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Wyświetlanie 1-2 z 2
Tytuł:
Crossing method for the further genetic improvement of Silybum marianum (L.) Gaertn
Autorzy:
Martinelli, T.
Powiązania:
https://bibliotekanauki.pl/articles/11871462.pdf
Data publikacji:
2018
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
plant cultivation
plant breeding
milk thistle
Silybum marianum
herbal plant
medicinal plant
plant genetics
genetic improvement
crossing
emasculation
domestication
pollination
Opis:
Silybum marianum (milk thistle) is among the top selling herbal products in the U.S., in Italy and in other countries. Despite its economic importance as an officinal and medicinal species, only few improved genotypes exist and the species is still marked by traits that are typical of non-domesticated plants. Based on this evidence, further genetic improvement is required for the complete exploitation of milk thistle as a crop species. The first step in order to allow further breeding programmes of the species is the setup of a reliable crossing protocol. The present paper, after a brief review of S. marianum flower anatomy, describes a crossing method for this plant. The correct phenological stage for emasculation is BBCH growth stage 54. If manual pollination is repeated two days after emasculation, a significant increase of F1 fruits can be obtained. By utilizing the proposed protocol, an average 70–90 florets per flower head are emasculated and percentage of success (F1 fruits harvested) usually ranges between 27 and 70%. The proposed crossing protocol will constitute an important tool in order to allow the further genetic improvement of this species.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2018, 17, 3; 101-108
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Improvement of silymarin content in cell cultures of Silybum marianum by copper sulphate elicitor
Autorzy:
Elsharnouby, M.E.
Hassan, F.A.S.
Powiązania:
https://bibliotekanauki.pl/articles/11893796.pdf
Data publikacji:
2018
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
plant cultivation
milk thistle
Silybum marianum
herbal plant
medicinal plant
cell culture
silymarin
copper sulphate
Opis:
Silybum marianum L. (Milk thistle) extracts are the main source of silymarin that is a mixture of various flavonolignan (silybin (silibinin), silydianin and silychristin). Silymarin of milk thistle has a hepatoprotective activity for liver cirrhosis and chronic inflammatory. Silybum marianum regeneration from hypocotyl explants and evaluation of their callogenesis, growth and total flavolignan (silymarin) upon copper sulphate (as abiotic elicitor) elicitation was targeted. Copper sulphate (CuSO4) was applied in concentrations of 0, 3, 5, 7 and 9 µM to elicit the silymarin production in cultures. The elicitation periods used in this study were 2, 4, 7, 14 and 28 days. Half-strength MS medium recorded better results relative to full-strength MS one and seed incubation in the darkness at room temperature resulted in rapid germination and reached to the gar lid after 10 days. Callus fresh and dry weights as well as growth index were gradually increased with increasing the copper sulphate concentration till 5 µM while decreased thereafter at any elicitation period. With the increase of the elicitation period, the increase of the previous parameters was observed. Flavonolignan (silymarin) was positively correlated with CuSO4 levels since all levels of copper sulphate significantly enhanced its content in relative to the control. Additionally, more silymarin was accumulated after 4 or 7 days and the accumulation significantly decreased when the elicitation period reached 14 days more. The highest silymarin (flavolignan) content (11.79 and 11.67 mg g–1 DW) was obtained when 5 or 7 µM copper sulphate levels were combined with 4 days elicitation period, being about five-fold of the control.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2018, 17, 2; 105-114
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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