Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

English (EN)·Polski (PL)·Yкраїнський (UK)
Przejdź do strony domowej biblioteki Centralna Biblioteka Wojskowa Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaCentralna Biblioteka Wojskowa

Wyszukujesz frazę "microscopy" wg kryterium: Temat

  Lista filtrów
Wyrównaj
Wyświetlanie 1-15 z 26
Tytuł:
Microscopic analysis of the nanostructures impact on endothelial cells
Autorzy:
Kołodziejczyk, Agnieszka Maria
Kucińska, Magdalena
Jakubowska, Aleksandra
Siatkowska, Małgorzata
Sokołowska, Paulina
Kotarba, Sylwia
Makowski, Krzysztof
Komorowski, Piotr
Walkowiak, Bogdan
Powiązania:
https://bibliotekanauki.pl/articles/1844983.pdf
Data publikacji:
2020
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
nanostructures
atomic force microscopy
transmission electron microscopy
scanning electron microscopy
cell morphology
Opis:
Nowadays nanostructures are more and more often designed as carriers for drug delivery, especially to improve the drug pharmacokinetics and pharmaco-dynamics. Numerous kinds of nanostructures are considered a good prospect for medical applications thanks to their small size, acceptable biocompatibility and toxicity. Due to the fact that nanotechnology is a new field of science, every nano-scale product must be thoroughly examined regarding its toxicity to the human body. This study provides new insights into effects of exposing endothelial cells to the selected nanostructures. Dendrimers of the fourth generation (PAMAMs), multi-walled carbon nanotubes (MWCNTs) and silver nanoparticles (SNPs) were used to evaluate nanostructures influence on endothelial cells in vitro. The nanostructures were evaluated via transmission electron microscopy and dynamic light scattering technique. The cells previously exposed to the nanostructures were observed and analyzed via the atomic force microscopy and scanning electron microscopy to obtain a quantitative evaluation of the cells morphology. The presence of multi-walled carbon nanotubes and silver nanoparticles on the cells surface was confirmed by the scanning electron microscopy. Our results confirm that the surface association and/or uptake of nanostructures by the cells resulting from physicochemical and biological processes, affect the cells morphology. Morphological changes can be induced by the membrane proteins interaction with nanomaterials, which trigger a sequence of intracel-lular biological processes.
Źródło:
Engineering of Biomaterials; 2020, 23, 154; 2-8
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microscopic analysis of the nanostructures impact on endothelial cells
Autorzy:
Kołodziejczyk, Agnieszka Maria
Kucińska, Magdalena
Jakubowska, Aleksandra
Siatkowska, Małgorzata
Sokołowska, Paulina
Kotarba, Sylwia
Makowski, Krzysztof
Komorowski, Piotr
Walkowiak, Bogdan
Powiązania:
https://bibliotekanauki.pl/articles/970974.pdf
Data publikacji:
2020
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
nanostructures
atomic force microscopy
transmission electron microscopy
scanning electron microscopy
cell morphology
Opis:
Nowadays nanostructures are more and more often designed as carriers for drug delivery, especially to improve the drug pharmacokinetics and pharmaco-dynamics. Numerous kinds of nanostructures are considered a good prospect for medical applications thanks to their small size, acceptable biocompatibility and toxicity. Due to the fact that nanotechnology is a new field of science, every nano-scale product must be thoroughly examined regarding its toxicity to the human body. This study provides new insights into effects of exposing endothelial cells to the selected nanostructures. Dendrimers of the fourth generation (PAMAMs), multi-walled carbon nanotubes (MWCNTs) and silver nanoparticles (SNPs) were used to evaluate nanostructures influence on endothelial cells in vitro. The nanostructures were evaluated via transmission electron microscopy and dynamic light scattering technique. The cells previously exposed to the nanostructures were observed and analyzed via the atomic force microscopy and scanning electron microscopy to obtain a quantitative evaluation of the cells morphology. The presence of multi-walled carbon nanotubes and silver nanoparticles on the cells surface was confirmed by the scanning electron microscopy. Our results confirm that the surface association and/or uptake of nanostructures by the cells resulting from physicochemical and biological processes, affect the cells morphology. Morphological changes can be induced by the membrane proteins interaction with nanomaterials, which trigger a sequence of intracel-lular biological processes.
Źródło:
Engineering of Biomaterials; 2020, 23, 154; 2-8
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The impact of the light exposure on the morphological properties of selected photoresists
Autorzy:
Sikora, Andrzej
Janus, Paweł
Sierakowski, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/174640.pdf
Data publikacji:
2019
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
photolitography
polymer degradation
atomic force microscopy
Opis:
In this paper we present the investigation aimed at the photoresist roughness change determination as a reliable estimator of the exposition rate in the processing verification in semiconductor industry. By employing atomic force microscopy as the 3D high resolution surface imaging tool, we tested twelve popular photoresists in terms of the morphological properties changes, while the following radiation doses were applied. Basing on high precision, and repetitive sample positioning, it was possible to perform the tests with high degree of confidence and observe the roughness change dynamics. Various profiles of roughness changes were observed, showing the need for individual study of each material. Moreover, it was possible to select the photoresists which due to poor homogeneity and small roughness changes are not suitable to such a verification. According to our knowledge, no such study was performed so far.
Źródło:
Optica Applicata; 2019, 49, 1; 177-185
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ion Beam Induced Surface Modification of ta-C Thin Films
Autorzy:
Berova, M.
Sandulov, M.
Tsvetkova, T.
Kitova, S.
Bischoff, L.
Boettger, R.
Powiązania:
https://bibliotekanauki.pl/articles/1033772.pdf
Data publikacji:
2017-08
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
carbon
ion implantation
atomic force microscopy
Opis:
Thin film samples (d ≈40 nm) of tetrahedral amorphous carbon (ta-C), deposited by filtered cathodic vacuum arc, were implanted with Ga⁺ at ion energy E =20 keV and ion fluences D=3×10¹⁴-3×10¹⁵ cm¯² and N⁺ with the same energy and ion fluence D=3×10¹⁴ cm¯². The Ga⁺ ion beam induced surface structural modification of the implanted material, displayed by formation of new phase at non-equilibrium condition, which could be accompanied by considerable changes in the optical properties of the ta-C films. The N⁺ implantation also results in modification of the surface structure. The induced structural modification of the implanted material results in a considerable change of its topography and optical properties. Nanoscale topography and structural properties characterisation of the Ga⁺ and N⁺ implanted films were performed using atomic spectroscopy analysis. The observed considerable surface structural properties modification in the case of the higher fluence Ga⁺ implanted samples results from the relatively high concentration of introduced Ga⁺ atoms, which is of the order of those for the host element.
Źródło:
Acta Physica Polonica A; 2017, 132, 2; 299-301
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In situ-formed bacterial exopolysaccharide (EPS) as a potential carrier for anchorage-dependent cell cultures
Autorzy:
Komorowski, Piotr
Kołodziejczyk, Agnieszka
Makowski, Krzysztof
Kotarba, Sylwia
Walkowiak, Bogdan
Powiązania:
https://bibliotekanauki.pl/articles/1844871.pdf
Data publikacji:
2021
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
bacterial exopolysaccharides
dextran- -based “microcarriers”
scanning electron microscopy
atomic force microscopy
roughness parameters
three-dimensional cell culture
Opis:
The study involved the use of a bacterial strain isolated from environmental samples which produce the biopolymer in the form of pellets in the submerged culture. This material (bacterial exopolysaccharide) is produced by bacteria of the Komogateibacter xylinus which are prevalent in the environment. The aim of this study was to characterize bacterial exopolysaccharides and commercial dextran-based “microcarriers” in terms of their roughness and cell culture effects, including the morphology and viability of the human hybridoma vascular endothelial cell line EA.hy926. The pellets were characterized using scanning electron microscopy (SEM) and atomic for¬ce microscopy (AFM). The resulting structures were used for cell culture of adherent cells (anchorage¬-dependent cells). At the same time, the cultures with commercial, dextran-based “microcarriers” were carried out for comparative purposes. After com¬pletion of the cell culture (24 hours of culture), the cellulose and commercial “carriers” were analyzed using SEM and AFM. Finally, the obtained cell dens¬ities (fluorescence labelling) and their morphological characteristics (SEM) were compared. The obtained results strongly support the applicability of bacterial exopolysaccharide (EPS) in tissue engineering to build innovative 3D scaffolds for cell culture, the more so that it is technologically possible to produce EPS as spatially complex structure
Źródło:
Engineering of Biomaterials; 2021, 24, 159; 18-23
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Surface analysis of long-term hemodialysis catheters made of carbothane (poly(carbonate)urethane) before and after implantation in the patients’ bodies
Autorzy:
Nycz, M.
Paradowska, E.
Arkusz, K.
Kudliński, B.
Krasicka-Cydzik, E.
Powiązania:
https://bibliotekanauki.pl/articles/306579.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
chropowatość powierzchni
mikroskopia sił atomowych
mikroskopia skaningowa
surface roughness
atomic force microscopy
scanning electron microscopy
Catheter-tissue contact
film
Opis:
The vascular cannulation is associated with a number of complications. The aim of this work was to study the composition and distribution of the film covering the surfaces of Mahurkar Maxid and Palindrome catheters, which were removed from the body of long-term hemodialysis patients. Moreover, the roughness and contact angle of the catheters were evaluated. Methods: Two brand new (as a reference) and thirty used catheters were the subject of the study. Their implantation period lasted from 4 months to a year and the reason for removal was the production of another vascular access or obstruction. Surfaces were analyzed by scanning electron microscope, atomic force microscope and goniometer. Results: The inner surfaces of the used catheters were covered with a film of various complexity which includes a plurality of protein, blood cell counts and the crystals. The closer to the distal part the film becomes more complex and multi-layered. Even the surfaces of brand new catheter were not completely smooth. The only significant difference between analyzed models was the presence of thrombus in the distal part of Mahurkar Maxid catheters, not in the Palindrome. Conclusions: The distal part of the catheters is the place most exposed to obstruction and infection, which may be due to not reaching the anticoagulant agent into this part. Not only the occurrence of side holes affects the formation of thrombus, but also their quantity, geometry and distribution which effect on fluid mechanics. The surface of the catheters needs to improvement to minimize the occurrence of defects and cracks.
Źródło:
Acta of Bioengineering and Biomechanics; 2018, 20, 2; 47-53
1509-409X
2450-6303
Pojawia się w:
Acta of Bioengineering and Biomechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Diffuse-layer surface potentials of colemanites mined in Turkey
Autorzy:
Senol-Arslan, Dilek
Drelich, Jaroslaw W.
Powiązania:
https://bibliotekanauki.pl/articles/2146938.pdf
Data publikacji:
2022
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
colemanite
surface potential
atomic force microscopy
boron
DLVO
Opis:
Colemanite crystal specimens were handpicked at Kestelek, Emet (Hisarcik, Espey), Bigadic mines in Turkey for characterization of their composition and surface potential. X-ray diffraction analysis revealed no differences in mineralogical makeup of the crystals, but elemental analysis indicated differences in the type of trace (<0.1 wt%) elements. Zeta potential measurements showed small differences in zeta potential values, with isoelectric points (iep) varying from about pH = 9.6 to pH = 10.2. However, no correlation was found between iep and the type of trace elements. Additionally, atomic force microscopy (AFM) was employed to measure the colloidal interactions between a silicon nitride (Si3N4) cantilever tip and colemanite crystal surfaces in 1 wt% colemanite-saturated aqueous solutions at three different pHs (8.4, 9.4 and 11). The Derjaguin-Landau-Verwey-Overbeek theory (DLVO) was applied to examine the AFM tip interactions with colemanite surfaces in an aqueous solution of colemanite saturated solutions. The results revealed attractive forces at pH = 8.4 and 9.4 and repulsive forces at pH = 11, confirming the location of an isoelectric point for colemanite specimens somewhere between pH = 9.5 and pH = 10.1. Theoretical analysis of the force curves using the DLVO theory allowed for assessment of both surface charge density and surface potential for colemanite specimens used in this study.
Źródło:
Physicochemical Problems of Mineral Processing; 2022, 58, 5; art. no. 151933
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Atomic force microscopy studies of the adhesive properties of DPPC vesicles containing β-carotene
Autorzy:
Augustyńska, Dominika
Jemioła-Rzemińska, Małgorzata
Burda, Kvetoslava
Strzałka, Kazimierz
Powiązania:
https://bibliotekanauki.pl/articles/1039795.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
atomic force microscopy
dipalmitoylphosphatidylcholine
β-carotene
liposome adhesion
thermal transition
Opis:
A role of carotenoids as modulators of physical properties of model and biological membranes has been already postulated. However, there is a lack of information on the influence of these pigments on interactions between the lipids which form such membranes. This paper applies atomic force microscopy (AFM) in to study the effects of β-carotene on the adhesion properties of DPPC multilamellar liposomes. This allowed us to gain, for the first time, a direct insight into the interactions between the components in model systems on a molecular level. We observe that the adhesive forces in DPPC multilamellar liposomes containing 1mol% of β-carotene decrease exponentially with increasing temperature, and that at about 37°C they diminish. In the case of pure liposomes the decline in adhesion is of a different nature and the adhesive forces disappear at 34°C. The adhesive forces are about 5 times higher at 31°C in the presence of β-carotene than in its absence. However, measurements using differential scanning calorimetry (DSC) showed a shift of the lamellar-to-undulled-lamellar phase transition toward lower temperatures by about 0.8±0.2°C in a system containing β-carotene. The enthalpy changes (ΔH) of this transition are similar for both systems. For the main transition, gel-to-liquid crystalline, the peak is shifted by about 0.5±0.1°C, and ΔH decreases by about 30% in liposomes treated with β-carotene in comparison to pure liposomes. Our results suggest increased cooperation between liposome components in a system with enriched β-carotene, which cause a change in phase transition temperatures. Moreover, these interactions are very sensitive to temperature.
Źródło:
Acta Biochimica Polonica; 2012, 59, 1; 125-128
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nanoindentation and atomic force microscopy of thin surface layers formed on the shaft surface after burnishing process
Autorzy:
Kuznetsova, T.
Zubar, T.
Chizik, S.
Miszczak, A.
Labuda, W.
Powiązania:
https://bibliotekanauki.pl/articles/242839.pdf
Data publikacji:
2016
Wydawca:
Instytut Techniczny Wojsk Lotniczych
Tematy:
microstructure
atomic force microscopy
nanoindentation
microhardness
Young's modulus
burnishing process
Opis:
The article presents the experimental results of investigation of the pumps shaft surface made of stainless steel after burnishing process. The process of burnishing shafts proposed here aims at increasing the service durability of marine pump shafts of seawater installations, which should give economic benefits in comparison with traditional methods. Burnishing process enables the achievement of high smoothness of machined surface together with the surface layer hardening. This process has been performed in industrial experience on universal machine tools and on CNC machines but it is regarded as plastic tooling. Therefore, the final formation of dimensions and service properties with the use of burnishing constitutes a chipless and dustless treatment, which allows for ranking burnishing among ecological tooling methods. The properties of the surface layers determine the tribological properties of the shaft. The methods of scanning electron microscopy (SEM), atomic force microscopy (AFM) and nanoindentation (NI) were used. The morphology of the changed layers formed by plastic deforming of steel during burnishing treatment was investigated on micro- and nanolevel by SEM and AFM after 1, 2, 3 and 4 passed of burnishing tool. The values of microhardness and Young´s modulus were measured on the shaft cross section from the surface till the 50 – 300 μm depth. The presence of the soft changed layer on the hardened shaft surface after burnishing process determines the decrease of the coefficient of friction during exploitation.
Źródło:
Journal of KONES; 2016, 23, 3; 303-310
1231-4005
2354-0133
Pojawia się w:
Journal of KONES
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Selected imaging techniques applied in forensic science
Wybrane techniki obrazowania stosowane w kryminalistyce
Autorzy:
Łasińska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/501727.pdf
Data publikacji:
2018-03-15
Wydawca:
Agencja Bezpieczeństwa Wewnętrznego
Tematy:
imaging techniques
microscopy
SEM
X-ray microanalysis
EDS
Raman spectroscopy
atomic force microscopy
AFM
techniki obrazowania
mikroskopia
mikroanaliza rentgenowska
spektroskopia Ramana
mikroskopia sił atomowych
Opis:
The article presents issues that include various imaging techniques used in forensics. Technology development observed in the modern world, implies changes in all areas of life, improving, speeding up and opening them to new research capabilities. The use of advanced technology allows precise analysis of the evidence. The opportunity to observe different samples at high magnification is necessary in the performance of their documentation (examination of the morphology of the surface, setting the diagnostic characteristics). The application of optical microscopy, electron microscopy or atomic force microscopy, and integrated spectroscopic systems is an essential and powerful tool for implementation observations of a variety of materials.
W artykule przedstawiono zagadnienia obejmujące różne techniki obrazowania wykorzystywane w kryminalistyce. Rozwój technologii obserwowany we współczesnym świecie pociąga za sobą zmiany we wszystkich dziedzinach życia, usprawniając, przyspieszając oraz otwierając je na nowe możliwości badawcze. Wykorzystanie zaawansowanej technologii pozwala na precyzyjną analizę materiału dowodowego. Możliwość obserwacji różnych próbek w dużych powiększeniach jest niezbędna przy wykonywaniu ich dokumentacji (badanie morfologii powierzchni, ustalanie cech diagnostycznych). Zastosowanie mikroskopii optycznej, elektronowej lub sił atomowych, a także zintegrowanych z nimi systemów spektroskopowych jest podstawowym, a zarazem potężnym narzędziem służącym wykonywaniu obserwacji różnorodnych materiałów.
Źródło:
Przegląd Bezpieczeństwa Wewnętrznego; 2018, 10, 18; 283-309
2080-1335
2720-0841
Pojawia się w:
Przegląd Bezpieczeństwa Wewnętrznego
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wybrane techniki obrazowania stosowane w kryminalistyce.
Selected imaging techniques applied in forensic science
Autorzy:
Łasińska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/501703.pdf
Data publikacji:
2018-03-15
Wydawca:
Agencja Bezpieczeństwa Wewnętrznego
Tematy:
techniki obrazowania
mikroskopia
SEM
mikroanaliza rentgenowska
EDS
spektroskopia Ramana
mikroskopia sił atomowych
AFM
imaging techniques
microscopy
X-ray microanalysis
Raman spectroscopy
atomic force microscopy
Opis:
W artykule przedstawiono zagadnienia obejmujące różne techniki obrazowania wykorzystywane w kryminalistyce. Rozwój technologii obserwowany we współczesnym świecie pociąga za sobą zmiany we wszystkich dziedzinach życia, usprawniając, przyspieszając oraz otwierając je na nowe możliwości badawcze. Wykorzystanie zaawansowanej technologii pozwala na precyzyjną analizę materiału dowodowego. Możliwość obserwacji różnych próbek w dużych powiększeniach jest niezbędna przy wykonywaniu ich dokumentacji (badanie morfologii powierzchni, ustalanie cech diagnostycznych). Zastosowanie mikroskopii optycznej, elektronowej lub sił atomowych, a także zintegrowanych z nimi systemów spektroskopowych jest podstawowym, a zarazem potężnym narzędziem służącym wykonywaniu obserwacji różnorodnych materiałów.
The article presents issues that include various imaging techniques used in forensics. Technology development observed in the modern world, implies changes in all areas of life, improving, speeding up and opening them to new research capabilities. The use of advanced technology allows precise analysis of the evidence. The opportunity to observe different samples at high magnification is necessary in the performance of their documentation (examination of the morphology of the surface, setting the diagnostic characteristics). The application of optical microscopy, electron microscopy or atomic force microscopy, and integrated spectroscopic systems is an essential and powerful tool for implementation observations of a variety of materials.
Źródło:
Przegląd Bezpieczeństwa Wewnętrznego; 2018, 10, 18; 89-119
2080-1335
2720-0841
Pojawia się w:
Przegląd Bezpieczeństwa Wewnętrznego
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of epoxy functional silanes in the preparation of DNA microarrays
Autorzy:
Frydrych-Tomczak, E.
Uszczynska, B.
Ratajczak, T.
Markiewicz, W.T.
Figlerowicz, M.
Nowicki, M.
Maciejewski, H.
Chmielewski, M.K.
Powiązania:
https://bibliotekanauki.pl/articles/80900.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
DNA microarray
gene expression
organofunctional silane
oligonucleotide
atomic force microscopy
goniometer
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2014, 95, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cartilage tissue examination using atomic force microscopy
Autorzy:
Paluch, Jarosław
Markowski, Jarosław
Pilch, Jan
Smółka, Wojciech
Jasik, Krzysztof Piotr
Kilian, Filip
Likus, Wirginia
Bajor, Grzegorz
Chrobak, Dariusz
Glowka, Karsten
Starczewska, Oliwia
Powiązania:
https://bibliotekanauki.pl/articles/27324036.pdf
Data publikacji:
2022
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
atomic force microscopy
cartilage
biopolymers
chondrocytes
intercellular matrix
mikroskop
biopolimery
tkanka
Opis:
Life sciences, a field closely intertwined with human biology and physiology, employ various research methods, including morphology studies and quantitative analysis through non-destructive techniques. Biological specimens often consist of three-phase structures, characterized by the presence of gas, liquid, and solid components. This becomes crucial when the chosen research methodology requires the removal of water from samples or their transfer to a cryostat. In the current research, mechanical and topographical examination of cartilage was performed. The materials were generously provided by the Department of Anatomy at the Medical University of Silesia, thereby eliminating any concerns regarding their origin or ethical use for scientific purposes. Our research methodology involved the application of atomic force microscopy (AFM), which minimally disrupts the internal equilibrium among the aforementioned phases. Cartilage, recognized as a ‘universal support material’ in animals, proves to be highly amenable to AFM research, enabling the surface scanning of the examined material. The quantitative results obtained facilitate an assessment of the internal structure and differentiation of cartilage based on its anatomical location (e.g., joints or ears). Direct images acquired during the examination offer insights into the internal structure of cartilage tissue, revealing morphological disparities and variations in intercellular spaces. The scans obtained during the measurements have unveiled substantial distinctions, particularly in the intercellular ‘essence’, characterized by granularities with a diameter of approximately 0.5 μm in ear cartilage and structural elements in articular cartilage measuring about 0.05 μm. Thus, AFM can be a valuable cognitive tool for observing biological samples in the biological sciences, particularly in medicine (e.g. clinical science).
Źródło:
Engineering of Biomaterials; 2022, 25, 167; 17--23
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dynamic force measurements of avidin-biotin and streptavdin-biotin interactions using AFM
Autorzy:
de Odrowąż Piramowicz, Marzena
Czuba, Paweł
Targosz, Marta
Burda, Kvĕtoslava
Szymoński, Marek
Powiązania:
https://bibliotekanauki.pl/articles/1041274.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
atomic force microscopy
rupture force
loading rate
streptavidin
avidin
biotin
dissociation rate
Opis:
Using atomic force microscopy (AFM) we performed dynamic force measurements of the adhesive forces in two model systems: avidin-biotin and streptavidin-biotin. In our experiments we used glutaraldehyde for immobilization of (strept)avidin on the tip and biotin on the sample surface. Such interface layers are more rigid than those usually reported in the literature for AFM studies, when (strept)avidin is coupled with biotinylated bovine albumin and biotin with agarose polymers. We determined the dependence of the rupture forces of avidin-biotin and streptavidin-biotin bonds in the range 300-9600 pN/s. The slope of a semilogarithmic plot of this relation changes at about 1700 pN/s. The existence of two different regimes indicates the presence of two activation barriers of these complexes during the dissociation process. The dissociation rates and activation energy barriers, calculated from the Bell model, for the avidin-biotin and streptavidin-biotin interactions are similar to each other for loading rates >1700 pN/s but they are different from each other for loading rates < 1700 pN/s. In the latter case, the dissociation rates show a higher stability of the avidin-biotin complex than the streptavidin-biotin complex due to a larger outer activation barrier of 0.8 kBT. The bond-rupture force is about 20 pN higher for the avidin-biotin pair than for the streptavidin-biotin pair for loading rates < 1700 pN/s. These two experimental observations are in agreement with the known structural differences between the biotin binding pocket of avidin and of streptavidin.
Źródło:
Acta Biochimica Polonica; 2006, 53, 1; 93-100
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Multiscale water drop contact angles at selected silica surfaces
Autorzy:
Zhang, Chen
Wang, Xuming
Li, Lixia
Jin, Jiaqi
Polson, Randy
Miller, Jan D.
Powiązania:
https://bibliotekanauki.pl/articles/2146924.pdf
Data publikacji:
2022
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
contact angle
atomic force microscopy
hollow tip
submicron-drop
wettability
MD simulation
Opis:
In this study, multiscale advancing contact angles for glycerol/water drops at silica surfaces are reported for millidrops, submicron-drops, and nanodrops. Selected silica surfaces were muscovite, silicon, and talc. The contact angles for millidrops (1–2 mm) were determined by the traditional sessile drop technique. For submicron-drops (0.1–1.0 μm), a hollow tip Atomic Force Microscope (AFM) procedure was used. The contact angles for nanodrops (~7 nm) were examined from Molecular Dynamics (MD) simulation. The results were compared to evaluate the effect of drop size on the contact angle. In the case of the hydrophobic talc surface, the 75° advancing contact angle did not vary significantly with drop size. For the hydrophilic muscovite surface, the water drop wet the surface and an advancing contact angle of about 10° was found for the millidrops and submicron-drops. However, for the MD simulated nanodrops, attachment and spreading of the ~7 nm drop created a 2D film of molecular dimensions, the contact angle of which was difficult to define and varied from 0° to 17°. Perhaps of equal interest from the MD simulation results was that the spreading of the glycerol/water nanodrop at the muscovite surface resulted in crystallographic directional transport of water molecules to the extremities of the 2D film. Such separation and segregation left the center of the film with an increased concentration of glycerol. Based on these results, the line tension, which has been found in other investigations to account for contact angle decrease with a decrease in drop size, does not seem to be a significant factor in this study.
Źródło:
Physicochemical Problems of Mineral Processing; 2022, 58, 5; art. no. 152154
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 26

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies