- Tytuł:
- Insertion of GPI-anchored alkaline phosphatase into supported membranes: a combined AFM and fluorescence microscopy study.
- Autorzy:
-
Rieu, Jean-Paul
Ronzon, Frédéric
Place, Christophe
Dekkiche, Fairouz
Cross, Benjamin
Roux, Bernard - Powiązania:
- https://bibliotekanauki.pl/articles/1043341.pdf
- Data publikacji:
- 2004
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
fluorescent beads
atomic force microscopy (AFM)
GPI anchors
alkaline phosphatase
supported lipid membranes - Opis:
- A new method based on combined atomic force microscopy (AFM) and fluorescence microscopy observations, is proposed to visualize the insertion of glycosylphosphatidyl inositol (GPI) anchored alkaline phosphatase from buffer solutions into supported phospholipid bilayers. The technique involves the use of 27 nm diameter fluorescent latex beads covalently coupled to the amine groups of proteins. Fluorescence microscopy allows the estimation of the relative protein coverage into the membrane and also introduces a height amplification for the detection of protein/bead complexes with the AFM. The coupling of the beads with the amine groups is not specific; this new and simple approach opens up new ways to investigate proteins into supported membrane systems.
- Źródło:
-
Acta Biochimica Polonica; 2004, 51, 1; 189-197
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł