- Tytuł:
- The choice of the anchoring protein influences the interaction of recombinant Bacillus spores with the immune system
- Autorzy:
-
Piekarska, Aurelia
Pełka, Paulina
Peszyńska-Sularz, Grażyna
Negri, Alessandro
Hinc, Krzysztof
Obuchowski, Michał
Iwanicki, Adam - Powiązania:
- https://bibliotekanauki.pl/articles/1038640.pdf
- Data publikacji:
- 2017
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
Bacillus subtilis
spore display
APCs
FliD
Clostridium difficile
immune response - Opis:
- The technology of display of heterologous proteins on the surface of Bacillus subtilis spores enables use of these structures as carriers of antigens for mucosal vaccination. Currently, there are no technical possibilities to predict whether a designed fusion will be efficiently displayed on the spore surface and how such recombinant spores will interact with cells of the immune system. In this study, we compared four variants of B. subtilis spores presenting a fragment of a FliD protein from Clostridium difficile in fusion with CotB, CotC, CotG or CotZ spore coat proteins. We show that these spores promote their own phagocytosis and activate both, the J774 macrophages and JAWSII dendritic cells of murine cell lines. Moreover, we used these spores for mucosal immunization of mice. We conclude that the observed effects vary with the type of displayed FliD-spore coat protein fusion and seem to be mostly independent of its abundance and localization in the spore coat structure.
- Źródło:
-
Acta Biochimica Polonica; 2017, 64, 2; 239-244
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł