- Tytuł:
-
Badania wplywu chlorfenwinfosu na aktywnosc aminotransferaz osoczowych i watrobowych w warunkach in vitro
In vitro studies on the effects of chlorfenvinphos on the plasma and liver aminotransferases activity - Autorzy:
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Maciejewska-Kozak, H
Szczepaniak, S.
Jeleniewicz, K. - Powiązania:
- https://bibliotekanauki.pl/articles/876377.pdf
- Data publikacji:
- 1992
- Wydawca:
- Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
- Tematy:
-
osocze
aktywnosc enzymatyczna
toksykologia
enzymy
metabolizm czlowieka
watroba
aminotransferazy
szczury
zatrucia przemyslowe
krew
choroby czlowieka
chlorfenwinfos
zwierzeta doswiadczalne
metabolizm
aminokwasy
plasma
enzyme activity
toxicology
enzyme
human metabolism
liver
aminotransferase
rat
industrial poisoning
blood
human disease
chlorfenvinphos
experimental animal
metabolism
amino acid - Opis:
-
W badaniach in vitro wykazano, że chlorfenwinfos w dawce 8,5 µg/cm3 osocza lub homogenatu wątrobowego szczura nie wykazuje istotnego wpływu na aktywność aminotransferaz w osoczu i w cytozolu wątrobowym.
In vitro experiments the effect of chlorfenvinphos on aminotransferases activities in rat plasma and liver homogenate cytoplasmic fraction was studied. As amino groups donors in the transamination reactions with plasma enzymes the next eight amino acids were used: alanine, aspartic acid, cysteine, phenylalanine, leucine, lysine, valine and asparagine, and with the liver cytoplasmatic enzymes - the same above mentioned without asparagine. In all these reactions as an amino groups acceptor alfa-ketoglutaric acid was used. To the incubation mixtures were added: 1 cm3 of the plasma or liver homogenate catoplasmic fraction; 0,05 cm3 of chlorfenvinphos solution in ethyl acetate (0,17 mg/cm3) or 0,05 cm3 ethyl acetate. Aminotransferase activity was expressed as the amount of glutamate developing durign 1 h incubation. Glutamic acid was determined spectrophotometrically after chromatographic separation on filter paper. Both in rat plasma and in liver cytoplasmic fraction all used amino acids as amino groups donors in the transamination reactions were shown. In the reactions with the blood plasma enzymes the most active donors were: alanine, aspartic acid and cysteine and with the participation of liver transaminases: alanine, aspartic acid and phenylalanine. As well in plasma, as in liver the greater acitivity of AIAT than of AspAT was observed. In the reaction with alanine and aspartate there was formed in the case of plasma 1,51 µmol Glu/cm3 and 1,00 µmola Glu/cm3 respectively and in the case of liver - 69,07 µmol Glu/g and 53,26 µmol Glu/g respectively. Results of statistical analysis revealed that small plasma and liver aminotransferases inhibition was caused by the solvent, while the insecticide under test had no influence on the efficiency of transamination. - Źródło:
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Roczniki Państwowego Zakładu Higieny; 1992, 43, 1; 67-73
0035-7715 - Pojawia się w:
- Roczniki Państwowego Zakładu Higieny
- Dostawca treści:
- Biblioteka Nauki
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