- Tytuł:
- Column chromatography as a useful step in purification of diatom pigments
- Autorzy:
-
Tokarek, Wiktor
Listwan, Stanisław
Pagacz, Joanna
Leśniak, Piotr
Latowski, Dariusz - Powiązania:
- https://bibliotekanauki.pl/articles/1038760.pdf
- Data publikacji:
- 2016
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
column chromatography
Phaeodactylum tricornutum
fucoxanthin
diadinoxanthin
diatoxanthin
thin layer chromatography - Opis:
- Fucoxanthin, diadinoxanthin and diatoxanthin are carotenoids found in brown algae and most other heterokonts. These pigments are involved in photosynthetic and photoprotective reactions, and they have many potential health benefits. They can be extracted from diatom Phaeodactylum tricornutum by sonication, extraction with chloroform : methanol and preparative thin layer chromatography. We assessed the utility of an additional column chromatography step in purification of these pigments. This novel addition to the isolation protocol increased the purity of fucoxanthin and allowed for concentration of diadinoxanthin and diatoxanthin before HPLC separation. The enhanced protocol is useful for obtaining high purity pigments for biochemical studies.
- Źródło:
-
Acta Biochimica Polonica; 2016, 63, 3; 443-447
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł