Temat: Hybridization - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: 1;25 centric fusion recognition in the cattle chromosome by fluorescence in situ hybridization
Autorzy:: Ladon, D
Solinas-Toldo, S
Fries, R
Stranzinger, G F
Powiązania:: https://bibliotekanauki.pl/articles/2047680.pdf
Data publikacji:: 1996
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: cattle chromosome
hybridization
fluorescence
cattle
centric fusion
Opis:: Fluorescence in situ hybridization (FISH) experiments with specific probes for chromosome 29 and 25 were carried out on a Brown Swiss bull, previously diagnosed as a carrier of 1;29 centric fusion. The hybridization of the chromosome 29-specific probe (BMC 4216 - already located on 29q13), produced signals on two small acrocentrics, but not on the translocated chromosome. The signals appeared on the translocated chromosome and on a single chromosome 25 after hybridization of the chromosome-specific probe (BMC 3224 - previously located on 25q24). According to the actual nomenclature, the analysed aberration is a robertsonian translocation involving chromosomes 1 and 25.
Źródło:: Journal of Applied Genetics; 1996, 37, 1; 101-104
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Molecular versus classical cytogenetics - evaluation of 20 Prader-Willi syndrome patients
Autorzy:: Stankiewicz, P
Lebiocka, J
Szpecht-Potocka, A
Bocian, E
Stanczak, H
Obersztyn, E
Mazurczak, T
Powiązania:: https://bibliotekanauki.pl/articles/2047150.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: chromosomal deletion
in situ
patient
hybridization
fluorescence
cytogenetics
chromosome region
Prader-Willi syndrome
Opis:: Prader-Willi syndrome (PWS) is a developmental disorder caused by a deficiency of paternal contribution of the chromosome region 15q11.2-q13 arising from differently sized deletions, maternal disomy, or rarely imprinting mutations. We have analyzed 20 PWS patients using combined cytogenetic high resolution technique (HRT), fluorescence in situ hybridization (FISH) and molecular studies to identify parental origin (uniparental disomy) or molecular defect (deletion) of the Prader-Willi region. Lack of a paternal copy of 15q11.2-q13 resulting from its deletion was found in 16 patients. Using high resolution GTG banding on prometaphase chromosomes, deletion in the 15q11.2-q13 region was detected in only 8 patients. Application of FISH with different sets of PWS specific unique sequence probes (D15S11, SNRPN, D15S10, GABRß3) revealed microdeletions in 12 patients. In 12 out of 20 cases FISH confirmed HRT studies, while in 8 cases inconsistent results were obtained. No discrepancies between results of FISH and molecular studies were found, although the latter had a higher sensitivity. We conclude that FISH appears to be a rapid and reliable method of microdeletion identification and should be performed as a method of choice in cytogenetic diagnosis of Prader-Willi syndrome.
Źródło:: Journal of Applied Genetics; 1997, 38, 2; 217-226
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Cytogenetic markers of Brassica napus L. chromosomes
Autorzy:: Hasterok, R
Maluszynska, J.
Powiązania:: https://bibliotekanauki.pl/articles/2043142.pdf
Data publikacji:: 2000
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: oil seed
in situ
silver staining
chromosome number
morphology
molecular cytogenetics
in vitro
karyotype
rDNA
morphometric analysis
plant breeding
oilseed rape
Brassica napus
somatic hybridization
transformation
cytogenetic marker
fluorescence
hybridization
genomic origin
Źródło:: Journal of Applied Genetics; 2000, 41, 1; 1-9
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Evaluation of crossability of Cuphea lanceolata and C. viscosissima based on pollen tube growth and seed set
Autorzy:: Wojciechowski, A
Olejniczak, J
Adamska, E
Powiązania:: https://bibliotekanauki.pl/articles/2047262.pdf
Data publikacji:: 1996
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: pollen tube
fluorescence
Cuphea viscosissima
crossing
seed oil
incompatibility
interspecific hybridization
fatty acid
growth
crossability
Cuphea lanceolata
Opis:: Many species of Cuphea genus show promise as rich natural sources of valuable seed oils for commercial utility. Among them are C. lanceolata and C. viscosissima which seed oils contain medium-chain fatty acids. Reciprocal crosses were made between these species to assess their usefulness for Cuphea breeding program. The degree of crossability between C. lanceolata and C. viscosissima was determined by pollen grain germination and pollen tube growth observed using fluorescence microscopy in addition to capsule formation and seed number per capsule. Treatment of the pollen grain with a laser microbeam reduced the number of germinated pollen grains, tubes growth in the pistil and seed set when C. viscosissima plants were used in the crosses as a female form. In the cross C. lanceolata (female form) × C. viscosissima the pollen treatment with a laser microbeam improved pollen tube growth but reduced seed set. Crossability evaluated on the basis of pollen tube growth and seed set showed unilateral interspecific incompatibility in crosses involving C. lanceolata plants as a female form.
Źródło:: Journal of Applied Genetics; 1996, 37, 3; 277-284
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Cryptic rearrangements of chromosome 12 in testicular germ cell tumors with or without the specific i[12p] marker
Autorzy:: Grygalewicz, B
Pienkowska-Grela, B.
Woroniecka, R.
Powiązania:: https://bibliotekanauki.pl/articles/2043149.pdf
Data publikacji:: 2000
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: aberration
in situ
isochromosome
cytogenetic analysis
microdissection
testicular germ cell tumour
karyotype
cell culture
nonseminoma
fluorescence
hybridization
seminoma
chromosome 12
DNA
Źródło:: Journal of Applied Genetics; 2000, 41, 2; 123-131
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Mechanism, detection and significance of some chromosomal rearrangements in chronic myeloid leukaemia [CML] and acute lymphoblastic leukaemia [ALL]
Autorzy:: Ladon, D
Witt, M.
Powiązania:: https://bibliotekanauki.pl/articles/2043432.pdf
Data publikacji:: 2000
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: translocation
minimal residual disease
in situ
hybridization
fluorescence
chronic myeloid leukemia
chromosome aberration
mixed chimerism
detection
cytogenetic evolution
acute lymphoblastic leukemia
Źródło:: Journal of Applied Genetics; 2000, 41, 3; 187-197
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Molecular follow up of donor lymphocyte infusion in CML children after allogeneic bone marrow transplantation
Autorzy:: Ladon, D
Pieczonka, A.
Jolkowska, J.
Wachowiak, J.
Witt, M.
Powiązania:: https://bibliotekanauki.pl/articles/2048295.pdf
Data publikacji:: 2001
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: minimal residual disease
in situ
human genetics
bone marrow
hybridization
fluorescence
donor lymphocyte infusion
chronic myeloid leukemia
child
polymerase chain reaction
transplantation
Źródło:: Journal of Applied Genetics; 2001, 42, 4; 547-552
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Applications of recent advances at the Institute of Grassland and Environmental Research in cytogenetics of the Lolium-Festuca complex
Autorzy:: Humphreys, M W
Thomas, H M
King, I P
Morgan, W G
Meredith, M R
Harper, J A
Humphreys, M O
Bettany, A J E
Dalton, S J
James, A R
Ougham, H J
Thomas, H
Powiązania:: https://bibliotekanauki.pl/articles/2046675.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: RNA
in situ
tissue culture
gene transfer
chromosome segment
anther culture
androgenesis
plant breeding
introgression mapping
fluorescence
hybridization
Lolium-Festuca complex
drought resistance
gene isolation
tetraploid hybrid
DNA
meiosis
grass
chromosome behaviour
Opis:: Recent advances at Institute of Grassland and Environmental Research (Aberystwyth, U.K.) in cytogenetics of the Lolium/Festuca complex places us in the advantageous position of being able to map genes of agronomic importance onto chromosome arms using fluorescence in situ hybridization (FISH). The ability to physically map genes leads to the capability for "dissecting" quantitative traits into their different components and will lead to better understanding of the complex physiological processes involved and the identification of their genetic control. By tagging genes of interest, using molecular and morphological markers, it will be possible to select and combine suites of desirable genes in a single genotype and thus produce novel cultivars by conventional breeding procedures. Programmes for introgression depend on the relationships between species and on levels of chromosome pairing. Phylogenetic relationships within the Lolium/Festuca complex are being determined using both genomic in situ hybridization (GISH) and FISH. With recent advances in genetic manipulation within the Lolium/Festuca complex, opportunities now arise for gene transfer from Lolium and Festuca species into other important agricultural crops.
Źródło:: Journal of Applied Genetics; 1997, 38, 3; 273-284
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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