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Wyświetlanie 1-2 z 2
Tytuł:
Expression of genes modulated by epigallocatechin-3-gallate in breast cancer cells
Autorzy:
Bogacz, A.
Wolek, M.
Juskowiak, B.
Karasiewicz, M.
Kaminski, A.
Uzar, I.
Polaszewska, A.
Kostrzewa, Z.
Czerny, B.
Powiązania:
https://bibliotekanauki.pl/articles/72469.pdf
Data publikacji:
2018
Wydawca:
Instytut Włókien Naturalnych i Roślin Zielarskich
Tematy:
gene expression
epigallocatechin-3-gallate
breast cancer
cancer cell
therapy
Opis:
Breast cancer is the most common malignant cancer among women. Both drug resistance and metastasis are major problems in the treatment of breast cancer. Therefore, adjuvant therapy may improve patients’ survival and affect their quality of life. It is suggested that epigallocatechin gallate (EGCG) which is well known for its chemopreventive activity and acts on numerous molecular targets may inhibit the growth and metastasis of some cancers. Hence, discovering the metastatic molecular mechanisms for breast cancer may be useful for therapy.The aim of the study was to determine the effect of EGGC on the mRNA expression level of genes such as ZEB1, ABCB1, MDM2, TWIST1 and PTEN in MCF-7 breast cancer cells. MCF7/DOX were cultured in the presence of 0.2 μM DOX and EGCG (20-50 μM). The mRNA expression level was determined by real-time quantitative PCR using RealTime ready Custom Panel 96 kit. Our results showed an important increase (about 2-fold for 20 μM EGCG + 0.2 μM DOX and 2.5-fold for 50 μM EGCG + 0.2 μM DOX, p<0.05) in ZEB1 expression levels. In case of ABCB1 gene lack of influence on the mRNA level was observed (p>0.05). We also observed significant decrease of ZEB1 expression in MCF7 cells with 20 μM and 50 μM EGCG (p<0.05). In addition, EGCG (20 μM) caused an increase of MDM2 and PTEN mRNA levels in almost 100% (p<0.05) and 40% (p>0.05), respectively. Lack of the influence of EGCG was noted for the TWIST1 gene expression. In case of MCF7/DOX we showed an increase of mRNA level of PTEN gene about 50% (p<0.05). These results suggest that EGCG may be potentially used in adjuvant therapy in the breast cancer treatment.
Źródło:
Herba Polonica; 2018, 64, 3
0018-0599
Pojawia się w:
Herba Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection of circulating tumour cells in the breast cancer using CytoTrack system
Autorzy:
Bogacz, A.
Wolek, M.
Górska, A.
Leporowska, E.
Procyk, D.
Kolenda, P.
Litwiniuk, M.
Uzar, I.
Gryszczyńska, A.
Łowicki, Z.
Czerny, B.
Powiązania:
https://bibliotekanauki.pl/articles/2048955.pdf
Data publikacji:
2019
Wydawca:
Instytut Włókien Naturalnych i Roślin Zielarskich
Tematy:
circulating tumour cells
breast cancer
CytoTrack CT11
liquid biopsy
wolnokrążące komórki nowotworowe
rak piersi
CytroTrack CT11
płynna biopsja
Opis:
Introduction: Plants are a rich source of healing substances. Cancer is a leading cause of death worldwide while breast cancer is the most common cancer among women. Circulating tumour cells (CTCs) are potential founder cells for metastasis. Therefore, their assessment may be used for monitoring of treatment as well as detecting cancer metastatis. Hence, it is suggested that the number of CTCs may be a valuable tumour biomarker during therapy. Objective: The purpose of this study was to detect CTCs in breast cancer and to validate the method of assessment of CTC count using CytoTrack CT11 technology. Methods: MCF-7 cells were sorted by a FACSARIA flow cytometer from blood samples derived from patients who have not been diagnosed with cancer. Identification and quantitative assessment of MCF-7 cells in blood samples were determined by flow sorting. Then, blood samples containing MCF-7 cells or without MCF-7 were scanned with the use of an automated fluorescence scanning microscope. Results: In in vitro model analysing the glass CytoDisc™ with stained MCF-7 cells, we noted the correlation between the amount of observed tumour cells and expected number of tumour cells. Moreover, coefficient of variation in case of the recovery rate of the assumed number of MCF-7 cells was 30%, 17%, 18% and 15%, respectively. Conclusion: Our study suggest that CTCs could be predictive factor in patients with metastatic cancer especially in breast cancer.
Źródło:
Herba Polonica; 2019, 65, 4; 31-36
0018-0599
Pojawia się w:
Herba Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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