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    Wyświetlanie 1-6 z 6
    Tytuł:
    New chitosan wound dressing – first step - the cytotoxicity evaluation
    Autorzy:
    Karuga, Ewa
    Zając, Adam
    Rybak, Zbigniew
    Hanuza, Jerzy
    Powiązania:
    https://bibliotekanauki.pl/articles/1034845.pdf
    Data publikacji:
    2015
    Wydawca:
    Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
    Tematy:
    chitosan
    cytotoxicity
    wound dressing
    Opis:
    The cytotoxicity of chitosan with polyvinox (PCH) and crosslinked chitosan (CH) was studied and analyzed. Cell viability was determined by thiazolyl blue formazan (MTT) assay and cell morphology observations were carried out during cell culturing and MTT tests. Crosslinked chitosan was used as a protective foil (scaffold) for skin wounds. Studies in vitro and the other obtained in this work results prove that the studied materials CH and PCH do not cause cytotoxic activity to Balb 3T3 mouse fibroblasts. CH and PCH are promising biomaterials with prospective application as wound healing dressings.
    Źródło:
    Progress on Chemistry and Application of Chitin and its Derivatives; 2015, 20; 97-109
    1896-5644
    Pojawia się w:
    Progress on Chemistry and Application of Chitin and its Derivatives
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Development of a new production method of foam-like wound dressings for skin regeneration
    Autorzy:
    Vivcharenko, Vladyslav
    Kazimierczak, Paulina
    Wójcik, Michał
    Przekora, Agata
    Powiązania:
    https://bibliotekanauki.pl/articles/284152.pdf
    Data publikacji:
    2019
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    chitosan
    agarose
    biomaterials
    fibroblasts
    cytotoxicity
    Opis:
    Chitosan is widely used to prepare films, hydro-gels, cryogels, sponges, fibers and other various biomaterials used in the tissue engineering field. It is one of the best processable polysaccharides used in biomedicine. However, its stability is generally lower as compared with others, due to its pH sensitivity and hydrophilic character. Using chitosan in combination with agarose may not only improve chemical and mechanical properties of the resultant material (by the formation of a biocomposite), but also lead to the formation of a gel imitating physical attributes of the extracellular matrix. Moreover, the combination of these two polysaccharides has a promising ability to improve the stability of chitosan and to increase fibroblasts’ affinity to agarose. Characteristic advan-tageous features of these natural polymers raise a wide interest in tissue engineering. The aim of this study was to develop and optimize a new method to produce a highly biocompatible foam-like chitosan/agarose wound dressing for skin healing applications. The production process optimization helped to obtain the absorbent foam-like biomaterial which is non-toxic to skin fibroblasts and does not conduce their adhesion. Employing sodium bicarbonate as the main agent in the foaming reaction not only led to obtaining the foam-like structure but also neutralized the acidic pH, making the material non-toxic and non-irritating to the skin. In conclusion, the new foam-like biomaterial has great potential for biomedical applications as the wound dressing accelerating the healing process of the damaged tissues.
    Źródło:
    Engineering of Biomaterials; 2019, 22, 152; 16-20
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Physicochemical properties and cytotoxicity of hydrogels based on Beetosan® containing sage and bee pollen
    Autorzy:
    Tyliszczak, Bożena
    Drabczyk, Anna
    Kudłacik-Kramarczyk, Sonia
    Grabowska, Beata
    Kędzierska, Magdalena
    Powiązania:
    https://bibliotekanauki.pl/articles/1038565.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    hydrogels
    cytotoxicity
    fibroblasts
    chitosan
    sage
    wettability
    Opis:
    Currently, increasing attention is being paid to issues related to environmental protection, waste management, as well as to the development of polymers with useful properties. The research presented here involved preparation of hydrogels based on Beetosan® - a chitosan derived from the multi-stage processing of dead bees. Moreover, hydrogels were additionally modified with natural substances - i.e. bee pollen and extract of Salvia officinalis (sage) that are well known for the presence of many compounds with beneficial properties from a medical point of view. Materials have been first obtained by photopolymerization. Then, their surface morphology, wettability and cytotoxicity to selected cell lines have been determined. It can be stated that such combination of Beetosan® hydrogel matrix and the mentioned additives resulted in a preparation of polymers characterized by negative impact on cancer cells. Impact of hydrogels with sage is slightly more intense due to the presence of substances such as ursalic or rosmaric acid that are characterized to have anticancer activity. Such negative impact has not been observed in case of studies using fibroblasts. Furthermore, addition of natural substances into hydrogels resulted in a more homogeneous surface and in the decrease of wettability angle of the tested polymers. It can be concluded that the use of natural-derived reagents and synthesis of polymers using these reagents (as a result of environmentally friendly photopolymerization) yields materials with interesting properties for medical purposes, with particular emphasis on antitumor activity, and without significant negative impact on fibroblasts.
    Źródło:
    Acta Biochimica Polonica; 2017, 64, 4; 709-712
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Structural and biological characteristics of self-organising chitosan hydrogels
    Autorzy:
    Pieklarz, Katarzyna
    Tylman, Michał
    Modrzejewska, Zofia
    Galita, Grzegorz
    Majsterek, Ireneusz
    Powiązania:
    https://bibliotekanauki.pl/articles/2131528.pdf
    Data publikacji:
    2021
    Wydawca:
    Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
    Tematy:
    biomaterials
    chitosan
    cytotoxicity
    self-organising hydrogels
    structural properties
    tissue engineering
    Opis:
    Creating innovative methods of treatment and regeneration of damaged tissues or organs is a key challenge of the twenty-first century. The aim of this study was to determine the possibility of producing and characterising the properties of self-organising chitosan hydrogels prepared with the use of chitosan lactate/chloride and disodium hydrogen phosphate dodecahydrate as a cross-linking agent. The structure and supramolecular architecture of the biomaterials were evaluated by Fourier-transform infrared spectroscopy and polarised optical microscopy. Biological studies assessed cytotoxicity by contact with a human colon adenocarcinoma cell line. The colourimetric resazurin assay showed that the obtained chitosan hydrogels are non-cytotoxic materials. Thus, self-organising biomaterials hold great promise for application in tissue engineering.
    Źródło:
    Progress on Chemistry and Application of Chitin and its Derivatives; 2021, 26; 191-199
    1896-5644
    Pojawia się w:
    Progress on Chemistry and Application of Chitin and its Derivatives
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ocena struktury, modułu Younga oraz cytotoksyczności kompozytów na bazie chitozanu
    Structural properties, Youngs modulus and cytotoxicity assessment of chitosan-based composites
    Autorzy:
    Przekora, A.
    Pałka, K.
    Macherzyńska, B.
    Ginalska, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/283835.pdf
    Data publikacji:
    2012
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    chitozan
    rusztowanie
    struktura
    moduł Younga
    cytotoksyczność
    chitosan
    scaffold
    structure
    Young's modulus
    cytotoxicity
    Opis:
    Ceramika wapniowo-fosforanowa w formie porowatego rusztowania na bazie biodegradowalnego biopolimeru jest materiałem powszechnie stosowanym jako wypełniacz ubytków tkanki kostnej w ortopedii i stomatologii. Chitozan, ze względu na swoje charakterystyczne właściwości takie jak: podobieństwo strukturalne do glikozaminoglikanów (GAG) macierzy pozakomórkowej tkanki kostnej, brak toksyczności, szybka biodegradacja, podatność na chemiczną i enzymatyczną modyfikację, stymulacja adhezji i proliferacji komórek oraz osteoinduktywność, jest często stosowany w inżynierii tkankowej kości jako składnik kompozytów. W niniejszej pracy, określono parametry strukturalne i mechaniczne oraz cytotoksyczność typów kompozytów na bazie chitozanu (chitozan-HA BIOCER oraz chitozan-HT BIOCER). Testy in vitro przeprowadzono z wykorzystaniem linii komórkowej hFOB 1.19 (ludzkie płodowe osteoblasty). Cytotoksyczność ekstraktów z kompozytów oznaczono za pomocą testu LDH. Wyniki badań wyraźnie wskazują, że wyprodukowane kompozyty na bazie krylowego chitozanu wykazują dobre parametry strukturalne i mechaniczne o dużej zgodności z tkankami, są nietoksyczne i przez to są obiecującym materiałem do stosowania w inżynierii tkankowej kości.
    Calcium phosphate ceramics in the form of biodegradable biopolymer-based, porous scaffolds are widely used as bone defect filler in dentistry and orthopedics. Chitosan is often applied in bone tissue engineering as a component of composites because of its characteristic properties such as structural similarity to glycosaminoglycans (GAG) of bone extracellular matrix, nontoxicity, rapid biodegradation, prone to chemical and enzymatic modification, stimulation of cell adhesion and proliferation and osteoinduction. In this work, the structure, mechanical properties and cytotoxicity of 2 types of chitosan-based composites (chitosan-HA BIOCER and chitosan-HT BIOCER) were evaluated. In vitro cell culture tests were carried out using hFOB 1.19 cell line (human fetal osteoblast cells). The cytotoxicity of scaffolds extracts was estimated by LDH test. Our studies clearly indicate that created krill chitosan-based composites show good structural and mechanical properties with good compatibility with human tissues. Furthermore, produced composites are nontoxic and thus are promising materials for bone tissue engineering application.
    Źródło:
    Engineering of Biomaterials; 2012, 15, 114; 52-58
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cell binding and penetration of quaternized chitosan derivatives
    Autorzy:
    Zubareva, Anastasia A.
    Shagdarova, Balzhima Ts.
    Varlamov, Valery P.
    Svirshchevskaya, Elena V.
    Powiązania:
    https://bibliotekanauki.pl/articles/1034738.pdf
    Data publikacji:
    2016
    Wydawca:
    Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
    Tematy:
    N-[(2-hydroxy-3-trimethylammonium) propyl] chitosan chloride
    cell uptake
    chitosan
    cytotoxicity
    quaternized chitosan
    trypan blue
    Opis:
    Chitosan (Ch) is an attractive biopolymer with multiple reactive groups. However it is poorly soluble at neutral pH. Quaternization improves its solubility and permits the development of various positively charged drug delivery systems. The aim of this work was to study the solubility, toxicity, cell binding, and penetration of 20 kDa chitosan with 9, 40, 58 and 98% of quaternary ammonium group substitution (ChQ1 to ChQ4 accordingly). We showed that ChQ with substitution degree >40% was soluble in a wide pH range. Unexpectedly ChQ2 and ChQ3 were more toxic to cells than Ch, ChQ1 and ChQ4. Higher toxicity of ChQ was found against macrophage like cell line RAW264.7 than against epithelial cells MiaPaCa-2. All ChQ, in contrast to unmodified Ch, easily bound and penetrated the cells with the highest uptake by ChQ4. Thus, quaternized chitosan derivatives can be used for biomedical applications.
    Źródło:
    Progress on Chemistry and Application of Chitin and its Derivatives; 2016, 21; 217-223
    1896-5644
    Pojawia się w:
    Progress on Chemistry and Application of Chitin and its Derivatives
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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