Temat: bacterial adhesion - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: The influence of PU foams modification on the efficiency of Citrobacter freundii cells immobilization
Autorzy:: Mizielińska, Małgorzata
Kowalska, Urszula
Łopusiewicz, Łukasz
Powiązania:: https://bibliotekanauki.pl/articles/1178364.pdf
Data publikacji:: 2017
Wydawca:: Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:: Citrobacter freundii
bacterial adhesion
bioimmobilization
polyurethane foam
Opis:: The purpose of the study was to modify PU (polyurethane) foams to increase the efficiency of Citrobacter freundii cells adhesion. The immobilization can contribute to better productivity during the bioconversion of glycerol to 1,3-propanediol (1,3-PD). The results of the study showed that C. freundii immobilized on foams with organic additives could decompose more glycerol and produce more 1,3-PD than cells immobilized on pure foams. The immobilization made production in long-term operations or repeated runs possible. The results of the experiments have also proved that the foams with additives, especially with wood chips and peanut shells, would be the most suitable carriers because they allow the immobilization of a higher number of bacterial cells. It was demonstrated that the initial number of C. freundii cells in all samples (before incubation) was 1.2×106 CFU/mL. The results showed that the accumulation of bacterial cells in the control sample with pure PUF (polyurethane foams) was 1.5×107 CFU/mL after 24 hours of incubation (the number of bacterial cells attached to surface of foams). It was determined that the accumulation of bacterial cells in samples with 15% of additives at 30 °C increased to 2.17×107/ 3.37×107/ 1.25×108/ 1.65×108/ 5.65×107 CFU (PUFbtb, PUFab, PUFwch, PUFps, PUFrc) CFU/mL after 24 hours of incubation.
Źródło:: World Scientific News; 2017, 77, 2; 211-225
2392-2192
Pojawia się w:: World Scientific News
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Measurement of bacterial adhesion to metal surfaces with different chemical composition - evaluation of different methods
Autorzy:: Żywicka, A.
Fijałkowski, K.
Powiązania:: https://bibliotekanauki.pl/articles/363248.pdf
Data publikacji:: 2017
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:: bacterial adhesion
metals
direct method
indirect method
Opis:: In the current study attention has been focused on the evaluation of different methods of measurement of bacterial adhesion to the metal surfaces with different chemical composition. The direct methods, which included determination of the number of bacterial cells using fluorescence microscopy and Colony Forming Units (CFU) on agar medium, and indirect methods using Alamar Blue (AB) and MTT assays, were evaluated. The chemical compositions of the metal surfaces included: copper, iron, chromium and nickel. Interaction effects of assay and metal compound have been specifically demonstrated in this study. It was found that metal ions reacted with components of the indirect colorimetric tests used in his study (AB and MTT assay). Consequently, those tests gave positive false results. In contrast to the indirect methods, direct counting methods such as microscopy techniques and CFU counting, were successfully applied for evaluation of bacterial adhesion to the metal surfaces. However, considering the limits for the surfaces of the examined samples for microscopy, the determination of the CFU was found to be the best method for testing the adhesion to metal surfaces. The method, combined with the appropriate detachment procedures allowed for a precise determination of the number of bacteria on the entire surface of the evaluated metal samples.
Źródło:: Environmental Biotechnology; 2017, 13, 2; 14-22
1734-4964
Pojawia się w:: Environmental Biotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Ultrasound conditioning of Streptococcus thermophilus CNRZ 447: growth, biofilm formation, exopolysaccharide production, and cell membrane permeability
Autorzy:: Khadem, H.
Tirtouil, A.M.
Drabo, M.S.
Boubakeur, B.
Powiązania:: https://bibliotekanauki.pl/articles/2096999.pdf
Data publikacji:: 2020
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Streptococcus thermophilus
ultrasound conditioning
bacterial adhesion
biofilm
exopolysaccharides
Opis:: Sonication is one of the new and innovative approaches that is being increasingly used in food industry to control fermentation processes and to eradicate spoiling. Recently, this approach has seen new industrial applications such as enhancing microbial productivity. The present study aimed to assess the effects of ultrasound conditioning on the metabolism and extracellular matrix production of Streptococcus thermophilus. Bacterial suspensions were treated in ultrasonic bath (35 kHz, 240/60 W peak/nominal power, 1.8 l capacity) for different time periods (5, 10, 15, 20, 30, 45, and 65 min), and the growth improvement, adhesion ability, biofilm formation, and exopolysaccharide production of the bacterial strain were measured. The bacterial strain exhibited resistance to the treatment, and the conditioning improved the growth, adhesion, membrane permeability, biofilm formation, and exopolysaccharide production ability. An optimal treatment was obtained for 30 minutes of conditioning. An excellent yield of desirable exopolysaccharides (1788 mg glucose equivalent/l) was achieved. Ultrasound conditioning may be used as a potential approach to enhance certain biotechnological properties of industrial microorganisms.
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 2; 159-165
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Poszukiwanie prostych modeli do badania adhezji bakterii probiotycznych
Searching for simple models to study the adhesion of probiotic bacteria
Autorzy:: Czaczyk, K
Olejnik, A
Miezal, P
Grajek, W
Powiązania:: https://bibliotekanauki.pl/articles/828476.pdf
Data publikacji:: 2005
Wydawca:: Polskie Towarzystwo Technologów Żywności
Tematy:: bakterie probiotyczne
adhezja bakterii
Lactobacillus casei
Lactobacillus acidophilus
Lactobacillus rhamnosus
metody badan
alginiany
karageny
kolagen
karboksymetyloceluloza
zdolnosci adhezyjne
probiotic bacteria
bacterial adhesion
test method
alginate
carrageenan
carboxymethyl cellulose
adhesive ability
Opis:: Przeprowadzono studia porównawcze nad możliwością zastąpienia w badaniach adhezji bakterii mlekowych modelu kultury komórek nabłonka jelitowego innymi uproszczonymi modelami. Badano przyczepność bakterii probiotycznych Lactobacillus casei Shirota ATCC 39539, Lactobacillus acidophilus LC1 oraz Lactobacillus rhamnosus GG ATCC 53103 do membran wykonanych z alginianu, karagenu, żelatyny, kolagenu, szkła, polistyrenu i karboksymetylocelulozy. Zaobserwowano największy stopień przyczepności bakterii probiotycznych do membran wykonanych z karboksymetylocelulozy i kolagenu. W większości wariantów doświadczeń liczba przyczepionych komórek bakteryjnych wzrastała wraz ze wzrostem czasu ich kontaktu z powierzchnią stałą. Wykazano także różnice w przyczepności komórek do hydrożeli o różnych stężeniach. Na podstawie przeprowadzonych badań można stwierdzić, że stopień adhezji bakterii probiotycznych do różnych powierzchni organicznych i nieorganicznych jest cechą zaleną od szczepu mikroorganizmu. Żaden z prostych modeli adhezyjnych, zastosowanych w tej pracy, nie charakteryzował się adhezyjnością porównywalną z komórkami nabłonka jelitowego Caco-2 i HT-29, stosowanego obecnie jako główny model in vitro do badań adhezji bakterii probiotycznych.
Comparative studies were performed on the possibility of replacing intestinal epithelial cells by some other simplified models for the purpose of examining the adhesion of probiotic bacteria. The adhesion of Lactobacillus casei Shirota ATCC 39539, Lactobacillus acidophilus LC1, and Lactobacillus rhamnosus GG ATCC 53103 to surfaces of alginate, carrageenan, gelatin, collagen, glass, polystyrene, carboxymethylcellulose, and to intestinal epithelial cells (Caco-2 and HT-29 lines) were tested. Among the examined solid surfaces, the highest degree of adhesion was observed in the case of carboxymethylcellulose and collagen. In the majority of experimental variants, the number of bacterial cells adhering to surfaces increased parallel to the time of their contacting a solid surface. Additionally, there were stated differences in the cell adhesion to hydrogels showing varying concentration rates. On the basis of the experiments performed, it is possible to state that the adhesion degree of probiotic bacteria to various inorganic and organic surfaces is a parameter that depends on the strain of a microorganism. Among all the simplified models applied in the studies in question, no one was marked by an adhesion rate comparable to the adhesion of intestinal epithelial cells Caco-2 and HT-29, which are, nowadays, used as a major model in vitro while investigating the adhesion of probiotic bacteria.
Źródło:: Żywność Nauka Technologia Jakość; 2005, 12, 1; 84-96
1425-6959
Pojawia się w:: Żywność Nauka Technologia Jakość
Dostawca treści:: Biblioteka Nauki

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