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Tytuł:
Structural and biological characteristics of self-organising chitosan hydrogels
Autorzy:
Pieklarz, Katarzyna
Tylman, Michał
Modrzejewska, Zofia
Galita, Grzegorz
Majsterek, Ireneusz
Powiązania:
https://bibliotekanauki.pl/articles/2131528.pdf
Data publikacji:
2021
Wydawca:
Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
Tematy:
biomaterials
chitosan
cytotoxicity
self-organising hydrogels
structural properties
tissue engineering
Opis:
Creating innovative methods of treatment and regeneration of damaged tissues or organs is a key challenge of the twenty-first century. The aim of this study was to determine the possibility of producing and characterising the properties of self-organising chitosan hydrogels prepared with the use of chitosan lactate/chloride and disodium hydrogen phosphate dodecahydrate as a cross-linking agent. The structure and supramolecular architecture of the biomaterials were evaluated by Fourier-transform infrared spectroscopy and polarised optical microscopy. Biological studies assessed cytotoxicity by contact with a human colon adenocarcinoma cell line. The colourimetric resazurin assay showed that the obtained chitosan hydrogels are non-cytotoxic materials. Thus, self-organising biomaterials hold great promise for application in tissue engineering.
Źródło:
Progress on Chemistry and Application of Chitin and its Derivatives; 2021, 26; 191-199
1896-5644
Pojawia się w:
Progress on Chemistry and Application of Chitin and its Derivatives
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chitosan hydrogels modified with silk, graphene oxide or reduced graphene oxide
Autorzy:
Szaraniec, Barbara
Boruchowski, Marcin
Gryń, Karol
Chłopek, Jan
Powiązania:
https://bibliotekanauki.pl/articles/1844967.pdf
Data publikacji:
2020
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
hydrogels
nanocomposites
chitosan
hydrożele
nanokompozyty
Źródło:
Engineering of Biomaterials; 2020, 23, 158 spec. iss.; 44
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chitosan hydrogels modified with silk, graphene oxide or reduced graphene oxide
Autorzy:
Szaraniec, Barbara
Boruchowski, Marcin
Gryń, Karol
Chłopek, Jan
Powiązania:
https://bibliotekanauki.pl/articles/1844881.pdf
Data publikacji:
2020
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
hydrogels
nanocomposites
chitosan
hydrożele
nanokompozyty
chitozan
Źródło:
Engineering of Biomaterials; 2020, 23, 158 spec. iss.; 44
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis of chitosan and carboxymethyl chitosan hydrogels by electron beam irradiation
Autorzy:
Czechowska-Biskup, Renata
Wach, Radosław
Stojek, Piotr
Kamińska, Martyna
Rosiak, Janusz M.
Ulański, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/1034681.pdf
Data publikacji:
2016
Wydawca:
Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
Tematy:
PEGDA
carboxymethyl chitosan
chitosan
hydrogel
radiation crosslinking
Opis:
Solutions of chitosan (CS) and carboxymethyl chitosan (CMCS) were subjected to irradiation by electron beam in presence of a crosslinking agent (poly(ethylene glycol) diacrylate - PEGDA) in order to produce carboxymethyl chitosan- and chitosan-based hydrogels. PEGDA macromonomer itself undergoes simultaneous polymerization and crosslinking either in neutral water or in acidic medium. Acidic solutions of chitosan of 0.5, 1 and 2% can be effectively crosslinked with PEGDA to form a gel. Although CMCS undergoes radiation-initiated crosslinking only at high concentration in water (over 10%), the presence of PEGDA in solution facilitated hydrogel formation even at lower concentration of CMCS. Formation of CS and CMCS hydrogels required irradiation doses lower than those needed for sterilization, i.e. 25 kGy, in some cases even as low as 200 Gy. Sol-gel analysis revealed relatively high gel fraction of obtained hydrogels, up to 80%, and good swelling ability. Both parameters can be easily controlled by composition of the initial solution and irradiation dose. Possible mechanisms of crosslinking reactions were proposed, involving addition of the polysaccharide macroradicals to a terminal double bond of PEGDA.
Źródło:
Progress on Chemistry and Application of Chitin and its Derivatives; 2016, 21; 27-45
1896-5644
Pojawia się w:
Progress on Chemistry and Application of Chitin and its Derivatives
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Fabrication and characterization of graphene-loaded chitosan hydrogels with cross-linking gradient
Autorzy:
Kosowska, K.
Domalik-Pyzik, P.
Chłopek, J.
Powiązania:
https://bibliotekanauki.pl/articles/285566.pdf
Data publikacji:
2018
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
hydrogels
chitosan
nanocomposites
Źródło:
Engineering of Biomaterials; 2018, 21, 148; 40
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Preparation and characterization of a new generation of chitosan hydrogels containing pyrimidine ribonucleotides
Autorzy:
Pieklarz, Katarzyna
Tylman, Michał
Modrzejewska, Zofia
Powiązania:
https://bibliotekanauki.pl/articles/1034023.pdf
Data publikacji:
2020
Wydawca:
Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
Tematy:
biomaterial
chitosan
hydrogel
neural tissue regeneration
Opis:
Damage to the nervous system, in particular spinal cord injuries, is a burden for the patient and is usually the cause of irreversible disability. The progress observed in the last decade in the fields of biology, biomaterial engineering and neurosurgery has created new treatment solutions while preventing further neurodegenerative processes. The most important research is focused on the implementation of polymer structures in clinical practice, especially chitosan hydrogels, which are the scaffolds for regenerating axons. This article presents a new generation of biomaterials that have the ability to gel in response to temperature changes; they are intended for injectable scaffolds for nerve cell cultures. Two types of hydrogels were prepared based on chitosan lactate and chitosan chloride using uridine 5’-monophosphate disodium salt. The structure of the systems was observed under a scanning electron microscope and examined using Fourier transform infrared spectroscopy. In addition, thermal properties were tested using differential scanning calorimetry.
Źródło:
Progress on Chemistry and Application of Chitin and its Derivatives; 2020, 25; 192-200
1896-5644
Pojawia się w:
Progress on Chemistry and Application of Chitin and its Derivatives
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Acceleration of gelation and promotion of mineralization of chitosan hydrogels by alkaline phosphatase
Autorzy:
Douglas, T. E. L.
Skwarczyńska, A.
Modrzejewska, Z.
Balcaen, L.
Schaubroeck, D.
Lycke, S.
Vanhaecke, F.
Vandenabeele, P.
Dubruel, P.
Jansen, J. A.
Leeuwenburgh, S. C. G.
Powiązania:
https://bibliotekanauki.pl/articles/284363.pdf
Data publikacji:
2012
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
chitosan
thermosensitivity
composite
mineralization
Opis:
Thermosensitive chitosan hydrogels containing sodium beta-glycerophosphate (ß-GP), whose gelation is induced by increasing temperature to body temperature, were functionalized by incorporation of Alkaline Phosphatase (ALP), an enzyme involved in mineralization of bone. ALP incorporation led to acceleration of gelation upon increase of temperature for four different chitosan preparations of differing molecular weight, as demonstrated by rheometric time sweeps at 37°C. Hydrogels containing ALP were subsequently incubated in calcium glycerophosphate (Ca-GP) solution to induce their mineralization with calcium phosphate (CaP) in order to improve their suitability as materials for bone replacement. Incorporated ALP retained its bioactivity and induced formation of CaP mineral, as confirmed by SEM, FTIR, Raman spectroscopy, XRD, ICP-OES, and increases in dry mass percentage, which rose with increasing ALP concentration and incubation time in Ca-GP solution. The results demonstrate that ALP accelerates formation of thermosensitive chitosan/ß-GP hydrogels and induces their mineralization with CaP, which paves the way for applications as injectable bone replacement materials.
Źródło:
Engineering of Biomaterials; 2012, 15, no. 116-117 spec. iss.; 135-136
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of graphene oxide-loaded chitosan hydrogels and their application for 3D printing
Autorzy:
Domalik-Pyzik, P.
Chłopek, J.
Powiązania:
https://bibliotekanauki.pl/articles/286115.pdf
Data publikacji:
2017
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
hydrogels
graphene
chitosan
Źródło:
Engineering of Biomaterials; 2017, 20, no. 143 spec. iss.; 83
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characteristics of physicochemical and rheological properties of chitosan hydrogels based on selected hydroxy acids
Autorzy:
Zavyalova, Olga
Gajewska, Sandra
Dąbrowska-Wisłocka, Dominika
Sionkowska, Alina
Powiązania:
https://bibliotekanauki.pl/articles/2058389.pdf
Data publikacji:
2021
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
chitosan
lactobionic acid
mandelic acid
rheology
FTIR spectra
chitozan
reologia
hydrożele
Opis:
Chitosan is a natural cationic polymer that dissolves in an acidic environment and forms gels. Its properties depend on the degree of deacetylation and molecular weight. It is a bioactive compound with antibacterial and film-forming properties that allow to increase the regenerative capacity of the skin. Moreover, it is biodegradable, biocompatible, non-toxic, and stable. In this research, chitosan was combined with mandelic and lactobionic acids which are characterized by biological activity and low toxicity. This combination not only has a positive effect on the chitosan solubility, but it also allows to obtain new biomaterials whose positive features of the base ingredients are enhanced by their synergistic effect. The obtained hydrogels were assessed regarding the interaction of chitosan and hydroxy acid molecules, and the stability of the resulting structures was examined. The research was performed by using rheological methods and IR spectroscopy. Chitosan hydrogels made with mandelic acid are characterized by higher viscosity values, as compared to hydrogels containing lactobionic acid. The samples of the obtained hydrogels stored for 7 days showed no signs of degradation and their viscosity values were constantly increasing, which proves the ongoing process of creating new bonds between hydroxy acid molecules and chitosan chains. After this time, the hydrogels with mandelic acid revealed higher viscosity values in comparison to hydrogels made with lactobionic acid. Based on the obtained IR spectra, the shifts of the characteristic chitosan bands as a result of interaction with the tested hydroxy acids were analyzed.
Źródło:
Engineering of Biomaterials; 2021, 24, 161; 2--7
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of texture profile analysis to investigate the mechanical properties of thermosensitive injectable chitosan hydrogels
Autorzy:
Owczarz, Piotr
Rył, Anna
Wichłacz, Żaneta
Powiązania:
https://bibliotekanauki.pl/articles/1034254.pdf
Data publikacji:
2019
Wydawca:
Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
Tematy:
TPA
chitosan
hydrogels
injectable scaffolds
mechanical properties
Opis:
In this paper, the mechanical properties of hybrid chitosan hydrogels were investigated using the texture profile analysis test. Scaffolds obtained from a polysaccharide of various molecular weights were studied, which, with the addition of glycerophosphate salt, formed a three-dimensional structure in vivo. The obtained systems were also enriched with collagen and calcium carbonate to improve the mechanical properties. The determined texture parameter values indicate that the mechanical properties of the hybrid hydrogel depend on the molecular weight of the polymer, the type of solvent and, the pH-neutralizing substance, as well as the type and concentration of the filler. Moreover, in some cases the TPA test was the only way to evaluate the mechanical properties of the obtained hydrogels due to the inability to determine the Young's modulus. Consequently, the texture analysis test is a valuable tool for selecting solutions depending on the intended application of the scaffolds.
Źródło:
Progress on Chemistry and Application of Chitin and its Derivatives; 2019, 24; 151-163
1896-5644
Pojawia się w:
Progress on Chemistry and Application of Chitin and its Derivatives
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Structural characteristics of thermosensitive chitosan glutaminate hydrogels
Autorzy:
Modrzejewska, Zofia
Nawrotek, Katarzyna
Zarzycki, R
Douglas, Timothz
Powiązania:
https://bibliotekanauki.pl/articles/1035319.pdf
Data publikacji:
2013
Wydawca:
Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
Tematy:
chitosan
hydrogel
natural polymer
structural properties
Opis:
Hydrogels that possess the ability of gelling in response to changes in the local environment, such as pH or temperature, have been examined extensively recently. In this paper the properties of thermosensitive chitosan hydrogels prepared with the use of chitosan glutaminate and -glycerophosphate are presented. The sol/gel transition point was determined based on the rheological properties. The structure of gels was observed under the Scanning Electron Microscopy (SEM) and was investigated by thermogravimetric (TG) and differential themogravimetric (DTG) analy¬sis and infrared (IR) spectroscopy. The crystallinity of gel structure was determined by X-ray Diffraction analysis (XRD).
Źródło:
Progress on Chemistry and Application of Chitin and its Derivatives; 2013, 18, 18; 93-105
1896-5644
Pojawia się w:
Progress on Chemistry and Application of Chitin and its Derivatives
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ promieniowania mikrofalowego oraz UV na właściwości hydrożeli chitozanowych
Influence of microwave and UV radiation on properties of chitosan hydrogels
Autorzy:
Bialik-Wąs, K.
Kudłacik, S.
Drabczyk, A.
Tyliszczak, B.
Powiązania:
https://bibliotekanauki.pl/articles/101311.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Stowarzyszenie Infrastruktura i Ekologia Terenów Wiejskich PAN
Tematy:
hydrożele
chitozan
Beetosan
promieniowanie UV
promieniowanie mikrofalowe
hydrogels
chitosan
UV radiation
microwave radiation
Opis:
W prezentowanym artykule porównano wpływ promieniowania mikrofalowego oraz UV na przebieg reakcji sieciowania hydrożeli chitozanowych. Syntezy prowadzono zarówno w oparciu o chitozan komercyjny, jak i Beetosan - chitozan pochodzący z pszczół z osypu zimowego oraz dla układów mieszanych chitozan - Beetosan. Otrzymane materiały hydrożelowe zostały poddane badaniom zdolności pęcznienia i badaniom inkubacyjnym w wodzie destylowanej oraz symulowanych płynach ustrojowych. Ponadto, dla wybranych układów określono morfologię powierzchni przy użyciu skaningowego mikroskopu elektronowego. Wyznaczono również kąty zwilżania θ metodą geometryczną poprzez naniesienie kropli cieczy na powierzchnię badanego materiału.
In this paper, the impact on microwave radiation and UV cross-linking of the course of reaction of obtaining chitosan hydrogels was presented. The synthesis were conducted using either commercial chitosan and Beetosan - chitosan derived from naturally died honeybees as well as using mixed systems containing both chitosan and Beetosan. The swelling capacity of obtained hydrogel materials as well as their tendency to degradation in distilled water and simulated body fluid have been tested. Moreover, morphology of the surface of selected samples using scanning electron microscope was characterized. Furthermore, the contact angles (θ) of attained materials were defined using geometrical method by applying a drop of liquid on the surface of the tested material.
Źródło:
Infrastruktura i Ekologia Terenów Wiejskich; 2016, II/2; 605-614
1732-5587
Pojawia się w:
Infrastruktura i Ekologia Terenów Wiejskich
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis of bees’ chitosan based hydrogels containing Aloe vera
Autorzy:
Kudłacik-Kramarczyk, S.
Drabczyk, A.
Tyliszczak, B.
Powiązania:
https://bibliotekanauki.pl/articles/101614.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Stowarzyszenie Infrastruktura i Ekologia Terenów Wiejskich PAN
Tematy:
beetosan
chitosan
hydrogels
Aloe vera
bees
waste management
Opis:
Chitosan based hydrogels due to their specific properties and structure belong to the group of materials widely used in medicine. They are used as drug carriers with controlled release of active substances and in the preparation of innovative wound dressings. The main aim of the conducted research was the choice of the most favorable parameters for carrying the cross-linking reaction by means of UV radiation and microwaves. Subsequently, an adequate composition of the mixture containing chitosan and gelatin for further modification of such prepared the polymer matrix with Aloe vera extract was selected. Subsequently, an impact of introduction of Aloe vera extract into hydrogel on its physicochemical properties and structure was determined. The scope of the research included the measurement of swelling abilities of hydrogels in distilled water and selected simulated body fluids as well as incubation studies aimed at determination of tendency of prepared materials to degradation in previously mentioned fluids and their compatibility in relation to such environments. In order to characterize the chemical structure of the polymers and impact of incubation on this structure spectroscopic analysis was performed. Microscopic analysis was used to define the surface morphology of the hydrogels. Moreover, materials containing Aloe vera extract were subjected to the studies of release of additive from hydrogel matrix. Research was conducted both in acid and alkaline environment.
Źródło:
Infrastruktura i Ekologia Terenów Wiejskich; 2017, IV/2; 1687-1698
1732-5587
Pojawia się w:
Infrastruktura i Ekologia Terenów Wiejskich
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ substancji czynnych na własności reologiczne hydrożeli chitozanowych w pobliżu granicy przejścia fazowego zol-żel
Influence of active substances on rheological properties of chitosan hydrogels near sol-gel phase transition
Autorzy:
Owczarz, P.
Modrzejewska, Z.
Powiązania:
https://bibliotekanauki.pl/articles/2071912.pdf
Data publikacji:
2012
Wydawca:
Stowarzyszenie Inżynierów i Techników Mechaników Polskich
Tematy:
chitozan
hydrożele
reologia hydrożeli
matryce polimerowe
chitosan
hydrogel
rheology of hydrogels
polymeric network
Opis:
W pracy przedstawiono wyniki badań Teologicznych hydrożeli chitozanowych tworzących matrycę polimerową dla substancji czynnych. Jako substancje czynne stosowano albuminę wołową oraz wankomycynę. Inhibitorem przejścia fazowego zol-żel była zmiana temperatury roztworu. Wyznaczone własności reologiczne oraz krzywe temperaturowe roztworów wykazały, że w przypadku albuminy otrzymany roztwór zachowuje podobne własności jak wyjściowy roztwór chitozanu. W przypadku wankomycyny zainicjowany zmianą temperatury proces żelowania prowadzi do wytworzenia nieodwracalnej struktury.
Rheological properties of chitosan hydrogels forming polymeric network for active substances are presented in the paper. Bovine serum albumin and vancomycin were used as active substances. A change in solution tem-perature acted as an inhibitor of sol-gel phase transition. The determined rheological properties and temperature curves of solutions showed properties similar to those of initial chitosan solution. In case of vancomycin the gelation process initiated by a temperature change led to the formation of irreversible structure.
Źródło:
Inżynieria i Aparatura Chemiczna; 2012, 6; 364-365
0368-0827
Pojawia się w:
Inżynieria i Aparatura Chemiczna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Double crosslinking of chitosan/vanillin hydrogels as a basis for mechanically strong gradient scaffolds for tissue engineering
Autorzy:
Hunger, Martyna
Domalik-Pyzik, Patrycja
Reczyńska, Katarzyna
Chłopek, Jan
Powiązania:
https://bibliotekanauki.pl/articles/1844976.pdf
Data publikacji:
2020
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
hydrogel
chitosan
vanillin
tripolyphosphate
tissue engineering
Opis:
Polysaccharides, such as chitosan (CS), are widely used in many biomedical applications. However, they require crosslinking agents to achieve chemical stability and appropriate mechanical properties. In this work, chitosan-based hydrogels were crosslinked using vanillin and/or sodium tripolyphosphate, as chemical and physical crosslinking agents, respectively. Microstructural (digital microscope, SEM), structural (FTIR-ATR), mechanical (static compression test), and in vitro biological (chemical stability and swelling ratio in PBS, cytotoxicity) properties of the obtained materials were evaluated to assess materials potential as biomedical scaffolds. The optimal ratio of vanillin to chitosan (DD = 89%) to crosslink the polymer was found to be 1.2:1. Moreover, the double crosslinking with vanillin caused a two-time increase in the compression strength of the samples and led to the slower biodegradability. Cytotoxicity studies showed that the cells prefer double vanillin crosslinked hydrogels over those treated with TPP. Further studies, such as bioactivity are required to determine the specific functionality of the hydrogels and the specific tissue which may be treated with the tested materials. The optimal material was chosen to the next step of the study, which may be obtaining composite hydrogels with hydroxyapatite and/or graphene oxide to tailor or improve properties towards specific tissue regeneration.
Źródło:
Engineering of Biomaterials; 2020, 23, 155; 2-11
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
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