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    Wyświetlanie 1-5 z 5
    Tytuł:
    Assessment of in situ-Prepared Polyvinylpyrrolidone-Silver Nanocomposite for Antimicrobial Applications
    Autorzy:
    El Hotaby, W.
    Sherif, H.
    Hemdan, B.
    Khalil, W.
    Khalil, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/1032332.pdf
    Data publikacji:
    2017-06
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    silver nanoparticles
    polyvinylpyrrolidone
    nanocomposite
    antimicrobial activity
    cytotoxicity
    Opis:
    Polyvinylpyrrolidone (PVP) is employed in several potential applications, relying of its special chemical and physical properties in addition to its low toxicity and biocompatibility. The aim of this work is to prepare polyvinylpyrrolidone-silver (PVP-Ag) nanocomposite with high inhibiting effect on the microbial growth and low cytotoxicity. In situ prepared small stable spherical silver nanoparticles, with narrow range particle size distribution, were obtained by easy, economical and rapid chemical reduction method. Silver ions were reduced to silver nanoparticles using low amount of sodium borohydride (NaBH₄) as a strong reducing agent. PVP-Ag nanocomposite was prepared using PVP as a stabilizing and capping agent. Formation of the spherical silver nanoparticles with mean particle size 5 nm was confirmed by ultraviolet-visible spectroscopy, high resolution transmission electron microscopy, and dynamic light scattering. The inhibiting growth effect of the nanocomposite toward Gram-positive bacteria (Staphylococcus aureus), Gram-negative bacteria (Pseudomonas aeruginosa), and yeast fungus (Candida albicans) were studied. The cytotoxicity of the nanocomposite against BJ1 normal skin fibroblast cell line was tested. Results of this work presented perfect antimicrobial activity of the PVP-Ag nanocomposite towards bacteria and fungi with low cytotoxicity, which may lead to promising applications in skin wound healing.
    Źródło:
    Acta Physica Polonica A; 2017, 131, 6; 1554-1560
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Synthesis and antimicrobial evolution of some new benzotriazole substituted 1,3,4-thiadiazoles
    Autorzy:
    Godhani, Dinesh R.
    Mulani, Vishal B.
    Mehta, Jignasu P.
    Kukadiya, Nipul B.
    Powiązania:
    https://bibliotekanauki.pl/articles/1177753.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    antimicrobial activity
    benzotriazole
    cytotoxicity
    haemolysis
    schiff base
    thiadiazole
    Opis:
    A series of novel benzotriazole-substituted 1,3,4-thiadiazole-2(3H)-thione derivatives was synthesized by cyclization of Schiff base. The structures were recognized on the foundation of spectral tools and their purity by elemental analysis. All the compounds were preliminary evaluated for their in vitro antimicrobial activities against five bacterial strains viz [Staphylococcus aureus (MRSA; ATCC 43300), Klebsiella pneumoniae (ATCC 700603), Escherichia coli (ATCC 25922), Acinetobacter baumannii (ATCC 19606), Pseudomonas aeruginosa (ATCC 27853)] and two fungi Strains viz. [Candida albicans (ATCC 90028), Cryptococcus neoformans (ATCC 208821)]. Several compounds exhibited remarkable antimicrobial activity, out of 15 compounds, three compounds viz. 5a, 5g and 5i showed promising antifungal activities with no signs of human cells cytotoxic [HEK293: Human Embryonic Kidney cells (ATCC CRL-1573)] and haemolytic activity [Whole blood (ARCBS 5400 00150)].
    Źródło:
    World Scientific News; 2018, 100; 51-60
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Synergistic hemolysins of coagulase-negative staphylococci (CoNS)
    Autorzy:
    Różalska, Małgorzata
    Derczyńska, Anna
    Maszewska, Agnieszka
    Powiązania:
    https://bibliotekanauki.pl/articles/1038908.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    coagulase-negative staphylococci
    CoNS
    synergistic hemolysin
    antimicrobial activity
    cytotoxicity
    Opis:
    A total of 104 coagulase negative staphylococci, belonging to S. capitis, S. hominis, S. haemolyticus and S. warneri, originating from the collection of the Department of Pharmaceutical Microbiology (ZMF), Medical University of Lodz, Poland, were tested for their synergistic hemolytic activity. 83% of strains produced δ-hemolysin, however, the percentage of positive strains of S. haemolyticus, S. warneri, S. capitis and S. hominis was different - 98%, 78%, 75% and 68%, respectively. Highly pure hemolysins were obtained from culture supernatants by protein precipitation with ammonium sulphate (0-70% of saturation) and extraction by using a mixture of organic solvents. The purity and molecular mass of hemolysins was determined by TRIS/Tricine PAGE. All CoNS hemolysins were small peptides with a molar mass of about 3.5 kDa; they possessed cytotoxic activity against the line of human foreskin fibroblasts ATCC Hs27 and lysed red cells from different mammalian species, however, the highest activity was observed when guinea pig, dog and human red blood cells were used. The cytotoxic effect on fibroblasts occurred within 30 minutes. The S. cohnii ssp. urealyticus strain was used as a control. The antimicrobial activity was examined using hemolysins of S. capitis, S. hominis, S. cohnii ssp. cohnii and S. cohnii ssp. urealyticus. Hemolysins of the two S. cohnii subspecies did not demonstrate antimicrobial activity. Cytolysins of S. capitis and S. hominis had a very narrow spectrum of action; out of 37 examined strains, the growth of only Micrococcus luteus, Corynebacterium diphtheriae and Pasteurella multocida was inhibited.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 4; 757-764
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cyclization and antimicrobial evolution of 1,3,4-oxadiazoles by carbohydrazide
    Autorzy:
    Godhani, Dinesh R.
    Mulani, Vishal B.
    Mehta, Jignasu P.
    Powiązania:
    https://bibliotekanauki.pl/articles/1063054.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    1
    3
    4-oxadiazole
    antimicrobial activity
    cytotoxicity
    dihydropyrimidine
    haemolysis
    Opis:
    A series of dihydropyrimidine substituted 1,3,4-oxadiazole derivatives was synthesized by cyclization of carbohydrazide by phosphoryl chloride and benzoic acid in acidic condition. The structures were perceived on the establishment of spectral tools and their purity by elemental analysis. Every compound was primary assessed for their in vitro antimicrobial activities against 5 bacterial strains viz [Staphylococcus aureus (MRSA; ATCC 43300), Klebsiella pneumoniae (ATCC 700603), Escherichia coli (ATCC 25922), Acinetobacter baumannii (ATCC 19606), Pseudomonas aeruginosa (ATCC 27853)] and 2 fungi Strains viz. [Candida albicans (ATCC 90028), Cryptococcus neoformans var. grubii (H99; ATCC 208821)]. Some compounds displayed significant antimicrobial activity, out of 15 compounds, 11 compounds indicated promising antifungal activities without any indications of human cells cytotoxic [Hk: Human Embryonic Kidney cells (ATCC CRL-1573)] and haemolytic activity [RBC (ARCBS 5400 00150)].
    Źródło:
    World Scientific News; 2019, 124, 2; 304-311
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Assessment of cytotoxic and antimicrobial activity of selected gingival haemostatic agents – in vitro study
    Autorzy:
    Szymonowicz, Maria
    Rusak, Agnieszka
    Pajączkowska, Magdalena
    Nowicka, Joanna
    Wiśniewska, Kamila
    Żywicka, Bogusława
    Rybak, Zbigniew
    Dobrzyński, Maciej
    Powiązania:
    https://bibliotekanauki.pl/articles/27324123.pdf
    Data publikacji:
    2020
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    haemostatic agents
    cytotoxicity
    gingiva retraction
    antimicrobial activity
    środki hemostatyczne
    cytotoksyczność
    retrakcja dziąsła
    Aktywność przeciwbakteryjna
    Opis:
    Purpose: The present research aimed to determine whether and how the aluminium chloride – based materials affect the cell line of the bacterial line and fungi. Methods: Cytotoxicity of haemostatic astringents: Alustat (liquid), Alustat (gel), Alustat (foam), Alustin, Hemostat, Racestyptine and Traxodent containing AlCl3 was conducted on L929 cell line with the use of MTT and SRB assays. The antimicrobial activity (CFU and MIC) against C. albicans, S. mutans, L. rhamnosus was determined. Results: In the MTT results, cell viability for all agents were very low. In SRB, the lowest cytotoxicity was demonstrated for Hemostat and Alustat (foam), Traxodent and Racestyptine. Total reduction of the CFU of S. mutans was observed. Alustat (gel) and Alustat (liquid) completely inhibited the growth of C. albicans, S. mutans and L. rhamnosus. Conclusions: The viability of L929 cells obtained in the SRB assay is more reliable than that obtained in the MTT assay, in the case of gingival haemostatic agents.
    Źródło:
    Acta of Bioengineering and Biomechanics; 2020, 22, 3; 185--198
    1509-409X
    2450-6303
    Pojawia się w:
    Acta of Bioengineering and Biomechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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