Autor: Banbura, M W - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: The xCELLigence system for real-time and label-free analysis of neuronal and dermal cell response to Equine Herpesvirus type 1 infection
Autorzy:: Golke, A.
Cymerys, J.
Slonska, A.
Dzieciatkowski, T.
Chmielewska, A.
Tucholska, A.
Banbura, M.W.
Powiązania:: https://bibliotekanauki.pl/articles/30665.pdf
Data publikacji:: 2012
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: xCELLigence system
real-time analysis
label-free analysis
neuronal cell response
dermal cell response
cell response
equine herpesvirus 1
animal infection
herpesvirus
neuron
neuronal cell
dermal cell
Opis:: Real-time cell electronic sensing (RT-CES) based on impedance measurements is an emerging technology for analyzing the status of cells in vitro. It allows label-free, real time monitoring of the biological status of cells. The present study was designed to assess dynamic data on the cell processes during equine herpesvirus type 1 (EHV-1) infection of ED (equine dermal) cells and primary murine neuronal cell culture. We have demonstrated that the xCELLigence system with dynamic monitoring can be used as a rapid diagnostic tool both to analyze cellular behavior and to investigate the effect of viral infection.
Źródło:: Polish Journal of Veterinary Sciences; 2012, 15, 1
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Equine herpesvirus type 1 (EHV-1) replication in primary murine neurons culture
Autorzy:: Cymerys, J.
Dzieciatkowski, T.
Slonska, A.
Bierla, J.
Tucholska, A.
Chmielewska, A.
Golke, A.
Banbura, M.W.
Powiązania:: https://bibliotekanauki.pl/articles/31974.pdf
Data publikacji:: 2010
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: replication
animal disease
horse
equine herpesvirus 1
respiratory tract infection
neurological disorder
abortion
neuron
Opis:: Equine herpesvirus-1 (EHV-1) infections cause significant economic losses for equine industries worldwide as a result of abortion, respiratory illness, and neurologic disease in all breeds of horses. The occurrence of abortions caused by EHV-1 has repeatedly been confirmed in Poland, but neurological manifestations of the infection have not been described yet. Also it is unknown how the infection of neurons with non-neuropathogenic strains is regulated. To further understand the virus- neuron interaction we studied two strains of EHV-1 in murine primary neuron cell cultures. Both strains were isolated from aborted fetuses: Rac-H, a reference strain isolated by Woyciechowska in 1959 (Woyciechowska 1960) and Jan-E isolated by Bańbura et al. (Bańbura et al. 2000). Upon infection of primary murine neuronal cell cultures with Jan-E or Rac-H strains, a cytopathic effect was observed, manifested by a changed morphology and disintegration of the cell monolayer. Positive results of immunofluorescence, nPCR and real-time PCR tests indicated high virus concentration in neurons, meaning that both EHV-1 strains were likely to replicate in mouse neurons in vitro without the need for adaptation. Moreover, we demonstrated that some neurons may survive (limited) virus replication during primary infection, and these neurons (eight weeks p.i.) harbour EHV-1 and were still able to transmit infection to other cells.
Źródło:: Polish Journal of Veterinary Sciences; 2010, 13, 4
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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