Temat: anther culture - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Androgenic potential of polish barley cultivars in anther culture in vitro
Autorzy:: Kruczkowska, H.
Pawłowska, H.
Skucińska, B.
Powiązania:: https://bibliotekanauki.pl/articles/2198872.pdf
Data publikacji:: 2005-06-23
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: androgenesis
anther culture
barley
cold or mannitol pretreatment
Opis:: The aim of the research was to assess the androgenic potential of 21 Polish cultivars of spring and winter barley. On average, a few green plants developed per 100 anthers. Cold pretreatment proved conducive to the formation of embryos/calli, while mannitol pretreatment favoured green plant development. The first embryos/calli formed on the induction medium developed most efficiently. The authors chose FHG medium supplemented with 1 mg/l BAP and 0.2 mg/l 2,4-D (induction of androgenesis) and FHG medium supplemented with 0.4 mg/l BAP and 3% maltose (plant development).
Źródło:: Plant Breeding and Seed Science; 2005, 51; 57-66
1429-3862
2083-599X
Pojawia się w:: Plant Breeding and Seed Science
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Anther and microspore cultures of barley and wheat
Autorzy:: Kasha, K J
Ziauddin, A
Cho, U H
Simion, E
Petroski, R
Cistue, L
Powiązania:: https://bibliotekanauki.pl/articles/2046611.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: transformation
isolated microspore
doubled haploid
wheat
microspore culture
barley
anther culture
Opis:: This paper briefly cites the various procedures for the production of doubled haploids in barley and wheat. Various associated terms are defined and the factor involved in haploid production are outlined. Isolated microspore cultures offer some advantages over anther culture. Our current procedures for isolated microspore culture of barley and wheat are presented and compared.
Źródło:: Journal of Applied Genetics; 1997, 38, 4; 373-380
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Gene segregation in a barley DH population
Autorzy:: Szarejko, I
Maluszynski, M
Polok, K
Zbieszczyk, J
Powiązania:: https://bibliotekanauki.pl/articles/2046613.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: doubled haploid
barley
haploid plant
gametoclonal variation
gametic selection
anther culture
population
gene segregation
Opis:: A large population of anther culture-derived barley regenerants and their progeny was tested for allele segregation at 1 isozyme and 8 morphological marker loci. The segregation of genetic markers was examined separately for haploid, diploid and polyploid regenerants. All the 9 analysed genes except al (albino lemma) on chromosome 3 segregated according to the expected 1:1 ratio in the microspore-derived barley population. There was no difference in allele distribution between haploid and diploid regenerants. Among the limited number of 34 analysed tetraploids a significant excess of the dominant allele at locus о (orange lemma) of chromosome 6 was also observed. The recombination frequency between linked genes (n - lk2 on chromosome 1 and r - s on chromosome 7) estimated in the DH population did not differ significantly from recombination rates calculated in F₂ progeny or presented in barley chromosome maps. The phenomenon of gametic selection is discussed in relation to the genotype dependency of anther culture response and procedures used for DH production in barley.
Źródło:: Journal of Applied Genetics; 1997, 38, 4; 415-424
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Application of in vitro culture techniques to barley [Hordeum vulgare L.] improvement
Autorzy:: Ullrich, S E
Kleinhofs, A.
Hou, L.
Jones, B.L.
Powiązania:: https://bibliotekanauki.pl/articles/2044453.pdf
Data publikacji:: 1998
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: tissue culture
doubled haploid
mutagenesis
breeding programme
anther culture
in vitro
callus culture
somaclonal variation
barley
mutation
Hordeum vulgare
embryo
molecular biology
Opis:: Several aspects of in vitro culture have potential for cereal improvement. This paper focuses on evaluation of somaclonal variation (SV) from immature embryo callus culture, and doubled haploid (DH) production via anther culture in barley. Genetically stable SV was observed for several seedling morphological traits such as albino, yellow, light green and lethal. SV occurred at approximately half the frequency of azide-induced mutagenesis. The potential for widespread application of anther culture-mediated DH production in barley breeding and genetic studies was increased through culture procedure improvements and understanding the inheritance of anther culture response. Methodology improvements included substitution of inexpensive gelrite for expensive ficoll or agarose, ability to grow anther donor plants under field as well as growth chamber conditions and flexibility in cold pretreatment/storage of anther donor spikes for 4-6 weeks prior to anther plating. From diallel analysis, inheritance of anther culture response was complex with additive and dominance effects for embryoid formation, total plant regeneration and green plant regeneration and reciprocal effects (maternal) for green plant regeneration. High x low responder crosses generated F₁’s that were intermediate in response and low x low crosses sometimes produced F₁ heterosis for green plant regeneration. Therefore, some recalcitrant types appear to be usable in anther culture DH production systems within a breeding program.
Źródło:: Journal of Applied Genetics; 1998, 39, 1; 49-58
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Cytological and genetic evaluation of anther culture-derived doubled haploids in barley
Autorzy:: Szarejko, I
Falk, D E
Janusz, A
Nabialkowska, D
Powiązania:: https://bibliotekanauki.pl/articles/2046612.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: cytological analysis
doubled haploid
morphological variation
genotype
barley
genetic evaluation
gametoclonal variation
chlorophyll mutation test
Hordeum vulgare
anther culture
Opis:: Gametoclonal variation among anther culture-derived plants of three barley genotypes was estimated on the basis of cytological analysis (DH1, DH2 generation), observation of morphological variants (DH2, DH3) and chlorophyll mutation test (DH2, DH3). Individual head rows were grown in the field to detect possible chimeric structure of regenerants and to assess the number of variants and mutations in each line. Spontaneously doubled plants were the most frequent class (70%) among regenerants and almost 90% of them were completely fertile. There was a difference in proportion of haploids produced by different genotypes, but the highest frequency observed did not exceed 21%. The remaining regenerants were tetraploid, and contained chromosomal mutations or chimeras. In total, there were about 15% of polyploids and plants carrying chromosomal aberrations (translocations, inversions) among DH1 individuals. The changes in chromosome number and structure were the main source of observed variation. The level of gene mutation induced in vitro was relatively low. No more than 1% of microspore-derived plants expressed visible morphological changes in DH2 progeny. Only two morphological variants derived from the Bruce cultivar proved to be homozygous mutants (dwarf type) stable up the to third generation. The frequency of DH plants carrying chlorophyll mutation was 5.8%, but most of them (82%) were chimeric and had only a small mutation sector. The level of gametoclonal variation depended on the donor plant genotype. The highest proportion of variants and mutations was observed among DH plants derived from the Bruce cultivar, while the lowest was recorded among plants regenerated from anther culture of the doubled haploid line H930-36. Mechanisms leading to the observed variation and implications resulting from the presented experiments concerning implementation of anther culture in barley breeding were discussed. It was concluded that this method resulted in a high frequency of spontaneous doubling, a low frequency of genetic changes, and being less time and effort-consuming than the ’Bulbosum’ technique, can be applied to most barley breeding programs.
Źródło:: Journal of Applied Genetics; 1997, 38, 4; 437-452
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Anther culture response and genetic relationships between Iranian and European barley (Hordeum vulgare L.) cultivars
Autorzy:: Izadi, S.
Cheghamirza, K.
Kahrizi, D.
Powiązania:: https://bibliotekanauki.pl/articles/80731.pdf
Data publikacji:: 2017
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: barley
Hordeum vulgare
cereal grain
anther culture response
callus induction
genetic diversity
inter-simple sequence repeat marker
Mantel test
Iranian barley
European barley
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2017, 98, 4
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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