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    Wyświetlanie 1-11 z 11
    Tytuł:
    Wykrywanie Borrelia burgdorferi sensu lato w kleszczach Ixodes ricinus metoda lancuchowej reakcji polimerazy [PCR]
    Autorzy:
    Skotarczak, B
    Powiązania:
    https://bibliotekanauki.pl/articles/838375.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    czynniki chorobotworcze
    kleszcze
    wykrywanie drobnoustrojow
    przenoszenie chorob
    parazytologia
    Ixodes ricinus
    lancuchowa reakcja polimerazy
    Borrelia burgdorferi
    Opis:
    Attempts were made to identify the causative orgamsm of Lyme disease in Szczecin from tick Ixodes ricinus as a vector. Ticks were collected in 1997 year in forest areas of Szczecin, from localites associated with numerous attendance of people. The method used in this study was the polymerase chain reaction (PCR) on the flagellin structural gene fla of Borrelia burgdorferi sensu stricto. The flagellin PCR primer set reaction was conservative for B. burgdorferi sensu stricto, B. afzelii and B. garinii. The overall prevalence of B. burgdorferi sensu lato, in tick population studied was 8.8%. The female, nymphs and larves of Ixodes ricinus were infected almost just the some - about 10%, when the male 2.5% only.
    Źródło:
    Annals of Parasitology; 2000, 46, 1; 93-99
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wykrywanie Borrelia burgdorferi sensu lato w kleszczach Ixodes ricinus metoda łańcuchowej reakcji polimerazy [PCR]
    Autorzy:
    Skotarczak, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/2148520.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    czynniki chorobotworcze
    kleszcze
    wykrywanie drobnoustrojow
    przenoszenie chorob
    parazytologia
    Ixodes ricinus
    lancuchowa reakcja polimerazy
    Borrelia burgdorferi
    Opis:
    Attempts were made to identify the causative orgamsm of Lyme disease in Szczecin from tick Ixodes ricinus as a vector. Ticks were collected in 1997 year in forest areas of Szczecin, from localites associated with numerous attendance of people. The method used in this study was the polymerase chain reaction (PCR) on the flagellin structural gene fla of Borrelia burgdorferi sensu stricto. The flagellin PCR primer set reaction was conservative for B. burgdorferi sensu stricto, B. afzelii and B. garinii. The overall prevalence of B. burgdorferi sensu lato, in tick population studied was 8.8%. The female, nymphs and larves of Ixodes ricinus were infected almost just the some - about 10%, when the male 2.5% only.
    Źródło:
    Wiadomości Parazytologiczne; 2000, 46, 1; 93-99
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wystepowanie kretkow Borrelia burgdorferi s.l. u kleszczy Ixodes ricinus w lasach wojewodztwa szczecinskiego
    Autorzy:
    Skotarczak, B
    Wodecka, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/841322.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    czynniki chorobotworcze
    kleszcze
    woj.szczecinskie
    przenoszenie chorob
    parazytologia
    lasy
    Ixodes ricinus
    Borrelia burgdorferi
    bakterie
    kretki
    Opis:
    Presently acquired results are the outcome of, as for today, pilot-scale, preliminary study, although they were based on a tick (Ixodes ricinus) population of 533 individuals, collected from the vicinity of Szczecin. The areas sampled have been considered as recreational, which means they are frequented by a large number of people. The ticks were collected in 1996 in two seasons: spring-summer and summer-autumn. The overall prevalence of the spirochetes B. burgdorferi sensu lato, in the tick population studied, was 12.6%, while that of the spring-summer season reached 25.5%, decreasing in the summer-autumn season down to 2.3%.
    Źródło:
    Annals of Parasitology; 1998, 44, 2; 227-232
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wystepowanie Babesia microti w kleszczach Ixodes ricinus na wybranych terenach Pomorza Zachodniego
    Autorzy:
    Skotarczak, B.
    Sawczuk, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/837033.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    wektory chorob
    wystepowanie
    czynniki chorobotworcze
    kleszcze
    pierwotniaki
    parazytologia
    Ixodes ricinus
    Babesia microti
    Pomorze Zachodnie
    Źródło:
    Annals of Parasitology; 2003, 49, 3; 273-280
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wstępne badania czynnika etiologicznego ludzkiej ehrlichiozy [HGE] w kleszczach z zachodniopółnocnej Polski
    Autorzy:
    Skotarczak, B.
    Rymaszewska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2147844.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    wektory chorob
    czynniki chorobotworcze
    Polska Zachodnio-Polnocna
    choroby czlowieka
    kleszcze
    parazytologia
    etiologia
    erlichioza
    Ehrlichia
    Opis:
    Ehrlichias occur in ticks in the cells of their haemolymph-hematocytes. They enter the vertebrate host organism with the saliva of the tick, during a blood meal. Humans can also be the hosts for this pathogen. Two pathogens cause a humane disease-monocytic ehrlichiasis (E. chaffensis) or granulocytic ehrlichiasis (HGE factor). The above disease units are difficult to diagnose because of their non-specific symptoms. A preliminary study has been conducted on the prevalence of the HGE factor in the ticks, Ixodes ricinus in the recreational areas of the West-Pomeranian Province. All forms of I. ricinus were collected from 3 sites. All the sites are known to be frequented by hikers and gatherers of forest mushrooms and berries. The site selection involved also careful consideration of the tree- and underbrush type. The ticks were collected twice a year in spring (May/June) and in autumn (August\September), which was associated with the biological activity of the collected acarines. A total of 1159 Ixodes ricinus ticks were collected, in this number 172 females, 167 males, 597 nymphs, and 223 larvae. Using the PCR technique, the 16SrRNA-gene fragment was amplified using primers specific for the HGE factor: EHR 790 and EHR 521. The studied population contained 3.7% infected females in spring and 2.7% in autumn, 0.68% infected males in spring, no infected in autumn. The nymphs were infected in spring (2.17%) and in autumn too (0.73%), but the larvae were not infected in both seasons. Analysing the above-mentioned results it can be concluded that the decisive majority of the individuals transmitting the HGE factor are the adult forms.The present study was only a preliminary one. In the future much more sites will be monitored, in the recreational areas of both the city of Szczecin and the entire province.
    Źródło:
    Wiadomości Parazytologiczne; 2001, 47, 1; 95-101
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Capreolus capreolus i Ixodes ricinus jako rezerwuar Bartonella w Polsce Północno-Zachodniej
    Autorzy:
    Skotarczak, B.
    Adamska, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2146380.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    czynniki chorobotworcze
    kleszcze
    rezerwuary zarazka
    parazytologia
    Ixodes ricinus
    Capreolus capreolus
    Bartonella
    Polska Polnocno-Zachodnia
    sarna
    Opis:
    Capreolus capreolus and Ixodes ricinus as a reservoir of Bartonella in north-western Poland. The purpose of the study was to assess the prevalence Bartonella in Capreolus capreolus from north-western Poland forest. Supplementary, ticks infesting roe deer were also screened in order to ascertain their role as vectors and reservoirs of Bartonella. The samples of blood from 98 animals from north-western Poland were PCR-screened. Bartonella DNA was detected by using primers complementary to the intergenic spacer (ITS) between the 16S and 23S rRNA genes, which is used for identification of over a dozen species of this genus. Products of three different sizes were detected: 230 bp and 290 bp may represent two strains of B. capreoli, and 190 bp may be identify as B. bovis. All three amplicons were detected in blood, the 290 bp fragment from B. capreoli was present only in ticks, Ixodes ricinus. Generally, Bartonella infection in C. capreolus amounted to 21.4% of individuals, but was much higher during the autumn-winter seasons (62%), than in spring (4.3%). The results show that C. capreolus may be a reservoir for at least two species, i.e. B. capreoli and B. bovis, and probably do not cause persistent infection in roe deer. The high percentage of infested individuals during spring (84%) and infection detected in I. ricinus (5.2%) show that ticks are reservoir and vector of Bartonella.
    Źródło:
    Wiadomości Parazytologiczne; 2005, 51, 2; 139-143
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Występowanie krętków Borrelia burgdorferi s.l. u kleszczy Ixodes ricinus w lasach województwa szczecińskiego
    OCCURRENCE OF SPIROCHETES BORRELIA BURGDORFERI S. L. IN TICKS IXODES RICINUS IN THE FORESTS OF SZCZECIN PROVINCE
    Autorzy:
    Skotarczak, B.
    Wodecka, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/2148872.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    czynniki chorobotworcze
    kleszcze
    woj.szczecinskie
    przenoszenie chorob
    parazytologia
    lasy
    Ixodes ricinus
    Borrelia burgdorferi
    bakterie
    kretki
    Opis:
    Presently acquired results are the outcome of, as for today, pilot-scale, preliminary study, although they were based on a tick (Ixodes ricinus) population of 533 individuals, collected from the vicinity of Szczecin. The areas sampled have been considered as recreational, which means they are frequented by a large number of people. The ticks were collected in 1996 in two seasons: spring-summer and summer-autumn. The overall prevalence of the spirochetes B. burgdorferi sensu lato, in the tick population studied, was 12.6%, while that of the spring-summer season reached 25.5%, decreasing in the summer-autumn season down to 2.3%.
    Źródło:
    Wiadomości Parazytologiczne; 1998, 44, 2; 227-232
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wstepne badania czynnika etiologicznego ludzkiej ehrlichiozy [HGE] w kleszczach z zachodniopolnocnej Polski
    Autorzy:
    Skotarczak, B
    Rymaszewska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/839153.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    wektory chorob
    czynniki chorobotworcze
    Polska Zachodnio-Polnocna
    choroby czlowieka
    kleszcze
    parazytologia
    etiologia
    erlichioza
    Ehrlichia
    Opis:
    Ehrlichias occur in ticks in the cells of their haemolymph-hematocytes. They enter the vertebrate host organism with the saliva of the tick, during a blood meal. Humans can also be the hosts for this pathogen. Two pathogens cause a humane disease-monocytic ehrlichiasis (E. chaffensis) or granulocytic ehrlichiasis (HGE factor). The above disease units are difficult to diagnose because of their non-specific symptoms. A preliminary study has been conducted on the prevalence of the HGE factor in the ticks, Ixodes ricinus in the recreational areas of the West-Pomeranian Province. All forms of I. ricinus were collected from 3 sites. All the sites are known to be frequented by hikers and gatherers of forest mushrooms and berries. The site selection involved also careful consideration of the tree- and underbrush type. The ticks were collected twice a year in spring (May/June) and in autumn (August\September), which was associated with the biological activity of the collected acarines. A total of 1159 Ixodes ricinus ticks were collected, in this number 172 females, 167 males, 597 nymphs, and 223 larvae. Using the PCR technique, the 16SrRNA-gene fragment was amplified using primers specific for the HGE factor: EHR 790 and EHR 521. The studied population contained 3.7% infected females in spring and 2.7% in autumn, 0.68% infected males in spring, no infected in autumn. The nymphs were infected in spring (2.17%) and in autumn too (0.73%), but the larvae were not infected in both seasons. Analysing the above-mentioned results it can be concluded that the decisive majority of the individuals transmitting the HGE factor are the adult forms.The present study was only a preliminary one. In the future much more sites will be monitored, in the recreational areas of both the city of Szczecin and the entire province.
    Źródło:
    Annals of Parasitology; 2001, 47, 1; 95-101
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Występowanie Babesia microti w kleszczach Ixodes ricinus na wybranych terenach Pomorza Zachodniego
    Occurrence of Babesia microti in ticks Ixodes ricinus on selected areas of Western Pomerania
    Autorzy:
    Skotarczak, B.
    Sawczuk, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2147657.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    wektory chorob
    wystepowanie
    czynniki chorobotworcze
    kleszcze
    pierwotniaki
    parazytologia
    Ixodes ricinus
    Babesia microti
    Pomorze Zachodnie
    Opis:
    The aim of present study was to evaluate acquisition risk of babesiosis in human population exposed to ticks /xodes ricinus by examination of Babesia microti DNA occurrence in ticks of all development stages. The polymerase chain reaction (PCR) was applied to estimate the occurrence of DNA Babesia microti in Ixodes ricinus. The Bab1 and Bab4 primers were used to amplify fragment, 238bp in length, of 18S rRNA gene for small ribosomal subunit. Amplicons were electroforeticaly separated in agarose gels. Ticks were collected in year 1999 and 2000, twice in each year in spring-summer (May-July) and summer-autumn (August-October) seasons from Goleniów Forest and Pobierowo. These places have been classified as people attendance and tourist areas. The 716 /. ricinus ticks were collected in 1999 with 61.3% of nymphs, 17.8% larvae, 10.9% females and 9.9% males. Highest range of infection was observed in females — 28.8% studied, than males — 18.3%, nymphs — 7.7% and larvae — 3.1%. The total number of 416 /. ricinus was collected in year 2000 with 64% of nymphs, 13.4% males, 11.9% females and 10.7% larvae. The infection with Babesia microti occurred only in three nymphs, which was 0.7% of studied population.
    Źródło:
    Wiadomości Parazytologiczne; 2003, 49, 3; 273-280
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Anaplasma [Ehrlichia] phagocytophila i pierwotniaki z rodzaju Babesia u psow na terenach endemicznych dla choroby z Lyme w polnocno-zachodniej Polsce
    Autorzy:
    Skotarczak, B.
    Adamska, M.
    Rymaszewska, A.
    Supron, M.
    Sawczuk, M.
    Maciejewska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/841336.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    wektory chorob
    psy
    czynniki chorobotworcze
    kleszcze
    pierwotniaki
    Anaplasma phagocytophilum
    parazytologia
    wspolwystepowanie
    borelioza
    Polska Polnocno-Zachodnia
    bakterie
    Babesia
    Źródło:
    Annals of Parasitology; 2004, 50, 3; 555-561
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Anaplasma [Ehrlichia] phagocytophila i pierwotniaki z rodzaju Babesia u psów na terenach endemicznych dla choroby z Lyme w północno-zachodniej Polsce
    Anaplasma phagocytophila and protozoans of Babesia genus in dogs from endemic areas of Lyme disease in north-western Poland
    Autorzy:
    Skotarczak, B.
    Adamska, M.
    Rymaszewska, A.
    Suproń, M.
    Sawczuk, M.
    Maciejewska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2147449.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    wektory chorob
    psy
    czynniki chorobotworcze
    kleszcze
    pierwotniaki
    Anaplasma phagocytophilum
    parazytologia
    wspolwystepowanie
    borelioza
    Polska Polnocno-Zachodnia
    bakterie
    Babesia
    Opis:
    Infections caused by the spirochete Borrelia burgdorferi sensu lato may be accompanied by other microorganisms, such as Anaplasma, Ehrlichia and Babesia. These pathogens are transmitted by the ticks and are a risk to humans and animals. Ixodes ricinus ticks collected from recreational areas of Szczecin and northwestern Poland contained DNA of the pathogens mentioned above and cases of double and triple coinfection have been documented. The aim of this paper was to determine if dogs suspect to tick infestation in the area of study are a reservoir for these pathogens and to examine the possibility of coinfection. Canine blood was sampled, part of the material originated from dogs exhibiting symptoms of borreliosis. In an earlier study, the samples were screened for DNA from B. burgdorferi sensu lato. In order to screen for A. phagocytophila and Babesia sp. DNA, a PCR-based method was used with the following primers: EHR521/EHR747 for Anaplasma and FOR1/REV1 for Babesia. In 192 samples only two contained A. phagocytophila DNA. One of these samples originated from a healthy canine, the other from an individual with symptoms of borreliosis. The examined samples were not positive for Babesia sp. DNA. Coinfection was not discovered. The low level of A. phagocytophila infection may indicate that the domestic dog is not a reservoir for Anaplasma and Babesia in Szczecin and northwestern Poland. Moreover, this area does not have populations of the brown dog tick (Rhipicephalus sanguineus) or Dermacentor reticulates — both of which are vectors of E. canis and B. canis and commonly induce ehrlichiosis and babesiosis in canines.
    Źródło:
    Wiadomości Parazytologiczne; 2004, 50, 3; 555-561
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-11 z 11

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