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    Wyświetlanie 1-9 z 9
    Tytuł:
    MOLECULAR DIVERSITY AND PHYLOGENY OF TRITICUM-AEGILOPS SPECIES POSSESSING D GENOME REVEALED BY SSR AND ISSR MARKERS
    Autorzy:
    Moradkhani, Hoda
    Mehrabi, Ali Ashraf
    Etminan, Alireza
    Pour-Aboughadareh, Alireza
    Powiązania:
    https://bibliotekanauki.pl/articles/2199633.pdf
    Data publikacji:
    2015-06-20
    Wydawca:
    Instytut Hodowli i Aklimatyzacji Roślin
    Tematy:
    Aegilops
    genetic diversity
    ISSR
    molecular phylogeny
    Triticum
    SSR
    Opis:
    The aim of this study is investigation the applicability of SSR and ISSR markers in evaluating the genetic relationships in twenty accessions of Aegilops and Triticum species with D genome in different ploidy levels. Totally, 119 bands and 46 alleles were detected using ten primers for ISSR and SSR markers, respectively. Polymorphism Information Content values for all primers ranged from 0.345 to 0.375 with an average of 0.367 for SSR, and varied from 0.29 to 0.44 with the average 0.37 for ISSR marker. Analysis of molecular variance (AMOVA) revealed that 81% (ISSR) and 84% (SSR) of variability was partitioned among individu-als within populations. Comparing the genetic diversity of Aegilops and Triticum accessions, based on genetic parameters, shows that genetic variation of Ae. crassa and Ae. tauschii species are higher than other species, especially in terms of Nei’s gene diversity. Cluster analysis, based on both markers, separated total accessions in three groups. However, classification based on SSR marker data was not conformed to classification ac-cording to ISSR marker data. Principal co-ordinate analysis (PCoA) for SSR and ISSR data showed that, the first two components clarified 53.48% and 49.91% of the total variation, respectively. This analysis (PCoA), also, indicated consistent patterns of genetic relationships for ISSR data sets, however, the grouping of acces-sions was not completely accorded to their own geographical origins. Consequently, a high level of genetic diversity was revealed from the accessions sampled from different eco-geographical regions of Iran.
    Źródło:
    Plant Breeding and Seed Science; 2015, 71; 81-95
    1429-3862
    2083-599X
    Pojawia się w:
    Plant Breeding and Seed Science
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Viability and genetic diversity of Populus nigra population from riparian forest in SNR Gornje Podunavlje
    Autorzy:
    Čortan, Dijana
    Tubić, Bojan
    Powiązania:
    https://bibliotekanauki.pl/articles/956812.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    black poplar
    viability assessment
    genetic diversity
    ssr markers
    snr upper danube
    Opis:
    Populus nigra L. is one of the rarest and most endangered tree species in Western and Central Europe. Its genetic diversity is of great importance in enabling a native riparian population to survive and reproduce under changing environmental conditions. The aim of this research was assessment of P. nigra viability in one of the best preserved riparian ecosystems in Europe, Special Nature Reserve “Gornje Podunavlje” (Upper Danube), Serbia. Additionally, the analysis of the genetic diversity was made to support the effective conservation in the future. During our study, we have mapped 931 P. nigra trees, which were used for the assessment of present native population. Furthermore, we used 14 microsatellite markers to assess the genetic structure of this this population. Viability assessment showed considerable occurrence of P. nigra in the research area, even though the results show fragmentation. P. nigra occurs mostly individually or in small groups of trees, and has a non-sustainable age structure due to insufficient or lacking regeneration. Despite the limited size of the studied population, the apparent overall genetic diversity was high (He = 0.759) and comparable to other known native populations of P. nigra along the Danube basin. However, the results also confirmed existence of recent bottleneck effect. Significantly positive and quite high Fis value (0.147) was noted, which may be ascribed to the “Wahlund effect” because of the population substructure that was revealed by the STRUCTURE analysis (K=2). Although results say that coverage of native stands is not so promising, most of selected trees within our research assessed showed good viability with potential for natural reproduction However, the problem is that suitable areas for natural seedling establishment are scarce and with that gene flow is probably limited. The fragmentation of the area must be reduced and isolated stands must be interlinked as there is need to create larger non-fragmented areas.
    Źródło:
    Dendrobiology; 2017, 78; 157-167
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Using SSR markers for assessment genetic diversity and detection drought escape candidate genes in barley lines (Hordeum vulgare L.)
    Autorzy:
    Gougerdchi, Vahideh
    Dezhsetan, Sara
    Ebrahimi, Mohammad Ali
    Sadeghzadeh, Behzad
    Savari, Sona
    Powiązania:
    https://bibliotekanauki.pl/articles/2199609.pdf
    Data publikacji:
    2014-12-18
    Wydawca:
    Instytut Hodowli i Aklimatyzacji Roślin
    Tematy:
    Association analysis
    barley
    genetic diversity
    Microsatellite markers (SSR)
    drought escape
    Opis:
    Assessment of genetic diversity using molecular markers is one of the primary and important steps in breeding programs. In this study, genetic diversity of 52 barley lines evaluated using 68 SSR primer pairs and 47 primer pairs produced clear and polymorphic banding pattern. In general, 153 polymorphic alleles de-tected. The number of observed polymorphic alleles varied from 2 to 9, with an average of 3.26 alleles per locus. Polymorphic Information Content (PIC) ranged from 0.07 to 0.81, with an average of 0.45. In this research, SSR markers differentiated the studied lines efficiently. Using cluster analysis, studied barley lines divided into two groups. Genetic diversity was relatively corresponding with geographical origins, because the lines related to a country somewhat diverged from each other. Two-rowed Iranian and Chinese barleys classified in one subgroup. Also, most six-rowed barleys classified in one subgroup. Association mapping analysis was used to identify candidate genes for drought escape in barley lines and 16 informative markers were identified after which confirmation in other tests could be suitable for marker assisted breeding drought escape.
    Źródło:
    Plant Breeding and Seed Science; 2014, 70; 3-14
    1429-3862
    2083-599X
    Pojawia się w:
    Plant Breeding and Seed Science
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Analysis of genetic diversity of Ficus carica L. (Moraceae) collection using simple sequence repeat (SSR) markers
    Autorzy:
    Marcotuli, I.
    Mazzeo, A.
    Nigro, D.
    Giove, L.
    Giancaspro, A.
    Colasuonno, P.
    Prgomet, Ž.
    Prgomet, I.
    Tarantino, A.
    Ferrara, G.
    Gadaleta, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/12664546.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    Moraceae
    common fig
    Ficus carica
    population structure
    genetic analysis
    genetic diversity
    SSR marker
    Opis:
    Modern technologies and accurate information on genetic diversity and structure are contributing to improve the plant breeding, in particular for all the minor species with a lack of data. Genetic diversity of 139 different Ficus carica L. genotypes collected from Italy and Croatia, and divided into two subgroups: uniferous (only main crop) and biferous (breba and main crop), was investigated using 49 microsatellite markers. A total of 70 alleles were generated, of which 64 (91.4%) showed a polymorphic pattern indicating high level of genetic diversity within the studied collection. The mean heterozygosity over the 64 single locus microsatellites was 0.33 and the expected and observed averaged variance were 16.50 and 184.08, respectively. The 139 fig genotypes formed two clusters in the PCoA analysis, suggesting a division between Italian and Croatian genotypes. Moreover, the fig accessions could be divided into two main clusters based on the STRUCTURE analysis according to the biological type, uniferous or biferous, with partly overlapping varieties. In conclusion, our results demonstrated that molecular markers were able to discriminate among genotypes and useful for the authentication of fig tree varieties (homonymies and synonymies).
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 93-109
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic diversity of Brassica rapa germplasm of Khyber Pakhtunkhwa Pakistan revealed by molecular markers
    Autorzy:
    Ali, N.
    Ali, S.
    Khan, N.U.
    Jan, S.A.
    Rabbani, M.A.
    Hussain, I.
    Powiązania:
    https://bibliotekanauki.pl/articles/12690092.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    Pakistan
    plant breeding
    Brassica rapa
    germplasm
    genetic diversity
    plant genotype
    molecular marker
    SSR marker
    Opis:
    A total of 96 indigenous Brassica rapa accessions were collected from different locations of Khyber Pakhtunkhwa, Pakistan. Simple Sequence Repeats (SSR) markers were used to identify the most diverse genotypes among the collected lots. Twenty six (26) different SSR primers were used for (genetic) variability among collected genotypes. These primers were selected from literature based on their previous results. These primers produced 135 scorable bands of which 75 were polymorphic, with an average of 55.5% polymorphic loci, and reflected the broader genetic background of the collected genotypes. An average 2.88 polymorphic bands with an average PIC value of 0.49 was recorded. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) divided all genotypes into three main groups. Group one contained three clusters, while group two and three had four and two clusters each. Based on the UPGMA dendrogram, genotypes collected from Kohat, Bannu, Swat and Haripur showed considerable amount of variation. From the present study, it is concluded that SSR markers can be proved as the best tool for the genetic variability of other local and exotic B. rapa genotypes.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 6; 57-65
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Charakterystyka wybranych markerów molekularnych
    Characteristic of selected molecular markers
    Autorzy:
    Bolc, Paulina
    Powiązania:
    https://bibliotekanauki.pl/articles/2199379.pdf
    Data publikacji:
    2020-10-22
    Wydawca:
    Instytut Hodowli i Aklimatyzacji Roślin
    Tematy:
    markery molekularne
    RFLP
    AFLP
    RAPD
    SSR
    ISSR
    SRAP
    SNP
    PCR
    polimorfizm
    różnorodność genetyczna
    molecular markers
    polymorphism
    genetic diversity
    Opis:
    Postęp, jaki nastąpił w biologii molekularnej poprzez wprowadzenie markerów molekularnych nowej generacji, w ciągu ostatnich 20 lat umożliwił znaczny rozwój wielu dziedzin badań. Możliwe stało się uzyskanie dokładniejszych informacji genetycznych pozwalających na lepsze zrozumienie zasobów genetycznych organizmów. Markerem molekularnym może być każda sekwencja nukleotydowa (wybrany fragment DNA), rozproszony w całym genomie, której zmienność między osobnikami lub grupami taksonomicznymi umożliwia precyzyjną identyfikację osobnika/taksonu. Kompilacja właściwości enzymów restrykcyjnych jak również reakcji łańcuchowej polimerazy (PCR) w technikach generujących markery molekularne pozwoliła na efektywne wykorzystanie ich w taksonomicznych, ewolucyjnych i ekologicznych badaniach roślin.
    Over the last 20 years, the progress in molecular biology through the introduction of new generation molecular markers has allowed many areas to move forward. It has now become possible to obtain more accurate genetic information to better understand the genetic resources of organisms. A molecular marker can be any nucleotide sequence (selected DNA fragment) scattered throughout the genome, whose variability between individuals or taxonomic groups allows precise identification of the individual/taxon. The effectiveness of restriction digestion and polymerase chain reaction based on molecular markers has already proved their usefulness in taxonomic, evolutionary and ecological plant research.
    Źródło:
    Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2020, 290; 27-32
    0373-7837
    2657-8913
    Pojawia się w:
    Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic diversity and relationship of Hunan province of China local tree peonies based on SSR markers
    Autorzy:
    Zhang, M.-H.
    Jin, X.-L.
    Wen, Y.-F.
    Shen, S.
    Wen-Xing
    Wu, S.
    Lu, J.-H.
    Ye-Ye
    Powiązania:
    https://bibliotekanauki.pl/articles/12665369.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    China
    Hunan Province
    plant cultivation
    tree peony
    Paeonia suffruticosa
    ornamental plant
    genetic diversity
    genetic relationship
    SSR marker
    Opis:
    Paeonia sect. Moutan is a wide known ornamental plant in the world. The objective of this study was to provide the theoretical basis for scientific preservation and utilization of tree peony resources of Hunan province of China. Simple sequence repeat (SSR) markers were applied to reveal the genetic diversity and relationship of 21 tree peony resources and 45 domestic and foreign tree peony cultivars. Clear bands, the size of which ranged from 115 bp to 379 bp, were detected with 14 primers. In total, 90 alleles were detected and the number of alleles detected with one primer varied between 5 and 13; the number of effective alleles ranged from 1.183 to 2.070; the polymorphism ratio of each locus was 100%. The observed heterozygosity, which ranged from 0.120 to 0.851 with an average of 0.532, was larger than the expected one, which ranged from 0.090 to 0.470 with an average of 0.300. Shannon index ranged from 0.137 to 0.695 and fixation index ranged from −0.332 to −0.869. The results show abundant genetic diversity in tree peony of Hunan province and SSR markers distinguishing homonymous tree peony resources successfully.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 213-223
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Porównanie zmienności genetycznej pokolenia matecznego i sztucznie wyhodowanego potomstwa sosny zwyczajnej na podstawie analiz DNA
    Comparison of the genetic variability of Scots pine trees and their progeny from nursery production based on DNA analyses
    Autorzy:
    Konecka, A.
    Tereba, A.
    Bieniek, J.
    Nowakowska, J.A.
    Powiązania:
    https://bibliotekanauki.pl/articles/985843.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    hodowla lasu
    sosna zwyczajna
    Pinus sylvestris
    zmiennosc genetyczna
    analiza DNA
    markery mikrosatelitarne
    drzewa mateczne
    drzewa potomne
    sadzonki z zakrytym systemem korzeniowym
    genetic diversity
    ssr markers
    forest nursery production
    pinus sylvestris l.
    Opis:
    The production of forest tree species in forest nurseries is performed via two main breeding systems: i) the traditional (conventional) way with the seedlings grown in soil, and ii) plants cultivated in the containers. The aim of the study was to assess the level of genetic variability in the populations of the mother stands and the progeny populations of Scots pine cultured with traditional way (in soil) and in containers in two nurseries in Olsztynek (N Poland) and Oleszyce (S Poland) forest districts. Four polymorphic microsatellite markers (SPAG 7.14, SPAC 11.6, SPAC 12.5 and SsrPt_ctg4363) were used to evaluate the genetic variability of the studied populations. The basic hypothesis assumed that higher gene pool characterizes the seedlings grown in the containers comparing to the seedlings grown in the ground. The results confirmed that. Seedlings from containerized breeding had larger gene pool and were more diverse than plants with conventional breeding, both in Olsztynek and Oleszyce. Our study revealed a significant human impact on shaping the pool of forest genetic resources of Polish forests at the early stage of nursery production and showed the need for a broader study on further stages of cultivation of forests.
    Źródło:
    Sylwan; 2018, 162, 01; 32-40
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Stan zdrowotny a zróżnicowanie genetyczne wybranych drzewostanów świerkowych na terenie RDLP w Krośnie
    Genetic variability and health of Norway spruce stands in the Regional Directorate of the State Forests in Krosno
    Autorzy:
    Gutkowska, J.
    Borys, M.
    Tereba, A.
    Tkaczyk, M.
    Oszako, T.
    Nowakowska, J.A.
    Powiązania:
    https://bibliotekanauki.pl/articles/1293265.pdf
    Data publikacji:
    2017
    Wydawca:
    Instytut Badawczy Leśnictwa
    Tematy:
    lesnictwo
    RDLP Krosno
    drzewostany swierkowe
    stan zdrowotny lasu
    drzewa lesne
    swierk pospolity
    Picea abies
    zroznicowanie genetyczne
    markery molekularne
    markery mikrosatelitarne
    DNA mitochondrialny
    Norway spruce
    health state
    SSR markers
    mtDNA
    genetic diversity
    Opis:
    The study was conducted in 2015 in six spruce stands situated in different forest districts administratively belonging to the Regional Directorate of Forests State in Krosno. Each spruce population was represented by 30 trees and assessed in terms of their current health status. Genetic analyses were performed based on shoot samples from each tree using nine nuclear DNA markers and one mitochondrial DNA marker (nad1). The health status of the trees was described according to the classification developed by Szczepkowski and Tarasiuk (2005) and the correlation between health classes and the level of genetic variability was computed with STATISTICA (α = 0.05). Nuclear DNA analyses revealed a low level of genetic variability among spruce populations (only 3% of the total genetic variation (FST = 0.028) and a high variability within populations (97%). The total heterozygosity in all stands (HT) was calculated as 0.646. Based on UPGMA analysis, the most genetically similar populations are spruce stands in the Bieszczady National Park and the Ustrzyki Dolne Forest District, which have the smallest genetic divergence of all populations (DN = 0.0165). Our analysis of the mitochondrial gene nad1 revealed the presence of six different haplotypes “a”, “a1”, “b”, “c”, “d” and “d1”. Comprising 56% of all haplotypes, “a” was the most common showing a predominant impact on spruce migration from the Carpathian area. The analysis based on mitochondrial markers (by Nei) revealed a heterozygosity of 0.525. Based on the observations of disease symptoms, 29% of the trees belong to health class 1,30% to class 2,28% to class 3 and class 4 contains 13% of trees. The comparison between health status and the level of genetic variation in the analyzed stands showed a positive correlation. Spruce stands with better health were also characterized by a greater degree of genetic variability. Since most of the investigated spruce populations shared the mitochondrial haplotype “a”, we have ascertained their Hercynian- Carpathian origin. Only one stand (Cisna) had a high frequency (43.3%) of the Nordic haplotype “c” suggesting that this provenance is derived from the Baltic post-glacial refugium of P. abies in Europe.
    Źródło:
    Leśne Prace Badawcze; 2017, 78, 1
    1732-9442
    2082-8926
    Pojawia się w:
    Leśne Prace Badawcze
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-9 z 9

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