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Wyszukujesz frazę "Różalski, Antoni" wg kryterium: Autor

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    Wyświetlanie 1-3 z 3
    Tytuł:
    Various intensity of Proteus mirabilis-induced crystallization resulting from the changes in the mineral composition of urine
    Autorzy:
    Torzewska, Agnieszka
    Różalski, Antoni
    Powiązania:
    https://bibliotekanauki.pl/articles/1039148.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Proteus mirabilis
    crystallization
    urinary stones
    mineral composition
    Opis:
    Infectious urolithiasis is a result of recurrent and chronic urinary tract infections caused by urease-positive bacteria, especially Proteus mirabilis. The main role in the development of this kind of stones is played by bacterial factors such as urease and extracellular polysaccharides, but urinary tract environment also contributes to this process. We used an in vitro model to establish how the changes in the basic minerals concentrations affect the intensity of crystallization which occurs in urine. In each experiment crystallization was induced by an addition of P. mirabilis to artificial urine with a precisely defined chemical composition. Crystallization intensity was determined using the spectrophotometric microdilution method and the chemical composition of formed crystals was established by atomic absorption spectroscopy and colorimetric methods. Increasing the concentration of all crystals forming ions such as Mg2+, Ca2+ and phosphate strongly intensified the process of crystallization, whereas reducing the amount of these components below the proper physiological concentration did not affect its intensity. The inhibitory influence of citrate on calcium and magnesium phosphate crystallization and competitive actions of calcium and oxalate ions on struvite crystals formation were not confirmed. In the case of infectious stones the chemical composition of urine plays an important role, which creates a necessity to support the treatment by developing a model of proper diet.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 1; 127-132
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of nutrient and stress factors on polysaccharides synthesis in Proteus mirabilis biofilm
    Autorzy:
    Moryl, Magdalena
    Kaleta, Aleksandra
    Strzelecki, Kacper
    Różalska, Sylwia
    Różalski, Antoni
    Powiązania:
    https://bibliotekanauki.pl/articles/1039353.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Proteus mirabilis
    exopolysaccharides
    biofilm
    nutrient
    stress factors
    Opis:
    The extracellular matrix in biofilm consists of water, proteins, polysaccharides, nucleic acids and phospholipids. Synthesis of these components is influenced by many factors, e.g. environment conditions or carbon source. The aim of the study was to analyse polysaccharides levels in Proteus mirabilis biofilms after exposure to stress and nutritional conditions. Biofilms of 22 P. mirabilis strains were cultivated for 24, 48, 72 hours, 1 and 2 weeks in tryptone soya broth or in modified media containing an additional amount of nutrients (glucose, albumin) or stress factors (cefotaxime, pH 4, nutrient depletion). Proteins and total polysaccharides levels were studied by Lowry and the phenol-sulphuric acid methods, respectively. Glycoproteins levels were calculated by ELLA with the use of selected lectins (WGA and HPA). For CLSM analysis dual fluorescent staining was applied with SYTO 13 and WGA-TRITC. In optimal conditions the levels of polysaccharides were from 0 to 442 μg/mg of protein and differed depending on the strains and cultivation time. The agents used in this study had a significant impact on the polysaccharides synthesis in the P. mirabilis biofilm. Among all studied components (depending on tested methods), glucose and cefotaxime stimulated the greatest production of polysaccharides by P. mirabilis strains (more than a twofold increase). For most tested strains the highest amounts of sugars were detected after one week of incubation. CLSM analysis confirmed the overproduction of N-acetyloglucosamine in biofilms after cultivation in nutrient and stress conditions, with the level 111-1134%, which varied depending on the P. mirabilis strain and the test factor.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 1; 133-139
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antimicrobial, antiadhesive and antibiofilm potential of lipopeptides synthesised by Bacillus subtilis, on uropathogenic bacteria
    Autorzy:
    Moryl, Magdalena
    Spętana, Magdalena
    Dziubek, Klaudia
    Paraszkiewicz, Katarzyna
    Różalska, Sylwia
    Płaza, Grażyna
    Różalski, Antoni
    Powiązania:
    https://bibliotekanauki.pl/articles/1038900.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    uropathogens
    biofilm
    lipopeptides
    Opis:
    The aim of this study was to investigate the antimicrobial effect of lipopeptide biosurfactants from surfactin, iturin and fengycin families, synthesised by the Bacillus subtilis I'1a strain, on uropathogenic bacteria, including the effects on planktonic growth, processes of biofilm formation and dislodging. Antimicrobial activity was tested against 32 uropathogenic strains belonging to 12 different species of Gram-negative and Gram-positive bacteria. The sensitivity of 25 tested bacterial strains to the B. subtilis I'1a filtrate was confirmed by an agar diffusion assay. None of the strains seemed to be sensitive to pure surfactin at concentrations ranging from 0.1 mg × ml-1 to 0.4 mg ml-1. After the treatment of uropathogens with B. subtilis lipopeptides, the metabolic activity of planktonic cells was inhibited by 88.05±3.96% in the case of 21 studied uropathogens, the process of biofilm formation was reduced by 88.15±4.77% in the case of 24 uropathogens and mature biofilms of 18 strains were dislodged by about 81.20±4.72%. Ten strains of uropathogenic bacteria were selected to study the antimicrobial activity of surfactin (concentrations 0.1, 0.2 and 0.4 mg × ml-1). Surfactin had no influence on the metabolic activity of planktonic forms of uropathogens, however, biofilms of 5 tested strains were reduced by 64.77±9.05% in the presence of this biosurfactant at the concentration 0.1 mg × ml-1. The negative effect of the compound on the biofilm formation process was observed at all concentrations used. The above-described results were fully confirmed by CLSM. It could suggest that synergistic application of biosurfactants could be efficient in uropathogen eradication.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 4; 725-732
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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