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Tytuł:
Biodentine management and setting time with Vicat and Vickers evaluation; a survey-based study on clinicians experience
Autorzy:
Buła, K.
Palatyńska-Ulatowska, A.
Klimek, L.
Powiązania:
https://bibliotekanauki.pl/articles/1818516.pdf
Data publikacji:
2020
Wydawca:
Stowarzyszenie Komputerowej Nauki o Materiałach i Inżynierii Powierzchni w Gliwicach
Tematy:
materials
biomaterials
biodentine
tricalcium silicate cement
setting time
materiały
biomateriały
biodentyna
cement trójwapniowy
czas wiązania
Opis:
Purpose: of this paper was to analyse clinicians’ views on the management and handling procedures of the Biodentine tricalcium silicate cement with the following evaluation of the real setting time of the material with two independent physical tests. Design/methodology/approach: A survey study included 174 clinicians who answered the questionnaire designed to collect opinions on the Biodentine management during endodontic procedures. To verify the setting time of the cement, two independent hardness tests were performed. Macroscopic evaluation was carried out using the Vicat device. Microscopic assessment with subsequent SEM observation was conducted with the aid of the Clemex appliance. Findings: 43% of respondents using Biodentine in their practice described the setting time as long or definitively too long. One fifth of the dentists surveyed continue dental procedures without waiting. The setting time tests confirmed the existence of two phases of the Biodentine setting process, which corresponds to the general definition of cement setting. After mixing of the material, the initial setting stage lasts for 15 minutes. The next one, described by the authors as “maturation” of Biodentine lasts for 120 minutes. Research limitations/implications: The material initially sets within 15 minutes, however it is not the end of the process. In certain endodontic procedures the awareness of a longer setting time of Biodentine is essential for decision-making in root canal therapy. Practical implications: It is advisable to divide the endodontic treatment with Biodentine into two separate appointments. Originality/value: From the clinicians’ perspective the setting time and correct handling of Biodentine are crucial factors in the successful endodontic therapy. The information regarding proper material management is included in this paper
Źródło:
Archives of Materials Science and Engineering; 2020, 103, 2; 75--85
1897-2764
Pojawia się w:
Archives of Materials Science and Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Potential activation of the immune system on metallic materials for bone implants
Autorzy:
Stranavova, L.
Bacakova, M.
Novotna, K.
Bacakova, L.
Fencl, J.
Powiązania:
https://bibliotekanauki.pl/articles/285314.pdf
Data publikacji:
2012
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
bone implants
metallic materials
biomaterials
Opis:
Titanium and stainless steel are metallic materials that have been in use for a long time in orthopedics, traumatology and stomatology. These metals are strong, corrosion-resistant and biocompatible. However, metallic materials have some disadvantages in comparison with the natural bone, particularly their relatively high specific weight and toughness. For example, the Young's modulus of AISI316L stainless steel, Co-Cr alloys and Ti-6Al-4V alloy, i.e. materials frequently used for implantation into bone, ranges between 110-220 GPa, while the Young's modulus of bone tissue is 10-40 GPa [1]. In addition, these metals can release cytotoxic, allergenic and immunogenic ions, which can affect their biocompatibility [2, 3]. Implantation is a special type of transplantation process, in which the implant is inserted into the body, usually in order to replace an irreversibly damaged tissue. However, the immune system recognizes the implant as a foreign substance and attacks it with its effector mechanisms. Just as it can reject other types of transplants, the immune system can reject an artificial implant. To prevent rejection of an implant, it is important to study the potential activation of the immune system. This study has investigated the biocompatibility of samples made of pure titanium (according to quality standard ISO 5832-2) and corrosion-resistant steel (quality standards ISO 5832-1 and AISI 316L), obtained from Beznoska Ltd. (Kladno, Czech Republic), and the potential activation of the immune system by these materials. In addition to Fe, the steel samples contained C (max. 0.025 wt.%), Si (0.6 wt.%), Mn (1.7 wt.%), P (max. 0.025wt.%), S (max. 0.003 wt.%), Cr (17.5 wt.%), Ni (13.5 wt.%), Mo (2.8 wt.%), and Cu (max. 0.1 wt. %). The materials were used in the form of square samples (9x9 mm or 30x30 mm, thick¬ness 1 mm). Both the Ti samples and the steel samples were ground with SiO2. The surface of the steel samples was then treated by polishing with Al2O3 paste (grain size up to 1 um), while the surface of the Ti samples, i.e. a material not suitable for polishing, was finished by brushing using another type of Al2O3 paste with slightly larger grains. Thus, the surface of the steel samples was finally smoother and glossy, while the Ti surface was rougher and matte. For the in vitro biocompatibility tests, human osteoblast-like MG 63 cells (European Collection of Cell Cultures, Salisbury, UK) were used. The smaller samples (9x9 mm) were inserted into polystyrene 24-well cell culture plates (TPP, Trasadingen, Switzerland; well diameter 1.5 cm). Each well contained 25 000 cells (approx. 14 150 cells/cm2) and 1.5 ml of Dulbecco's Modified Eagle Minimum Essential Medium (DMEM; Sigma, USA, Cat. No. 10270-106) supplemented with 10% foetal bovine serum (FBS; Gibco, Cat. No. 10270-106) and gentamicin (40 /jg/ml, LEK, Slovenia). These samples were used for evaluating the size of the cell spreading area (day 1), and for evaluating cell shape and cell viability (days 1, 4 and 7 after seeding). The size of the cell spreading area was measured using Atlas Software (Tescan Ltd., Brno, Czech Republic). The viability of the cells was determined by the LIVE/ DEAD viability/cytotoxicity kit for mammalian cells (Invitrogen, Molecular Probes, USA). The larger samples (30x30 mm) were inserted into GAMA polystyrene dishes (diameter 5 cm; GAMA Group Joint-Stock Company, Ceske Budejovice, Czech Republic) and seeded with 300 000 cells/dish (approx. 15 300 cells/cm2) suspended in 9 ml of the above mentioned culture medium. These samples were used for evaluating the cell number on days 1, 4 and 7 after seeding, using a Beckman Vi-CELL XR Cell Analyser automatic cell counter. For the in vitro analysis of markers of osteogenic differentiation and cell immune activation, human osteoblast-like MG 63 cells (European Collection of Cell Cultures, Salisbury, UK) were used. The samples (9x9 mm) were inserted into polystyrene 24-well cell culture plates (TPP, Trasadingen, Switzerland; well diameter 1.5 cm). Each well contained 25 000 cells (approx. 14 150 cells/cm2) and 1.5 ml of Dulbecco's Modified Eagle Minimum Essential Medium (DMEM; Sigma, USA, Cat. No. 10270-106) supplemented with 10% foetal bovine serum (FBS; Gibco, Cat. No. 10270-106) and gentamicin (40 jg/ml, LEK, Slovenia). The cells were cultured for 1, 4, or 7 days at 37°C in a humidified atmosphere of 5% of CO2 in the air. On day 4 after seeding, the medium was changed; one half of the samples contained standard medium DMEM with 10% foetal bovine serum and gentamicin (40 jg/ml) mentioned above, and the second half contained osteogenic medium, i.e. the standard medium further supplemented with ß-glycerophosphate, L-glutamin, ascorbic acid, dihydroxyvitamin D3, dexamethason, 10% foetal bovine serum and gentamicin (40 jg/ml). Using an Enzyme-Linked ImmunoSorbent Assay (ELISA), we measured the concentration of the Inter¬cellular Adhesion Molecule-1 (ICAM-1, a marker of cell immune activation) and osteocalcin (a marker of osteogenic cell differentiation). These measurements were performed in homogenates of cells on days 4 and 7 after seeding, and the concentration of both markers was measured per cell or per mg of protein. On day 7, the amount of osteocalcin was measured and compared in cells cultured in the standard and osteogenic media. We also measured TNF-а and IL- 1ß, i.e. other markers of cell immune activation. These cytokines are important mediators of the inflammatory response, and they are involved in a variety of cellular activities, including cell proliferation and differentiation. We measured the secretion of these markers into the cell culture medium in murine macrophage-like RAW264.7 cells (American Type Culture Collection, Manassas, VA). The samples (9x9 mm) were inserted into polystyrene 24-well cell culture plates (TPP, Tra- sadingen, Switzerland; well diameter 1.5 cm). Each well contained 30,000 (approx. 16 980 cells/cm2) cells and 1.5 ml of the culture medium. RAW 264.7 cells were cultured in the RPMI-1640 medium (Sigma; 10% fetal bovine serum, 40 jg/mL gentamicin). After 7 days of cultivation, the cell culture medium was collected and used for measuring the concentration of TNF-а and IL-1ß by a sandwich ELISA using commercially available kits. A mouse TNF-а kit and an IL- 1ß Quantikine ELISA kit were used for the RAW 264.7 cells. Both kits were purchased from R and D Systems (Minneapolis, MN) and used according to the manufacturer's protocol. The results indicated that the number of initially adhering MG 63 cells on day 1 after seeding was significantly lower on the titanium (5320±390 cells/cm2) and on the stainless steel (4110±370 cells/cm2) than on the control polystyrene culture dishes (7740±350 cells/cm2). However, on day 4 after seeding, the cell population density on both metallic materials became significantly higher than on the control polystyrene dishes (75200±2890 cells/cm2 on Ti and 90 870±2350 cells/cm2 on steel vs. 56440±1180 cells/cm2 on polystyrene). This suggests faster cell proliferation on both metallic materials than on polystyrene. At the same time, the cell number on the stainless steel samples was significantly higher than on the Ti samples. On day 7, the differences in the number of adhered cells on the two metals and on the control polystyrene substrate was on an average similar (from 328780±680 cells/cm2 to 362200±760 cells/cm2). The cell viability on all tested materials was almost 100% in all culture intervals. The morphology of the cells adhered on the studied materials was similar to the morphology of the cells on the control polystyrene dishes, i.e. the cells were mostly flat and polygonal, and the size of their cell spreading areas was similar on all tested materials. The cells were distributed homogeneously on the entire material surface, and on day 4 they started to form confluent cell layers. On day 4, we measured the amount of ICAM-1 by the ELISA test. This immunoglobulin molecule is typically expressed on cells of the immune system, but it is also expressed on other cell types, including MG 63, during their immune activation, e.g. by an artificial growth support. In this case, ICAM-1 molecules on cells are bound byß2-integrin receptors on inflammatory cells (for a review, see [4]). Surprisingly, titanium seemed to be more immunogenic than stainless steel, which was indicated by a higher concentration of ICAM-1 per cell and mg of protein in cells on day 4 after seeding. However, on day 7, there was no difference between the concentrations of ICAM-1 per cell and mg of protein in cells on titanium and on stainless steel. The second molecule that we measured was osteocalcin, a calcium-binding extracellular matrix glycoprotein, an important marker of the bone formation process. The concentration of osteocalcin on day 4 in the standard culture medium was higher in MG 63 cells on the titanium and stainless steel than on the control polystyrene samples. This could be explained by the fact that the metals are harder than polystyrene. It is known that harder substrates promote osteogenic cell differentiation, while softer substrates direct the cell differentiation towards neural or muscle phenotype [5]. In addition, the osteogenic differentiation was further supported by the osteogenic medium, as indicated by a higher concentration of osteocalcin in cells grown in this medium compared to cells in the standard medium on day 7 after seeding. On day 7 after seeding murine macrophage-like RAW264.7 cells on the tested materials, the concentration of TNF-а in the culture medium ranged on an average from 57.10 to 79.39 pg per 2000000 cells. The concentration of TNF-а in the medium from Ti and Fe was significantly higher than in the medium from the control polystyrene dishes. The highest value (79.39 pg/2000000 cells) was found in the medium taken from RAW264.7 cells on Ti. The second molecule that we tested was IL-1ß. No significant differences in the concentration of IL-1ß were detected in the culture medium obtained from RAW264.7 cells on all tested materials. In other words, neither type of metallic material, i.e. Ti and Fe, evoked significantly higher production of IL-1ß by RAW 264.7 cells than standard polystyrene cell culture dishes. It can be concluded that the tests of biocompatibility and immune activation confirmed that titanium and stainless are promising for construction of bone implants and for good integration with the surrounding bone tissue.
Źródło:
Engineering of Biomaterials; 2012, 15, no. 116-117 spec. iss.; 130-131
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Selenium containing hydroxyapatite granules as drug carriers for risedronate
Autorzy:
Kolmas, J.
Pajor, K.
Pajchel, Ł.
Olędzka, E.
Sobczak, M.
Powiązania:
https://bibliotekanauki.pl/articles/285367.pdf
Data publikacji:
2016
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
hydroxyapatite
drugs
biomaterials
Źródło:
Engineering of Biomaterials; 2016, 19, 138; 70
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of cellulose-based biomaterials in vascular tissue engineering - a review and our experience
Autorzy:
Bacakova, L.
Novotna, K.
Parizek, M.
Havelka, P.
Sopuch, T.
Powiązania:
https://bibliotekanauki.pl/articles/284148.pdf
Data publikacji:
2012
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
biomaterials
tissue engineering
vascular
Opis:
Artificial vascular replacements used in current clinical practice are fabricated from polyethylene terephthalate (PET, e.g. Dacron) orpolyterafluoroethylene (PTFE, e.g. Teflon). Older materials used earlier for constructing vascular prostheses are polyamide (Nylon), polyvinyl alcohol (Ivalon) and polyacrylonitrile (Orlon). New promising materials include polyurethane and a wide range of biodegradable synthetic or nature-derived polymers, which are usually designed as temporary scaffolds for vascular cells forming a new regenerated blood vessel wall (for a review, see [1]). One of the nature-derived polymers is cellulose and its derivatives and composites with other materials. Cellulose is the most abundant biopolymer on Earth. It is a polysaccharide consisting of a linear chain of several hundred to over ten thousand ß(1\to 4) linked D-glucose units [2,3]. Cellulose is the structural component of the primary cell wall of green plants, many forms of algae and the oomycetes. In plant cells, cellulose microfibrils are synthesized at the plasma membrane by hexameric protein complexes, also known as cellulose synthase complexes [4]. Some species of bacteria secrete cellulose to form biofilms. For industrial use, cellulose is mainly obtained from wood pulp and cotton. For tissue engineering applications, bacterial cellulose has been predominantly used, mainly that synthesized by Acetobacterxylinum. Bacterial cellulose is identical to plant cellulose in chemical structure, but it can be produced without contaminant molecules, such as lignin and hemicelluloses, and does not require intensive purification processes. In addition, it is remarkable for its mechanical strength, its ability to be engineered structurally and chemically at nano-, micro-, and macroscales, its biocompatibility and chemical and morphologic controllability [5]. Bacterial cellulose has been used for experimental engineering of bone tissue [6], cartilage [7], skin [8], heart valve [9], and also for urinary reconstruction and diversion [10]. One of the first attempts at vascular tissue engineering was made with cellulose fibers, which were used for constructing three-dimensional vascularized tissue in vitro. These fibers were immobilized with fibronectin in order to improve cell adhesion, and were seeded with bovine coronary artery smooth muscle cells. These cells proliferated on the scaffolds and, after they formed multilayers on the fibers, the fibers were removed by enzymatic digestion using cellulase. The remaining smooth muscle cell aggregates maintained lumens after this procedure, and thus mimicked newly-formed blood vessels [11]. Similarly, three-dimensional nanofibrous scaffolds with micropores made of bacterial cellulose allowed attachment and proliferation of human saphenous vein smooth muscle cells on the surface and also in the inside of the scaffolds [12]. In addition, the mechanical properties of nanofibrous bacterial cellulose scaffolds, evaluated by the shape of the stress-strain response, were reminiscent of the properties of the carotid artery, most probably due to the similarity in architecture of the nanofibril network [13]. The adhesion and growth of vascular endothelial cells was also supported by cellulose-based scaffolds, namely by nanofibrous bacterial cellulose or cellulose acetate scaffolds, especially if these scaffolds were functionalized with RGD-containing oligopeptides, i.e. ligands for integrin adhesion receptors on cells [14, 15], or if they were combined with chitosan [16]. The angiogenic response to bacterial cellulose was also observed under in vivo conditions, i.e. after implantation of these scaffolds in the form of dorsal skinfold chambers into Syrian golden hamsters [17]. Cellulose has also been used for creating tubular structures designed for replacing small-caliber vessels. Hollow-shaped segments of bacterial cellulose were created with a length of 10 mm, an inner diameter of 3.0-3.7 mm and a wall thickness of 0.6 -1.0 mm. These grafts were used to replace the carotid arteries of eight pigs. After a follow-up period of 3 months, seven grafts (87.5%) remained patent, whereas one graft was found to be occluded. All patent grafts developed a single inner layer of endothelium with a basement membrane and a thin layer of collagen, followed by a concentric medial layer containing smooth muscle cells and cellulose, and an outer layer of fibrous cells [18]. Similarly, bacterial cellulose grafts 4 cm in length and 4 mm in internal diameter were implanted bilaterally in the carotid arteries of eight sheep. Although 50% of the grafts occluded within 2 weeks, all patent grafts developed a confluent inner layer of endothelial- like cells [19]. In addition, the mechanical properties of tubular structures created from bacterial cellulose seemed to be advantageous for vascular tissue engineering. For example, these structures exhibited a compliance response similar to that of human saphenous vein [20]. In our experiments, we have concentrated on cellulose-based materials modified with oxidation and/or functionalization with biomolecules. We have prepared fibrous scaffolds made of non-oxidized viscose, dialdehyde cellulose and 6-carboxycellulose with 2.1 wt.% or 6.6 wt.% of -COOH groups. In addition, all these material types were functionalized with arginine, i.e. an amino acid with a basic side chain, or with chitosan, in order to balance (compensate) the relatively acid character of oxidized cellulose molecules. Two groups of samples with and without functionalization were then seeded with vascular smooth muscle cells (VSMC) derived from the rat thoracic aorta by an explantation method [21]. We found that the oxidized cellulose with 2.1 wt.% of-COOH groups was the most appropriate of all the tested materials for colonization with VSMC. The cells on this material achieved an elongated shape, while they were spherical in shape on the other materials. In addition, the numbers of cells obtained in one week after seeding and the concentration of alpha-actin and SM1 and SM2 myosins, measured per mg of protein, were significantly higher on oxidized cellulose with 2.1 wt.% of -COOH groups. Functionalization with arginine and chitosan improved the cell adhesion, but usually only slightly. The most apparent increase in cell number after functionalization was observed on oxidized cellulose with 2.1 wt.% of -COOH groups functionalized with chitosan, and on viscose functionalized with chitosan or arginin. However, the cells on all samples proliferated slowly and with a non-significant increase in cell population densities from day 1 to 7 after seeding. This suggests that cellulose-based materials can be used in applications where high proliferation activity of vascular smooth muscle cells is not desirable. They can therefore be used on vascular prostheses, where excessive VSMC proliferation can lead to the restenosis of the graft. Alternatively, cell proliferation might be enhanced by some other more efficient modification. This would require further research.
Źródło:
Engineering of Biomaterials; 2012, 15, no. 116-117 spec. iss.; 128-130
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zmiana odporności korozyjnej materiałów po obróbce powierzchniowej do zastosowań biomedycznych
Corrosion resistance changes of materials for biomedical applications after surface treatment
Autorzy:
Mendzik, K.
Lubas, M.
Jasiński, J.
Jeziorski, L.
Szota, M.
Powiązania:
https://bibliotekanauki.pl/articles/284884.pdf
Data publikacji:
2007
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
biomateriały
implanty
biomaterials
implants
Źródło:
Engineering of Biomaterials; 2007, 10, no. 67-68; 42-44
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Osteoblast behaviour on novel whey protein isolate hydrogels
Autorzy:
Stählke, S.
Mazur, K.
Krężel, A.
Żydek, J.
Pietryga, K.
Pamuła, E.
Keppler, J. K.
Nebe, J. B.
Douglas, T. E. L.
Powiązania:
https://bibliotekanauki.pl/articles/285936.pdf
Data publikacji:
2018
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
hydrogels
biomaterials
osteogenic cells
Źródło:
Engineering of Biomaterials; 2018, 21, 148; 105
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Improved adhesion and growth of vascular smooth muscle cells in cultures on polyethylene modified by plasma discharge
Autorzy:
Parizek, M.
Kasalkova, N.
Bacakova, L.
Kolarova, K.
Lisa, V.
Svorcik, V.
Powiązania:
https://bibliotekanauki.pl/articles/284295.pdf
Data publikacji:
2007
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
biomateriały
inżynieria tkankowa
Ar plasma discharge
high density and low density polyethylene
cell adhesion
cell proliferation
vascular smooth muscle cells
biomaterials
tissue engineering
Opis:
The attractiveness of synthetic polymers for cell colonization can be affected by physical and chemical modification of the polymer surface. In this study, high density polyethylene (HDPE, m.w. 0.952g/cm3) and low density polyethylene (LDPE, m.w. 0.922g/cm3) were modified by an Ar plasma discharge using Balzers SCD 050 device (exposure time 10, 50, 150 and 400 seconds, discharge power 1.7W). The material was then seeded with rat aortic smooth muscle cells (RASMC; passages 8 to 9, 17 000 cells/cm3) and incubated in a DMEM medium with 10% of fetal calf serum. On day 1 after seeding, the number of initially adhered cells was significantly higher on all modified HDPE and LDPE samples. On day 2, this difference persisted in HDPE, whereas in LDPE only the values on the samples modified by 150 and 400 seconds were significantly higher. On the 5th and 7th day, there were no significant differences in cell number among all LDPE samples. However, on the HDPE foils, significant differences were still apparent on the samples modified for 400 seconds. The cell spreading areas measured on day 1 after seeding were significantly larger on all modified LDPE samples, and, on day 2, on the HDPE samples exposed for 150s. The increased cell colonization was probably due to the formation of oxygen-containing chemical functional groups in the polymer. These results suggest that the responsiveness of the cell to the changes in physiochemical surface properties was more pronounced in HDPE than in LDPE. On both types of polyethylene, the most appropriate exposure time for the enhancement of cell adhesion and growth seemed to be 150 and 400 seconds.
Źródło:
Engineering of Biomaterials; 2007, 10, no. 67-68; 1-4
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Investigation on the influence of ZrC coatings structure on their resistance to corrosion and antimicrobial properties
Autorzy:
Ratajski, J.
Szparaga, Ł.
Mydłowska, K.
Dobruchowska, E.
Czerwińska, E.
Gilewicz, A.
Powiązania:
https://bibliotekanauki.pl/articles/284384.pdf
Data publikacji:
2018
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
coatings
corrosion
biomaterials
Źródło:
Engineering of Biomaterials; 2018, 21, 148; 72
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of pre-coarsening on architectural and mechanical properties of highly porous titanium dioxide scaffolds for bone tissue engineering
Autorzy:
Reczyńska, K.
Rumian, Ł.
Pamuła, E.
Haugen, H. J.
Tiainen, H.
Powiązania:
https://bibliotekanauki.pl/articles/284566.pdf
Data publikacji:
2014
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
bone tissue engineering
porous titanium
biomaterials
Źródło:
Engineering of Biomaterials; 2014, 17, no. 128-129; 96-98
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Enrichment of thermosensitive chitosan hydrogels with glycerol and alkaline phosphatase for bone tissue engineering applications
Autorzy:
Douglas, T. E. L.
Krok-Borkowicz, M.
Macuda, A.
Pietryga, K.
Pamuła, E.
Powiązania:
https://bibliotekanauki.pl/articles/306906.pdf
Data publikacji:
2016
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
biomateriały
kompozyty
mineralizacja
hydrożel
biomaterials
composites
mineralization
hydrogels
Opis:
Thermosensitive injectable chitosan hydrogels can be formed by neutralization of acidic chitosan solutions with sodium betaglycerophosphate (Na-β-GP) coupled with increasing temperature to body temperature. Such hydrogels have been considered for applications in bone regeneration. In this study, chitosan hydrogels were enriched with glycerol and the enzyme alkaline phosphatase (ALP) with a view to improving their suitability as materials for bone tissue engineering. Mineral formation was confirmed by infrared spectroscopy (FTIR) and increases in the mass fraction of the hydrogel not consisting of water. Incorporation of ALP in hydrogels followed by incubation in a solution containing calcium ions and glycerophosphate, a substrate for ALP, led to formation of calcium phosphate within the hydrogel. MG-63 osteoblast-like cells were cultivated in eluates from hydrogels containing ALP and without ALP at different dilutions and directly on the hydrogel samples. Hydrogels containing ALP exhibited superior cytocompatibility to ALP-free hydrogels. These results pave the way for the use of glycerol- and ALP-enriched hydrogels in bone regeneration.
Źródło:
Acta of Bioengineering and Biomechanics; 2016, 18, 2; 51-57
1509-409X
2450-6303
Pojawia się w:
Acta of Bioengineering and Biomechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Vascular smooth muscle cells in cultures on low density polyethylene modified with plasma discharge and biofunctionalization
Autorzy:
Parizek, M.
Kasalkova, N.
Bacakova, L.
Kolarova, K.
Lisa, V.
Svorcik, V.
Powiązania:
https://bibliotekanauki.pl/articles/285087.pdf
Data publikacji:
2009
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
Ar plasma discharge
biomaterials
low-density polyethylene
cell adhesion
cell proliferation
grafting
tissue engineering
vascular smooth muscle cells
Opis:
Low density polyethylene (LDPE) was modified by an Ar plasma discharge and then grafted with glycine (Gly), bovine serum albumin (BSA) or polyethylene glykol (PEG). Some plasma-treated samples and samples grafted with BSA were exposed to a suspension of colloidal carbon particles (C, BSA+C). Pristine LDPE and tissue culture polystyrene dishes (PSC) were used as control samples. The materials were seeded with rat aortic smooth muscle cells and incubated in a medium DMEM with 10% of fetal bovine serum. On day 1 after seeding, the cells on LDPE modified with plasma only, Gly, BSA and BSA+C adhered in similar numbers as on PSC, while the values on non-modified and PEG-modified samples were significantly lower. On day 5, the highest cell numbers were found again on LDPE with Gly, BSA and BSA+C. On day 7, the highest number of cells was found on LDPE modified only with plasma. The latter cells also dis-played the largest cell spreading area. The increased cell colonization was probably due to the formation of oxygen-containing chemical functional groups after plasma irradiation, and also due to positive effects of grafted Gly, BSA and BSA in combination with colloidal C particles.
Źródło:
Engineering of Biomaterials; 2009, 12, no. 89-91; 25-28
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Elektroforetyczne osadzanie hydroksyapatytu na powierzchni stopów NiTi wykazujacych pamięć kształtu
Electrophoretic deposition of hydroxyapatite coatings on NiTi shape memory alloy
Autorzy:
Goryczka, T.
Szaraniec, B.
Dudek, K.
Zych, Ł.
Freitag, M.
Lelątko, J.
Powiązania:
https://bibliotekanauki.pl/articles/285852.pdf
Data publikacji:
2011
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
hydroksyapatyt
pamięć kształtu
biomateriały
hydroxyapatite
shape memory
biomaterials
Źródło:
Engineering of Biomaterials; 2011, 14, no. 106-108; 124-128
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Investigation of mechanical and anticorrosive properties of ZrC coatings deposited by magnetron sputtering technique
Autorzy:
Szparaga, Ł.
Mydłowska, K.
Dobruchowska, E.
Bartosik, P.
Gilewicz, A.
Ratajski, J.
Powiązania:
https://bibliotekanauki.pl/articles/286251.pdf
Data publikacji:
2018
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
coatings
biomaterials
magnetron sputtering technique
Źródło:
Engineering of Biomaterials; 2018, 21, 148; 73
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Impact of human serum on HAp-glucan composite
Autorzy:
Borkowski, L.
Lübek, T.
Jojczuk, M.
Nogalski, A.
Belcarz, A.
Pałka, K.
Hajnos, M.
Ginalska, G.
Powiązania:
https://bibliotekanauki.pl/articles/285218.pdf
Data publikacji:
2018
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
biomaterials
bone tissue
regeneration
Źródło:
Engineering of Biomaterials; 2018, 21, 148; 10
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comprehensive biological evaluation of biomaterials used in spinal surgery
Autorzy:
Komorowski, P.
Kamińska, M.
Jakubowski, W.
Szymański, W.
Walczyńska, M.
Siatkowska, M.
Działoszyńska, K.
Sokołowska, P.
Białkowska, K.
Wasiak, T.
Elgalal, M.
Makowski, K.
Kierzkowska, A.
Ciupik, L.
Walkowiak, B.
Powiązania:
https://bibliotekanauki.pl/articles/283763.pdf
Data publikacji:
2017
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
biomaterials
spinal surgery
biological tests
Źródło:
Engineering of Biomaterials; 2017, 20, no. 143 spec. iss.; 23
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł

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