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Wyświetlanie 1-5 z 5
Tytuł:
Zwierzęta wolno żyjace i domowe jako stale utrzymujacy się rezerwuar włośnicy w Polsce
Wild and domestic animals as permanent Trichinella reservoir in Poland
Autorzy:
Cabaj, W.
Powiązania:
https://bibliotekanauki.pl/articles/2144339.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
Polska
pasozyty
nicienie
Warszawa konferencja
zwierzeta dziko zyjace
zywiciele
Trichinella britovi
wystepowanie
wlosien krety
choroby czlowieka
zwierzeta domowe
parazytologia
Trichinella spiralis
konferencje
Opis:
Backround. Since Owen first described Trichinella as a human patogen in 1835, the number of organisms comprising this genus has grown dramatically. This etiological agent of human trichinellosis shows worldwide distribution in domestic and/or sylvatic animals. Material and method. The aim of the presented paper was to determine the distribution of Trichinella species in wild animals such as red foxes, wolves, wild boars, and domestic pigs in Poland. Muscle samples from diaphragm and forelegs were collected from animals killed by hunters. Muscle larvae were recovered from the muscle after artificial digestion and identified at the species level by RAPD, PCR-RPLF and multiplex PCR. Results. Of 75 nematode isolates from red foxes: (Vulpes vulpes), 50 resulted as T. britovi, 6 T. spiralis, 6 were mixed infections of these two species. Fifteen Trichinella isolates remained unidentified. Of 97 nematode isolates from wild boars (Sus scrofa), 21 resulted as T. britovi, 69 T. spiralis, 2 were mixed infections of these two species. Five Trichinella isolates remained unidentified. Of 6 examined wolves (Canis lupus) killed in the Bieszczady region, 3 animals were positive against T. britovi. Of 6 examined raccon dogs (Nyctereutes procyonoides) from Pomorskie region, 2 animals were positive against T. spiralis. Of 21 nematode isolates from domestic pigs, 1 resulted as T. britovi and 21 as T. spiralis. Up to date, two Trichinella species are detected as the etiological agents of epidemiology among domestic and wildlife animal in Poland: T. britovi is the dominant species in red foxes and T. spiralis is the dominant species in wild boars and domestic pigs.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 175-179
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zwierzeta wolno zyjace i domowe jako stale utrzymujacy sie rezerwuar wlosnicy w Polsce
Wild and domestic animals as permanent Trichinella reservoir in Poland
Autorzy:
Cabaj, W
Powiązania:
https://bibliotekanauki.pl/articles/836583.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
Polska
pasozyty
nicienie
Warszawa konferencja
zwierzeta dziko zyjace
zywiciele
Trichinella britovi
wystepowanie
wlosien krety
choroby czlowieka
zwierzeta domowe
parazytologia
Trichinella spiralis
konferencje
Opis:
Backround. Since Owen first described Trichinella as a human patogen in 1835, the number of organisms comprising this genus has grown dramatically. This etiological agent of human trichinellosis shows worldwide distribution in domestic and/or sylvatic animals. Material and method. The aim of the presented paper was to determine the distribution of Trichinella species in wild animals such as red foxes, wolves, wild boars, and domestic pigs in Poland. Muscle samples from diaphragm and forelegs were collected from animals killed by hunters. Muscle larvae were recovered from the muscle after artificial digestion and identified at the species level by RAPD, PCR-RPLF and multiplex PCR. Results. Of 75 nematode isolates from red foxes: (Vulpes vulpes), 50 resulted as T. britovi, 6 T. spiralis, 6 were mixed infections of these two species. Fifteen Trichinella isolates remained unidentified. Of 97 nematode isolates from wild boars (Sus scrofa), 21 resulted as T. britovi, 69 T. spiralis, 2 were mixed infections of these two species. Five Trichinella isolates remained unidentified. Of 6 examined wolves (Canis lupus) killed in the Bieszczady region, 3 animals were positive against T. britovi. Of 6 examined raccon dogs (Nyctereutes procyonoides) from Pomorskie region, 2 animals were positive against T. spiralis. Of 21 nematode isolates from domestic pigs, 1 resulted as T. britovi and 21 as T. spiralis. Up to date, two Trichinella species are detected as the etiological agents of epidemiology among domestic and wildlife animal in Poland: T. britovi is the dominant species in red foxes and T. spiralis is the dominant species in wild boars and domestic pigs.
Źródło:
Annals of Parasitology; 2006, 52, 3; 175-179
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The estimation of different ELISA procedures for serodiagnosis of human trichinellosis
Autorzy:
Moskwa, B.
Bień, J.
Cabaj, W.
Korinkova, K.
Koudela, B.
Kacprzak, E.
Stefaniak, J.
Powiązania:
https://bibliotekanauki.pl/articles/2144348.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parasite
human disease
serodiagnosis
diagnosis
trichinellosis
ELISA test
zoonotic disease
ELISA procedure
excretory-secretory antigen
Trichinella spiralis
human outbreak
Opis:
Introduction. The most important confirmative diagnostic test for trichinellosis is the presence of the muscle larvae in a tissue biopsy but this direct method has a low sensitivity of light and moderate infections. The aim of presented study was to compare the usefulness of the results obtained by three ELISA procedures for Trichinella spp. diagnosis in human outbreaks. Materials and methods. All sera (cases and controls) were tested for anti-Trichinella antibodies (immunoglobulin G) using commercially available Novatec KIT and two other ELISA procedures based on excretory-secretory (ES) antigens on Trichinella spiralis muscle larvae. The main differences in ELISA procedures were: the protein concentration in antigen, dilution of human serum samples, conjugate and the time of conjugate incubation. Additional differences were noticed in ES antigen preparation procedures as well as in T. spiralis isolates used in these procedures. Serum samples were obtained from 22 symptomatical patients from Poznań region (West Poland), geographic area where human outbreak had occurred. Control serum samples were obtained from 20 patients from an open population from a non endemic trichinellosis area. Results. The results were analyzed in terms of both: statistical and epidemiological point of view. Linear regression analysis and correlations coefficient r between OD values of total 22 patients obtained in three ELISA procedures were positive and high statistically significant. Three ELISA procedures revealed different cut-off values and positivity rates for outbreak. However, the majority of positive samples were found as positive in three procedures, but some of them were positive in two or one procedure only. These individual variability in sera reactivity observed in three ELISA procedures could be very important from epidemiological point of view.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 231-238
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The estimation of different ELISA procedures for serodiagnosis of human trichinellosis
Autorzy:
Moskwa, B
Bien, J.
Cabaj, W.
Korinkova, K.
Koudela, B.
Kacprzak, E.
Stefaniak, J.
Powiązania:
https://bibliotekanauki.pl/articles/839966.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parasite
human disease
serodiagnosis
diagnosis
trichinellosis
ELISA test
zoonotic disease
ELISA procedure
excretory-secretory antigen
Trichinella spiralis
human outbreak
Opis:
Introduction. The most important confirmative diagnostic test for trichinellosis is the presence of the muscle larvae in a tissue biopsy but this direct method has a low sensitivity of light and moderate infections. The aim of presented study was to compare the usefulness of the results obtained by three ELISA procedures for Trichinella spp. diagnosis in human outbreaks. Materials and methods. All sera (cases and controls) were tested for anti-Trichinella antibodies (immunoglobulin G) using commercially available Novatec KIT and two other ELISA procedures based on excretory-secretory (ES) antigens on Trichinella spiralis muscle larvae. The main differences in ELISA procedures were: the protein concentration in antigen, dilution of human serum samples, conjugate and the time of conjugate incubation. Additional differences were noticed in ES antigen preparation procedures as well as in T. spiralis isolates used in these procedures. Serum samples were obtained from 22 symptomatical patients from Poznań region (West Poland), geographic area where human outbreak had occurred. Control serum samples were obtained from 20 patients from an open population from a non endemic trichinellosis area. Results. The results were analyzed in terms of both: statistical and epidemiological point of view. Linear regression analysis and correlations coefficient r between OD values of total 22 patients obtained in three ELISA procedures were positive and high statistically significant. Three ELISA procedures revealed different cut-off values and positivity rates for outbreak. However, the majority of positive samples were found as positive in three procedures, but some of them were positive in two or one procedure only. These individual variability in sera reactivity observed in three ELISA procedures could be very important from epidemiological point of view.
Źródło:
Annals of Parasitology; 2006, 52, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-5 z 5

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