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Wyszukujesz frazę "cryopreservation" wg kryterium: Temat

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    Wyświetlanie 1-4 z 4
    Tytuł:
    Changes in cell physiology at the stage of dehydration in a cryopreservation protocol
    Autorzy:
    Domzalska, L.
    Kedracka-Krok, S.
    Mikula, A.
    Rybczynski, J.J.
    Powiązania:
    https://bibliotekanauki.pl/articles/951293.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    cryopreservation
    cell physiology
    dehydration
    Gentiana cruciata
    proteomic analysis
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Sensitivity of Tilia cordata seeds to dehydration and temperature of liquid nitrogen
    Autorzy:
    Chmielarz, P
    Powiązania:
    https://bibliotekanauki.pl/articles/41625.pdf
    Data publikacji:
    2002
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    dehydration
    drying
    liquid nitrogen
    temperature
    Tilia cordata
    forest habitat
    sensitivity
    scarification
    cryopreservation
    seed
    Opis:
    The aim of the study was to assess the susceptibility of small-leaved lime (Tilia cordata Mill.) seeds to drying and freezing in liquid nitrogen (-196°C). Seed samples were frozen in liquid nitrogen for 24 h at 11 different levels of seed moisture content (m.c.), ranging from 3.1% to 22.8% (fresh weight basis). All samples, including unfrozen control samples, were subjected to scarification with concentrated sulphuric acid (Tylkowski 1998) either before or after freezing. Seed pre-treatment before germination (at 3~15°C/16~8h) involved cold stratification at 3°C without substrate. Seed drying to 3.1% m.c. significantly reduced their germinability (to 63%), as compared to the high germinability (82-88%) of seeds with 5.2-20.9% m.c. Thus seeds of this species can be assigned to the ‘suborthodox' category. Such a high germinability (79-87%) was preserved after freezing in liquid nitrogen in samples dried to 9.0-17.4% m.c. if scarification was performed before freezing, and in samples dried to 9.1-16.2% m.c. if scarification was performed after freezing. The highest percentage of seedlings emerged after freezing in liquid nitrogen from seeds dried to 11.1-20.1% m.c. (emergence 65-75%) if scarification was performed before freezing, and from seeds dried to 7.3-17.8% m.c. (emergence 53%-71%) if scarification was performed after freezing.
    Źródło:
    Dendrobiology; 2002, 47 Supplement
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of abscisic acid and dimethyl sulfoxide and different temperatures on the cryopreservation process of Abies nordmanniana (Steven) Spach embryogenic callus
    Autorzy:
    Nawrot-Chorabik, K.
    Sitko, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/79904.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    cryopreservation
    liquid nitrogen
    somatic embryo
    embryogenic callus
    Abies nordmanniana
    abscisic acid
    dimethyl sulphoxide
    dehydration
    callus cell
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of various preculture and osmotic dehydration conditions on cryopreservation efficiency and morphogenetic response of chrysanthemum shoot tips
    Autorzy:
    Kulus, D.
    Serocka, M.
    Mikuła, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/11880881.pdf
    Data publikacji:
    2018
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    mother chrysanthemum
    Dendranthema grandiflora
    Lady Orange cultivar
    Lady Salmon cultivar
    Richmond cultivar
    plant cultivar
    ornamental plant
    flowering plant
    chimera
    shoot tip
    explant
    cryopreservation
    dehydration
    osmotic dehydration
    morphogenetic response
    sucrose concentration
    preculture treatment
    Opis:
    The aim of this study was to evaluate the influence of sucrose concentration in the preculture medium, as well as the duration of osmotic dehydration on the efficiency of chrysanthemum ‘Richmond’, ‘Lady Orange’ and ‘Lady Salmon’ cryopreservation by encapsulation-dehydration technique. For all cultivars tested, the best regrowth of cryopreserved shot tips was recorded with 0.25 M sucrose concentration and 10 µM ABA during a two-week preculture, followed by a 4- or 7-day osmotic dehydration. The survival rate ranged from 56.8–58.0% (the Lady group) to 63.6% (‘Richmond’). However, the ability to grow was smaller and reached 18.2–50.7%. It was found that higher sucrose concentration during the preculture slowed the growth of chrysanthemum shoot tips and led to an increased formation of multiple shoots (by activating axillary buds) or deformed adventitious shoots (incapable of further growth). The frequency of tissue hyperhydricity also increased, while the rhizogenesis efficiency decreased when higher sucrose concentration in the preculture medium was applied. The influence of osmotic dehydration duration on the explants morphogenetic response was cultivar-dependent.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2018, 17, 1; 139-147
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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