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    Wyświetlanie 1-4 z 4
    Tytuł:
    Antibiotic susceptibility of bacteria isolated from respiratory tract of pigs in Poland between 2004 and 2008
    Autorzy:
    Markowska-Daniel, I
    Urbaniak, K.
    Stepniewska, K.
    Pejsak, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/30384.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Polska
    pig
    respiratory tract
    bacteria
    isolation
    antibiotic susceptibility
    animal health
    productivity
    animal production
    Opis:
    Antibiotic susceptibility of bacteria isolated from nasal swabs and lungs of pigs, to 16 commonly used antibiotics, was determined by disc diffusion test, ß-lactams showed the best activity against Streptococcus suis (S. suis) (> 99% of susceptible strains). The lowest sensitivity of S. suis was evidenced to: tylosin, tetracycline and neomycin (50%, 40% and 25%, respectively). Isolates of Escherichia coli (E. coli) demonstrated the highest susceptibility to cephalosporin (85% strains), gentamicin and norfloxacin (over 74%). The lowest susceptibility of E. coli was demonstrated to tiamulin and penicillin (11.3% and 1.9%, respectively). Over 80% of Actinobacillus pleuropneumoniae (App) strains were susceptible to all antibiotics tested. The highest resistance of App, but demonstrated by below 20% of tested isolates only, was evidenced to neomycin and LxS. Isolates of Pasteurella multocida (Pm), Haemophilus parasuis (Hps) and Arcanobacterium pyogenes (A. pyogenes) were highly susceptible to the most antibiotics included in the analysis. The comparison of the in vitro susceptibility of pathogens to the chemotherapeutics used on Polish farms for the therapy of bacterial infection of pigs within the last five years and the last 10 years, showed an increasing percent of E. coli and S. suis strains resistant to commonly used antibiotics. It is also shown that Pm, Hps, App and A. pyogenes isolates were continuously susceptible to the most chemotherapeutics applied.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 1; 29-36
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular analysis of a fragment of gene E1B 19K of canine adenovirus 2 (CAV-2) isolated from dogs with symptoms of cough
    Autorzy:
    Kalinowski, M.
    Adaszek, L.
    Miloszowska, P.
    Skrzypczak, M.
    Zietek-Barszcz, A.
    Kutrzuba, J.
    Gradzki, Z.
    Winiarczyk, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/31384.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    molecular analysis
    gene
    canine adenovirus 2
    isolation
    dog
    disease symptom
    cough
    upper respiratory tract infection
    Opis:
    The aim of this study was to perform molecular analysis of canine adenovirus 2 (CAV-2) E1B 19K gene fragment isolated from 20 dogs of various breeds (12 males and 8 females aged 1-9 years), with clinical symptoms of upper respiratory tract infections, from the Lubelszczyzna region. Nasal swabs were taken from dogs. DNA of CAV-2 was detected using the PCR method in 16 swabs. All PCR products were sequenced, and the obtained sequences were compared with each other and with the sequence of the E1B 19K gene of the CAV-2 strain from an online database of NCBI GenBank: AC 000003. Based on analysis of the obtained sequences, three polymorphic variants of CAV-2 (No.1-3) with homology of 78 - 100% were distinguished. The nucleotide and amino acid sequences of the most frequently represented polymorphic variant, No. 1, differed from the sequences of polymorphic variant No. 2 with one substitution. The nucleotide and amino acid sequence of the E1B 19K gene of CAV-2 AC 000003 differed from the analogous sequences of representatives of variant No. 1 with 44 nucleotide and 19 amino acid substitutions. The small number of nucleotide differences in the E1B 19K CAV-2 gene among the examined own isolates, compared with AC 000003, suggest that the infections in dogs were caused by a relatively genetically stable virus which occurs in eastern Poland.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Susceptibility testing of Aspergillus niger strains isolated from poultry to antifungal drugs - a comparative study of the disk diffusion, broth microdilution (M 38-A) and Etest methods
    Autorzy:
    Tokarzewski, S.
    Ziolkowska, G.
    Nowakiewicz, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/32075.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    susceptibility
    Aspergillus niger
    isolation
    poultry
    antifungal drug
    disk diffusion
    broth microdilution method
    disc-diffusion method
    Etest procedure
    aspergillosis
    fungal infection
    respiratory tract
    Opis:
    The aim of this study was to determine the sensitivity of Aspergillus niger strains isolated from birds to available antifungal drugs using different in vitro assays - classical disk diffusion, Etest® and broth microdilution NCCLS/CLSI M 38-A. The study material consisted of about 2.000 swabs and samples from different species of birds. A. niger (n=10) was accounted for 6.81% of the total pool of strains isolated. Determinations were made for 13 antifungal drugs using the disk diffusion method. The A. niger exhibited high susceptibility to enilconazole, terbinafine, voriconazole, tioconazole and ketoconazole, low susceptibility to clotrimazole, miconazole and nystatin, and resistance to amphotericin B, itraconazole, pimaricin, fluconazole and 5-fluorocytosine. Minimum inhibitory concentration (MIC) was determined for 9 antifungal drugs using the micromethod of duplicate serial dilutions in a liquid medium. A. niger strains were most susceptible to enilconazole and voriconazole. MIC ranged from 0.0625 to 0.5 μg/ml for enilconazole, with MIC90-0.5 μg/ml and MIC50-0.125 μg/ml. The corresponding values for voriconazole were 0.25-1 μg/ml, 1 μg/ml and 0.5 μg/ml. MIC for amphotericin B and terbinafine ranged from 0.5 to 4 μg/ml, while the values for the remaining drugs were highly varied. MIC was measured by the gradient diffusion method using Etest® for 5 antifungal drugs: amphotericin B, fluconazole, itraconazole, ketoconazole and voriconazole. By far the highest susceptibility was obtained in the case of voriconazole, with MIC ranging from 0.0625 to 1 μg/ml. MIC for amphotericin B ranged from 0.25 to 4 μg/ml, for itraconazole and ketoconazole ranging from 0.5 to 16 μg/ml. Methods available for this purpose are not always applicable in field conditions. The present results indicate that the Etest® technique, due to its high percentage of agreement with the M 38-A microdilution method, should find application in medical and veterinary practice.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Laboratory diagnosis of mycoplasma infection in young cattle
    Autorzy:
    Gabinaitiene, A.
    Siugzdaite, J.
    Zilinskas, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/30791.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    mucous membrane
    Mycoplasma bovis
    Mycoplasma dispar
    animal infection
    antibody
    bacteriology
    cattle
    diagnosis
    histopathology
    laboratory diagnosis
    mycoplasma
    respiratory tract
    serology
    young animal
    isolation
    identification
    blood sample
    blood collecting
    Opis:
    The aim of this study was to detect Mycoplasma species in the respiratory tract of 110, 310 and 510 day-old groups of cattle by serological, bacteriological and histopathological investigations. Antibodies against M. bovis were found in 75% of the 110 day-old, in 50% – of the 310 day-old and in 55% – of the 510 day-old groups of cattle. Bacteriological examination of the samples from nasal cavities revealed that Mycoplasma carriers were found in 60% of the 110 day-old group of cattle, 40% of the 310 day-old and 40% of the 510 day-old group of cattle. Using the PCR method Mycoplasma was isolated from 25% of lung samples of the 510 day-old group of cattle. Mycoplasma bovis and Mycoplasma dispar were confirmed by serological investigations. Foci of bronchointerstitial pneumonia were determined by histopathological examination in 27.5% of lung samples. Mycoplasma bovis was isolated in 72.7% of bronchointerstitial pneumonia cases. Data processing with an SPSS 13.0 statistical package led to the conclusion that Mycoplasma bovis was found more frequently in the 110 day-old group of cattle (the youngest age group in this study) rather than in the 310 and 510 day-old groups of cattle (χ2 = 6.531; p = 0.038). The results obtained led to the conclusion that serological, bacteriological and histopathological examinations are important in detecting particular animal – carriers of Mycoplasma.
    Źródło:
    Polish Journal of Veterinary Sciences; 2011, 14, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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