Temat: Proliferation - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: CAMPTOTHECIN INHIBITS MIGRATION, INVASION AND CLONOGENIC PROPERTY OF LIVER CANCER CELLS BY MODULATING MICRORNA EXPRESSION
Autorzy:: Liu, Zhenzhong
Wu, Song
Li, Xiaoqian
Powiązania:: https://bibliotekanauki.pl/articles/895460.pdf
Data publikacji:: 2020-04-29
Wydawca:: Polskie Towarzystwo Farmaceutyczne
Tematy:: Hepatocellular Carcinoma
cell proliferation
cell cycle
Camptothecin
miRNA
Opis:: Camptothecin (CPT), an alkaloid natural product, extracted from Camptotheca acuminata bark, has been reported to have potential antitumor activity in diverse cancers. MicroRNAs (MiRNAs) are a class of short, non-coding RNAs that plays a crucial role in the normal physiology by attenuating translation. Here, we showed that the CPT modulates the expression of miRNAs in hepatocellular carcinoma cells (HCC). Microarray analysis reveals that CPT modulates the expression of as many as 39 miRNAs in HCC cells (Huh7), 27 miRNAs were downregulated whereas 12 miRNAs were upregulated. miR-16 is the key miRNA upregulated by CPT and targets key prosurvival proteins (MMP-2, MMP-9 and cyclin D1). Our results demonstrate that CPT is inhibiting cell viability of HCC cells significantly when compared with the untreated cells. Wound healing and colony formation assay confirm inhibition of cell migration and clonogenic property of Huh7 cells respectively, upon the dose-dependent treatment of CPT. Furthermore, the Boyden chamber assay analysis revealed a significant inhibition of number of invasive cells in CPT treated cells with comparison to untreated Huh7 cells. Mechanistically, CPT upregulates miR-16 expression which targets MMP-2, MMP-9, cyclin D1 downregulation and subsequently upregulates the expression of E-cadherin, TIMP1, p21, and p27, thereby inhibits cell migration, invasion and clonogenic property of HCC cells. In summary, CPT treatment in Huh7 cells decreases cell viability and upregulates miR-16 expression, which results in inhibition of cell migration, invasion and clonogenic property of cells, by decreasing MMP-2, MMP-9, cyclin D1 and increasing the expression of cell cycle-regulated proteins p21 and p27.
Źródło:: Acta Poloniae Pharmaceutica - Drug Research; 2020, 77, 2; 295-304
0001-6837
2353-5288
Pojawia się w:: Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: The effect of indole-3-carbinol on the expression of CYP1A1, CYP1B1 and AhR genes and proliferation of MCF-7 cells
Autorzy:: Ociepa-Zawal, Marta
Rubiś, Błażej
Łaciński, Mariusz
Trzeciak, Wiesław
Powiązania:: https://bibliotekanauki.pl/articles/1041121.pdf
Data publikacji:: 2007
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: p21
AhR
CYP
cell proliferation
estrone hydroxylation
xenoestrogens
Opis:: The influence of an antiestrogen, indole-3-carbinol (I3C) on the expression of CYP1A1, CYP1B1 and AhR genes was investigated in an attempt to establish whether I3C could increase the expression of genes involved in estrone metabolism. Another purpose was to examine the proliferation of an estrogen-dependent breast cancer cell (MCF-7 line) under the influence of I3C and both I3C and DDT. In MCF-7 cells incubated with I3C or I3C and DDT combined, quantitative RT-PCR analysis revealed a significant increase in the level of CYP1A1, AhR, and CYP1B1 transcripts. The proliferation rate of MCF-7 cells was increased by treatment with DDT or estradiol (E2), whereas I3C did not affect the proliferation of MCF-7 cells but greatly reduced the stimulatory effect of DDT, and abolished the effect of E2. The level of p21 transcript, encoding p21 protein involved in the cell cycle, was increased several-fold by I3C comparing to its level in cells incubated with estradiol or DDT. The results suggest that the proliferation of MCF-7 cells is accompanied not only by expression of genes encoding cytochromes involved in estrogen metabolism, but also by changes in the expression of other genes including that encoding p21 protein involved in the cell cycle.
Źródło:: Acta Biochimica Polonica; 2007, 54, 1; 113-117
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Elevated expression of serine/threonine phosphatase type 5 correlates with malignant proliferation in human osteosarcoma
Autorzy:: Han, Kun
Gan, Zhihua
Lin, Shuchen
Hu, Haiyan
Shen, Zan
Min, Daliu
Powiązania:: https://bibliotekanauki.pl/articles/1038676.pdf
Data publikacji:: 2017
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: cell proliferation
lentivirus
Osteosarcoma
PP5
siRNA
targeted therapy
Opis:: Osteosarcoma is the most common primary malignant bone tumor in adolescents and young adults. However, the involvement of serine/threonine phosphatase type 5 (PP5) in osteosarcoma remains unclear. The aim of this study was to evaluate the functional role of PP5 in osteosarcoma cells. Firstly, we found that PP5 is widely expressed in several human osteosarcoma cell lines. Then we used lentivirus-delivered siRNA to silence PP5 expression in Saos-2 and U2OS cell lines. Knockdown of endogenous PP5 expression by shRNA-expressing lentivirus significantly decreased the viability and proliferation of the osteosarcoma cells. Moreover, FACS analysis showed that knockdown of PP5 expression induced a significant arrest in the G0/G1 phase of the cell cycle, which was associated with the inhibition of cell proliferation. Therefore, knockdown of PP5 is likely to provide a novel alternative to targeted therapy of osteosarcoma and deserves further investigation.
Źródło:: Acta Biochimica Polonica; 2017, 64, 1; 11-16
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Clastogenic and toxicological assessment of cashew (Anacardium occidentale) nut bark extracts in Wistar rats
Autorzy:: Owumi, Solomon
Fatoki, John
Gbadegesin, Michael
Odunola, Oyeronke
Powiązania:: https://bibliotekanauki.pl/articles/1039006.pdf
Data publikacji:: 2015
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: Cashew bark oil
tattooing
toxicity
micronuclei
cell proliferation
skin cancer
Opis:: Occupational exposures to environmental toxicants have been associated with the onset of skin lesions-including cancers. Identification and reduction of exposure to such compounds is an important public health goal. We examined the effect of cashew shell oil (CSO), used in skin tattooing for its potential to induce skin transformation in rats. Corn oil and CSO (25, 50, and 100%) were topically applied to depilated sections of Wistar' rat skin (groups: I-IV) for six weeks. Effect of treatments on serum transaminases activity, histological changes in hepatocytes and induction of micronuclei in the bone marrow were examined. In addition, CSO-induced hepatocyte proliferation was also quantified. All animals survived the course of the study. Reduced percentage change in body weight and physical trauma were observed in CSO-treated rat. The effects were more prominent in Group IV (100% CSO). Relative liver weights and number of hepatocytes (cells/mm2) increased significantly in groups II-IV relative to control (p < 0.05). Serum transaminases activities were not significantly (p > 0.05) affected in treated groups. Hepatic histopathology revealed moderate sinusoidal congestion (group II), in addition to portal congestion in (group III), with mononuclear cellular infiltration (group IV) animals. In addition, CSO induced significant micronuclei formation of polychromatic erythrocyte (mPCEs) in the rat bone marrow (p < 0.05) when compared with control. Topical application of CSO disrupted skin cells integrity resulting in physical trauma. In addition, CSO appears to be clastogenic and induces hepatocyte proliferation. Occupational exposure to CSO especially for engraving tattoos in humans should be discouraged and further studies need to be conducted.
Źródło:: Acta Biochimica Polonica; 2015, 62, 3; 563-567
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Molecular cloning, expression, purification and osteoblasts proliferation activity of sika deer thymosin beta10
Autorzy:: Zhao, D.
Zhao, Y.
Zhang, H.
Xu, Z.
Liu, M.
Wang, J.
Bai, X.
Powiązania:: https://bibliotekanauki.pl/articles/2087874.pdf
Data publikacji:: 2017
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: affinity chromatography
cell proliferation
prokaryotic expression
sika deer
thymosin 10
Źródło:: Polish Journal of Veterinary Sciences; 2017, 4; 751-758
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Overexpression of human CUTA isoform2 enhances the cytotoxicity of copper to HeLa cells
Autorzy:: Yang, Jingchun
Li, Qiang
Yang, Huirong
Yan, Lichong
Yang, Liu
Yu, Long
Powiązania:: https://bibliotekanauki.pl/articles/1040764.pdf
Data publikacji:: 2008
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: copper toxicity
CUTA isoform2
overexpression
copper-induced apoptosis
cell proliferation
Opis:: Copper, an essential transient element, can be toxic to cells when present in excess. Altered copper homeostasis is involved in pathological events of many diseases. Human CUTA isoform2 is a member of cation tolerance protein (CutA1) family. In this study, we examined the effect of CUTA isoform2 overexpression on copper toxicity. It was shown that overexpressed CUTA isoform2 sensitized HeLa cells to copper toxicity by promoting copper-induced apoptosis. The inhibition effect of excessive copper on cell proliferation was also enhanced by overexpressed CUTA isoform2. So CUTA isoform2 was implicated to be involved in the cytotoxicity of copper.
Źródło:: Acta Biochimica Polonica; 2008, 55, 2; 411-415
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Glutathione-dependent regulation of cell proliferation and root meristem development
Autorzy:: Schnaubelt, D.
Queval, G.
Dong, Y.
Diaz-Vivancos, P.
Hannah, M.
Foyer, C.
Powiązania:: https://bibliotekanauki.pl/articles/79874.pdf
Data publikacji:: 2013
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: conference
cell proliferation
root meristem
Arabidopsis thaliana
green fluorescent protein
nucleus
cytoplasm
glutathione redox potential
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: The adhesion and growth of vascular smooth muscle cells in cultures on carboranethiol-modified gold films
Autorzy:: Parizek, M.
Base, T.
Londesborough, M. G. S.
Lisa, V.
Bacakova, L.
Powiązania:: https://bibliotekanauki.pl/articles/285409.pdf
Data publikacji:: 2008
Wydawca:: Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:: metal coating
gold film
carboranethiol
cell adhesion
cell spreading
cell proliferation
biomaterials
tissue engineering
surgical implants
Opis:: Metal surfaces have become important over the last decade for potential surgical implants, and within this context we present here a study of the cell growth on modified gold surfaces. Gold films, deposited on glass plates and annealed with a hydrogen flame, were modified with four different carboranethiol derivatives: 1-(HS)-1,2-C2B10H11 (A), 1,2-(HS)2-1,2-C2B10H10 (B), 9,12-(HS)2-1,2-C2B10H10 (C) and 1,12-(HS)2-1,12- C2B10H10 (D). The materials engendered from these modifications were used to investigate the adhesion and growth of rat aortic smooth muscle cells cultured on these surfaces in a DMEM medium with 10% of fetal bovine serum. One day after seeding, the highest number of initially adhered cells was found on the surface of a bare gold film. However, three days after seeding, the number of cells on carboranethiol-modified gold samples B, C and D was significantly higher than the number on a bare gold film. After seven days, the number of cells on a bare gold film and on gold films modified with derivatives A, B and D was very similar, but the surface of a gold film modified with derivative C exhibited a significantly smaller number of cells. This may be explained by the exposure of the CH vertices of the carborane cluster, which are more acidic than the BH vertices exposed toward the cells in either A or B.
Źródło:: Engineering of Biomaterials; 2008, 11, no. 81-84; 117-119
1429-7248
Pojawia się w:: Engineering of Biomaterials
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Vascular smooth muscle cells in cultures on low density polyethylene modified with plasma discharge and biofunctionalization
Autorzy:: Parizek, M.
Kasalkova, N.
Bacakova, L.
Kolarova, K.
Lisa, V.
Svorcik, V.
Powiązania:: https://bibliotekanauki.pl/articles/285087.pdf
Data publikacji:: 2009
Wydawca:: Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:: Ar plasma discharge
biomaterials
low-density polyethylene
cell adhesion
cell proliferation
grafting
tissue engineering
vascular smooth muscle cells
Opis:: Low density polyethylene (LDPE) was modified by an Ar plasma discharge and then grafted with glycine (Gly), bovine serum albumin (BSA) or polyethylene glykol (PEG). Some plasma-treated samples and samples grafted with BSA were exposed to a suspension of colloidal carbon particles (C, BSA+C). Pristine LDPE and tissue culture polystyrene dishes (PSC) were used as control samples. The materials were seeded with rat aortic smooth muscle cells and incubated in a medium DMEM with 10% of fetal bovine serum. On day 1 after seeding, the cells on LDPE modified with plasma only, Gly, BSA and BSA+C adhered in similar numbers as on PSC, while the values on non-modified and PEG-modified samples were significantly lower. On day 5, the highest cell numbers were found again on LDPE with Gly, BSA and BSA+C. On day 7, the highest number of cells was found on LDPE modified only with plasma. The latter cells also dis-played the largest cell spreading area. The increased cell colonization was probably due to the formation of oxygen-containing chemical functional groups after plasma irradiation, and also due to positive effects of grafted Gly, BSA and BSA in combination with colloidal C particles.
Źródło:: Engineering of Biomaterials; 2009, 12, no. 89-91; 25-28
1429-7248
Pojawia się w:: Engineering of Biomaterials
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Improved adhesion and growth of vascular smooth muscle cells in cultures on polyethylene modified by plasma discharge
Autorzy:: Parizek, M.
Kasalkova, N.
Bacakova, L.
Kolarova, K.
Lisa, V.
Svorcik, V.
Powiązania:: https://bibliotekanauki.pl/articles/284295.pdf
Data publikacji:: 2007
Wydawca:: Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:: biomateriały
inżynieria tkankowa
Ar plasma discharge
high density and low density polyethylene
cell adhesion
cell proliferation
vascular smooth muscle cells
biomaterials
tissue engineering
Opis:: The attractiveness of synthetic polymers for cell colonization can be affected by physical and chemical modification of the polymer surface. In this study, high density polyethylene (HDPE, m.w. 0.952g/cm3) and low density polyethylene (LDPE, m.w. 0.922g/cm3) were modified by an Ar plasma discharge using Balzers SCD 050 device (exposure time 10, 50, 150 and 400 seconds, discharge power 1.7W). The material was then seeded with rat aortic smooth muscle cells (RASMC; passages 8 to 9, 17 000 cells/cm3) and incubated in a DMEM medium with 10% of fetal calf serum. On day 1 after seeding, the number of initially adhered cells was significantly higher on all modified HDPE and LDPE samples. On day 2, this difference persisted in HDPE, whereas in LDPE only the values on the samples modified by 150 and 400 seconds were significantly higher. On the 5th and 7th day, there were no significant differences in cell number among all LDPE samples. However, on the HDPE foils, significant differences were still apparent on the samples modified for 400 seconds. The cell spreading areas measured on day 1 after seeding were significantly larger on all modified LDPE samples, and, on day 2, on the HDPE samples exposed for 150s. The increased cell colonization was probably due to the formation of oxygen-containing chemical functional groups in the polymer. These results suggest that the responsiveness of the cell to the changes in physiochemical surface properties was more pronounced in HDPE than in LDPE. On both types of polyethylene, the most appropriate exposure time for the enhancement of cell adhesion and growth seemed to be 150 and 400 seconds.
Źródło:: Engineering of Biomaterials; 2007, 10, no. 67-68; 1-4
1429-7248
Pojawia się w:: Engineering of Biomaterials
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: The adhesion and growth of vascular smooth muscle cells in cultures on carboranethiol-modified gold films
Autorzy:: Parizek, M.
Base, T.
Londesborough, M. G. S.
Lisa, V.
Bacakova, L.
Powiązania:: https://bibliotekanauki.pl/articles/286249.pdf
Data publikacji:: 2008
Wydawca:: Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:: implanty chirurgiczne
biomateriały
powłoki metalowe
inżynieria materiałowa
metal coatings
gold film
carboranethiol
cell adhesion
cell spreading
cell proliferation
biomaterials
tissue engineering
surgical implants
Opis:: Metal surfaces have become important over the last decade for potential surgical implants, and within this context we present here a study of the cell growth on modified gold surfaces. Gold films, deposited on glass plates and annealed with a hydrogen flame, were modified with four different carboranethiol derivatives: 1-(HS)-1,2-C2 B10H11(A), 1,2-(HS)2-1,2-C2B10H10(B), 9,12-(HS)2-1,2-C2B10H10(C) and 1,12-(HS)2-1,12- C2B10H10(D). The materials engendered from these modifications were used to investigate the adhesion and growth of rat aortic smooth muscle cells cultured on these surfaces in a DMEM medium with 10% of fetal bovine serum. One day after seeding, the highest number of initially adhered cells was found on the surface of a bare gold film. However, three days after seeding, the number of cells on carboranethiol-modified gold samples B, C and D was significantly higher than the number on a bare gold film. After seven days, the number of cells on a bare gold film and on gold films modified with derivatives A, B and D was very similar, but the surface of a gold film modified with derivative C exhibited a significantly smaller number of cells. This may be explained by the exposure of the CH vertices of the carborane cluster, which are more acidic than the BH vertices exposed toward the cells in either A or B.
Źródło:: Engineering of Biomaterials; 2008, 11, 75; 6-8
1429-7248
Pojawia się w:: Engineering of Biomaterials
Dostawca treści:: Biblioteka Nauki