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Wyszukujesz frazę "flow cytometry" wg kryterium: Temat


Wyświetlanie 1-3 z 3
Tytuł:
Genome size in Humulus lupulus L. and H. japonicus Siebold and Zucc. [Cannabaceae]
Autorzy:
Grabowska-Joachimiak, A
Sliwinska, E.
Pigula, M.
Skomra, U.
Joachimiak, A.J.
Powiązania:
https://bibliotekanauki.pl/articles/59081.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
genome size
Humulus lupulus
Humulus japonicus
Cannabaceae
flow cytometry
karyotype
sex chromosome
Opis:
We analysed chromosome lengths, karyotype structure, and nuclear DNA content (flow cytometry) in diploid (2n=20) and triploid (2n=30) European H. lupulus var. lupulus, American H. lupulus var. neomexicanus (2n=20) and Japanese ornamental hop, H. japonicus (F/2n=16; M/2n=17). Diploid female representatives of H. lupulus var. lupulus and H. l. var. neomexicanus differed in total length of the basal chromosome set (23.16 µm and 25.99 µm, respectively) and nuclear 2C DNA amount (5.598 pg and 6.064 pg) but showed similar karyotype structure. No deviation from the additivity, both in chromosome length and 2C DNA amount was evidenced in triploid monoecious H. lupulus (2n=30, XXY). H. japonicus showed different karyotype structure, smaller basal chromosome set (F/18.04 µm, M/20.66 µm) and lower nuclear DNA amount (F/3.208 pg and M/3.522 pg). There are first evaluations of nuclear genome size in diploid, not commercial representative of European H. lupulus var. lupulus and American H. lupulus var. neomexicanus and first attempt to determine the absolute male and female genome size in two Humulus species.
Źródło:
Acta Societatis Botanicorum Poloniae; 2006, 75, 3; 207-214
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative cytogenetic analysis of diploid and hexaploid Chenopodium album Agg.
Autorzy:
Kolano, B
Siwinska, D.
Maluszynska, J.
Powiązania:
https://bibliotekanauki.pl/articles/56512.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
cytogenetic analysis
diploid
hexaploid
Chenopodium album
genome size
rDNA sequence
flow cytometry
polysomaty
Opis:
Two cytotypes of Chenopodium album, diploid (2n=2x=18) and hexaploid (2n=6x=54), were analysed using flow cytometry and a FISH experiment. The genome size was indicated as 1.795 pg for the diploid and 3.845 pg for the hexaploid plants which suggested genome downsizing in the evolution of hexaploid cytotype. Double FISH with 25S rDNA and 5S rDNA allowed three to five homologue chromosome pairs to be distinguished depending on the cytotype. The Variation in size and number of rDNA sites between the polyploid C. album and its putative diploid ancestor indicated that rDNA loci underwent rearrangements after polyploidization. Flow cytometry measurements of the relative nuclear DNA content in the somatic tissue of C. album revealed extensive endopolyploidization resulting in tissues comprising a mixture of cells with a different DNA content (from 2C to 32C) in varying proportions. The pattern of endopolyploidy was characteristic for the developmental stage of the plant and for the individual organ. Polysomaty was not observed in the embryo tissues however endopolyploidization had taken place in most tested organs of seedlings. The endopolyploidy in diploid and hexaploid C. album was compared to find any relationship between the pattern of polysomaty and polyploidy level in this species. This revealed that polyploid plants showed a decline in the number of endocycles as well as in the frequency of endopolyploidy cells compared to diploid plants.
Źródło:
Acta Societatis Botanicorum Poloniae; 2008, 77, 4; 293-298
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genome size and phenotypic variation of Nymphaea (Nymphaeaceae) species from Eastern Europe and temperate Asia
Autorzy:
Dabrowska, M.A.
Rola, K.
Volkova, P.
Suda, J.
Zalewska-Galosz, J.
Powiązania:
https://bibliotekanauki.pl/articles/59355.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
genome size
phenotypic variation
Nymphaea alba
Nymphaea candida
Nymphaea tetragona
Nymphaea x borealis
Nymphaeaceae
morphometric analysis
flow cytometry
interspecies hybridization
Eastern Europe
temperate region
Asia
Opis:
Despite long-term research, the aquatic genus Nymphaea still possesses major taxonomie challenges. High phenotypic plasticity and possible interspecific hybridization often make it impossible to identify individual specimens. The main aim of this study was to assess phenotypic variation in Nymphaea taxa sampled over a wide area of Eastern Europe and temperate Asia. Samples were identified based on species-specific genome sizes and diagnostic morphological characters for each taxon were then selected. A total of 353 specimens from 32 populations in Poland, Russia and Ukraine were studied, with nine biometric traits being examined. Although some specimens morphologically matched N. xborealis (a hybrid between N. alba and N. Candida) according to published determination keys, only one hybrid individual was revealed based on genome size data. Other specimens with intermediate morphology possessed genome size corresponding to N. alba, N. Candida or N. tetragona. This indicates that natural hybridization between N. alba and N. Candida is not as frequent as previously suggested. Our results also revealed a considerably higher variation in the studied morphological traits (especially the quantitative ones) in N. alba and N. Candida than reported in the literature. A determination key for the investigated Nymphaea species is provided, based on taxonomically-informative morphological characters identified in our study.
Źródło:
Acta Societatis Botanicorum Poloniae; 2015, 84, 2
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-3 z 3

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