Temat: random variation - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Genetic variation in mutants of chilli (Capsicum annuum) revealed by RAPD marker
Autorzy:: Mullainathan, L.
Sridevi, A.
Umavathi, S.
Sanjai Gandhi, E.
Powiązania:: https://bibliotekanauki.pl/articles/11592.pdf
Data publikacji:: 2014
Wydawca:: Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:: genetic variation
mutant
chilli
Capsicum annuum
random amplified polymorphic DNA analysis
RAPD marker
spice
Opis:: The present study was under taken in order to analyze the chemical mutagenesis on Chilli germplasm. In this regard, K1 variety of chilli was subjected to different mutagenic concentration for inducing mutagenesis. The M3 plants exposed to EMS and DES to produce clear difference from the untreated control, thus indicating that mutagenic treatment produce polymorphic regions in the chilli. For extraction of genomic DNA was adopted an improved protocol of CTAB method with slight modification. A total of ten primers were used to screen the polymorphism among the treated populations line tall, tall with chlorophyll deficient, leaf, flower, GMS and DNA damages in maturity mutants were analyzed with control. Out of ten primers, four primers (PGF02, PGF03, PGF04 AND OP107) were successfully amplified in all the samples used for this study. The successful primers were amplified in to 93 products showing an average of 9.3 bands.
Źródło:: International Letters of Natural Sciences; 2014, 06
2300-9675
Pojawia się w:: International Letters of Natural Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Weed infestation of a winter wheat canopy under the conditions of application of different herbicide doses and foliar fertilization
Zachwaszczenie łanu pszenicy ozimej w warunkach stosowania zróżnicowanych dawek herbicydów oraz nawożenia dolistnego
Autorzy:: Kraska, P.
Okon, S.
Palys, E.
Powiązania:: https://bibliotekanauki.pl/articles/27330.pdf
Data publikacji:: 2009
Wydawca:: Polskie Towarzystwo Botaniczne
Tematy:: weed infestation
winter wheat
wheat
canopy
plant condition
application
herbicide dose
foliar fertilization
DNA analysis
genetic variation
random amplified polymorphic DNA
Opis:: The present study was carried out in the years 2006– 2008 in the Bezek Experimental Farm (University of Life Sciences, Lublin). A two-factor field experiment was set up according to a randomized block design, in three replications. The experimental field was situated on medium heavy mixed rendzina developed from chalk rock with medium dusty loam granulometric composition. The soil was characterised by neutral pH, a very high content of P (342.1) and K (278.9) along with a very low level of magnesium (16.0 mg kg-1 of soil) and organic carbon (over 3.5%). The aim of this research was to compare the effect of three herbicide doses and two foliar fertilizers applied in a winter wheat canopy on weed infestation. The herbicides Mustang 306 SE 0.4 l ha-1 and Attribut 70 WG 60 g ha-1 were applied at full recommended doses as well as at doses reduced to 75% and 50%. Foliar fertilizers Insol 3 (1 1 ha-1) and FoliCare (20 kg ha-1) were applied at full recommended doses twice in the growing season BBCH* development stage 23-25* and 33-35*). The control was not treated with the herbicides and foliar fertilizers. The weed infestation level was determined by means of the quantitative gravimetric method at two dates: the first one 6 weeks after herbicide application and the second one – before harvest. The number of weed individuals was counted; species composition and air-dry biomass of aboveground parts were estimated from randomly selected areas of 1 m 0.25 m at four sites on each plot. Galium aparine and Apera spica-venti plants were sampled for molecular analysis 6 weeks after herbicide application (the treatments with the full herbicide dose, a 50% dose and the control without herbicides). The density of weeds and weed air-dry weight were statistically analysed by means of variance analysis, and the mean values were estimated with Tukey’s confidence intervals (p=0.05). It was found that the number of weeds and air-dry weight of weeds in the control treatment were significantly higher in comparison with the herbicide treated plots. The application of different herbicide doses did not differentiate significantly the weed infestation level in the winter wheat canopy. Galium aparine, Papaver rhoeas, Viola arvensis and Apera spica-ventiwere dominant weed species in the winter wheat canopy. Foliar application of fertilizers did not influence the weed infestation level in the crop canopy. Molecular analysis showed that herbicide application did not affect genetic variation in the populations of Galium aparine and Apera spica-venti.
Badania przeprowadzono w latach 2006-2008 w Gospodarstwie Doświadczalnym Bezek niedaleko Chełma. W dwuczynnikowym doświadczeniu przeprowadzonym w układzie bloków losowanych porównywano działanie trzech dawek herbicydów oraz dwóch nawozów dolistnych w łanie pszenicy ozimej odmiany Turnia uprawianej w monokulturze. Herbicydy były stosowane w pełnych zalecanych dawkach, zredukowanych do 75% i do 50%. Nawozy dolistne Insol 3 (N-11,5%; Mg-2,84%; B-0,28%; Cu-0,56%; Fe-1,20%; Mn-1,68%; Mo-0,01%; Zn-1,12%) i FoliCare (N-18,0%; P-18,1%; K-18,0%; Mg-1,5%; S-7,2%; B-0,02%; Cu-0,10%; Fe-0,20%; Mn-0,10; Mo-0,01%; Zn-0,02%) stosowano dwukrotnie w okresie wegetacji. Kontrolę stanowiły poletka na których nie stosowano zarówno herbicydów jak i nawozów dolistnych. W pracy oceniono poziom zachwaszczenia łanu (liczba osobników, skład gatunkowy i powietrznie sucha masa) pszenicy ozimej w 6 tygodni po zastosowaniu herbicydów Mustang 306 SE (florasulam – 6,25gl-1; 2,4-D EHE – 300gl-1) i Attribut 70 WG (70% propoksykarbazonu sodowego) oraz przed zbiorem. Jednocześnie podjęto próbę sprawdzenia czy wielkość dawki herbicydu może wpływać na zmiany DNA gatunków dominujących w łanie pszenicy ozimej – Galium aparine i Apera spica-venti. Poziom zachwaszczenia łanu pszenicy ozimej mierzony zarówno liczbą chwastów, jak i powietrznie suchą masą nie był istotnie różnicowany przez zastosowane dawki herbicydów. Uzyskane wyniki wskazują na możliwość obniżenia dawek herbicydów w łanie pszenicy ozimej bez ryzyka wzrostu poziomu zachwaszczenia. Nawożenie dolistne nie zmieniało poziomu zachwaszczenia łanu. Gatunkami dominującymi w łanie pszenicy ozimej w 6 tygodni po zastosowaniu herbicydów oraz przed zbiorem były Galium aparine, Papaver rhoeas oraz Viola arvensis, natomiast z jednoliściennych Apera spica-venti. Analiza molekularna nie wykazała, aby zastosowane dawki herbicydów wpłynęły na zróżnicowanie genetyczne Galium aparine i Apera spica-venti.
Źródło:: Acta Agrobotanica; 2009, 62, 2
0065-0951
2300-357X
Pojawia się w:: Acta Agrobotanica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Use of random amplified polymorphic DNA [RAPD] assay for differentiation among isolates of Stagonospora spp. and Septoria tritici
Autorzy:: Czembor, P C
Arseniuk, E
Powiązania:: https://bibliotekanauki.pl/articles/2047271.pdf
Data publikacji:: 1996
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Stagonospora
Stagonospora avenae
Septoria tritici
random amplified polymorphic DNA
pathogen
polymerase chain reaction
DNA
molecular marker
fungal isolate
Stagonospora nodorum
genetic variation
Opis:: The genetic similarity of three species: Septoria tritici, Stagonospora nodorum and Stagonospora avenae f. sp. triticea - important pathogens in many cereal production areas worldwide was assessed by random amplified polymorphic DNA (RAPD) assay. In preliminary research DNA of 14, 9, and 7 monopyenidios- pore isolates of S. nodorum, S. tritici, and S. a. tritícea, respectively, were amplified by PCR with four primers. Afterwards the research was focused on three mono- pyenidiospore isolates from each species studied. The isolates of each species selected for the study varied in pathogenicity and were diverse geographically. PCR with the set of 14 selected primers resulted in 99 different bands, ranged from 180 to 2500 base pairs in length. Most primers in PCR (especially RAD11, RAD31, RAD32, RAD33) revealed uniform bands for isolates of S. a. tritícea, that allow to identify this species among the others. The cluster analysis using Unweighed Pair-Group Method with Averaging (UPGMA) revealed interspecies disagreement among the isolates ranging from 32 to 53%. The intraspecies disagreement ranges were 17-20%, 38-43%, 42-44% for S. avenae f. sp. triticea, S. nodorum and S. tritici, respectively. Cluster analysis classified isolates into three homogeneous clusters. Each cluster grouped isolates of one species according to their current taxonomie ranks based on spore size, colony morphology and host ranges. In addition, two of the clusters represented by isolates of S. nodorum and S. a. tritícea were distinctly separated at a lower linkage distance from the third one comprising isolates of S. tritici. A slight inconsistency found in grouping some isolates indicates that such groupings should be done with caution. The present study indicates that the PCR- RAPD assay is of a potential use in taxonomy of Stagonospora spp. and Septoria tritici as well as in molecular identification of casual disease agents.
Źródło:: Journal of Applied Genetics; 1996, 37, 3; 239-251
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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