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Wyświetlanie 1-2 z 2
Tytuł:
Struktura zbiorowisk grzybów mykoryzowych po pożarze drzewostanu sosny zwyczajnej na siedlisku boru suchego
Structure of post-fire ectomycorrhizal communities of Scots pine stand in a dry coniferous forest habitat
Autorzy:
Hilszczańska, D.
Gil, W.
Olszowska, G.
Powiązania:
https://bibliotekanauki.pl/articles/980282.pdf
Data publikacji:
2019
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
siedlisko boru suchego
drzewostany sosnowe
pozary lasow
tereny popozarowe
sosna zwyczajna
Pinus sylvestris
mikoryza
grzyby mikoryzowe
zbiorowiska grzybow
struktura zbiorowisk
roznorodnosc gatunkowa
forest fire
mycorrhiza
fungi
scots pine
coniferous forest habitat
Opis:
Ectomycorrhizal communities associated with Pinus sylvestris growing on dry coniferous forest habitat after the fire were studied. In order to investigate the fire effect on ectomycorrhizal fungi and changes of soil parameters in the upper (0−15 cm) soil layer three study plots were established: 1 – the control, 2 – fire zone left for artificial renewal (with the economic method of renewal) and 3 – fire zone left for natural renewal. The analysis of mycorrhizae revealed presence of eight mycorrhizal fungi on Scots pine roots and the value of Shannon−Wiener species diversity index H’ equaled to 1.76. The most abundant were mycorrhizae formed by Tomentella feruginea (31.7%) and Hebeloma crustuliniforme (23.3%). The fraction of Paxillus involutus and Rhizopogon sp. mycorrhizae was 16.7% and 13.0%, respectively. The lowest was the number of Thelephora terrestris mycorrhizae (only 1.7%). At the site 2, number of mycorrhizal fungi diminished to five and Shannon−Wiener species diversity index was also lower (1.37). The predominant were mycorrhizae of Cenococcum geophilum (26.7%) and P. involutus (21.7%), whilst the lowest were mycorrhizae of Suillus luteus (1.7%). Although at the third site the number of ectomycorrhizal fungi was the same as on the site 2, the dominance of Thelephora terrestris (66.7%) caused that Shannon−Wiener’s species diversity index was the lowest (1.05). Mycorrhizae of Paxillus involutus and Wilcoxina mikolae were characterised by similar abundance (13.3% and 11.7%, respectively). The lowest number of mycorrhizae was observed for Tomentella sp. (3.3%). Analysis of soil parameters showed an increase of pH on burnt sites in comparison to the control. The soil of burnt sites were also characterised by lower value of C and C/N ratio than the soil on the control treatment. The result showed that ubiquitous mycorrhizal fungi, such as T. terrestis, are able to persist on roots in changed soil environment with low content of nutritions.
Źródło:
Sylwan; 2019, 163, 01; 71-79
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ metodyki badań na ocenę struktury zbiorowisk mikroorganizmów w glebie leśnej
Effect of the methodology of studies on the structure of the microorganisms communities in the forest soil
Autorzy:
Behnke-Borowczyk, J.
Kwaśna, H.
Powiązania:
https://bibliotekanauki.pl/articles/989286.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
gleby lesne
mikroorganizmy glebowe
grzyby mikroskopowe
struktura zbiorowisk
metodyka badan
metody klasyczne
metody molekularne
startery NS1
startery NS2
DNA
region 18S
classical method of isolation
fungi
microorganisms
ns1
ns2
18s rdna
soil
Opis:
Two different communities of microorganisms were identified in soils by application of the classical method of fungi isolation (soil dilution, culturing on artificial media, morphotyping) and a molecular method (extraction of the environmental DNA, amplification with universal primers NS1 and NS2, cloning and sequencing of representative clones). No organisms were common to both communities. Apart from rare representatives of the Animalia, communities included single fungus−like Eucarya belonging to the Protista, Class Oomycota, and numerous fungi belonging to Chytridiomycota, Zygomycota, Ascomycota and Basidiomycota orders. In total, 88 species were identified in four soil samples. Fungi were mostly Ascomycota. The classical method was particularly effective in detection of fungi important for creation of phytosanitary conditions of soil, i.e. antagonists (Penicillium, Tolypocladium and Trichoderma) and potential stimulants (dark−pigmented Hormiactis candida, Humicola spp. and Phialophora spp.) of phytopathogens (including the common forest genera Armillaria and Heterobasidion). Application of the classical method allowed the detection of mycorrhizal Ascomycota from the genus Oidiodendron. Application of the molecular method allowed the detection of 13 mycorrhizal Basidiomycota. Although primers NS1 and NS2 were designed from a match with DNA of culturable organisms, they also amplified the DNA of non−culturable organisms. This emphasizes their potential usefulness in studies of the biodiversity of microorganisms in environmental samples. The shortage of reference sequences in the database discourages use of the 18S rDNA region in studies on fungal communities. The studies on the biodiversity of microorganisms need the application of a few independent methods of detection and identification.
Źródło:
Sylwan; 2016, 160, 06; 492-503
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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