- Tytuł:
- A comparison of PCR-based markers for the molecular identification of Sphagnum species of the section Acutifolia
- Autorzy:
-
Sawicki, J.
Szczecinska, M. - Powiązania:
- https://bibliotekanauki.pl/articles/57748.pdf
- Data publikacji:
- 2011
- Wydawca:
- Polskie Towarzystwo Botaniczne
- Tematy:
-
Acutifolia
random amplified polymorphic DNA
Sphagnum
genetic similarity
molecular identification
molecular marker
polymerase chain reaction
genetic relationship
species identification
peat moss
chloroplast
nuclear genome - Opis:
- RAPDs, ISJs, ISSRs, ITS and katGs were applied to determine genetic relationships between common Sphagnum species of the section Acutifolia. Twenty populations were genotyped using ten ISJ primers, 12 pairs of katG primers, 10 ISSR and 10 RAPD primers, and a restriction analysis of ITS1 and ITS2. ISSR and katG markers revealed the greatest number of species-specific bands. An analysis of ITS1 and ITS2 regions with restriction enzymes also proved to be a highly effective tool for species identification.
- Źródło:
-
Acta Societatis Botanicorum Poloniae; 2011, 80, 3
0001-6977
2083-9480 - Pojawia się w:
- Acta Societatis Botanicorum Poloniae
- Dostawca treści:
- Biblioteka Nauki