Temat: identyfikacja gatunkowa - Katalog OPAC zbiorów

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Tytuł:: Metody identyfikacji gatunkowej grzybów z rodzaju Candida. Część I. Techniki fenotypowe
Methods for species identification of genus Candida fungi. Part I. Phenotypic techniques
Autorzy:: Gnat, Sebastian
Powiązania:: https://bibliotekanauki.pl/articles/22323965.pdf
Data publikacji:: 2022
Wydawca:: Krajowa Izba Lekarsko-Weterynaryjna
Tematy:: choroby grzybicze
grzybice
Candida
identyfikacja gatunkowa
metody
metody fenotypowe
czynniki chorobotwórcze
grzyby chorobotwórcze
identification
phenotypic methods
morphology examination
Opis:: The incidence of Candida spp. infections has increased in recent years due to the widespread use of broad-spectrum antibiotics and the growing numbers of immunocompromised individuals. Despite the predominance of Candida albicans, non-albicans Candida species, such as Candida glabrata, Candida tropicalis, Candida guilliermondii, Candida dubliniensis, Candida parapsilosis, and Candida krusei are emerging as both, colonizers and pathogens, that can cause superficial and systemic infections. Currently, there are various methods for identifying yeasts from clinical samples. Conventional methods for identification of Candida species are based on morphological and physiological attributes. Although phenotypic identification methods are cost effective and practical procedures for routine discrimination of isolates in the clinical microbiology laboratory, the accurate identification of all isolates. Moreover, given the variability in the phenotypic characteristics and other irregularities in the taxonomy of the genus Candida, identification based on traditional methods has become less reliable. Important progress has been made with various studies on the phenotypic characteristics of these species using commercially available identification systems. The aim of this review is to summarize the methods available for identification of Candida species. The first part of the article presents the characteristics of phenotypic methods and quick identification techniques, including systems based on the use of chromogenic media.
Źródło:: Życie Weterynaryjne; 2022, 97, 02; 94-104
0137-6810
Pojawia się w:: Życie Weterynaryjne
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Metody identyfikacji gatunkowej grzybów z rodzaju Candida. Część II. Techniki molekularne
Methods for species identification of genus Candida fungi. Part II. Molecular techniques
Autorzy:: Gnat, Sebastian
Powiązania:: https://bibliotekanauki.pl/articles/22326536.pdf
Data publikacji:: 2022
Wydawca:: Krajowa Izba Lekarsko-Weterynaryjna
Tematy:: choroby grzybicze
grzybice
Candida
identyfikacja gatunkowa
metody
metody molekularne
test PCR
metoda multiplex PCR
metoda nested-PCR
metoda real time PCR
fluorescencyjna hybrydyzacja in situ
spektrometria mas MALDI-TOF MS
czynniki chorobotwórcze
grzyby chorobotwórcze
łańcuchowa reakcja polimerazy
piroliza ze spektometrią mas
identification
molecular techniques
MALDI-TOF MS
Opis:: A rapid and accurate identification of the Candida is crucial for clinical treatment of local and systemic candidiasis. Premature diagnosis of invasive fungal infections is problematic because most clinical signs are non-specific and cultures are often negative or become positive too late for the initiation of effective antifungal therapy. Therefore, studies have been performed to improve molecular techniques for the diagnosis of candidiasis. Today, molecular strategies, such as PCR and non-PCR based methods are used to complement conventional laboratory approach. They provide accurate results in much short time of 1.5–3 h. Given the high accuracy and time saving with molecular typing techniques it is likely that most of these methods could improve routine clinical laboratory identification of Candida yeast species. However, further studies are needed for standardization of such procedures. This article presents an overview and discussion on molecular identification methods in the context of their application for the diagnosis of Candida fungi. Particular attention has been focused on the advantages and limitations of the indicated methods and the possibilities of their implementation for routine use in clinical laboratories.
Źródło:: Życie Weterynaryjne; 2022, 97, 03; 167-174
0137-6810
Pojawia się w:: Życie Weterynaryjne
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Metody identyfikacji gatunkowej grzybów z rodzaju Candida. Część III. Systemy automatyczne i testy rzadko wykorzystywane
Methods of species identification of fungi of the genus Candida. Part III. Automated systems and rarely used tests
Autorzy:: Gnat, Sebastian
Powiązania:: https://bibliotekanauki.pl/articles/22354371.pdf
Data publikacji:: 2022
Wydawca:: Krajowa Izba Lekarsko-Weterynaryjna
Tematy:: choroby grzybicze
grzybice
Candida
identyfikacja gatunkowa
metody
systemy automatyczne
system Vitek YBC
system Vitek 2 ID-YST
system Abbott Quantum II
test Tween 80
test wykrywania (1,3)-beta-D-glukanu
test wykrywania galaktomannanu
laboratoria weterynaryjne
czynniki chorobotwórcze
grzyby chorobotwórcze
system identyfikacji drobnoustrojów
Candida fungi
diagnosis
automatic systems
rapid methods
veterinary laboratories
Opis:: It is imperative that pathogenic agents are identified accurately to the species level. The implementation of automatic systems for the identification of Candida yeasts is associated with a large number of tests performed by a given unit, which certainly has a further impact on the price of a single test and the use of devices. Hence, automatic systems are widely used in high-throughput laboratories, e.g. in commercial laboratories with a wide range of activities. Veterinary clinics with their own microbiological unit usually use classical identification methods or semi-automatic systems based on biochemical tests for independent interpretation of the results. Nevertheless, the increased number of invasive procedures, inherent in prolonged hospitalization of animals, and the use of broad-spectrum antibiotic therapy, leads to the conclusion that using automatic fungal identification systems in veterinary laboratories is only a matter of time, and their implementation may leave the classical techniques obsolete. This article provides an overview and discussion of automatic systems as well as new, rapid methods of diagnosing Candida fungi. Particular attention has been focused on Vitek YBC, Vitek 2 ID-YST, Abbot Quantum II, and Microbial ID systems.
Źródło:: Życie Weterynaryjne; 2022, 97, 04; 253-257
0137-6810
Pojawia się w:: Życie Weterynaryjne
Dostawca treści:: Biblioteka Nauki

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