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Wyświetlanie 1-3 z 3
Tytuł:
Usefulness of PCR/RFLP and ERIC PCR techniques for epidemiological study of Haemophilus parasuis infections in pigs
Autorzy:
Jablonski, A.
Zebek, S.
Kolacz, R.
Pejsak, Z.
Powiązania:
https://bibliotekanauki.pl/articles/30649.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
animal disease
pig
animal infection
epidemiology
Haemophilus parasuis
genotyping
polymerase chain reaction
virulence
diagnosis
microorganism
DNA fragment
electrophoretic separation
environmental factor
molecular method
Opis:
Haemophilus parasuis belongs to opportunistic microorganisms of undefined virulence. The purpose of the studies was to compare suitability of PCR/RFLP in our modification and ERIC PCR for epidemiological study of domestic strains of H. parasuis. The results were evaluated taking into account two different aspects: suitability of the tests for isolating the highest possible number of clone groups and subjective evaluation of the method judged with respect to the following criteria: difficulty, availability of equipment and reagents as well as time and cost of the study. The results obtained in the present study show that the two methods used for typing of H. parasuis had high discriminatory power. Taking into account this parameter it can be concluded that ERIC PCR is more suitable than PCR/RFLP. This justifies the use of ERIC PCR for routine epidemiological analyses of mentioned pathogen. Taking into account the complexity of method used, ERIC-PCR based on random amplification of DNA, proved to be comparable to PCR/RFLP. The last mentioned technique is relatively less expensive and labour-consuming, especially when diagnostic PCR method is used for the epidemiological studies.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Retrospective epidemiological study of supracondylar fractures of the humeral bone in children from urban and rural areas of the Lublin region in eastern Poland
Autorzy:
Matuszewski, L.
Okonski, M.
Powiązania:
https://bibliotekanauki.pl/articles/51902.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Tematy:
epidemiology
supracondylar fracture
humerus bone
bone
orthopedics
child
urban area
rural area
Lublin region
Polska
Opis:
Introduction. Supracondylar fractures of the humeral bone are frequent injuries in children. It has been affirmed that supracondylar fractures have an excellent prognosis when proper treatment is applied. Objective. Present of the statistical relationships between fractures occurring and patient’s development period; the relation between development period and site of the fracture and statistical relationship between development period and gender of the patients. Also indicated are the place of residence of the hospitalized patients and time of admission to the Clinic after injury. Materials and method. Research was based on the data of paediatric patients treated in the Clinic for Paediatric Surgery, Traumatology and Paaediatric Orthopaedics, and Rehabilitation Clinic of the Medical University in Lublin, Poland, between 1986- 2010. An independent Chi-square Test was used for statistical analysis (c²). Results. The majority of patients were admitted to the Clinic on the day of injury. Of these patients, 71% lived in the urban area of the Lublin region where all the children received medical care in hospital directly after trauma; 29% of children came from the rural areas of the Lublin region, and 10% of them were admitted to hospital 24 or more hours after the injury. Conclusion. 71% of patients lived in the urban areas of the Lublin region and the main cause of injury was a fall from a higher level onto an outstretched upper left limb. Most supracondylar fractures of the humeral bone concerned children at school and adolescent age. Despite the fact that some of the hospitalised children lived in the rural areas of the Lublin region, the majority were admitted to the Clinic directly after trauma and received timely treatment.
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Clinical and epidemiological characteristics of COVID-19 during the early phase of the SARS-CoV-2 pandemic: a cross-sectional study among medical school physicians and residents employed in a regional reference teaching hospital in Northern Italy
Autorzy:
Dini, Guglielmo
Montecucco, Alfredo
Rahmani, Alborz
Barletta, Chiara
Pellegrini, Luca
Debarbieri, Nicoletta
Orsi, Andrea
Caligiuri, Patrizia
Varesano, Serena
Manca, Alessia
Vargiu, Maria Paola
Di Carlo, Pia
Massa, Emanuela
Icardi, Giancarlo
Durando, Paolo
Powiązania:
https://bibliotekanauki.pl/articles/2086076.pdf
Data publikacji:
2021-05-27
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
occupational health
epidemiology
healthcare workers
clinical characteristics
COVID‑19
SARS-CoV-2
Opis:
ObjectivesThe aim of the study was to evaluate the clinical presentation and burden of SARS-CoV-2 infections among medical school physicians and residents, mainly young medical doctors. The awareness of COVID‑19 clinical manifestations can improve the early detection of mild cases, possibly reducing further transmission to colleagues and patients.Material and MethodsThe study was carried out in March–May 2020, involving medical school physicians in a teaching hospital in northern Italy, with a working population of 881 medical doctors. Data collection was performed using a structured form investigating clinical and epidemiological information.ResultsOne hundred sixty-two medical doctors contacted the Occupational Health Service reporting acute respiratory symptoms or close contact exposure to a confirmed COVID‑19 case. Among the confirmed COVID‑19 cases, most were male doctors during residency, and 85% presented a mild clinical picture. Fever (70.3%) and cough (51.4%) represented the most prevalent symptoms of COVID‑19. As revealed by the univariate analysis, the prevalence of real-time reverse transcriptase-polymerase chain reaction (RT-PCR) positivity increased with age (OR = 1.08, 95% CI: 1.02–1.14, p = 0.012), working in a COVID‑19 ward (OR = 3.33, 95% CI: 1.09–10.21, p = 0.031), presenting alteration or loss of smell/taste (OR = 10.00, 95%CI: 2.80–35.69, p < 0.001) and myalgia (OR = 3.20, 95% CI: 1.00–10.26, p = 0.046), while being a resident (OR = 0.20, 95% CI: 0.05–0.80, p = 0.030) was associated with reduced odds of being infected, compared to staff physicians. Age and loss of smell/taste were the only factors independently associated with RT-PCR positivity.ConclusionsThe majority of COVID‑19 cases showed a mild clinical syndrome, ranging from absence or paucity of symptoms to common cold or influenza-like symptoms. The findings of the present study increase the accuracy of the clinical diagnosis for the prompt identification and management of suspected COVID‑19 cases, being particularly useful during resurges of the SARS-CoV-2 pandemic.
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2021, 34, 2; 189-201
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-3 z 3

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