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    Wyświetlanie 1-2 z 2
    Tytuł:
    Effect of exogenous abscisic acid on accumulation of raffinose family oligosaccharides and galactosyl cyclitols in tiny vetch seeds [Vicia hirsuta [L.] S.F.Gray]
    Autorzy:
    Lahuta, L B
    Gorecki, R.J.
    Gojlo, E.
    Horbowicz, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/57525.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    abscisic acid
    vetch
    oligosaccharide
    botany
    raffinose
    Vicia hirsuta
    galactosyl cyclitol
    seed
    Opis:
    The role of the abscisic acid (ABA) in biosynthesis of raffinose family oligosaccharides (RFOs) and galactosyl cyclitols (Gal-C) in tiny vetch (Vicia hirsuta [L.] S.F. Gray) seeds was investigated. The ABA was applied through incubation of seed at various stage of its development. The level of RFOs and Gal-C was determined in seed maturing on plant and in seed maturing in vitro. In early stages of V. hirsuta seed development, the ABA activated the biosynthesis of galactinol, although the level of arisen galactinol quickly declined. In the later stages of V. hirsuta seed development ABA had stimulatory effect of RFOs and Gal-C biosynthesis. Influence of ABA on biosynthesis of a-galactosides in Vicia hirsuta seed seems to be dependent on abscisic acid concentration. Low concentration of ABA had stimulatory effect on a-galactosides biosynthesis, but high concentration of ABA inhibited the process.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2004, 73, 4
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Exogenously applied D-pinitol and D-chiro-inositol modifies the accumulation of alpha-D-galactosides in developing tiny vetch [Vicia hirsuta [L.] S.F.Gray] seeds
    Autorzy:
    Lahuta, L B
    Horbowicz, M.
    Gojlo, E.
    Goszczynska, J.
    Gorecki, R.J.
    Powiązania:
    https://bibliotekanauki.pl/articles/56915.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    stachyose
    plant physiology
    D-chiro-inositol
    D-pinitol
    alpha-D-galactoside
    raffinose
    Vicia hirsuta
    galactinol synthase
    tiny vetch
    verbascose
    seed
    Opis:
    In the present study we have investigated the effect of exogenous cyclitols on the accumulation of their galactosides and raffinose family oligosaccharides (RFOs), as well as on some enzymes important for their biosynthesis in seeds of tiny vetch (Vicia hirsuta [L.] S.F. Gray). Immature seeds during 6-day incubation with D-chiro-inositol (naturally does not appear in seeds of tiny vetch) were accumulated cyclitol and its galactosides (fagopyritols: B1 and B2). Short 4-hour incubation with D-chiro-inositol, and subsequent slow desiccation process caused accumulation of free cyclitol only, without biosynthesis of its galactosides. Feeding D-chiro-inositol to pods of tiny vetch induced accumulation of high levels of its galactosides (fagopyritol B1, B2 and B3) in maturing seeds. Similarly, feeding D-pinitol increased accumulation of its mono-, di- and tri-galactosides: GPA, GPB, DGPA and TGPA in tiny vetch seed. Accumulation of both cyclitols and their galactosides drastically reduced accumulation of verbascose. Inhibition of RFOs biosynthesis by elevated levels of free cyclitols suggests some competition between formation of both types of galactosides and similarity of both biosynthetic routes in tiny vetch seeds. Galactinol synthase (GolS) from tiny vetch seeds demonstrated ability to utilize D-chiro-inositol as galactosyl acceptor, instead of myo-inositol. Presence of both cyclitols, as substrates for GolS, caused synthesis of their galactosides: fagopyritol B1 and galactinol. However, formation of galactinol was more efficient than fagopyritol B1. D-chiro-Inositol and D-pinitol at concentrations several-fold higher than myo-inositol had inhibitory effect on GolS. Thus, we suggest that a level of free cyclitols can have an influence on the rate of galactinol biosynthesis and further accumulation of RFOs and galactosyl cyclitols in tiny vetch seeds.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2005, 74, 4
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-2 z 2

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