Autor: Mikula, A. - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Encapsulation-dehydration technique for chrysanthemum “Lady Orange” cryopreservation
Autorzy:: Kulus, D.
Rewers, M.
Mikula, A.
Powiązania:: https://bibliotekanauki.pl/articles/951265.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: ornamental plant
chrysanthemum
plant material
horticultural production
somaclonal variation
cryopreservation
long-term storage
encapsulation-dehydration method
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Effect of various preculture and osmotic dehydration conditions on cryopreservation efficiency and morphogenetic response of chrysanthemum shoot tips
Autorzy:: Kulus, D.
Serocka, M.
Mikuła, A.
Powiązania:: https://bibliotekanauki.pl/articles/11880881.pdf
Data publikacji:: 2018
Wydawca:: Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:: plant cultivation
mother chrysanthemum
Dendranthema grandiflora
Lady Orange cultivar
Lady Salmon cultivar
Richmond cultivar
plant cultivar
ornamental plant
flowering plant
chimera
shoot tip
explant
cryopreservation
dehydration
osmotic dehydration
morphogenetic response
sucrose concentration
preculture treatment
Opis:: The aim of this study was to evaluate the influence of sucrose concentration in the preculture medium, as well as the duration of osmotic dehydration on the efficiency of chrysanthemum ‘Richmond’, ‘Lady Orange’ and ‘Lady Salmon’ cryopreservation by encapsulation-dehydration technique. For all cultivars tested, the best regrowth of cryopreserved shot tips was recorded with 0.25 M sucrose concentration and 10 µM ABA during a two-week preculture, followed by a 4- or 7-day osmotic dehydration. The survival rate ranged from 56.8–58.0% (the Lady group) to 63.6% (‘Richmond’). However, the ability to grow was smaller and reached 18.2–50.7%. It was found that higher sucrose concentration during the preculture slowed the growth of chrysanthemum shoot tips and led to an increased formation of multiple shoots (by activating axillary buds) or deformed adventitious shoots (incapable of further growth). The frequency of tissue hyperhydricity also increased, while the rhizogenesis efficiency decreased when higher sucrose concentration in the preculture medium was applied. The influence of osmotic dehydration duration on the explants morphogenetic response was cultivar-dependent.
Źródło:: Acta Scientiarum Polonorum. Hortorum Cultus; 2018, 17, 1; 139-147
1644-0692
Pojawia się w:: Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: A simple way to overcome the recalcitrance of the water fern Ceratopteris thalictroides (L.) Brongn. to cryopreservation
Autorzy:: Makowski, D.
Rybczynski, J.J.
Mikula, A.
Powiązania:: https://bibliotekanauki.pl/articles/57732.pdf
Data publikacji:: 2015
Wydawca:: Polskie Towarzystwo Botaniczne
Tematy:: abscisic acid
water fern
fern
Ceratopteris thalictroides
cryopreservation
encapsulation-dehydration method
darkness
Opis:: Ceratopteris thalictroides is a water fern very sensitive to both dehydration and low temperature. This study focuses on the cryopreservation of this species by encapsulation-dehydration technique, in particular on the effects of pre-culture step, alginate bead size and the physical conditions of culture on the cryopreservation efficiency. Encapsulated and non-pre-cultured gametophytes did not survive cooling with liquid nitrogen. When cryopreservation was preceded by a 2-week period of pre-culture, regrowth reached 42.1%. Reduction in the size of the alginate bead, and culture in total darkness resulted in improved gametophyte regrowth capacity (75.5% or 81.7%, respectively). The best results (91.3%) were obtained when all factors tested occurred simultaneously. The gametophytes recovered very quickly and sporophytes were formed within 4 weeks after rewarming. These simple improvements can be used, not only for the cryopreservation of gametophytes in cryptogams but also for some recalcitrant species of seed plants.
Źródło:: Acta Societatis Botanicorum Poloniae; 2015, 84, 3
0001-6977
2083-9480
Pojawia się w:: Acta Societatis Botanicorum Poloniae
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Changes in cell physiology at the stage of dehydration in a cryopreservation protocol
Autorzy:: Domzalska, L.
Kedracka-Krok, S.
Mikula, A.
Rybczynski, J.J.
Powiązania:: https://bibliotekanauki.pl/articles/951293.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: cryopreservation
cell physiology
dehydration
Gentiana cruciata
proteomic analysis
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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