- Tytuł:
- Characterization of phytoplasmas related to ‘Candidatus Phytoplasma asteris’ subgroup rpI-L in Iran
- Autorzy:
-
Vali-Sichani, F.
Bahar, M.
Zirak, L. - Powiązania:
- https://bibliotekanauki.pl/articles/66181.pdf
- Data publikacji:
- 2014
- Wydawca:
- Polska Akademia Nauk. Czytelnia Czasopism PAN
- Tematy:
-
phytoplasma
disease symptom
plant
disease
polymerase chain reaction
seed
Candidatus Phytoplasma asteris
rpI-L subgroup
rp gene
tuf gene
Iran - Opis:
- In two of Iran's central provinces, several herbaceous plants showing phytoplasma disease symptoms were collected to detect 'Canididatus Phytoplasma asteris'-related phytoplasmas. Confirmation of an association of phytoplasmas with diseased plants was done using polymerase chain reaction (PCR) assays having the phytoplasma universal primer pairs P1/P7 followed by R16F2n/ R16R2 in nested PCR. Then, for detection of 'Ca. P. asteris', DNA samples were subjected to amplification of rp and tuf genes using specific primer pairs rp(I)F1A/rp(I)R1A and fTufAy/rTufAy, respectively. Restriction fragment length polymorphism or RFLP analyses of rp gene fragments using Tsp509I restriction enzyme as well as sequence analyses indicated that 'Ca. P. asteris'-related phytoplasmas associated with carrot, niger seed and scallion plants in these regions, belong to the rpI-L subgroup. This research is the first report of carrot, niger seed, and scallion infection with phytoplasmas belonging to the rpI-L subgroup.
- Źródło:
-
Journal of Plant Protection Research; 2014, 54, 2
1427-4345 - Pojawia się w:
- Journal of Plant Protection Research
- Dostawca treści:
- Biblioteka Nauki
Artykuł