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Wyszukujesz frazę "in vitro development" wg kryterium: Temat


Wyświetlanie 1-4 z 4
Tytuł:
Development of highbush blueberry (Vaccinium corymbosum hort. non L.) in vitro shoot cultures under the influence of melatonin
Rozwój pędowych kultur in vitro borówki wysokiej (Vaccinium corymbosum hort. non L.) pod wpływem melatoniny
Autorzy:
Litwińczuk, W.
Wadas-Boroń, M.
Powiązania:
https://bibliotekanauki.pl/articles/11541782.pdf
Data publikacji:
2009
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
Northern highbush blueberry
Vaccinium corymbosum
in vitro culture
melatonin
plant development
shoot culture
auxin
micropropagation
Herbert cultivar
Opis:
The influence of melatonin (MEL) in comparison with IAA, IBA (all at 5.71 µ M), and auxin-free (0) medium on development of highbush blueberry (Vaccinium corymbosum hort. non L.) ‘Herbert’ in vitro shoot cultures was examined. Depending on the kind of hormone in vitro cultures consisted of various number of axillary (AX) and adventitious (AD) shoots. The influence of melatonin on in vitro cultures was intermediate but more similar to IAA than IBA. Production of axillary shoots on media supplemented with MEL and IAA was comparable and higher than on medium with IBA. In contrast to IAA melatonin reduced development of adventitious shoots. Contrary to IBA-obtained cultures AX shoots grown on ‘IAA’, ‘0’, and ‘MEL’ media resembled AD shoots.
W pracy badano wpływ melatoniny (MEL 5.71 µ M) na rozwój pędowych kultur in vitro borówki wysokiej (Vaccinium corymbosum hort.  non L. ‘Herbert’). Odniesienie stanowiły pożywki zawierające IAA, IBA (5.71 µ M) lub wolne (0) od auksyn. W zależności od rodzaju hormonu kultury in vitro składały się z różnej liczby pędów kątowych (AX) i przybyszowych (AD). Wpływ melatoniny na kultury in vitro był pośredni, choć bardziej podobny do wpływu IAA niż IBA. Wytwarzanie pędów kątowych na pożywkach z dodatkiem melatoniny i IAA było zbliżone i większe niż na pożywce z IBA. W przeciwieństwie do IAA melatonina osłabiała rozwój pędów przybyszowych. Pędy kątowe otrzymane przy użyciu IBA wyraźnie różniły się od pędów przybyszowych. Na pozostałych pożywkach (‘IAA’, ‘0’ i ‘MEL’) wygląd pędów kątowych i przybyszowych był podobny.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2009, 08, 3; 3-12
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of aluminium and copper on the development of birch (Betula pendula Roth.) cultured in vitro and in vivo
Autorzy:
Bojarczuk, K
Szczygiel, K.
Powiązania:
https://bibliotekanauki.pl/articles/41028.pdf
Data publikacji:
2004
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
environment pollution
industrial pollution
tree
birch
Betula pendula
plant development
soil pollution
toxic metal
aluminium
copper
in vivo culture
in vitro culture
Opis:
Adventitious bud cultures were established by using buds of a selected birch clone (Betula pendula Roth.) resistant to industrial pollution. The Murashige and Skoog medium (1/2 and 1/4 MS) was used for multiplication and rooting of shoots. Aluminium was added to the medium, in the form of aluminium sulphate (50–100 mg Al dm–3), and birch culture was continued in vitro for over 12 months. The shoots developed on media with aluminium (Al+) proved to be more tolerant to aluminium and copper (added to the medium as nitrates or sulphates, at a concentration of 0.05–2.0 mM) during multiplication and rooting than those developed on media without aluminium (Al–). Rooted birch microcuttings obtained from cultures on media with aluminium (Al+) grew better in the soil from an unpolluted area (Zwierzyniec, Z) and from an area polluted by a phosphate fertilise factory (Luboń, L) than those from media without aluminium (Al–).
Źródło:
Dendrobiology; 2004, 51; 3-8
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of explants type and orientation on growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro
Autorzy:
Kozak, D.
Parzymies, M.
Świstowska, A.
Marcinek, B.
Ismael, B.S.
Powiązania:
https://bibliotekanauki.pl/articles/12663898.pdf
Data publikacji:
2019
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
plant cultivation
Brazilian jasmine
Mandevilla sanderi
ornamental plant
flowering plant
pot plant
commercial plant
plant growth
plant development
plant regeneration
explant type
multiplication
decapitation
defoliation
in vitro culture
tissue culture
cultivation experiment
Opis:
Mandevilla sanderi is an important commercial ornamental pot plant. Traditional vegetative propagation is limited due to the low rate, therefore there is a need to develop an alternative, more efficient method. There is an interest in development of micropropagation technology for the species, as it allows to obtain a lot of offsprings in a relatively short time. The aim of the present work was to estimate an influence of explants type and position on regeneration of Mandevilla sanderi in tissue culture. Four different types of explants (leafy shoot tips, decapitated leafy shoot tips, defoliated shoot tips, decapitated and defoliated shoot tips) were used in the experiment, which were placed on the media vertically, while defoliated shoot tips were placed horizontally or vertically upside down. The explants were cultivated on a Murashige and Skoog (MS) medium supplemented with 1 mg·dm–3 benzyladenine (BA) and 0.5 mg·dm–3 indole-3-butyric acid (IBA). It was noted that both explants orientation and positioning, influenced the multiplication rate. Defoliated shoot tips placed horizontally were characterized by higher multiplication rate (6.8) in comparison to upside down vertical positioning (3.2). It was also observed that removal of shoot apex improved axillary branching, while defoliation of shoots placed in a normal position reduced multiplication rate.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 111-119
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Micropropagation of Stryphnolobium japonicum
Autorzy:
Kobus, M
Kulpa, D.
Powiązania:
https://bibliotekanauki.pl/articles/41239.pdf
Data publikacji:
2009
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Styphnolobium japonicum zob.Sophora japonica
Sophora japonica
Japanese pagoda tree
pagoda tree zob.Japanese pagoda tree
scholar zob.japanese pagoda tree
Chinese scholar zob.Japanese pagoda tree
micropropagation
vegetation
development
in vitro culture
old tree
growth regulator
primary regeneration
shoot multiplication
rooting
Opis:
Observations of Japanese pagoda trees indicate that they undergo a full cycle of vegetative and generative development without self-renovation. The aim of this study was to obtain a successive media protocol propagation of Japanese pagoda tree by in vitro cultures. The effects of growth regulators were studied with reference to primary regeneration, shoot multiplication and rooting. As explants source were used the part of shoot of 90-year-oldtree. Explants were placed on MS (Murashige and Skoog 1962) basal medium with the addition of 6-benzylaminopurine – BAP (1.0–2.0 mg dm-3), thidiazuron – TDZ (0.1–0.3 mg dm-3) and indole-3-acetic acid – IAA (0.5–1.0 mg dm-3). BAP was the growth regulator which significantly increased shoot regeneration on initial explants. TDZ in turn, inhibited the formation of adventitious shoots and caused the explants which had been placed on the medium to die. The multiplication of the Japanese pagoda tree by in vitro cultures should be conducted on MS media with the addition of 0.5 mg dm-3 BAP, and they should be rooted on media with the addition of 0.3 mg dm-3 indole-3-butyric acid – IBA. It seems, that devising an efficient method of Japanese pagoda tree micropropagation is realistic.
Źródło:
Dendrobiology; 2009, 61; 23-26
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-4 z 4

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