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    Wyświetlanie 1-8 z 8
    Tytuł:
    Badanie przydatnosci testu ELISA do wczesnego diagnozowania inwazji z rodzaju Trichinella u swin
    The usefulness of ELISA test early serological detection of Trichinella spp. infection in pigs
    Autorzy:
    Bien, J
    Powiązania:
    https://bibliotekanauki.pl/articles/838974.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    diagnostyka wczesna
    pasozyty zwierzat
    trzoda chlewna
    Trichinella spiralis
    parazytologia
    inwazja pasozytnicza
    przydatnosc
    test ELISA
    Źródło:
    Annals of Parasitology; 2007, 53, 2; 149-151
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wpływ procedury otrzymywania antygenów ekskrecyjno-sekrecyjnych Trichinella spiralis na wynik testu ELISA u zarażonych świń
    The influence of the procedure of excretory -secretory L1 Trichinella spiralis antigen preparation on the efficiency of an ELISA test in pigs
    Autorzy:
    Bień, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2144343.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    analiza statystyczna
    diagnostyka weterynaryjna
    pasozyty
    nicienie
    Warszawa konferencja
    otrzymywanie
    procedury
    trzoda chlewna
    zwierzeta chore
    parazytologia
    Trichinella spiralis
    konferencje
    antygeny
    wartosc diagnostyczna
    test ELISA
    Opis:
    Background. Trichinellosis is a parasitic zoonosis transmitted to humans by consumption of raw or undercooked meat from animals infected by worms of the Trichinella genus. Every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease in Poland. The usefulness of ELISA for anti-T. spiralis IgG detection in pigs is still limited by the nature of antigen. The objective in the present study was to compare the usefulness of excretory-secretory antigens of L1 T. spiralis for the serological detection of IgG antibodies in pigs. Material and methods. The antigens were prepared in different laboratories: Ag ES L1 T. spiralis (N) in Germany, Ag ES L1 T. spiralis (W) in Italy and Ag ES L1 T. spiralis in Poland. Conventional, Iberian pigs were infected with 200, 1000 and 20 000 muscle larvae of T. spiralis. Serum samples were obtained at 5 and 1 dbi (day before infection), and 5, 10, 15, 20, 25, 30, 40, 50, 60 dpi (day post infection) and screened for specific IgG antibodies to excretory-secretory L1 T. spiralis antigens. Serum samples were obtained from the EU project TRICHIPORSE. The cut-off value of ELISA was determined on serum samples from 248 Trichinella-free pigs from Poznań and Boża Wola, that were examined by artificial digestion. Results. In pigs infected with 200 L1 T. spiralis larvae, specific IgG were detectable from 50 dpi, when the Ag ES L1 T. spiralis (N) was used, whereas when Ag ES L1T. spiralis (W) and Ag ES L1 T. spiralis were used, the specific IgG were detectable from 40 dpi. In pigs infected with 1000 L1 T. spiralis larvae, specific IgG was observed from 30 dpi when Ag ES L1 T. spiralis (W) and Ag ES L1 T. spiralis were used, but when Ag ES L1 T. spiralis (N) was used specific IgG were detectable from 40 dpi. In the group infected with the highest dose of T. spiralis larvae, specific IgG were detectable from 30 dpi when Ag ES L1 T. spiralis (N) and Ag ES L1 T. spiralis (W) were used, whereas when Ag ES L1 T. spiralis was used specific IgG were detectable from 20 dpi. The results strongly indicated that in the examined pigs, the specific IgG response against T. spiralis infection is dose dependent. Furthermore, it was shown that the high infectious dose induced earlier increasing of specific IgG response. Statistical analysis revealed a significant positive correlation between OD values obtained in procedures based on the three antigens. The results were statistically repeatable for procedures and for single pigs (P<0.01).
    Źródło:
    Wiadomości Parazytologiczne; 2006, 52, 3; 219-230
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Badanie przydatności testu ELISA do wczesnego diagnozowania inwazji z rodzaju Trichinella u świń
    The usefulness of ELISA test early serological detection of Trichinella spp. infection in pigs
    Autorzy:
    Bień, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143857.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    diagnostyka wczesna
    pasozyty zwierzat
    trzoda chlewna
    Trichinella spiralis
    parazytologia
    inwazja pasozytnicza
    przydatnosc
    test ELISA
    Opis:
    Trichinellosis is a parasitic zoonosis transmitted to humans through consumption of raw or undercooked meat from animals infected with nematodes of the Trichinella genus. Every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease. The first step of the study was the optimization of a new ELISA method enabling an early and specific serological diagnosis of trichinellosis in pigs and wild boars using excretory−secretory (ES) antigens obtained from in vitro cultures of L1 T. spiralis. Serum samples were assayed for anti−T. spiralis IgG antibodies using the new ELISA protocol and a reference test — Standard manufactured by Institut Pourquier. The optimization involved the selection of suitable plates for antigen coating, dilution of sera and antibodies and their time of incubation. On the basis of the optimization a new ELISA procedure for the detection of IgG and IgM against T. spiralis was elaborated. Conventional, Iberian pigs and SPF (Specific Pathogen Free) pigs were infected with 200, 1000 and 20,000 muscle larvae of T. spiralis. Serum samples were obtained at 5 and 1 dbi (day before infection), and 5, 10, 15, 20, 25, 30, 40, 50, 60 dpi (day post infection) and screened for specific IgG antibodies against excretory−secretory L1 T. spiralis antigens. Serum samples were obtained from the EU project Trichiporse: „Safe pork and horse meat on EU markets: early and unbiased diagnostic tests for Trichinella”. Field samples of conventional pigs (1474) and wild boars (1784) were obtained from slaughter houses in different parts of Poland. Pigs were examined for the presence of Trichinella spp. using the artificial digestion method. Only four pigs were naturally infected with T. spiralis, the remaining were Trichinella larvae free. ELISA was used to examine IgG levels against L1 T. spiralis in pig and wild boar sera. The usefulness of ELISA for anti−IgG detection in pigs is usually limited by the nature of the antigen. The antigens were prepared in different laboratories: in Germany — Ag ES L1 T. spiralis (N), Italy — Ag ES L1 T. spiralis (W)) and in Poland — Ag ES L1 T. spiralis. Cut−off values for ELISA along with the estimated sensitivity and specificity were calculated using different methods: S/P%, M+3SD and ROC (Receiver Operating Characteristic). In SPF and Iberian pigs inoculated with 200, 1000 and 20,000 L1 T. spiralis, specific antibodies were detected 40, 30 and 25 dpi, respectively, with the use of the Standard (reference test). The analysis of the two ELISA procedures demonstrated a high sensitivity and specificity for the newly elaborated test utilizing the Ag ES L1 T. spiralis. In conventional pigs infected with 20,000 L1 T. spiralis specific antibodies were detected from 20 dpi when employing the new protocol. Similar results for the Standard and new ELISA test were obtained for serum samples of conventional pigs infected with 200 and 1000 larvae, which became positive from 40 dpi and 30 dpi, respectively. The results showed that both: the Standard and new protocols were comparable, and based on this, the new test was applied for further research. Results obtained adopting the new protocol with three antigens showed that two of them: Ag ES L1 T. spiralis (W) and Ag ES L1 T. spiralis are similar. The specific IgG antibodies for infective doses of 200 and 1000 larvae for these antigens were detectable 40 and 30 dpi respectively. In pigs infected with the highest dose of T. spiralis larvae IgG anti bodies were detectable from 20 dpi when Ag ES L1 T. spiralis was used. These results strongly indicate that in examined pigs, the specific IgG response to T. spiralis infection is dose dependant. Of 1474 examined pig sera only 0.99% gave a positive signal against ES L1 T. spiralis antigen. Of 1784 examined wild boars sera only 0.68 % gave positive results using the new ELISA protocol. ELISA is a useful method for detecting specific IgG antibodies in pigs experimentally infected with different doses of T. spiralis and naturally infected pigs. In pigs the specific IgG response is dose dependant. The Ag ES L1 T. spiralis increases the specifity of the method and reduces false positive results. Simultaneous use of both methods: digestion and ELISA for the diagnosis of Trichinella in naturally infected pigs and wild boars may increase the chances of eliminating meat infected with T. spiralis larvae.
    Źródło:
    Wiadomości Parazytologiczne; 2007, 53, 2; 149-151
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wplyw procedury otrzymywania antygenow ekskrecyjno-sekrecyjnych Trichinella spiralis na wynik testu ELISA u zarazonych swin
    The influence of the procedure of excretory -secretory L1 Trichinella spiralis antigen preparation on the efficiency of an ELISA test in pigs
    Autorzy:
    Bien, J
    Powiązania:
    https://bibliotekanauki.pl/articles/840241.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    analiza statystyczna
    diagnostyka weterynaryjna
    pasozyty
    nicienie
    Warszawa konferencja
    otrzymywanie
    procedury
    trzoda chlewna
    zwierzeta chore
    parazytologia
    Trichinella spiralis
    konferencje
    antygeny
    wartosc diagnostyczna
    test ELISA
    Opis:
    Background. Trichinellosis is a parasitic zoonosis transmitted to humans by consumption of raw or undercooked meat from animals infected by worms of the Trichinella genus. Every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease in Poland. The usefulness of ELISA for anti-T. spiralis IgG detection in pigs is still limited by the nature of antigen. The objective in the present study was to compare the usefulness of excretory-secretory antigens of L1 T. spiralis for the serological detection of IgG antibodies in pigs. Material and methods. The antigens were prepared in different laboratories: Ag ES L1 T. spiralis (N) in Germany, Ag ES L1 T. spiralis (W) in Italy and Ag ES L1 T. spiralis in Poland. Conventional, Iberian pigs were infected with 200, 1000 and 20 000 muscle larvae of T. spiralis. Serum samples were obtained at 5 and 1 dbi (day before infection), and 5, 10, 15, 20, 25, 30, 40, 50, 60 dpi (day post infection) and screened for specific IgG antibodies to excretory-secretory L1 T. spiralis antigens. Serum samples were obtained from the EU project TRICHIPORSE. The cut-off value of ELISA was determined on serum samples from 248 Trichinella-free pigs from Poznań and Boża Wola, that were examined by artificial digestion. Results. In pigs infected with 200 L1 T. spiralis larvae, specific IgG were detectable from 50 dpi, when the Ag ES L1 T. spiralis (N) was used, whereas when Ag ES L1T. spiralis (W) and Ag ES L1 T. spiralis were used, the specific IgG were detectable from 40 dpi. In pigs infected with 1000 L1 T. spiralis larvae, specific IgG was observed from 30 dpi when Ag ES L1 T. spiralis (W) and Ag ES L1 T. spiralis were used, but when Ag ES L1 T. spiralis (N) was used specific IgG were detectable from 40 dpi. In the group infected with the highest dose of T. spiralis larvae, specific IgG were detectable from 30 dpi when Ag ES L1 T. spiralis (N) and Ag ES L1 T. spiralis (W) were used, whereas when Ag ES L1 T. spiralis was used specific IgG were detectable from 20 dpi. The results strongly indicated that in the examined pigs, the specific IgG response against T. spiralis infection is dose dependent. Furthermore, it was shown that the high infectious dose induced earlier increasing of specific IgG response. Statistical analysis revealed a significant positive correlation between OD values obtained in procedures based on the three antigens. The results were statistically repeatable for procedures and for single pigs (P<0.01).
    Źródło:
    Annals of Parasitology; 2006, 52, 3; 219-230
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Anti-Trichinella antibodies in the meat juice of different species of carnivores
    Autorzy:
    Cybulska, A.
    Bien, J.
    Moskwa, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/5854.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    anti-Trichinella antibody
    antibody
    nematode
    Trichinella
    Trichinella spiralis
    Trichinella britovi
    Trichinella nativa
    Trichinella pseudospiralis
    meat juice
    carnivore
    Źródło:
    Annals of Parasitology; 2016, 62, Suppl.
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    1D-immunoblot as a species-specific diagnostic tool in human trichinellosis: yes or no?
    Autorzy:
    Bien, J.
    Gozdzik, K.
    Moskwa, B.
    Stefaniak, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/6700.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    immunoblotting
    diagnostic method
    Trichinella spiralis
    etiological agent
    human infection
    human trichinellosis
    trichinellosis
    Źródło:
    Annals of Parasitology; 2016, 62, Suppl.
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The estimation of different ELISA procedures for serodiagnosis of human trichinellosis
    Autorzy:
    Moskwa, B.
    Bień, J.
    Cabaj, W.
    Korinkova, K.
    Koudela, B.
    Kacprzak, E.
    Stefaniak, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2144348.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasite
    human disease
    serodiagnosis
    diagnosis
    trichinellosis
    ELISA test
    zoonotic disease
    ELISA procedure
    excretory-secretory antigen
    Trichinella spiralis
    human outbreak
    Opis:
    Introduction. The most important confirmative diagnostic test for trichinellosis is the presence of the muscle larvae in a tissue biopsy but this direct method has a low sensitivity of light and moderate infections. The aim of presented study was to compare the usefulness of the results obtained by three ELISA procedures for Trichinella spp. diagnosis in human outbreaks. Materials and methods. All sera (cases and controls) were tested for anti-Trichinella antibodies (immunoglobulin G) using commercially available Novatec KIT and two other ELISA procedures based on excretory-secretory (ES) antigens on Trichinella spiralis muscle larvae. The main differences in ELISA procedures were: the protein concentration in antigen, dilution of human serum samples, conjugate and the time of conjugate incubation. Additional differences were noticed in ES antigen preparation procedures as well as in T. spiralis isolates used in these procedures. Serum samples were obtained from 22 symptomatical patients from Poznań region (West Poland), geographic area where human outbreak had occurred. Control serum samples were obtained from 20 patients from an open population from a non endemic trichinellosis area. Results. The results were analyzed in terms of both: statistical and epidemiological point of view. Linear regression analysis and correlations coefficient r between OD values of total 22 patients obtained in three ELISA procedures were positive and high statistically significant. Three ELISA procedures revealed different cut-off values and positivity rates for outbreak. However, the majority of positive samples were found as positive in three procedures, but some of them were positive in two or one procedure only. These individual variability in sera reactivity observed in three ELISA procedures could be very important from epidemiological point of view.
    Źródło:
    Wiadomości Parazytologiczne; 2006, 52, 3; 231-238
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The estimation of different ELISA procedures for serodiagnosis of human trichinellosis
    Autorzy:
    Moskwa, B
    Bien, J.
    Cabaj, W.
    Korinkova, K.
    Koudela, B.
    Kacprzak, E.
    Stefaniak, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/839966.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasite
    human disease
    serodiagnosis
    diagnosis
    trichinellosis
    ELISA test
    zoonotic disease
    ELISA procedure
    excretory-secretory antigen
    Trichinella spiralis
    human outbreak
    Opis:
    Introduction. The most important confirmative diagnostic test for trichinellosis is the presence of the muscle larvae in a tissue biopsy but this direct method has a low sensitivity of light and moderate infections. The aim of presented study was to compare the usefulness of the results obtained by three ELISA procedures for Trichinella spp. diagnosis in human outbreaks. Materials and methods. All sera (cases and controls) were tested for anti-Trichinella antibodies (immunoglobulin G) using commercially available Novatec KIT and two other ELISA procedures based on excretory-secretory (ES) antigens on Trichinella spiralis muscle larvae. The main differences in ELISA procedures were: the protein concentration in antigen, dilution of human serum samples, conjugate and the time of conjugate incubation. Additional differences were noticed in ES antigen preparation procedures as well as in T. spiralis isolates used in these procedures. Serum samples were obtained from 22 symptomatical patients from Poznań region (West Poland), geographic area where human outbreak had occurred. Control serum samples were obtained from 20 patients from an open population from a non endemic trichinellosis area. Results. The results were analyzed in terms of both: statistical and epidemiological point of view. Linear regression analysis and correlations coefficient r between OD values of total 22 patients obtained in three ELISA procedures were positive and high statistically significant. Three ELISA procedures revealed different cut-off values and positivity rates for outbreak. However, the majority of positive samples were found as positive in three procedures, but some of them were positive in two or one procedure only. These individual variability in sera reactivity observed in three ELISA procedures could be very important from epidemiological point of view.
    Źródło:
    Annals of Parasitology; 2006, 52, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-8 z 8

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