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    Wyświetlanie 1-4 z 4
    Tytuł:
    The androgenic response in anther culture of spring wheat is greater in genotypes with solid stem in contrast to genotypes with hollow stem
    Autorzy:
    Weigt, D.
    Kiel, A.
    Nawracala, J.
    Pluta, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/951280.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    anther culture
    spring wheat
    stem
    genotype
    water-soluble carbohydrate
    androgenesis
    breeding programme
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Induced androgenesis in Phleum pratense
    Indukowana androgeneza w Phleum pratense
    Autorzy:
    Broda, Z.
    Mikołajczyk, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2234853.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Łąkarskie
    Tematy:
    induced androgenesis
    androgenesis
    Phleum pratense
    anther culture
    genotype
    timothy
    medium
    anther treatment
    homozygous line
    in vitro culture
    haploid
    microspore
    plant production
    Źródło:
    Łąkarstwo w Polsce; 2006, 09; 33-40
    1506-5162
    Pojawia się w:
    Łąkarstwo w Polsce
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cytological and genetic evaluation of anther culture-derived doubled haploids in barley
    Autorzy:
    Szarejko, I
    Falk, D E
    Janusz, A
    Nabialkowska, D
    Powiązania:
    https://bibliotekanauki.pl/articles/2046612.pdf
    Data publikacji:
    1997
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    cytological analysis
    doubled haploid
    morphological variation
    genotype
    barley
    genetic evaluation
    gametoclonal variation
    chlorophyll mutation test
    Hordeum vulgare
    anther culture
    Opis:
    Gametoclonal variation among anther culture-derived plants of three barley genotypes was estimated on the basis of cytological analysis (DH1, DH2 generation), observation of morphological variants (DH2, DH3) and chlorophyll mutation test (DH2, DH3). Individual head rows were grown in the field to detect possible chimeric structure of regenerants and to assess the number of variants and mutations in each line. Spontaneously doubled plants were the most frequent class (70%) among regenerants and almost 90% of them were completely fertile. There was a difference in proportion of haploids produced by different genotypes, but the highest frequency observed did not exceed 21%. The remaining regenerants were tetraploid, and contained chromosomal mutations or chimeras. In total, there were about 15% of polyploids and plants carrying chromosomal aberrations (translocations, inversions) among DH1 individuals. The changes in chromosome number and structure were the main source of observed variation. The level of gene mutation induced in vitro was relatively low. No more than 1% of microspore-derived plants expressed visible morphological changes in DH2 progeny. Only two morphological variants derived from the Bruce cultivar proved to be homozygous mutants (dwarf type) stable up the to third generation. The frequency of DH plants carrying chlorophyll mutation was 5.8%, but most of them (82%) were chimeric and had only a small mutation sector. The level of gametoclonal variation depended on the donor plant genotype. The highest proportion of variants and mutations was observed among DH plants derived from the Bruce cultivar, while the lowest was recorded among plants regenerated from anther culture of the doubled haploid line H930-36. Mechanisms leading to the observed variation and implications resulting from the presented experiments concerning implementation of anther culture in barley breeding were discussed. It was concluded that this method resulted in a high frequency of spontaneous doubling, a low frequency of genetic changes, and being less time and effort-consuming than the ’Bulbosum’ technique, can be applied to most barley breeding programs.
    Źródło:
    Journal of Applied Genetics; 1997, 38, 4; 437-452
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Induction of multi-nucleate microspores in anther culture of salix viminalis L.
    Autorzy:
    Wojciechowicz, M K
    Kikowska, M.A.
    Powiązania:
    https://bibliotekanauki.pl/articles/41619.pdf
    Data publikacji:
    2009
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    Salix viminalis
    genotype
    androgenesis
    thermal factor
    medium composition
    stress response
    thermal shock
    anther culture
    multinucleate microspore
    male clone
    mannitol
    Kyo medium
    microspore
    ultrastructural analysis
    initial microspore
    Opis:
    The aim of the work was to determine how the induction of androgenesis in selected genotypes of Salix viminalis is affected by thermal factors and medium composition. Anthers isolated from male clones of S. viminalis were pre-cultured at 4, 27 and 32°C for two to eight days in liquid Kyo medium with and without the addition of mannitol and on solid MS or WPM medium. The solid media were supplemented with different concentration of disaccharidesand various combinations of growth regulators including kinetin, 2iP, IAA and IBA. Multi-nucleate microspores indicative of androgenesis were observed in anthers pre-cultured for seven days at 4°C in liquid Kyo medium containing mannitol. The rate of androgenesis was higher when the anthers were transferred to solid modified MS medium containing high concentrations of sucrose and kinetin. In studied genotypes of basket willow early uninucleate microspores underwent sporophytic divisions. Ultrastructural observations showed differences in cellular arrangement of pre-stressed microspores. In Salix viminalis, microspores without starch grains and decreased number of lipid bodies were potentially androgenic cells.
    Źródło:
    Dendrobiology; 2009, 61; 55-64
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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