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    Wyświetlanie 1-4 z 4
    Tytuł:
    Współczesne kierunki w otrzymaniu i badaniu antygenów pasożytów służących do immunodiagnostyki
    Contemporary trends in obtaining and studying parasite antigens used in immunodiagnostics
    Autorzy:
    Machnicka, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/837517.pdf
    Data publikacji:
    1988
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parazytologia
    pasozyty
    antygeny
    otrzymywanie
    kierunki badan
    immunodiagnostyka
    immunoparazytologia
    Opis:
    The contemporary methods of specific antigen selection as well as determination of particular for infected hosts epitopes were described. The immuno-diagnostics of echinococcosis, trichinellosis, canine filariosis and toxocarosis (larva migrans) were demonstrated as examples to illustrate the progress in test efficiency. The need of application of highly specific antigens in very sensitive methods, to which ELISA belongs, was stressed.
    Źródło:
    Annals of Parasitology; 1988, 34, 4-6
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Współczesne kierunki w otrzymaniu i badaniu antygenów pasożytów służących do immunodiagnostyki
    Contemporary trends in obtaining and studying parasite antigens used in immunodiagnostics
    Autorzy:
    Machnicka, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/2152676.pdf
    Data publikacji:
    1988
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parazytologia
    pasozyty
    antygeny
    otrzymywanie
    kierunki badan
    immunodiagnostyka
    immunoparazytologia
    Opis:
    The contemporary methods of specific antigen selection as well as determination of particular for infected hosts epitopes were described. The immuno-diagnostics of echinococcosis, trichinellosis, canine filariosis and toxocarosis (larva migrans) were demonstrated as examples to illustrate the progress in test efficiency. The need of application of highly specific antigens in very sensitive methods, to which ELISA belongs, was stressed.
    Źródło:
    Wiadomości Parazytologiczne; 1988, 34, 4-6; 477-483
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wpływ procedury otrzymywania antygenów ekskrecyjno-sekrecyjnych Trichinella spiralis na wynik testu ELISA u zarażonych świń
    The influence of the procedure of excretory -secretory L1 Trichinella spiralis antigen preparation on the efficiency of an ELISA test in pigs
    Autorzy:
    Bień, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2144343.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    analiza statystyczna
    diagnostyka weterynaryjna
    pasozyty
    nicienie
    Warszawa konferencja
    otrzymywanie
    procedury
    trzoda chlewna
    zwierzeta chore
    parazytologia
    Trichinella spiralis
    konferencje
    antygeny
    wartosc diagnostyczna
    test ELISA
    Opis:
    Background. Trichinellosis is a parasitic zoonosis transmitted to humans by consumption of raw or undercooked meat from animals infected by worms of the Trichinella genus. Every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease in Poland. The usefulness of ELISA for anti-T. spiralis IgG detection in pigs is still limited by the nature of antigen. The objective in the present study was to compare the usefulness of excretory-secretory antigens of L1 T. spiralis for the serological detection of IgG antibodies in pigs. Material and methods. The antigens were prepared in different laboratories: Ag ES L1 T. spiralis (N) in Germany, Ag ES L1 T. spiralis (W) in Italy and Ag ES L1 T. spiralis in Poland. Conventional, Iberian pigs were infected with 200, 1000 and 20 000 muscle larvae of T. spiralis. Serum samples were obtained at 5 and 1 dbi (day before infection), and 5, 10, 15, 20, 25, 30, 40, 50, 60 dpi (day post infection) and screened for specific IgG antibodies to excretory-secretory L1 T. spiralis antigens. Serum samples were obtained from the EU project TRICHIPORSE. The cut-off value of ELISA was determined on serum samples from 248 Trichinella-free pigs from Poznań and Boża Wola, that were examined by artificial digestion. Results. In pigs infected with 200 L1 T. spiralis larvae, specific IgG were detectable from 50 dpi, when the Ag ES L1 T. spiralis (N) was used, whereas when Ag ES L1T. spiralis (W) and Ag ES L1 T. spiralis were used, the specific IgG were detectable from 40 dpi. In pigs infected with 1000 L1 T. spiralis larvae, specific IgG was observed from 30 dpi when Ag ES L1 T. spiralis (W) and Ag ES L1 T. spiralis were used, but when Ag ES L1 T. spiralis (N) was used specific IgG were detectable from 40 dpi. In the group infected with the highest dose of T. spiralis larvae, specific IgG were detectable from 30 dpi when Ag ES L1 T. spiralis (N) and Ag ES L1 T. spiralis (W) were used, whereas when Ag ES L1 T. spiralis was used specific IgG were detectable from 20 dpi. The results strongly indicated that in the examined pigs, the specific IgG response against T. spiralis infection is dose dependent. Furthermore, it was shown that the high infectious dose induced earlier increasing of specific IgG response. Statistical analysis revealed a significant positive correlation between OD values obtained in procedures based on the three antigens. The results were statistically repeatable for procedures and for single pigs (P<0.01).
    Źródło:
    Wiadomości Parazytologiczne; 2006, 52, 3; 219-230
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wplyw procedury otrzymywania antygenow ekskrecyjno-sekrecyjnych Trichinella spiralis na wynik testu ELISA u zarazonych swin
    The influence of the procedure of excretory -secretory L1 Trichinella spiralis antigen preparation on the efficiency of an ELISA test in pigs
    Autorzy:
    Bien, J
    Powiązania:
    https://bibliotekanauki.pl/articles/840241.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    analiza statystyczna
    diagnostyka weterynaryjna
    pasozyty
    nicienie
    Warszawa konferencja
    otrzymywanie
    procedury
    trzoda chlewna
    zwierzeta chore
    parazytologia
    Trichinella spiralis
    konferencje
    antygeny
    wartosc diagnostyczna
    test ELISA
    Opis:
    Background. Trichinellosis is a parasitic zoonosis transmitted to humans by consumption of raw or undercooked meat from animals infected by worms of the Trichinella genus. Every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease in Poland. The usefulness of ELISA for anti-T. spiralis IgG detection in pigs is still limited by the nature of antigen. The objective in the present study was to compare the usefulness of excretory-secretory antigens of L1 T. spiralis for the serological detection of IgG antibodies in pigs. Material and methods. The antigens were prepared in different laboratories: Ag ES L1 T. spiralis (N) in Germany, Ag ES L1 T. spiralis (W) in Italy and Ag ES L1 T. spiralis in Poland. Conventional, Iberian pigs were infected with 200, 1000 and 20 000 muscle larvae of T. spiralis. Serum samples were obtained at 5 and 1 dbi (day before infection), and 5, 10, 15, 20, 25, 30, 40, 50, 60 dpi (day post infection) and screened for specific IgG antibodies to excretory-secretory L1 T. spiralis antigens. Serum samples were obtained from the EU project TRICHIPORSE. The cut-off value of ELISA was determined on serum samples from 248 Trichinella-free pigs from Poznań and Boża Wola, that were examined by artificial digestion. Results. In pigs infected with 200 L1 T. spiralis larvae, specific IgG were detectable from 50 dpi, when the Ag ES L1 T. spiralis (N) was used, whereas when Ag ES L1T. spiralis (W) and Ag ES L1 T. spiralis were used, the specific IgG were detectable from 40 dpi. In pigs infected with 1000 L1 T. spiralis larvae, specific IgG was observed from 30 dpi when Ag ES L1 T. spiralis (W) and Ag ES L1 T. spiralis were used, but when Ag ES L1 T. spiralis (N) was used specific IgG were detectable from 40 dpi. In the group infected with the highest dose of T. spiralis larvae, specific IgG were detectable from 30 dpi when Ag ES L1 T. spiralis (N) and Ag ES L1 T. spiralis (W) were used, whereas when Ag ES L1 T. spiralis was used specific IgG were detectable from 20 dpi. The results strongly indicated that in the examined pigs, the specific IgG response against T. spiralis infection is dose dependent. Furthermore, it was shown that the high infectious dose induced earlier increasing of specific IgG response. Statistical analysis revealed a significant positive correlation between OD values obtained in procedures based on the three antigens. The results were statistically repeatable for procedures and for single pigs (P<0.01).
    Źródło:
    Annals of Parasitology; 2006, 52, 3; 219-230
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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