarzystwa naukowe
Polskie Towarzystwo Parazytologiczne
jubileusz
zjazdy naukowe
parazytologia
parazytologia lekarska
parazytologia weterynaryjna
konferencje
Katowice konferencja
Źródło:
Annals of Parasitology; 1988, 34, 4-6
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The 20th International Conference of the World Association for the Advancement of Veterinary Parasitology, Christchurch, New Zealand
Autorzy:
Mizgajska-Wiktor, H.
Andrzejewska, I.
Powiązania:
https://bibliotekanauki.pl/articles/840345.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
zwalczanie pasozytow
Christchurch konferencja
pasozyty zwierzat
protozoologia weterynaryjna
pasozyty zewnetrzne
konferencje
inwazja pasozytnicza
parazytologia weterynaryjna
profilaktyka weterynaryjna
zwierzeta hodowlane
Źródło:
Annals of Parasitology; 2006, 52, 2; 141-142
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Aktualne problemy włośnicy w Polsce
Contemporary problems of trichinosis in Poland
Autorzy:
Fagasinski, A.
Powiązania:
https://bibliotekanauki.pl/articles/836182.pdf
Data publikacji:
1988
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parazytologia
parazytologia lekarska
parazytologia weterynaryjna
choroby czlowieka
choroby zwierzat
choroby pasozytnicze
wlosnica
sytuacja epidemiologiczna
sytuacja epizootyczna
stan obecny
Polska
konferencje
Warszawa konferencja
Źródło:
Annals of Parasitology; 1988, 34, 2
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Aktualne problemy włośnicy w Polsce
Contemporary problems of trichinosis in Poland
Autorzy:
Fagasiński, A.
Powiązania:
https://bibliotekanauki.pl/articles/2152587.pdf
Data publikacji:
1988
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parazytologia
parazytologia lekarska
parazytologia weterynaryjna
choroby czlowieka
choroby zwierzat
choroby pasozytnicze
wlosnica
sytuacja epidemiologiczna
sytuacja epizootyczna
stan obecny
Polska
konferencje
Warszawa konferencja
Źródło:
Wiadomości Parazytologiczne; 1988, 34, 2; 219-222
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykorzystanie sekwencji genu rRNA w molekularnej diagnostyce parazytologicznej
Molecular diagnostic of parasites using rRNA gene sequence
Autorzy:
Dlugosz, E
Wisniewski, M.
Powiązania:
https://bibliotekanauki.pl/articles/841440.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
geny kodujace
diagnostyka parazytologiczna
diagnostyka weterynaryjna
wykorzystanie
parazytologia
konferencje
sekwencja genu
gen rRNA
Warszawa konferencja
Opis:
Ribosomal RNA (rRNA) is a component of the ribosomes. Eukaryotic ribosomes contain four different rRNA molecules: 18S, 5,8S, 28S and 5S rRNA. rRNA is the most conserved (least variable) gene in all cells. For this reason, genes that encode the rRNA (rDNA) are sequenced to identify an organism's taxonomic group, calculate related groups, and estimate rates of species divergence. Especially the internal transcribed spacers (ITS) are very useful for molecular diagnostic of parasite. They are noncoding regions of DNA sequence that separate genes coding for the 28S, 5.8S, and 18S ribosomal RNAs. These ribosomal RNA (rRNA) genes are highly conserved across taxa while the spacers between them may be species-specific. In this paper authors describe practical using of rRNA gene to parasite diagnostic.
Źródło:
Annals of Parasitology; 2006, 52, 4; 263-269
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykorzystanie sekwencji genu rRNA w molekularnej diagnostyce parazytologicznej
Molecular diagnostic of parasites using rRNA gene sequence
Autorzy:
Długosz, E.
Wiśniewski, M.
Powiązania:
https://bibliotekanauki.pl/articles/2144424.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
geny kodujace
diagnostyka parazytologiczna
diagnostyka weterynaryjna
wykorzystanie
parazytologia
konferencje
sekwencja genu
gen rRNA
Warszawa konferencja
Opis:
Ribosomal RNA (rRNA) is a component of the ribosomes. Eukaryotic ribosomes contain four different rRNA molecules: 18S, 5,8S, 28S and 5S rRNA. rRNA is the most conserved (least variable) gene in all cells. For this reason, genes that encode the rRNA (rDNA) are sequenced to identify an organism's taxonomic group, calculate related groups, and estimate rates of species divergence. Especially the internal transcribed spacers (ITS) are very useful for molecular diagnostic of parasite. They are noncoding regions of DNA sequence that separate genes coding for the 28S, 5.8S, and 18S ribosomal RNAs. These ribosomal RNA (rRNA) genes are highly conserved across taxa while the spacers between them may be species-specific. In this paper authors describe practical using of rRNA gene to parasite diagnostic.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 4; 263-269
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Przydatność ELISA do diagnozowania włośnicy u świń i dzików
The usefulness of ELISA for diagnosis of trichinellosis in pigs and wild boars
Autorzy:
Bień, J.
Powiązania:
https://bibliotekanauki.pl/articles/2144342.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
choroby pasozytnicze
diagnostyka weterynaryjna
Polska
walidacja
pasozyty
Warszawa konferencja
trzoda chlewna
dziki
wykorzystanie
parazytologia
konferencje
Trichinella
choroby zwierzat
test ELISA
wykrywanie
Opis:
ELISA can be used to measure produced antibodies or Trichinella spp. antigens in the samples. They are detected with antibodies linked to an enzyme that reacts with a substrate and generate a colour reaction. The optical density (OD) of the reaction is measured spectrophotometrically. ELISA assays can be done in several different procedures called „direct”, "indirect", "sandwich", and "competition" ELISA. Since the 1970s, the studies have been done on improving or replacing direct methods of Trichinella diagnosis with serological methods based on the ELISA. When somatic antigens of L1 T. spiralis were used, the specificity of the ELISA was poor due to a high probability of cross-reactions with other pathogens. During the 1980s the specificity of the ELISA was improved by excretory-secretory (E/S) antigens obtained during Trichinella muscle larvae incubation in vitro. Recently a synthetic glycan antigen has been developed and the increasing of ELISA specificity and sensitivity was noticed. The sensitivity of the ELISA using an E/S antigen ranging from 93.1 to 99.2% but the specificity from 90.6 to 99.4%. The ELISA method is relatively simple to apply, reliable, readily standardized and provides an acceptable balance of sensitivity and specificity. But all modified procedures should be validated. In Poland, the studies on the usefulness of ELISA for antibodies detection against T. spiralis in pigs and wild animals are limited. Own ELISA procedure was prepared in Pathophysiology Lab. in W. Stefański Institute of Parasitology of PAS. ELISA was used to examine IgG level against L1 T. spiralis in pigs and wild boars serum samples. Of 1474 pig samples, only 12 were positive. Of 1880 wild boar samples only 14 were positive. The results of this study are comparable with performance obtained using commercial sets. The results showed the usefulness of ELISA for T. spiralis diagnosis in pigs and wild boars and confirmed the possibility of use the ELISA test for application in the slaughterhouse.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 205-212
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ procedury otrzymywania antygenów ekskrecyjno-sekrecyjnych Trichinella spiralis na wynik testu ELISA u zarażonych świń
The influence of the procedure of excretory -secretory L1 Trichinella spiralis antigen preparation on the efficiency of an ELISA test in pigs
Autorzy:
Bień, J.
Powiązania:
https://bibliotekanauki.pl/articles/2144343.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
analiza statystyczna
diagnostyka weterynaryjna
pasozyty
nicienie
Warszawa konferencja
otrzymywanie
procedury
trzoda chlewna
zwierzeta chore
parazytologia
Trichinella spiralis
konferencje
antygeny
wartosc diagnostyczna
test ELISA
Opis:
Background. Trichinellosis is a parasitic zoonosis transmitted to humans by consumption of raw or undercooked meat from animals infected by worms of the Trichinella genus. Every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease in Poland. The usefulness of ELISA for anti-T. spiralis IgG detection in pigs is still limited by the nature of antigen. The objective in the present study was to compare the usefulness of excretory-secretory antigens of L1 T. spiralis for the serological detection of IgG antibodies in pigs. Material and methods. The antigens were prepared in different laboratories: Ag ES L1 T. spiralis (N) in Germany, Ag ES L1 T. spiralis (W) in Italy and Ag ES L1 T. spiralis in Poland. Conventional, Iberian pigs were infected with 200, 1000 and 20 000 muscle larvae of T. spiralis. Serum samples were obtained at 5 and 1 dbi (day before infection), and 5, 10, 15, 20, 25, 30, 40, 50, 60 dpi (day post infection) and screened for specific IgG antibodies to excretory-secretory L1 T. spiralis antigens. Serum samples were obtained from the EU project TRICHIPORSE. The cut-off value of ELISA was determined on serum samples from 248 Trichinella-free pigs from Poznań and Boża Wola, that were examined by artificial digestion. Results. In pigs infected with 200 L1 T. spiralis larvae, specific IgG were detectable from 50 dpi, when the Ag ES L1 T. spiralis (N) was used, whereas when Ag ES L1T. spiralis (W) and Ag ES L1 T. spiralis were used, the specific IgG were detectable from 40 dpi. In pigs infected with 1000 L1 T. spiralis larvae, specific IgG was observed from 30 dpi when Ag ES L1 T. spiralis (W) and Ag ES L1 T. spiralis were used, but when Ag ES L1 T. spiralis (N) was used specific IgG were detectable from 40 dpi. In the group infected with the highest dose of T. spiralis larvae, specific IgG were detectable from 30 dpi when Ag ES L1 T. spiralis (N) and Ag ES L1 T. spiralis (W) were used, whereas when Ag ES L1 T. spiralis was used specific IgG were detectable from 20 dpi. The results strongly indicated that in the examined pigs, the specific IgG response against T. spiralis infection is dose dependent. Furthermore, it was shown that the high infectious dose induced earlier increasing of specific IgG response. Statistical analysis revealed a significant positive correlation between OD values obtained in procedures based on the three antigens. The results were statistically repeatable for procedures and for single pigs (P<0.01).
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 219-230
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Przydatnosc ELISA do diagnozowania wlosnicy u swin i dzikow
The usefulness of ELISA for diagnosis of trichinellosis in pigs and wild boars
Autorzy:
Bien, J
Powiązania:
https://bibliotekanauki.pl/articles/840219.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
choroby pasozytnicze
diagnostyka weterynaryjna
Polska
walidacja
pasozyty
Warszawa konferencja
trzoda chlewna
dziki
wykorzystanie
parazytologia
konferencje
Trichinella
choroby zwierzat
test ELISA
wykrywanie
Opis:
ELISA can be used to measure produced antibodies or Trichinella spp. antigens in the samples. They are detected with antibodies linked to an enzyme that reacts with a substrate and generate a colour reaction. The optical density (OD) of the reaction is measured spectrophotometrically. ELISA assays can be done in several different procedures called „direct”, "indirect", "sandwich", and "competition" ELISA. Since the 1970s, the studies have been done on improving or replacing direct methods of Trichinella diagnosis with serological methods based on the ELISA. When somatic antigens of L1 T. spiralis were used, the specificity of the ELISA was poor due to a high probability of cross-reactions with other pathogens. During the 1980s the specificity of the ELISA was improved by excretory-secretory (E/S) antigens obtained during Trichinella muscle larvae incubation in vitro. Recently a synthetic glycan antigen has been developed and the increasing of ELISA specificity and sensitivity was noticed. The sensitivity of the ELISA using an E/S antigen ranging from 93.1 to 99.2% but the specificity from 90.6 to 99.4%. The ELISA method is relatively simple to apply, reliable, readily standardized and provides an acceptable balance of sensitivity and specificity. But all modified procedures should be validated. In Poland, the studies on the usefulness of ELISA for antibodies detection against T. spiralis in pigs and wild animals are limited. Own ELISA procedure was prepared in Pathophysiology Lab. in W. Stefański Institute of Parasitology of PAS. ELISA was used to examine IgG level against L1 T. spiralis in pigs and wild boars serum samples. Of 1474 pig samples, only 12 were positive. Of 1880 wild boar samples only 14 were positive. The results of this study are comparable with performance obtained using commercial sets. The results showed the usefulness of ELISA for T. spiralis diagnosis in pigs and wild boars and confirmed the possibility of use the ELISA test for application in the slaughterhouse.
Źródło:
Annals of Parasitology; 2006, 52, 3; 205-212
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wplyw procedury otrzymywania antygenow ekskrecyjno-sekrecyjnych Trichinella spiralis na wynik testu ELISA u zarazonych swin
The influence of the procedure of excretory -secretory L1 Trichinella spiralis antigen preparation on the efficiency of an ELISA test in pigs
Autorzy:
Bien, J
Powiązania:
https://bibliotekanauki.pl/articles/840241.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
analiza statystyczna
diagnostyka weterynaryjna
pasozyty
nicienie
Warszawa konferencja
otrzymywanie
procedury
trzoda chlewna
zwierzeta chore
parazytologia
Trichinella spiralis
konferencje
antygeny
wartosc diagnostyczna
test ELISA
Opis:
Background. Trichinellosis is a parasitic zoonosis transmitted to humans by consumption of raw or undercooked meat from animals infected by worms of the Trichinella genus. Every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease in Poland. The usefulness of ELISA for anti-T. spiralis IgG detection in pigs is still limited by the nature of antigen. The objective in the present study was to compare the usefulness of excretory-secretory antigens of L1 T. spiralis for the serological detection of IgG antibodies in pigs. Material and methods. The antigens were prepared in different laboratories: Ag ES L1 T. spiralis (N) in Germany, Ag ES L1 T. spiralis (W) in Italy and Ag ES L1 T. spiralis in Poland. Conventional, Iberian pigs were infected with 200, 1000 and 20 000 muscle larvae of T. spiralis. Serum samples were obtained at 5 and 1 dbi (day before infection), and 5, 10, 15, 20, 25, 30, 40, 50, 60 dpi (day post infection) and screened for specific IgG antibodies to excretory-secretory L1 T. spiralis antigens. Serum samples were obtained from the EU project TRICHIPORSE. The cut-off value of ELISA was determined on serum samples from 248 Trichinella-free pigs from Poznań and Boża Wola, that were examined by artificial digestion. Results. In pigs infected with 200 L1 T. spiralis larvae, specific IgG were detectable from 50 dpi, when the Ag ES L1 T. spiralis (N) was used, whereas when Ag ES L1T. spiralis (W) and Ag ES L1 T. spiralis were used, the specific IgG were detectable from 40 dpi. In pigs infected with 1000 L1 T. spiralis larvae, specific IgG was observed from 30 dpi when Ag ES L1 T. spiralis (W) and Ag ES L1 T. spiralis were used, but when Ag ES L1 T. spiralis (N) was used specific IgG were detectable from 40 dpi. In the group infected with the highest dose of T. spiralis larvae, specific IgG were detectable from 30 dpi when Ag ES L1 T. spiralis (N) and Ag ES L1 T. spiralis (W) were used, whereas when Ag ES L1 T. spiralis was used specific IgG were detectable from 20 dpi. The results strongly indicated that in the examined pigs, the specific IgG response against T. spiralis infection is dose dependent. Furthermore, it was shown that the high infectious dose induced earlier increasing of specific IgG response. Statistical analysis revealed a significant positive correlation between OD values obtained in procedures based on the three antigens. The results were statistically repeatable for procedures and for single pigs (P<0.01).
Źródło:
Annals of Parasitology; 2006, 52, 3; 219-230
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-11 z 11

    Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies