- Tytuł:
- Specific for DNA damages GFP microbial biosensor as a tool for genotoxic action assessment of environmental pollution
- Autorzy:
- Matejczyk, M.
- Powiązania:
- https://bibliotekanauki.pl/articles/403239.pdf
- Data publikacji:
- 2010
- Wydawca:
- Politechnika Białostocka. Oficyna Wydawnicza Politechniki Białostockiej
- Tematy:
-
uszkodzenia DNA
genotoksyczność
DNA damage
genotoxicity
recA promoter
SOS response - Opis:
- In the presented paper, autofluorescent reporter of Escherichia coli K-12 recA::gfpmut2 strain, which contained a plasmid-borne transcriptional fusion between DNA-damage inducible recA promoter involved in the SOS regulon response and fast folding GFP variant reporter gene-gfpmut2, have been used. GFP-based bacterial biosensors allowed the detection of bacterial cells response to selected tested genotoxic compounds such as mitomycin C (MMC), actinomycin D, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and formaldehyde (CH2O). Experiment indicated that E. coli K-12 recA::gfpmut2 biosensor strain is more specific and sensitive for especially two genotoxins: actinomycin D and MNNG and with very low response to other agents. So it was concluded that for formaldehyde and MMC E. coli K-12 recA::gfpmut2 genetic system is disqualified for genotoxicity screening.
- Źródło:
-
Budownictwo i Inżynieria Środowiska; 2010, 1, 4; 319-326
2081-3279 - Pojawia się w:
- Budownictwo i Inżynieria Środowiska
- Dostawca treści:
- Biblioteka Nauki
Artykuł